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The map label for this gene is potD [C]

Identifier: 209551679

GI number: 209551679

Start: 4289524

End: 4290573

Strand: Reverse

Name: potD [C]

Synonym: Rleg2_4108

Alternate gene names: 209551679

Gene position: 4290573-4289524 (Counterclockwise)

Preceding gene: 209551681

Following gene: 209551678

Centisome position: 94.55

GC content: 61.33

Gene sequence:

>1050_bases
ATGAAATTCAAGAACACTTCGACGATTACGGCTCTCGGCCTGCTATCGCTGGCGCTCGCCGCGGGACCGGGCGTGGCCCG
GGATCTTACCATAGCGTCCTGGGGCGGGAATTTTCAGGATGCGCAGCGGGAGATCTATTTCAAGCCGTTCTCCAATGTTA
CCGGCAAGCCTCTGCTCGACGAGGGCTGGGACGGCGGCATCGGCGTCCTCCAGGCAAAGGTCAAGGCGGGGAACCCTTCC
TGGGATGCCGTGGAGGTCGAAGCCGATGAACTCTCGCTCGGCTGCGCCGACGGACTTTTCGAGAAGGTGGACTGGAGCAA
GCTTGGCGGCAAGGATGCGTTTCTGCCTTCCGCGGTGAGCGATTGCGGCGTCGGCGCCATCGTCTGGTCGACCGCCATCG
CTTATGACGGCGCCAAGATCGCCGATGGCCCCAAATCCTGGGCCGAGTTCTGGGACGTGAAGAAATTCCCCGGCAAGCGC
GCCCTGCGCAAGGGACCGAAATATACGCTCGAATTCGCGCTGATGGCCGACGGCGTGCCGGCAGACAAGGTTTACGACGT
CCTGGCGACGCCTGAGGGCATCGATCGCGCCTTCGCCAAGCTTGATGCGTTGAAACCGAACCTTGTCTGGTGGACCTCGG
GCGCGCAGCCGTTGCAACTGCTGGCTTCCGGCGAAGTGGCCATGACCAGTGCCTATAACGGCCGCATCACCGGCATCAAC
CGGTCGGAAGGCCGGCAGTTCAAAGTTGTCTGGCCGGGCAGCATCTATGCCGTGGATAGCTGGGTCGTTCTGAAGGGCGC
TGAAAACAAGGATGCGGCCTTTGACTTCATCGCCTTCGCCAGCAAAGCGGAAAACCAGGCAAAACTGCCGCAATATATCG
CCTACGGCCTCCCGAACAAGGAAGCCGGCAAGAGCGTGCCGCCGGACCAGCTGGCCGACTTGCCCACCACCGAGGCCAAC
ATGCAGGAAGCAGTCAGCCTTGATACGGACTTCTGGATCGACAATTCGGAAGCGCTGACCAAGAGATTCGACGCCTGGCT
GGCACAATAG

Upstream 100 bases:

>100_bases
GCTTTGCCCATCCCTTGCGAGCGGCCGCGATTGCCAACGATCCCAAGGTTCGTGGCTTTCACCGGCGTCAGACGGACGCT
CAACAAACAGGGGAACGTGA

Downstream 100 bases:

>100_bases
CGGACGGAATATCGCCCCCGCGGGATCGCTTCTGCCGATCGACCCGTGGGGGCTTCCGCACATCTTCGTCACTTCGCTCC
CCAGGAGCCGACAATCCGAA

Product: family 1 extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKFKNTSTITALGLLSLALAAGPGVARDLTIASWGGNFQDAQREIYFKPFSNVTGKPLLDEGWDGGIGVLQAKVKAGNPS
WDAVEVEADELSLGCADGLFEKVDWSKLGGKDAFLPSAVSDCGVGAIVWSTAIAYDGAKIADGPKSWAEFWDVKKFPGKR
ALRKGPKYTLEFALMADGVPADKVYDVLATPEGIDRAFAKLDALKPNLVWWTSGAQPLQLLASGEVAMTSAYNGRITGIN
RSEGRQFKVVWPGSIYAVDSWVVLKGAENKDAAFDFIAFASKAENQAKLPQYIAYGLPNKEAGKSVPPDQLADLPTTEAN
MQEAVSLDTDFWIDNSEALTKRFDAWLAQ

Sequences:

>Translated_349_residues
MKFKNTSTITALGLLSLALAAGPGVARDLTIASWGGNFQDAQREIYFKPFSNVTGKPLLDEGWDGGIGVLQAKVKAGNPS
WDAVEVEADELSLGCADGLFEKVDWSKLGGKDAFLPSAVSDCGVGAIVWSTAIAYDGAKIADGPKSWAEFWDVKKFPGKR
ALRKGPKYTLEFALMADGVPADKVYDVLATPEGIDRAFAKLDALKPNLVWWTSGAQPLQLLASGEVAMTSAYNGRITGIN
RSEGRQFKVVWPGSIYAVDSWVVLKGAENKDAAFDFIAFASKAENQAKLPQYIAYGLPNKEAGKSVPPDQLADLPTTEAN
MQEAVSLDTDFWIDNSEALTKRFDAWLAQ
>Mature_349_residues
MKFKNTSTITALGLLSLALAAGPGVARDLTIASWGGNFQDAQREIYFKPFSNVTGKPLLDEGWDGGIGVLQAKVKAGNPS
WDAVEVEADELSLGCADGLFEKVDWSKLGGKDAFLPSAVSDCGVGAIVWSTAIAYDGAKIADGPKSWAEFWDVKKFPGKR
ALRKGPKYTLEFALMADGVPADKVYDVLATPEGIDRAFAKLDALKPNLVWWTSGAQPLQLLASGEVAMTSAYNGRITGIN
RSEGRQFKVVWPGSIYAVDSWVVLKGAENKDAAFDFIAFASKAENQAKLPQYIAYGLPNKEAGKSVPPDQLADLPTTEAN
MQEAVSLDTDFWIDNSEALTKRFDAWLAQ

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787367, Length=229, Percent_Identity=27.0742358078603, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37594; Mature: 37594

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKNTSTITALGLLSLALAAGPGVARDLTIASWGGNFQDAQREIYFKPFSNVTGKPLLD
CCCCCCCHHHHHHHHHHHHHCCCCCHHEEEEEECCCCHHCCCCEEEECCCCCCCCCCCCC
EGWDGGIGVLQAKVKAGNPSWDAVEVEADELSLGCADGLFEKVDWSKLGGKDAFLPSAVS
CCCCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCHHHHHHHHCCHHHCCCCCCCCCHHHH
DCGVGAIVWSTAIAYDGAKIADGPKSWAEFWDVKKFPGKRALRKGPKYTLEFALMADGVP
HCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEEECCCC
ADKVYDVLATPEGIDRAFAKLDALKPNLVWWTSGAQPLQLLASGEVAMTSAYNGRITGIN
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHEECCCEEEEECCCCEEEECC
RSEGRQFKVVWPGSIYAVDSWVVLKGAENKDAAFDFIAFASKAENQAKLPQYIAYGLPNK
CCCCCEEEEECCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCH
EAGKSVPPDQLADLPTTEANMQEAVSLDTDFWIDNSEALTKRFDAWLAQ
HCCCCCCHHHHHCCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFKNTSTITALGLLSLALAAGPGVARDLTIASWGGNFQDAQREIYFKPFSNVTGKPLLD
CCCCCCCHHHHHHHHHHHHHCCCCCHHEEEEEECCCCHHCCCCEEEECCCCCCCCCCCCC
EGWDGGIGVLQAKVKAGNPSWDAVEVEADELSLGCADGLFEKVDWSKLGGKDAFLPSAVS
CCCCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCHHHHHHHHCCHHHCCCCCCCCCHHHH
DCGVGAIVWSTAIAYDGAKIADGPKSWAEFWDVKKFPGKRALRKGPKYTLEFALMADGVP
HCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEEECCCC
ADKVYDVLATPEGIDRAFAKLDALKPNLVWWTSGAQPLQLLASGEVAMTSAYNGRITGIN
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHEECCCEEEEECCCCEEEECC
RSEGRQFKVVWPGSIYAVDSWVVLKGAENKDAAFDFIAFASKAENQAKLPQYIAYGLPNK
CCCCCEEEEECCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCH
EAGKSVPPDQLADLPTTEANMQEAVSLDTDFWIDNSEALTKRFDAWLAQ
HCCCCCCHHHHHCCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA