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The map label for this gene is dapE [H]

Identifier: 209551671

GI number: 209551671

Start: 4275965

End: 4277353

Strand: Reverse

Name: dapE [H]

Synonym: Rleg2_4100

Alternate gene names: 209551671

Gene position: 4277353-4275965 (Counterclockwise)

Preceding gene: 209551673

Following gene: 209551670

Centisome position: 94.26

GC content: 63.35

Gene sequence:

>1389_bases
ATGGCCGACATACAACCGGTTCTTTCGCGTGCGGACCAGAATCTTTCATCGAGCCTCGAAAAACTATTCGAGCTGCTGCG
CATCCAGTCGATTTCCACCGATCCCGCCTACAAAACCGAATGCCGCAAGGCGGCCGAATGGCTCGTCACCTATCTCGAAA
CGCTCGGCTTCGAAGCCTCCGTTCGCGACACGCCGGGCCATCCGATGGTCGTCGCCCACCATGCCGGCGCCTCCACTGAC
GCGCCCCACGTTCTGTTCTACGGCCATTACGACGTCCAGCCGGTCGACCCGATCGAGCTTTGGGATAACGACCCCTTCGA
GCCGTCGATCAAGGATGTCGGCGACGGCCGCAAGATCCTGACCGGCCGCGGCACCTCCGACGACAAGGGCCAGCTGATGA
CCTTCGTCGAGGCCTGCCGCGCCTATAAGGAGATCAACGGCGCGCTTCCCTGCCGCGTCACCATTCTCTTCGAAGGCGAG
GAAGAATCCGGCTCGCCATCGCTGAAGCCCTTCCTCGAAGCCAATGCCGCCGAGCTCAAGGCCGATTATGCGCTGGTCTG
CGATACCGGCATGTGGGACCGCGACACGCCGGCGATCGCCGCGGCATTGCGCGGCCTTGTCGGCGAGGAAGTCACCGTGA
CGGCCGCCGACCGCGACCTGCATTCCGGCCTCTTCGGGGGCGCTGCCGCCAATCCGATCCACGTCCTCGTCGAGGCTCTT
GCCGGCCTGCATGACGAGACCGGCCGCATCACGCTCGACGGCTTCTACAACGGCGTCGAGGAGACGCCCGACAACATCAA
GGCCTCATGGGAGACGCTGGGCAAGACCGCCGAGACCTTCCTCGGCGAAGTCGGCCTTTCCATTCCATCCGGCGAAAAGG
GCCGTTCGGTACTGGAACTCACCTGGGCGCGGCCGACCGCCGAAATCAACGGCATCTGGGGCGGATATACCGGCGAAGGC
TTCAAGACGGTGATCGCCGCCAAGGCTTCGGCGAAGGTTTCATTCCGGCTTGTCGGCACGCAGAATCCGGCCGCCATCCG
CGAAGCCTTCCGCAGCTATATCAGTTCGAAGATTCCCGCCGATTGCTCGGTCGAGTTCCATCCGCATGGCGGCTCGCCGG
CGATCCACCTCTCCTATGATTCGCCGGTTCTGACCAAGGCGAAGACCGCGCTGTCCGACGAGTGGCCGAAACCCGCCATC
GTCATCGGCATGGGTGGCTCGATCCCGATCGTCGGCGACTTCCAGAAAATGCTCGGCATGGAATCGCTGCTCGTCGGCTT
CGGCCTCAGCGATGACCGCATCCATTCGCCGAACGAGAAATACGAGCTCGTCTCCTACCACAAGGGCATCCGCTCTTGGG
TGCGGATTCTTCAGGCGCTCGCCGCCTGA

Upstream 100 bases:

>100_bases
TCGGCATGATTGCGCGCGCCTCTGATTTTTTGCTTTTTCCGCCTCGCCTGCTTTCCCGGTTTGGTCTATCGTCCCGCCGA
ATTTCGAAAAGGAGTCGAAA

Downstream 100 bases:

>100_bases
ACGAAAGGGTGTGCCAGATCGCAATAACAAAAAGGCCGGTTAAAACCGGCCTTTCGTCATCCGCTCGCTCAGTGCCAGTT
CATCCGTGGTCAATGAGAAG

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MADIQPVLSRADQNLSSSLEKLFELLRIQSISTDPAYKTECRKAAEWLVTYLETLGFEASVRDTPGHPMVVAHHAGASTD
APHVLFYGHYDVQPVDPIELWDNDPFEPSIKDVGDGRKILTGRGTSDDKGQLMTFVEACRAYKEINGALPCRVTILFEGE
EESGSPSLKPFLEANAAELKADYALVCDTGMWDRDTPAIAAALRGLVGEEVTVTAADRDLHSGLFGGAAANPIHVLVEAL
AGLHDETGRITLDGFYNGVEETPDNIKASWETLGKTAETFLGEVGLSIPSGEKGRSVLELTWARPTAEINGIWGGYTGEG
FKTVIAAKASAKVSFRLVGTQNPAAIREAFRSYISSKIPADCSVEFHPHGGSPAIHLSYDSPVLTKAKTALSDEWPKPAI
VIGMGGSIPIVGDFQKMLGMESLLVGFGLSDDRIHSPNEKYELVSYHKGIRSWVRILQALAA

Sequences:

>Translated_462_residues
MADIQPVLSRADQNLSSSLEKLFELLRIQSISTDPAYKTECRKAAEWLVTYLETLGFEASVRDTPGHPMVVAHHAGASTD
APHVLFYGHYDVQPVDPIELWDNDPFEPSIKDVGDGRKILTGRGTSDDKGQLMTFVEACRAYKEINGALPCRVTILFEGE
EESGSPSLKPFLEANAAELKADYALVCDTGMWDRDTPAIAAALRGLVGEEVTVTAADRDLHSGLFGGAAANPIHVLVEAL
AGLHDETGRITLDGFYNGVEETPDNIKASWETLGKTAETFLGEVGLSIPSGEKGRSVLELTWARPTAEINGIWGGYTGEG
FKTVIAAKASAKVSFRLVGTQNPAAIREAFRSYISSKIPADCSVEFHPHGGSPAIHLSYDSPVLTKAKTALSDEWPKPAI
VIGMGGSIPIVGDFQKMLGMESLLVGFGLSDDRIHSPNEKYELVSYHKGIRSWVRILQALAA
>Mature_461_residues
ADIQPVLSRADQNLSSSLEKLFELLRIQSISTDPAYKTECRKAAEWLVTYLETLGFEASVRDTPGHPMVVAHHAGASTDA
PHVLFYGHYDVQPVDPIELWDNDPFEPSIKDVGDGRKILTGRGTSDDKGQLMTFVEACRAYKEINGALPCRVTILFEGEE
ESGSPSLKPFLEANAAELKADYALVCDTGMWDRDTPAIAAALRGLVGEEVTVTAADRDLHSGLFGGAAANPIHVLVEALA
GLHDETGRITLDGFYNGVEETPDNIKASWETLGKTAETFLGEVGLSIPSGEKGRSVLELTWARPTAEINGIWGGYTGEGF
KTVIAAKASAKVSFRLVGTQNPAAIREAFRSYISSKIPADCSVEFHPHGGSPAIHLSYDSPVLTKAKTALSDEWPKPAIV
IGMGGSIPIVGDFQKMLGMESLLVGFGLSDDRIHSPNEKYELVSYHKGIRSWVRILQALAA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=468, Percent_Identity=28.4188034188034, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI21071039, Length=483, Percent_Identity=27.1221532091097, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI271398277, Length=310, Percent_Identity=28.7096774193548, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17563146, Length=476, Percent_Identity=30.4621848739496, Blast_Score=195, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI193211059, Length=479, Percent_Identity=28.6012526096033, Blast_Score=181, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6321159, Length=471, Percent_Identity=27.8131634819533, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6319758, Length=480, Percent_Identity=24.375, Blast_Score=119, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24585880, Length=469, Percent_Identity=31.1300639658849, Blast_Score=191, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 49865; Mature: 49734

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIQPVLSRADQNLSSSLEKLFELLRIQSISTDPAYKTECRKAAEWLVTYLETLGFEAS
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VRDTPGHPMVVAHHAGASTDAPHVLFYGHYDVQPVDPIELWDNDPFEPSIKDVGDGRKIL
CCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCHHHCCCCCEEE
TGRGTSDDKGQLMTFVEACRAYKEINGALPCRVTILFEGEEESGSPSLKPFLEANAAELK
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHCCHHHHC
ADYALVCDTGMWDRDTPAIAAALRGLVGEEVTVTAADRDLHSGLFGGAAANPIHVLVEAL
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCHHHHHHHHH
AGLHDETGRITLDGFYNGVEETPDNIKASWETLGKTAETFLGEVGLSIPSGEKGRSVLEL
HCCCCCCCEEEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
TWARPTAEINGIWGGYTGEGFKTVIAAKASAKVSFRLVGTQNPAAIREAFRSYISSKIPA
EECCCCCEECCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCC
DCSVEFHPHGGSPAIHLSYDSPVLTKAKTALSDEWPKPAIVIGMGGSIPIVGDFQKMLGM
CCEEEEECCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEECCCCCCEEECHHHHHCH
ESLLVGFGLSDDRIHSPNEKYELVSYHKGIRSWVRILQALAA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADIQPVLSRADQNLSSSLEKLFELLRIQSISTDPAYKTECRKAAEWLVTYLETLGFEAS
CCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VRDTPGHPMVVAHHAGASTDAPHVLFYGHYDVQPVDPIELWDNDPFEPSIKDVGDGRKIL
CCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCHHHCCCCCEEE
TGRGTSDDKGQLMTFVEACRAYKEINGALPCRVTILFEGEEESGSPSLKPFLEANAAELK
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHCCHHHHC
ADYALVCDTGMWDRDTPAIAAALRGLVGEEVTVTAADRDLHSGLFGGAAANPIHVLVEAL
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCHHHHHHHHH
AGLHDETGRITLDGFYNGVEETPDNIKASWETLGKTAETFLGEVGLSIPSGEKGRSVLEL
HCCCCCCCEEEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
TWARPTAEINGIWGGYTGEGFKTVIAAKASAKVSFRLVGTQNPAAIREAFRSYISSKIPA
EECCCCCEECCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCC
DCSVEFHPHGGSPAIHLSYDSPVLTKAKTALSDEWPKPAIVIGMGGSIPIVGDFQKMLGM
CCEEEEECCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEECCCCCCEEECHHHHHCH
ESLLVGFGLSDDRIHSPNEKYELVSYHKGIRSWVRILQALAA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA