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The map label for this gene is hisH [H]

Identifier: 209551550

GI number: 209551550

Start: 4131360

End: 4132010

Strand: Reverse

Name: hisH [H]

Synonym: Rleg2_3979

Alternate gene names: 209551550

Gene position: 4132010-4131360 (Counterclockwise)

Preceding gene: 209551551

Following gene: 209551549

Centisome position: 91.05

GC content: 62.06

Gene sequence:

>651_bases
ATGCGCGTCGCGATTATCGATTATGGTTCCGGCAATCTGCGCTCGGCGACCAAGGCTTTCGAACGGGCCGCCCGTGAAGC
GGGCATCGATGCGCATATCGATCTCACCGACAAGGCCGAAAATGTTGCCGCCGCCGATCGTATCGTGCTTCCCGGCGTCG
GCGCCTATGCCGATTGCCGGCGCGGTCTTGATGCCGTGCCCGATATGGCCGAGGTGCTGATCGAAGCTGTCGAGAAGAAG
GCCCGGCCGTTCCTCGGAATCTGCGTCGGCATGCAGCTGATGTCCTCGCGCGGCCTCGAGAAGACCGTGACGCACGGTTT
TAACTGGATCCCCGGCGATGTTGTCGAGATGACGCCGGACGATCCCGCGCTGAAGATCCCGCAGATCGGCTGGAACACGC
TCGACCTGCAGCGCGAACATCCGTTGTTCGACGGCATTCCGACGGGGTCCGAGGGGCTGCATGCCTATTTCGTGCATTCC
TATCATCTTGCCGCCGAGAACGCCGACGATGTCATTGCGACCGTCGATTATGGCGGTGCGATGACGGCTCTGGTCGGGCG
CGACAACATGGTCGGCGCCCAGTTTCACCCGGAGAAGAGCCAGAAGCTCGGCCTGGCGCTGATTGCCAATTTCCTGCGCT
GGAACCCGTAA

Upstream 100 bases:

>100_bases
ACGCCGTCACACCGGACTGGGAGAACAAGACCCGTCCGAACAATCCGCCTGGCCAGGCCACCTCGCTTGGTCTCTTTGGT
TTTGATGGAGGACGCTGACG

Downstream 100 bases:

>100_bases
TACCGCCGCCTTATTTCCCCTTTATGGAAACGGTGGAACAGGAGTGCGCTCGATGCCGGCTTCGCGGAAATCAGGCAAGG
TGTTCTATATGCTGAGTCCC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MRVAIIDYGSGNLRSATKAFERAAREAGIDAHIDLTDKAENVAAADRIVLPGVGAYADCRRGLDAVPDMAEVLIEAVEKK
ARPFLGICVGMQLMSSRGLEKTVTHGFNWIPGDVVEMTPDDPALKIPQIGWNTLDLQREHPLFDGIPTGSEGLHAYFVHS
YHLAAENADDVIATVDYGGAMTALVGRDNMVGAQFHPEKSQKLGLALIANFLRWNP

Sequences:

>Translated_216_residues
MRVAIIDYGSGNLRSATKAFERAAREAGIDAHIDLTDKAENVAAADRIVLPGVGAYADCRRGLDAVPDMAEVLIEAVEKK
ARPFLGICVGMQLMSSRGLEKTVTHGFNWIPGDVVEMTPDDPALKIPQIGWNTLDLQREHPLFDGIPTGSEGLHAYFVHS
YHLAAENADDVIATVDYGGAMTALVGRDNMVGAQFHPEKSQKLGLALIANFLRWNP
>Mature_216_residues
MRVAIIDYGSGNLRSATKAFERAAREAGIDAHIDLTDKAENVAAADRIVLPGVGAYADCRRGLDAVPDMAEVLIEAVEKK
ARPFLGICVGMQLMSSRGLEKTVTHGFNWIPGDVVEMTPDDPALKIPQIGWNTLDLQREHPLFDGIPTGSEGLHAYFVHS
YHLAAENADDVIATVDYGGAMTALVGRDNMVGAQFHPEKSQKLGLALIANFLRWNP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=213, Percent_Identity=36.6197183098592, Blast_Score=115, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319725, Length=225, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23393; Mature: 23393

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAIIDYGSGNLRSATKAFERAAREAGIDAHIDLTDKAENVAAADRIVLPGVGAYADCR
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCEEEECCCCCHHHHH
RGLDAVPDMAEVLIEAVEKKARPFLGICVGMQLMSSRGLEKTVTHGFNWIPGDVVEMTPD
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEECCC
DPALKIPQIGWNTLDLQREHPLFDGIPTGSEGLHAYFVHSYHLAAENADDVIATVDYGGA
CCCEECCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCE
MTALVGRDNMVGAQFHPEKSQKLGLALIANFLRWNP
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVAIIDYGSGNLRSATKAFERAAREAGIDAHIDLTDKAENVAAADRIVLPGVGAYADCR
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCEEEECCCCCHHHHH
RGLDAVPDMAEVLIEAVEKKARPFLGICVGMQLMSSRGLEKTVTHGFNWIPGDVVEMTPD
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEECCC
DPALKIPQIGWNTLDLQREHPLFDGIPTGSEGLHAYFVHSYHLAAENADDVIATVDYGGA
CCCEECCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEECCCE
MTALVGRDNMVGAQFHPEKSQKLGLALIANFLRWNP
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]