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The map label for this gene is infC

Identifier: 209551835

GI number: 209551835

Start: 4439128

End: 4439664

Strand: Direct

Name: infC

Synonym: Rleg2_4267

Alternate gene names: 209551835

Gene position: 4439128-4439664 (Clockwise)

Preceding gene: 209551832

Following gene: 209551836

Centisome position: 97.82

GC content: 57.17

Gene sequence:

>537_bases
ATTCGCAGACCTTTTAAAACCGACGCGCCCGTGAAGGACGGACCGCGCTCGAACCGTGAAATCCGCATTCCCAAAGTTCA
GCTGATCGGGGCTGACGGCGAGAATATGGGCGTCGTGCCTACCGACCAGGCCTTGAGAATGGCTGAGGAAGCCGGCCTCG
ATCTCGTCGAAATTTCCCCCAATGTCGAACCGCCTGTGTGCAAGATCCTCGATCTGGGCAAGCTGAAATATGCCAACCAG
AAGAAGGCTGCCGAGGCGCGCAAGAAGCAGAAGATCGTCGAAGTCAAAGAAATCAAGATGCGCCCGAACATCGACACCCA
TGATTATGAGGTGAAGATGAAGGCGATGGGTCGCTTCTTCGACGAAGGCGACAAGGTCAAGGTGACGCTGAAGTTCCGCG
GCCGCGAAATGGCCCACCAGGAACTCGGCATGAAGCTGCTGCAGCAGGTCAAGGCCGATACGACCGAGTTCGCCAAGGTC
GAAGCCGAGCCCAAGCTCGAAGGCCGTCAGATGATGATGGTGCTGGCGCCGAAGTAA

Upstream 100 bases:

>100_bases
GATATTGACTCCGTTGCAGCGATGACGCGACACGTTTGTGCACCCCGATATGGGAGCGCGAGGCTGCAACGTTCCGAAAC
AACGCGAGGAGAATACGACC

Downstream 100 bases:

>100_bases
GCGCCAGGCACTTTTTCGCCCCAAGGCCGTCCGCAAGGGCGGCTTTTGTGCTTTCTGACACTGGTGCTTACGAGCGTGCG
AAGATTCCTCCGCAGCCCCG

Product: translation initiation factor IF-3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MRRPFKTDAPVKDGPRSNREIRIPKVQLIGADGENMGVVPTDQALRMAEEAGLDLVEISPNVEPPVCKILDLGKLKYANQ
KKAAEARKKQKIVEVKEIKMRPNIDTHDYEVKMKAMGRFFDEGDKVKVTLKFRGREMAHQELGMKLLQQVKADTTEFAKV
EAEPKLEGRQMMMVLAPK

Sequences:

>Translated_178_residues
MRRPFKTDAPVKDGPRSNREIRIPKVQLIGADGENMGVVPTDQALRMAEEAGLDLVEISPNVEPPVCKILDLGKLKYANQ
KKAAEARKKQKIVEVKEIKMRPNIDTHDYEVKMKAMGRFFDEGDKVKVTLKFRGREMAHQELGMKLLQQVKADTTEFAKV
EAEPKLEGRQMMMVLAPK
>Mature_178_residues
MRRPFKTDAPVKDGPRSNREIRIPKVQLIGADGENMGVVPTDQALRMAEEAGLDLVEISPNVEPPVCKILDLGKLKYANQ
KKAAEARKKQKIVEVKEIKMRPNIDTHDYEVKMKAMGRFFDEGDKVKVTLKFRGREMAHQELGMKLLQQVKADTTEFAKV
EAEPKLEGRQMMMVLAPK

Specific function: IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins

COG id: COG0290

COG function: function code J; Translation initiation factor 3 (IF-3)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-3 family

Homologues:

Organism=Escherichia coli, GI1788012, Length=164, Percent_Identity=58.5365853658537, Blast_Score=184, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IF3_RHILW (B5ZXR7)

Other databases:

- EMBL:   CP001191
- RefSeq:   YP_002283752.1
- GeneID:   6983041
- GenomeReviews:   CP001191_GR
- KEGG:   rlt:Rleg2_4267
- HOGENOM:   HBG744268
- OMA:   KQMKFRL
- ProtClustDB:   PRK00028
- GO:   GO:0005737
- HAMAP:   MF_00080
- InterPro:   IPR001288
- InterPro:   IPR019815
- InterPro:   IPR019814
- Gene3D:   G3DSA:3.10.20.80
- Gene3D:   G3DSA:3.30.110.10
- TIGRFAMs:   TIGR00168

Pfam domain/function: PF00707 IF3_C; PF05198 IF3_N; SSF54364 IF3; SSF55200 IF3

EC number: NA

Molecular weight: Translated: 20186; Mature: 20186

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS00938 IF3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPFKTDAPVKDGPRSNREIRIPKVQLIGADGENMGVVPTDQALRMAEEAGLDLVEISP
CCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEECC
NVEPPVCKILDLGKLKYANQKKAAEARKKQKIVEVKEIKMRPNIDTHDYEVKMKAMGRFF
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEHHHHHHHC
DEGDKVKVTLKFRGREMAHQELGMKLLQQVKADTTEFAKVEAEPKLEGRQMMMVLAPK
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHEECCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MRRPFKTDAPVKDGPRSNREIRIPKVQLIGADGENMGVVPTDQALRMAEEAGLDLVEISP
CCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEECC
NVEPPVCKILDLGKLKYANQKKAAEARKKQKIVEVKEIKMRPNIDTHDYEVKMKAMGRFF
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEHHHHHHHC
DEGDKVKVTLKFRGREMAHQELGMKLLQQVKADTTEFAKVEAEPKLEGRQMMMVLAPK
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHEECCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA