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The map label for this gene is purR [H]

Identifier: 209551578

GI number: 209551578

Start: 4175482

End: 4176513

Strand: Direct

Name: purR [H]

Synonym: Rleg2_4007

Alternate gene names: 209551578

Gene position: 4175482-4176513 (Clockwise)

Preceding gene: 209551576

Following gene: 209551579

Centisome position: 92.01

GC content: 65.21

Gene sequence:

>1032_bases
ATGAACGAACAGAAAATCAGACGGCCGCGCCAGGCCGATATCGCCACATTGGCCGGCGTCTCCGTCTCGACGGTGTCGCG
GGTGCTCGCCAACGAACCCGGCATCAGCGAAACCGTGCGCCGCCACATCTTGAAGGTGGCTGCCGAGCACGGCTATCCCG
TCAAGTCTGCAACCGAAGCTGTTGCCGGCGGGCTGGCGCTGATCGCCAGCGATGGCGTCACCGGCGGCCTCAGCGTCTTT
TACGAAGCGATCGTCGACGGCTTGCGCGCCGGCGCTGCCGAAGCGGGCATGCCTTTCGAAATCCGCATGGTCCGCGAGGA
TCGCACAACGCCGGATGCCGTGCGCGACTATATGCGGGCAGCGAGCGCCGAAGGGCTCTTTCTGGTCGGCATCGATCCGA
ACGAGATGCTGCGCGCCTGGCTGCTGGATAGCATGACGCCGGCGGTGCTCGTCAATGGTACCGATCCGATGATGCAGTTG
GACGGCGTTTCGCCGGCTAATTTCTTCGGCGCCTATCAGGCGACCAGCCGGCTGATGAAAGCCGGCCACCGCCGCATCCT
GCATCTGAGCGGGTCTCACCGCCATACGATCCGAGAGCGTATCCGCGGTTTCGAGGCGGCGATCGCCGCCGTTCCCGGCG
CCGAGGGCCGTTTCGTCGCCCTCGCGTTGCAGGGCAGCGCCAGCGGCGAGGCCCATGAGCGCACGGCTGCGATCCTTGCT
GAAAATGCCGGTTTCACCGCCGCCTTCTGCATGAATGATTTCATCGCCGTCGGCGTGCTGGAGGCCGTCACCGAAGCCGG
TCTGCGTGTGCCGGAGGATTTCGCCATCGTCGGCTTCGATGATCTGCCCTGCGCGCTGATGACAAATCCGCAGCTTTCCA
CCATGCGCGTCGACCGTGCCGCCCTCGGGCGTGAGGCCATCTCGCTGATGCTGTCCCGTTTCCGCAACAGGACGGCGCCG
GCGCGCCACATTTGCCAGGCTGTCATTCCCGTTCCGGGAGGGACCGTTCCGAACGCTGAAACCCGTGAGTGA

Upstream 100 bases:

>100_bases
GCGTTGCGAGCAATTCGGCACAAAGAGTACGCAAAAAAATAATTATTGCAAGAAATAATGATTATCTTGCAAATTTGCAT
AACTTGCATAGACTTTGGTT

Downstream 100 bases:

>100_bases
TGCCGATGACCTATGATCCCGCCAGCGCCAACCCGCTCGCAGGCAATCCGCTGGAAACCCGCGCCGACATGAGCCGGGCC
CTGCTTGAACTCTTCGATCC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MNEQKIRRPRQADIATLAGVSVSTVSRVLANEPGISETVRRHILKVAAEHGYPVKSATEAVAGGLALIASDGVTGGLSVF
YEAIVDGLRAGAAEAGMPFEIRMVREDRTTPDAVRDYMRAASAEGLFLVGIDPNEMLRAWLLDSMTPAVLVNGTDPMMQL
DGVSPANFFGAYQATSRLMKAGHRRILHLSGSHRHTIRERIRGFEAAIAAVPGAEGRFVALALQGSASGEAHERTAAILA
ENAGFTAAFCMNDFIAVGVLEAVTEAGLRVPEDFAIVGFDDLPCALMTNPQLSTMRVDRAALGREAISLMLSRFRNRTAP
ARHICQAVIPVPGGTVPNAETRE

Sequences:

>Translated_343_residues
MNEQKIRRPRQADIATLAGVSVSTVSRVLANEPGISETVRRHILKVAAEHGYPVKSATEAVAGGLALIASDGVTGGLSVF
YEAIVDGLRAGAAEAGMPFEIRMVREDRTTPDAVRDYMRAASAEGLFLVGIDPNEMLRAWLLDSMTPAVLVNGTDPMMQL
DGVSPANFFGAYQATSRLMKAGHRRILHLSGSHRHTIRERIRGFEAAIAAVPGAEGRFVALALQGSASGEAHERTAAILA
ENAGFTAAFCMNDFIAVGVLEAVTEAGLRVPEDFAIVGFDDLPCALMTNPQLSTMRVDRAALGREAISLMLSRFRNRTAP
ARHICQAVIPVPGGTVPNAETRE
>Mature_343_residues
MNEQKIRRPRQADIATLAGVSVSTVSRVLANEPGISETVRRHILKVAAEHGYPVKSATEAVAGGLALIASDGVTGGLSVF
YEAIVDGLRAGAAEAGMPFEIRMVREDRTTPDAVRDYMRAASAEGLFLVGIDPNEMLRAWLLDSMTPAVLVNGTDPMMQL
DGVSPANFFGAYQATSRLMKAGHRRILHLSGSHRHTIRERIRGFEAAIAAVPGAEGRFVALALQGSASGEAHERTAAILA
ENAGFTAAFCMNDFIAVGVLEAVTEAGLRVPEDFAIVGFDDLPCALMTNPQLSTMRVDRAALGREAISLMLSRFRNRTAP
ARHICQAVIPVPGGTVPNAETRE

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=308, Percent_Identity=26.6233766233766, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789202, Length=322, Percent_Identity=29.1925465838509, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788474, Length=318, Percent_Identity=29.2452830188679, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790194, Length=314, Percent_Identity=27.0700636942675, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787948, Length=317, Percent_Identity=26.813880126183, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789456, Length=319, Percent_Identity=26.6457680250784, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786540, Length=318, Percent_Identity=25.1572327044025, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI48994940, Length=347, Percent_Identity=25.0720461095101, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789068, Length=313, Percent_Identity=26.1980830670927, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787580, Length=331, Percent_Identity=23.2628398791541, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790689, Length=336, Percent_Identity=25.5952380952381, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=25.625, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36570; Mature: 36570

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQKIRRPRQADIATLAGVSVSTVSRVLANEPGISETVRRHILKVAAEHGYPVKSATEA
CCCHHHCCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
VAGGLALIASDGVTGGLSVFYEAIVDGLRAGAAEAGMPFEIRMVREDRTTPDAVRDYMRA
HHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHCCCCEEEEEECCCCCCHHHHHHHHHH
ASAEGLFLVGIDPNEMLRAWLLDSMTPAVLVNGTDPMMQLDGVSPANFFGAYQATSRLMK
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHH
AGHRRILHLSGSHRHTIRERIRGFEAAIAAVPGAEGRFVALALQGSASGEAHERTAAILA
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHC
ENAGFTAAFCMNDFIAVGVLEAVTEAGLRVPEDFAIVGFDDLPCALMTNPQLSTMRVDRA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHH
ALGREAISLMLSRFRNRTAPARHICQAVIPVPGGTVPNAETRE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNEQKIRRPRQADIATLAGVSVSTVSRVLANEPGISETVRRHILKVAAEHGYPVKSATEA
CCCHHHCCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
VAGGLALIASDGVTGGLSVFYEAIVDGLRAGAAEAGMPFEIRMVREDRTTPDAVRDYMRA
HHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHCCCCEEEEEECCCCCCHHHHHHHHHH
ASAEGLFLVGIDPNEMLRAWLLDSMTPAVLVNGTDPMMQLDGVSPANFFGAYQATSRLMK
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHH
AGHRRILHLSGSHRHTIRERIRGFEAAIAAVPGAEGRFVALALQGSASGEAHERTAAILA
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHC
ENAGFTAAFCMNDFIAVGVLEAVTEAGLRVPEDFAIVGFDDLPCALMTNPQLSTMRVDRA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHH
ALGREAISLMLSRFRNRTAPARHICQAVIPVPGGTVPNAETRE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA