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The map label for this gene is 209551783

Identifier: 209551783

GI number: 209551783

Start: 4389852

End: 4390610

Strand: Reverse

Name: 209551783

Synonym: Rleg2_4212

Alternate gene names: NA

Gene position: 4390610-4389852 (Counterclockwise)

Preceding gene: 209551784

Following gene: 209551780

Centisome position: 96.75

GC content: 59.03

Gene sequence:

>759_bases
ATGACCACGAAGGACGATCGTCTCGAATTCGAAGCCCACAAGCGGGAAATGTCGCTTGCGCTCGGCAAGGATGAGGCAAG
TTTCCAACAATCGCTGAACACACTGATCGGGCTCGATAAGTTCGACTATTCCTATCTCTGGTCATGGATGGGCGTGCCTA
TCATCCAGCTTCCGGTCGATATCCTGGCAACCCAGGAAGTCATCTGGGCGACCAAGCCCGATGTCATCATCGAAACCGGC
ATCGCGCGCGGCGGCTCGCTAATCTTCATGGCGTCGATCCTCGCTGCCATGGGCAACGACGCGGCCAAGGTTGTCGGCGT
CGACATCGACATCCGCGCCCATAATCGCGAGTCGATCGAATCCCATCCGATGTCGAGCCGGATCAAGATGATCCAGGGCG
GTTCCGTCGACGACGAGGTGCTTGCTGCCGTGAAAGCCGAGATCCCGCCGGGCGCCCGTGTGATGGTAGTGCTCGACAGC
GACCATTCCTACGAGCATGTTCTGGCGGAATGCCGCGCCTACGGTCCGCTGGTGACGGAAGGTTGCTACTTGGTCGTCGC
CGACACGTTCATCGGTCACCTGAGCGAAGAACAGGCTTTCACAAAACGTTCCAAGGTCTGGCTGCGCGGCAATGAGCCGC
TGAAGGCCATCACCGATTATCTGGCGGAAACCGACCGCTTCGAAGTTGATCCGGTTCTGAACGGCAAGCTCGTGCTGTCT
TCCTCGCCGGGCGGTTACTGCGTCTGCCGCAAGACGTGA

Upstream 100 bases:

>100_bases
AACGTTGCTCGATGTGGCGGCAGGGCGGATCTATCACAACAGCAAGGCATTACGGCAGTCGGGCTAAGGCAGCCCCTACA
ACGAAGGACAAGAAACAGTC

Downstream 100 bases:

>100_bases
GCGGACAATCAGGGATCGTCGTTTCGACTATCTGAGGACGGAGTTTCCGATCGAACGTTTCCGGCAAGCGCATCCGGCGG
CTTTGCCGACAGGCCAAAGC

Product: Cephalosporin hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTTKDDRLEFEAHKREMSLALGKDEASFQQSLNTLIGLDKFDYSYLWSWMGVPIIQLPVDILATQEVIWATKPDVIIETG
IARGGSLIFMASILAAMGNDAAKVVGVDIDIRAHNRESIESHPMSSRIKMIQGGSVDDEVLAAVKAEIPPGARVMVVLDS
DHSYEHVLAECRAYGPLVTEGCYLVVADTFIGHLSEEQAFTKRSKVWLRGNEPLKAITDYLAETDRFEVDPVLNGKLVLS
SSPGGYCVCRKT

Sequences:

>Translated_252_residues
MTTKDDRLEFEAHKREMSLALGKDEASFQQSLNTLIGLDKFDYSYLWSWMGVPIIQLPVDILATQEVIWATKPDVIIETG
IARGGSLIFMASILAAMGNDAAKVVGVDIDIRAHNRESIESHPMSSRIKMIQGGSVDDEVLAAVKAEIPPGARVMVVLDS
DHSYEHVLAECRAYGPLVTEGCYLVVADTFIGHLSEEQAFTKRSKVWLRGNEPLKAITDYLAETDRFEVDPVLNGKLVLS
SSPGGYCVCRKT
>Mature_251_residues
TTKDDRLEFEAHKREMSLALGKDEASFQQSLNTLIGLDKFDYSYLWSWMGVPIIQLPVDILATQEVIWATKPDVIIETGI
ARGGSLIFMASILAAMGNDAAKVVGVDIDIRAHNRESIESHPMSSRIKMIQGGSVDDEVLAAVKAEIPPGARVMVVLDSD
HSYEHVLAECRAYGPLVTEGCYLVVADTFIGHLSEEQAFTKRSKVWLRGNEPLKAITDYLAETDRFEVDPVLNGKLVLSS
SPGGYCVCRKT

Specific function: Catalyzes the O-methylation of the hydroxyl group located on C-2 of the first rhamnosyl residue linked to the phenolic group of glycosylated phenolphthiocerol dimycocerosates (PGL) and p-hydroxybenzoic acid derivatives (p-HBAD) [H]

COG id: COG3510

COG function: function code V; Cephalosporin hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rhamnosyl O-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007072 [H]

Pfam domain/function: PF04989 CmcI [H]

EC number: NA

Molecular weight: Translated: 27746; Mature: 27615

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKDDRLEFEAHKREMSLALGKDEASFQQSLNTLIGLDKFDYSYLWSWMGVPIIQLPVD
CCCCCCCCHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEECHH
ILATQEVIWATKPDVIIETGIARGGSLIFMASILAAMGNDAAKVVGVDIDIRAHNRESIE
HHHCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCHH
SHPMSSRIKMIQGGSVDDEVLAAVKAEIPPGARVMVVLDSDHSYEHVLAECRAYGPLVTE
CCCCHHHEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHC
GCYLVVADTFIGHLSEEQAFTKRSKVWLRGNEPLKAITDYLAETDRFEVDPVLNGKLVLS
CEEEEEEHHHHHHCCHHHHHHHHCEEEEECCCCHHHHHHHHHCCCCEEECCCCCCEEEEE
SSPGGYCVCRKT
CCCCCEEEEECC
>Mature Secondary Structure 
TTKDDRLEFEAHKREMSLALGKDEASFQQSLNTLIGLDKFDYSYLWSWMGVPIIQLPVD
CCCCCCCHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEECHH
ILATQEVIWATKPDVIIETGIARGGSLIFMASILAAMGNDAAKVVGVDIDIRAHNRESIE
HHHCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCHH
SHPMSSRIKMIQGGSVDDEVLAAVKAEIPPGARVMVVLDSDHSYEHVLAECRAYGPLVTE
CCCCHHHEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHC
GCYLVVADTFIGHLSEEQAFTKRSKVWLRGNEPLKAITDYLAETDRFEVDPVLNGKLVLS
CEEEEEEHHHHHHCCHHHHHHHHCEEEEECCCCHHHHHHHHHCCCCEEECCCCCCEEEEE
SSPGGYCVCRKT
CCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]