Click here to switch to the map view.

The map label for this gene is gsiD [H]

Identifier: 209551770

GI number: 209551770

Start: 4373199

End: 4374059

Strand: Reverse

Name: gsiD [H]

Synonym: Rleg2_4199

Alternate gene names: 209551770

Gene position: 4374059-4373199 (Counterclockwise)

Preceding gene: 209551771

Following gene: 209551766

Centisome position: 96.39

GC content: 63.65

Gene sequence:

>861_bases
ATGGCTCCCGTCGTTTCCAGCGCCTTTGCCAGCCGCATAGTTTTTAAACTTAGCCGGAGAATGAACCTGGTTTGCGGGGC
GGTGATTATCGGGCTGCTTATCGCCGTTGCACTGCTGTCGCTGGTCTGGACGCCACTGCCGCCGGCAAAAATGCAGATCA
TTCACAAACTGCAGCCGCCGCTTGCCTTCGGCCTGCTCGGCACCGATCAATTCGGCCGAGACGTGCTGTCGATGATGATG
GCGGGATGCTGGAATTCGCTGTCGATCGCCATCACCGCAGTCGCAGTCGGCGGGACGCTCGGCTCGATCGCCGGCATTTC
GGCAGCCGCGATCCGCGGCCTTTTCGAAGCGCTGCTGATGCGCATCTGCGACGTCATCTTCGCGCTGCCGCCGATCCTGT
CCGCCATGGTGCTCGGCGCCTTTCTCGGGCCTGGCCGTTTTACCGCAATCACCGCGATCGCCGTCTTCATGATCCCGGTC
TTTGCCCGGGTGACGCTTGCGACTTCGCTGCAGGCCTGGAGCCGCGACTATGTCATGGCGGCGCGCGCGATCGGCAATAC
GCGCCTGACCATTTCGCTGCGCCATGTGCTGCCGAATATCATCAGCCAGATCATCGTCCATGGCGCCATCCAACTGGGGC
TGGCGATCCTGACCGAAGCCGGTCTCAGCTTCCTCGGGCTCGGCTTGGCGCCGCCGGCGCCAAGCTGGGGGCGGATGCTG
GCCGATGCGCAAACCTATCTGGCGCTCGCCCCTTGGCTCGCGATTTTGCCCGGTCTTGCCATCGCACTCGCCGTCTTCGG
TTTCAACATGCTTGGCGACGGCTTGCGCGATCTTCTCGACCCGCGCGAGGCGCGCCGCTGA

Upstream 100 bases:

>100_bases
TGTCGTGCTGTTCTTCGCCGCCCTCGTCATCGTCGTGAATTTCGTGATCGATCTCTCCTATCTGGCGATCGATCCCAGGA
TGAGAAAGGCGGCTTGAGGC

Downstream 100 bases:

>100_bases
TCAGCCGGTCGGGCGGCGGTAGCCGGTCGGCTGTTCGTACAGCCAGCCGATACGCTTGACGGCGGCCTCGAAATTCTCGC
GCGCCACCGTCATGCTGTGG

Product: binding-protein-dependent transporters inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MAPVVSSAFASRIVFKLSRRMNLVCGAVIIGLLIAVALLSLVWTPLPPAKMQIIHKLQPPLAFGLLGTDQFGRDVLSMMM
AGCWNSLSIAITAVAVGGTLGSIAGISAAAIRGLFEALLMRICDVIFALPPILSAMVLGAFLGPGRFTAITAIAVFMIPV
FARVTLATSLQAWSRDYVMAARAIGNTRLTISLRHVLPNIISQIIVHGAIQLGLAILTEAGLSFLGLGLAPPAPSWGRML
ADAQTYLALAPWLAILPGLAIALAVFGFNMLGDGLRDLLDPREARR

Sequences:

>Translated_286_residues
MAPVVSSAFASRIVFKLSRRMNLVCGAVIIGLLIAVALLSLVWTPLPPAKMQIIHKLQPPLAFGLLGTDQFGRDVLSMMM
AGCWNSLSIAITAVAVGGTLGSIAGISAAAIRGLFEALLMRICDVIFALPPILSAMVLGAFLGPGRFTAITAIAVFMIPV
FARVTLATSLQAWSRDYVMAARAIGNTRLTISLRHVLPNIISQIIVHGAIQLGLAILTEAGLSFLGLGLAPPAPSWGRML
ADAQTYLALAPWLAILPGLAIALAVFGFNMLGDGLRDLLDPREARR
>Mature_285_residues
APVVSSAFASRIVFKLSRRMNLVCGAVIIGLLIAVALLSLVWTPLPPAKMQIIHKLQPPLAFGLLGTDQFGRDVLSMMMA
GCWNSLSIAITAVAVGGTLGSIAGISAAAIRGLFEALLMRICDVIFALPPILSAMVLGAFLGPGRFTAITAIAVFMIPVF
ARVTLATSLQAWSRDYVMAARAIGNTRLTISLRHVLPNIISQIIVHGAIQLGLAILTEAGLSFLGLGLAPPAPSWGRMLA
DAQTYLALAPWLAILPGLAIALAVFGFNMLGDGLRDLLDPREARR

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=268, Percent_Identity=39.1791044776119, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1787760, Length=272, Percent_Identity=35.2941176470588, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789964, Length=218, Percent_Identity=41.2844036697248, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789889, Length=268, Percent_Identity=33.955223880597, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787549, Length=212, Percent_Identity=37.2641509433962, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787498, Length=218, Percent_Identity=30.7339449541284, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788505, Length=226, Percent_Identity=28.7610619469027, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30158; Mature: 30027

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
APVVSSAFASRIVFKLSRRMNLVCGAVIIGLLIAVALLSLVWTPLPPAKMQIIHKLQPP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
LAFGLLGTDQFGRDVLSMMMAGCWNSLSIAITAVAVGGTLGSIAGISAAAIRGLFEALLM
HHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RICDVIFALPPILSAMVLGAFLGPGRFTAITAIAVFMIPVFARVTLATSLQAWSRDYVMA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAIGNTRLTISLRHVLPNIISQIIVHGAIQLGLAILTEAGLSFLGLGLAPPAPSWGRML
HHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
ADAQTYLALAPWLAILPGLAIALAVFGFNMLGDGLRDLLDPREARR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]