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The map label for this gene is mltB [H]

Identifier: 209551644

GI number: 209551644

Start: 4249398

End: 4250669

Strand: Reverse

Name: mltB [H]

Synonym: Rleg2_4073

Alternate gene names: 209551644

Gene position: 4250669-4249398 (Counterclockwise)

Preceding gene: 209551645

Following gene: 209551641

Centisome position: 93.67

GC content: 63.13

Gene sequence:

>1272_bases
ATGTCCGGCAGGACAGAGGGGGGTGTTCCACGACGGCTGCGGATATTCGCATTGGCGATAATGACCGCCCTCCTCCCCCT
CTCCGCACACGCAGCCCCTTCAAAAACCGATGTCGAGGCGCAGTTCGAGACGTGGGTGCAGAAGGACCTCTGGCCGGAGG
CGAAGGCGAACGGGATTTCCGAGAAGAGCTTCAAGGCCGCTTTCTCCGGCATCACCCTGAACTGGAACCTGCCCGATCTC
GCCCCGCCCGGTTTCCCGCCGCCGAAGGAGCAGAAGCAGACGCAGGCGGAGTTTTCCTCGCCCGGTCCCTATTTCAACGA
GGACCATCTGAAGAGGCTTGCCGCAACCGGCCGCGGCTTTGCCGCGCAATATGGCTCGACGCTGAAGCGGATCGAGAAGA
CCTATGGTGTGCCGGGGCCGATCGTGCTGGCCATCTGGGGTCGGGAGACCGGCTTCGGCGCCGCGAAGATCCCGAATTCG
GCGATCGAGGTGCTGGCGACCAAGGCCTTCATGTCGACGCGCAAGGAGATGTTCCGCACCGAACTGGTGGCGGCGCTGCA
TATTCTCGACGGCGGCGACGTGACGCCTGCCGATTTCAAGGGTTCGTGGGCCGGCGCGCTCGGGCAGCCGCAATTCATGC
CGACGAGCTACCTGAAATATGCCGTCGATTTCGACGGCGACGGCCATCGCAATATCTGGACCTCGGTGCCGGATACGCTC
GCCTCGATCGCCAATTTCCTGGTCAAGAAGGGCTGGCAGCGTGACCGCGACTGGGGCTTCGAGGTGTCGATACCAGAGGC
GGTCTCCTGCGCGCAGGAAGGCCCGGATCTCGCAAAGCCGCTGTCACACTGGGCCTCGCTCGGCATCGACCGCATCTCCG
GCAAGGATTTCCCCTCAAGCGAGATGAAGGCCGGGGGCATGATGCTGGTTCCGGCGGGCCGCGACGGGCCTGAGTTCATC
GTCACGCCGAATTTCTACATCATCAAGGAATACAATAATTCCGATCTCTACGCGCTCTATATCGGCAATCTCGCCGACCG
GATCGGCTCGAATGGCGGCGCCTTTCAGGGTCGCTGGGGCGATGTCGGCAAGATGCTGCGCTCGGATGTCGCCGCCATGC
AGAAGGCGCTGGAACGGCAGGGCTACGATGTCGGCGGTTCCGACGGCCTGCCGGGCTACAAGACCCGGCGTTCGATCGGC
CAGTGGCAGGCGAAGAACGGCATGAAGCCGACCTGCTTTCCCGAAGCCACGATGAAAGGCAAGCTCAAGTAG

Upstream 100 bases:

>100_bases
GGCGTCGCGAAATTTTGACCGCGCGCCAGGCGGCGGCGATCTGCAGCGTTTGGTTCGGACGAGAGGGTGCCCCCAGCCGA
TCTCCCCCCTTGTGGGGGAG

Downstream 100 bases:

>100_bases
CGTTCAGAGATTGCGCTTCAGCCCTTCGTGGCTCGCGACGAAGCCGAGCCTCTCGTAGAAGCGGATCGAATCCGCCCGCG
TCTTGTCCGATGTCAGCTGC

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSGRTEGGVPRRLRIFALAIMTALLPLSAHAAPSKTDVEAQFETWVQKDLWPEAKANGISEKSFKAAFSGITLNWNLPDL
APPGFPPPKEQKQTQAEFSSPGPYFNEDHLKRLAATGRGFAAQYGSTLKRIEKTYGVPGPIVLAIWGRETGFGAAKIPNS
AIEVLATKAFMSTRKEMFRTELVAALHILDGGDVTPADFKGSWAGALGQPQFMPTSYLKYAVDFDGDGHRNIWTSVPDTL
ASIANFLVKKGWQRDRDWGFEVSIPEAVSCAQEGPDLAKPLSHWASLGIDRISGKDFPSSEMKAGGMMLVPAGRDGPEFI
VTPNFYIIKEYNNSDLYALYIGNLADRIGSNGGAFQGRWGDVGKMLRSDVAAMQKALERQGYDVGGSDGLPGYKTRRSIG
QWQAKNGMKPTCFPEATMKGKLK

Sequences:

>Translated_423_residues
MSGRTEGGVPRRLRIFALAIMTALLPLSAHAAPSKTDVEAQFETWVQKDLWPEAKANGISEKSFKAAFSGITLNWNLPDL
APPGFPPPKEQKQTQAEFSSPGPYFNEDHLKRLAATGRGFAAQYGSTLKRIEKTYGVPGPIVLAIWGRETGFGAAKIPNS
AIEVLATKAFMSTRKEMFRTELVAALHILDGGDVTPADFKGSWAGALGQPQFMPTSYLKYAVDFDGDGHRNIWTSVPDTL
ASIANFLVKKGWQRDRDWGFEVSIPEAVSCAQEGPDLAKPLSHWASLGIDRISGKDFPSSEMKAGGMMLVPAGRDGPEFI
VTPNFYIIKEYNNSDLYALYIGNLADRIGSNGGAFQGRWGDVGKMLRSDVAAMQKALERQGYDVGGSDGLPGYKTRRSIG
QWQAKNGMKPTCFPEATMKGKLK
>Mature_422_residues
SGRTEGGVPRRLRIFALAIMTALLPLSAHAAPSKTDVEAQFETWVQKDLWPEAKANGISEKSFKAAFSGITLNWNLPDLA
PPGFPPPKEQKQTQAEFSSPGPYFNEDHLKRLAATGRGFAAQYGSTLKRIEKTYGVPGPIVLAIWGRETGFGAAKIPNSA
IEVLATKAFMSTRKEMFRTELVAALHILDGGDVTPADFKGSWAGALGQPQFMPTSYLKYAVDFDGDGHRNIWTSVPDTLA
SIANFLVKKGWQRDRDWGFEVSIPEAVSCAQEGPDLAKPLSHWASLGIDRISGKDFPSSEMKAGGMMLVPAGRDGPEFIV
TPNFYIIKEYNNSDLYALYIGNLADRIGSNGGAFQGRWGDVGKMLRSDVAAMQKALERQGYDVGGSDGLPGYKTRRSIGQ
WQAKNGMKPTCFPEATMKGKLK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=238, Percent_Identity=33.1932773109244, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 46075; Mature: 45944

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRTEGGVPRRLRIFALAIMTALLPLSAHAAPSKTDVEAQFETWVQKDLWPEAKANGIS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCC
EKSFKAAFSGITLNWNLPDLAPPGFPPPKEQKQTQAEFSSPGPYFNEDHLKRLAATGRGF
HHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCH
AAQYGSTLKRIEKTYGVPGPIVLAIWGRETGFGAAKIPNSAIEVLATKAFMSTRKEMFRT
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
ELVAALHILDGGDVTPADFKGSWAGALGQPQFMPTSYLKYAVDFDGDGHRNIWTSVPDTL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCHHHH
ASIANFLVKKGWQRDRDWGFEVSIPEAVSCAQEGPDLAKPLSHWASLGIDRISGKDFPSS
HHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHCCCHHHHHHHHHHHCCCHHCCCCCCCCH
EMKAGGMMLVPAGRDGPEFIVTPNFYIIKEYNNSDLYALYIGNLADRIGSNGGAFQGRWG
HHCCCCEEEEECCCCCCCEEECCCEEEEEEECCCCEEEEEECHHHHHHCCCCCCCCCCHH
DVGKMLRSDVAAMQKALERQGYDVGGSDGLPGYKTRRSIGQWQAKNGMKPTCFPEATMKG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KLK
CCC
>Mature Secondary Structure 
SGRTEGGVPRRLRIFALAIMTALLPLSAHAAPSKTDVEAQFETWVQKDLWPEAKANGIS
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCC
EKSFKAAFSGITLNWNLPDLAPPGFPPPKEQKQTQAEFSSPGPYFNEDHLKRLAATGRGF
HHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCH
AAQYGSTLKRIEKTYGVPGPIVLAIWGRETGFGAAKIPNSAIEVLATKAFMSTRKEMFRT
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
ELVAALHILDGGDVTPADFKGSWAGALGQPQFMPTSYLKYAVDFDGDGHRNIWTSVPDTL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCHHHH
ASIANFLVKKGWQRDRDWGFEVSIPEAVSCAQEGPDLAKPLSHWASLGIDRISGKDFPSS
HHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHCCCHHHHHHHHHHHCCCHHCCCCCCCCH
EMKAGGMMLVPAGRDGPEFIVTPNFYIIKEYNNSDLYALYIGNLADRIGSNGGAFQGRWG
HHCCCCEEEEECCCCCCCEEECCCEEEEEEECCCCEEEEEECHHHHHHCCCCCCCCCCHH
DVGKMLRSDVAAMQKALERQGYDVGGSDGLPGYKTRRSIGQWQAKNGMKPTCFPEATMKG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KLK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]