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The map label for this gene is mepA [H]

Identifier: 209551702

GI number: 209551702

Start: 4310720

End: 4311796

Strand: Reverse

Name: mepA [H]

Synonym: Rleg2_4131

Alternate gene names: 209551702

Gene position: 4311796-4310720 (Counterclockwise)

Preceding gene: 209551710

Following gene: 209551701

Centisome position: 95.02

GC content: 68.62

Gene sequence:

>1077_bases
ATGGCTTTCGGCTTCGCTCAGGCTTTCAGGACATTCGGCAAACTGACGCTTGCTGGGGCAATCGGCGCGAGCCTTGCCGC
CGGCGATGTCGGGGCCGAGCAGAAGACGCCGAGCCCCGGCAGCGCCAAGGGCCAGTTCGGCGCCGTCGGCCTGCCGACGC
AGGGACCGGCGCAGCCGATCGGGTTTTACGCCAAGGGCTGCATGACGGGTGCGGTGGCGCTGCCGGCCGACGGGCCGACC
TGGGAGGCGATGCGGCTTTCGCGCAATCGACGCTGGGGCAATCCGGCGATGATTTCGCTGCTCGAACGCCTTTCACAGGA
TGGGGTCAAATATGCCGGCTGGCCGGGCCTTCTCGTCGGCGACATCGCGCAGCCGCGCGGCGGGCCGATGTTCAACGGTC
ATGCCTCACACCAGATCGGCCTCGATGCCGACGTCTGGTTCACGCCGATGCCGGCGCGCCGCATGACGGCCAAGGAGCGC
GAGGACCTGCCCTTCACCACCATGCTGCAGAAGGACAAGTTCCTGACCGTCGACCCCAATGTGTGGACGCAATCGCGGGC
GCGGCTGCTGATGCTGGCGGCAAGCTATCCCGAGGTGGAGCGCATCTTCGTCAACCCGGCGATCAAGAAAAAGATGTGCG
ACACCTGGACGGGCGACCGCACCAATCTCGGCAAGCTTCGGCCGGAATACGGCCATGACTCGCATTTCCACATCCGCATC
AAATGCCCGGCCGGCGCGGCCGGATGCACGCCGCAGGCGCCTGTCGCCGCCGGCGACGGCTGCGACAAGTCGCTCGCCTG
GTGGTTCACGCCCGCCCCCTGGGCGCCGCCGAAACCGCCGAAGCCCGGCGCCAAGCCGCCGAAGCCGCCGCGCGAGATGA
TGGTTTCCGACCTGCCGAAAGCCTGCGCCGCCGTGCTCGATGCCGCGTCCGTCGCCTCGATGCAGGCCGCCACCTATGGC
GGGCCGTCCGCCGCAAGCGCGCTGACTGCGGCTCCAGCAGCAGAGCCGGCCGCCGATCCCGATGGGGCACTGCCGGATGT
CGGCCCGGTTCCAAACGACAAGCCGGCGATCCAATGA

Upstream 100 bases:

>100_bases
CCGTCGCCGCCGCCGCCTATCTCCGCCAGCCAAAACATCGATTCACCAAAATCACTTTTCACGATAGATTCGAACGCAAA
TCACTTCTCAAGGAAACAGC

Downstream 100 bases:

>100_bases
GAACGGTCGAGGCGGAGGGACAGTAGGCTGTCTTTCAAATCGCAAAGTCTTGGTATAGAAGCGGTCAAATCGATTGAATT
GCTTGGGCGGAGATTGGGTT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MAFGFAQAFRTFGKLTLAGAIGASLAAGDVGAEQKTPSPGSAKGQFGAVGLPTQGPAQPIGFYAKGCMTGAVALPADGPT
WEAMRLSRNRRWGNPAMISLLERLSQDGVKYAGWPGLLVGDIAQPRGGPMFNGHASHQIGLDADVWFTPMPARRMTAKER
EDLPFTTMLQKDKFLTVDPNVWTQSRARLLMLAASYPEVERIFVNPAIKKKMCDTWTGDRTNLGKLRPEYGHDSHFHIRI
KCPAGAAGCTPQAPVAAGDGCDKSLAWWFTPAPWAPPKPPKPGAKPPKPPREMMVSDLPKACAAVLDAASVASMQAATYG
GPSAASALTAAPAAEPAADPDGALPDVGPVPNDKPAIQ

Sequences:

>Translated_358_residues
MAFGFAQAFRTFGKLTLAGAIGASLAAGDVGAEQKTPSPGSAKGQFGAVGLPTQGPAQPIGFYAKGCMTGAVALPADGPT
WEAMRLSRNRRWGNPAMISLLERLSQDGVKYAGWPGLLVGDIAQPRGGPMFNGHASHQIGLDADVWFTPMPARRMTAKER
EDLPFTTMLQKDKFLTVDPNVWTQSRARLLMLAASYPEVERIFVNPAIKKKMCDTWTGDRTNLGKLRPEYGHDSHFHIRI
KCPAGAAGCTPQAPVAAGDGCDKSLAWWFTPAPWAPPKPPKPGAKPPKPPREMMVSDLPKACAAVLDAASVASMQAATYG
GPSAASALTAAPAAEPAADPDGALPDVGPVPNDKPAIQ
>Mature_357_residues
AFGFAQAFRTFGKLTLAGAIGASLAAGDVGAEQKTPSPGSAKGQFGAVGLPTQGPAQPIGFYAKGCMTGAVALPADGPTW
EAMRLSRNRRWGNPAMISLLERLSQDGVKYAGWPGLLVGDIAQPRGGPMFNGHASHQIGLDADVWFTPMPARRMTAKERE
DLPFTTMLQKDKFLTVDPNVWTQSRARLLMLAASYPEVERIFVNPAIKKKMCDTWTGDRTNLGKLRPEYGHDSHFHIRIK
CPAGAAGCTPQAPVAAGDGCDKSLAWWFTPAPWAPPKPPKPGAKPPKPPREMMVSDLPKACAAVLDAASVASMQAATYGG
PSAASALTAAPAAEPAADPDGALPDVGPVPNDKPAIQ

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=267, Percent_Identity=35.9550561797753, Blast_Score=160, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 37657; Mature: 37526

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFGFAQAFRTFGKLTLAGAIGASLAAGDVGAEQKTPSPGSAKGQFGAVGLPTQGPAQPI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCH
GFYAKGCMTGAVALPADGPTWEAMRLSRNRRWGNPAMISLLERLSQDGVKYAGWPGLLVG
HHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCCCEEEC
DIAQPRGGPMFNGHASHQIGLDADVWFTPMPARRMTAKEREDLPFTTMLQKDKFLTVDPN
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCHHHHHCCCCEEEECCC
VWTQSRARLLMLAASYPEVERIFVNPAIKKKMCDTWTGDRTNLGKLRPEYGHDSHFHIRI
CCHHCCCEEEEEECCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
KCPAGAAGCTPQAPVAAGDGCDKSLAWWFTPAPWAPPKPPKPGAKPPKPPREMMVSDLPK
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ACAAVLDAASVASMQAATYGGPSAASALTAAPAAEPAADPDGALPDVGPVPNDKPAIQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AFGFAQAFRTFGKLTLAGAIGASLAAGDVGAEQKTPSPGSAKGQFGAVGLPTQGPAQPI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCH
GFYAKGCMTGAVALPADGPTWEAMRLSRNRRWGNPAMISLLERLSQDGVKYAGWPGLLVG
HHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCCCEEEC
DIAQPRGGPMFNGHASHQIGLDADVWFTPMPARRMTAKEREDLPFTTMLQKDKFLTVDPN
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCHHHHHCCCCEEEECCC
VWTQSRARLLMLAASYPEVERIFVNPAIKKKMCDTWTGDRTNLGKLRPEYGHDSHFHIRI
CCHHCCCEEEEEECCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
KCPAGAAGCTPQAPVAAGDGCDKSLAWWFTPAPWAPPKPPKPGAKPPKPPREMMVSDLPK
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ACAAVLDAASVASMQAATYGGPSAASALTAAPAAEPAADPDGALPDVGPVPNDKPAIQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]