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The map label for this gene is puuE [H]

Identifier: 209551597

GI number: 209551597

Start: 4199044

End: 4200324

Strand: Reverse

Name: puuE [H]

Synonym: Rleg2_4026

Alternate gene names: 209551597

Gene position: 4200324-4199044 (Counterclockwise)

Preceding gene: 209551601

Following gene: 209551596

Centisome position: 92.56

GC content: 64.32

Gene sequence:

>1281_bases
ATGACCGCGACGAGCCTTACGGACCGCAAGAACGCCGCCATTTCACGGGGTGTCGGCATGACGACCCAGATCTATGCCGA
TCGCGCCGAGAACGCGGAGATCTGGGACAAGGAGGGCCGCCGCTACATCGATTTCGCCGCCGGCATCGCCGTTCTCAACA
CCGGCCACCGCCATCCCAGGGTGATTGCGGCGGTCAAGGATCAGCTCGACCACTTTACCCATACCTGCCATCAGGTGGTG
CCCTATGAGAACTATGTCAGTCTCGCCGAGCGGCTGAATGCACTGGTGCCAGGTGACTTCGAGAAGAAGACGATCTTCGT
CACCACGGGTGCCGAAGCGGTCGAAAATGCCGTCAAGATTGCGCGGGCGGCAACCGGCCGCTCGGCTATCATCGCCTTTG
GCGGCGGTTTCCATGGCCGTACCTTCATGGGCATGGCGCTGACCGGCAAGGTGGTGCCCTACAAGGTCGGTTTCGGCGCG
ATGCCGGGCGATGTCTTCCATATTCCCTTCCCGGTCGAGCTGCACGGCGTTACCGCCGATCAGTCGCTGGCGGCGCTGAG
GAAGCTCTTTGCCGCCGATGTCGATCCGCAGCGGGTCGCCGCCATCATTATCGAGGCCGTGCAGGGCGAAGGCGGTTTCT
ACCCGGCGCCCGCGGCCTTCATGAAAGCGCTGCGCGAACTCTGCGACCAGCACGGCATCCTGCTGATCGCCGACGAGGTG
CAGACCGGTTTTGCCCGCACCGGCAAGATGTTCGCGATGGATCATCACGAGGTGGCGGCCGACCTGACGACGATGGCAAA
GAGCCTTGCCGGCGGCTTTCCGCTCGCCGCCGTCACCGGCCGCGCCGACATCATGGACGCGCCGGGACCGGGCGGGCTCG
GCGGCACTTATGGCGGCAATCCAATCGGAATCGCCGCAGCGCATGCCGTCCTCGACGTCATCGCCGACGAGGATCTCTGC
AACCGCGCCAATCTGCTCGGCGGGCGGCTGAAGCAGCGGCTGGAATCGCTGCGCGAGACGGTGCCTGAGATCTCCGATAT
CCGTGGGCCGGGTTTCATGAATGCCGTCGAATTCAACGACCGGACGACGGGCTTGCCGAGCGCCGAATTCGCCAATCGAG
TACGGCTGACAGCGCTCGACAAGGGCCTGATCCTGCTCACCTGCGGCGTCCACGGCAACGTCATCCGCTTCCTTGCACCG
ATCACCATCCAGGACGAGGTGCTCGGGGAGGCGCTCGACATTCTCGAGGCCTCGATGCTGGAAGTGAGTGCGGCCAAGTG
A

Upstream 100 bases:

>100_bases
ATCCCACTTGTTTTCTTGCAGGAAAAATGTAGGAGTTTTATAAATAACTTGCAGAACTTATACAAGATAATTTCAACCCC
CTTCAGACAGGAGCGCATCG

Downstream 100 bases:

>100_bases
GGCGACAGGCACCGGATTTCAATGGCTGCCGGCGACCGGCAGCCGCTGCCAAGGAGATATGACATGGCTTTCACCACCGC
GCTGACCAGACACGTTCCCT

Product: 4-aminobutyrate aminotransferase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTATSLTDRKNAAISRGVGMTTQIYADRAENAEIWDKEGRRYIDFAAGIAVLNTGHRHPRVIAAVKDQLDHFTHTCHQVV
PYENYVSLAERLNALVPGDFEKKTIFVTTGAEAVENAVKIARAATGRSAIIAFGGGFHGRTFMGMALTGKVVPYKVGFGA
MPGDVFHIPFPVELHGVTADQSLAALRKLFAADVDPQRVAAIIIEAVQGEGGFYPAPAAFMKALRELCDQHGILLIADEV
QTGFARTGKMFAMDHHEVAADLTTMAKSLAGGFPLAAVTGRADIMDAPGPGGLGGTYGGNPIGIAAAHAVLDVIADEDLC
NRANLLGGRLKQRLESLRETVPEISDIRGPGFMNAVEFNDRTTGLPSAEFANRVRLTALDKGLILLTCGVHGNVIRFLAP
ITIQDEVLGEALDILEASMLEVSAAK

Sequences:

>Translated_426_residues
MTATSLTDRKNAAISRGVGMTTQIYADRAENAEIWDKEGRRYIDFAAGIAVLNTGHRHPRVIAAVKDQLDHFTHTCHQVV
PYENYVSLAERLNALVPGDFEKKTIFVTTGAEAVENAVKIARAATGRSAIIAFGGGFHGRTFMGMALTGKVVPYKVGFGA
MPGDVFHIPFPVELHGVTADQSLAALRKLFAADVDPQRVAAIIIEAVQGEGGFYPAPAAFMKALRELCDQHGILLIADEV
QTGFARTGKMFAMDHHEVAADLTTMAKSLAGGFPLAAVTGRADIMDAPGPGGLGGTYGGNPIGIAAAHAVLDVIADEDLC
NRANLLGGRLKQRLESLRETVPEISDIRGPGFMNAVEFNDRTTGLPSAEFANRVRLTALDKGLILLTCGVHGNVIRFLAP
ITIQDEVLGEALDILEASMLEVSAAK
>Mature_425_residues
TATSLTDRKNAAISRGVGMTTQIYADRAENAEIWDKEGRRYIDFAAGIAVLNTGHRHPRVIAAVKDQLDHFTHTCHQVVP
YENYVSLAERLNALVPGDFEKKTIFVTTGAEAVENAVKIARAATGRSAIIAFGGGFHGRTFMGMALTGKVVPYKVGFGAM
PGDVFHIPFPVELHGVTADQSLAALRKLFAADVDPQRVAAIIIEAVQGEGGFYPAPAAFMKALRELCDQHGILLIADEVQ
TGFARTGKMFAMDHHEVAADLTTMAKSLAGGFPLAAVTGRADIMDAPGPGGLGGTYGGNPIGIAAAHAVLDVIADEDLCN
RANLLGGRLKQRLESLRETVPEISDIRGPGFMNAVEFNDRTTGLPSAEFANRVRLTALDKGLILLTCGVHGNVIRFLAPI
TIQDEVLGEALDILEASMLEVSAAK

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=410, Percent_Identity=33.9024390243902, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI284507298, Length=325, Percent_Identity=35.3846153846154, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI13994255, Length=417, Percent_Identity=30.9352517985612, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI37574042, Length=417, Percent_Identity=29.4964028776978, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI226442705, Length=417, Percent_Identity=29.2565947242206, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI226442709, Length=390, Percent_Identity=29.7435897435897, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI24119277, Length=395, Percent_Identity=27.8481012658228, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI188536080, Length=413, Percent_Identity=26.3922518159806, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI38679950, Length=413, Percent_Identity=26.3922518159806, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI38679946, Length=413, Percent_Identity=26.3922518159806, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1787560, Length=419, Percent_Identity=60.381861575179, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1789016, Length=418, Percent_Identity=56.2200956937799, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1789759, Length=404, Percent_Identity=33.6633663366337, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1788044, Length=397, Percent_Identity=34.5088161209068, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI145693181, Length=384, Percent_Identity=33.8541666666667, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786349, Length=329, Percent_Identity=34.0425531914894, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1786991, Length=343, Percent_Identity=31.4868804664723, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25144271, Length=408, Percent_Identity=29.1666666666667, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI32564660, Length=425, Percent_Identity=29.6470588235294, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71992977, Length=435, Percent_Identity=30.8045977011494, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25144274, Length=220, Percent_Identity=34.0909090909091, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17541228, Length=426, Percent_Identity=23.0046948356808, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323470, Length=408, Percent_Identity=32.843137254902, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6324432, Length=410, Percent_Identity=30.7317073170732, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6321456, Length=309, Percent_Identity=26.2135922330097, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324386, Length=314, Percent_Identity=23.2484076433121, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357415, Length=411, Percent_Identity=32.8467153284672, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574759, Length=416, Percent_Identity=31.4903846153846, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI161085790, Length=411, Percent_Identity=31.6301703163017, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI21356575, Length=418, Percent_Identity=30.8612440191388, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI281366494, Length=382, Percent_Identity=25.9162303664921, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667139, Length=382, Percent_Identity=25.9162303664921, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667143, Length=382, Percent_Identity=25.9162303664921, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 45325; Mature: 45193

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATSLTDRKNAAISRGVGMTTQIYADRAENAEIWDKEGRRYIDFAAGIAVLNTGHRHPR
CCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCHHHHCCCEEEEHHHCHHHCCCCCCCCH
VIAAVKDQLDHFTHTCHQVVPYENYVSLAERLNALVPGDFEKKTIFVTTGAEAVENAVKI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHH
ARAATGRSAIIAFGGGFHGRTFMGMALTGKVVPYKVGFGAMPGDVFHIPFPVELHGVTAD
HHHCCCCEEEEEECCCCCCCEEEEEEEECCEEEEEECCCCCCCCEEECCCCEEEECCCCC
QSLAALRKLFAADVDPQRVAAIIIEAVQGEGGFYPAPAAFMKALRELCDQHGILLIADEV
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCH
QTGFARTGKMFAMDHHEVAADLTTMAKSLAGGFPLAAVTGRADIMDAPGPGGLGGTYGGN
HHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
PIGIAAAHAVLDVIADEDLCNRANLLGGRLKQRLESLRETVPEISDIRGPGFMNAVEFND
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
RTTGLPSAEFANRVRLTALDKGLILLTCGVHGNVIRFLAPITIQDEVLGEALDILEASML
CCCCCCCHHHHCCEEEEEECCCEEEEEECCCCCEEHHHCCCCCCHHHHHHHHHHHHHHHH
EVSAAK
HHCCCC
>Mature Secondary Structure 
TATSLTDRKNAAISRGVGMTTQIYADRAENAEIWDKEGRRYIDFAAGIAVLNTGHRHPR
CCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCHHHHCCCEEEEHHHCHHHCCCCCCCCH
VIAAVKDQLDHFTHTCHQVVPYENYVSLAERLNALVPGDFEKKTIFVTTGAEAVENAVKI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHH
ARAATGRSAIIAFGGGFHGRTFMGMALTGKVVPYKVGFGAMPGDVFHIPFPVELHGVTAD
HHHCCCCEEEEEECCCCCCCEEEEEEEECCEEEEEECCCCCCCCEEECCCCEEEECCCCC
QSLAALRKLFAADVDPQRVAAIIIEAVQGEGGFYPAPAAFMKALRELCDQHGILLIADEV
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCH
QTGFARTGKMFAMDHHEVAADLTTMAKSLAGGFPLAAVTGRADIMDAPGPGGLGGTYGGN
HHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
PIGIAAAHAVLDVIADEDLCNRANLLGGRLKQRLESLRETVPEISDIRGPGFMNAVEFND
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
RTTGLPSAEFANRVRLTALDKGLILLTCGVHGNVIRFLAPITIQDEVLGEALDILEASML
CCCCCCCHHHHCCEEEEEECCCEEEEEECCCCCEEHHHCCCCCCHHHHHHHHHHHHHHHH
EVSAAK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]