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The map label for this gene is hisF [H]

Identifier: 209551546

GI number: 209551546

Start: 4129029

End: 4129817

Strand: Reverse

Name: hisF [H]

Synonym: Rleg2_3975

Alternate gene names: 209551546

Gene position: 4129817-4129029 (Counterclockwise)

Preceding gene: 209551547

Following gene: 209551545

Centisome position: 91.01

GC content: 63.62

Gene sequence:

>789_bases
ATGACCCTCAAGGCCCGTATCATCCCCTGCCTCGACGTCAAGGACGGCCGCGTCGTCAAGGGCGTTAACTTTCTCAATCT
CGTCGATGCCGGCGATCCCGTCGAGGCGGCGAAGGCCTATGATGCGGCCGGCGCCGACGAACTCTGCTTCCTCGACATTA
CCGCTTCCTCGGACAATCGCGAGACGATCTTCGATGTCGTATCGCGCACGGCCGACCAATGCTTCATGCCGCTGACGGTC
GGCGGCGGGGTGCGCACCATCGCCGATATCCGCAAGCTCCTGCTGTGCGGCGCCGACAAGGTCTCGATCAATTCGGCGGC
GGTCAGCAATCCCGACTTCGTCACCGAGGCGGCCGACAAGTTCGGCGACCAGTGCATCGTCGTCTCGATCGACGCCAAGC
GCAGACGTACGCAGGCGGTCGGCGGCGACAATCTCAGCGCCTGGGAAATCTATACGCATGGCGGCCGCAATGCGACCGGC
ATCGATGCCGTCGAATTCGCCCAGAAGATGGTCGAGCGCGGCGCCGGCGAATTGCTGGTCACTTCGATGGACCGCGACGG
CACCAAGGTCGGCTACGATCTGGAGCTGACGCGGGCGATTGCCGATGCGGTGCGTGTGCCCGTCATCGCCTCTGGCGGCG
TCGGCGACCTCGACGATCTCGTCGCCGGGGTGAAAGAGGGCCATGCCAACGCCGTTCTCGCCGCATCGATCTTCCACTTC
GGCACCTATTCCGTCGGAGAAGCGAAGCACTATATGTCGAAGTGCGGCATCGACATGCGTCTCGACTGA

Upstream 100 bases:

>100_bases
CGAAGGGGCAATTTCAGGCCGCGCGCTTTACGACGGCCGTATCGACCCCGCGGAAGCGCTGGCGCTGATCAAGGCCAGCA
GGGCAAAGGAGACTGCGTAA

Downstream 100 bases:

>100_bases
ACCTTGAAAGCGGACATATGAGCGGATTTTCCCTTTCCGATCTCGAAAGCATCGTCGCAGAGCGCTCGACAGCCTCGCCG
GAGCAATCCTGGACAGCGAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTLKARIIPCLDVKDGRVVKGVNFLNLVDAGDPVEAAKAYDAAGADELCFLDITASSDNRETIFDVVSRTADQCFMPLTV
GGGVRTIADIRKLLLCGADKVSINSAAVSNPDFVTEAADKFGDQCIVVSIDAKRRRTQAVGGDNLSAWEIYTHGGRNATG
IDAVEFAQKMVERGAGELLVTSMDRDGTKVGYDLELTRAIADAVRVPVIASGGVGDLDDLVAGVKEGHANAVLAASIFHF
GTYSVGEAKHYMSKCGIDMRLD

Sequences:

>Translated_262_residues
MTLKARIIPCLDVKDGRVVKGVNFLNLVDAGDPVEAAKAYDAAGADELCFLDITASSDNRETIFDVVSRTADQCFMPLTV
GGGVRTIADIRKLLLCGADKVSINSAAVSNPDFVTEAADKFGDQCIVVSIDAKRRRTQAVGGDNLSAWEIYTHGGRNATG
IDAVEFAQKMVERGAGELLVTSMDRDGTKVGYDLELTRAIADAVRVPVIASGGVGDLDDLVAGVKEGHANAVLAASIFHF
GTYSVGEAKHYMSKCGIDMRLD
>Mature_261_residues
TLKARIIPCLDVKDGRVVKGVNFLNLVDAGDPVEAAKAYDAAGADELCFLDITASSDNRETIFDVVSRTADQCFMPLTVG
GGVRTIADIRKLLLCGADKVSINSAAVSNPDFVTEAADKFGDQCIVVSIDAKRRRTQAVGGDNLSAWEIYTHGGRNATGI
DAVEFAQKMVERGAGELLVTSMDRDGTKVGYDLELTRAIADAVRVPVIASGGVGDLDDLVAGVKEGHANAVLAASIFHFG
TYSVGEAKHYMSKCGIDMRLD

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=266, Percent_Identity=40.9774436090226, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI87082028, Length=252, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=31.4285714285714, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27747; Mature: 27616

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKARIIPCLDVKDGRVVKGVNFLNLVDAGDPVEAAKAYDAAGADELCFLDITASSDNR
CCCCEEEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCH
ETIFDVVSRTADQCFMPLTVGGGVRTIADIRKLLLCGADKVSINSAAVSNPDFVTEAADK
HHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHH
FGDQCIVVSIDAKRRRTQAVGGDNLSAWEIYTHGGRNATGIDAVEFAQKMVERGAGELLV
HCCEEEEEEECCCHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEE
TSMDRDGTKVGYDLELTRAIADAVRVPVIASGGVGDLDDLVAGVKEGHANAVLAASIFHF
EECCCCCCEECCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCCCEEHHHHHHHH
GTYSVGEAKHYMSKCGIDMRLD
CCCCCHHHHHHHHHCCCCEECC
>Mature Secondary Structure 
TLKARIIPCLDVKDGRVVKGVNFLNLVDAGDPVEAAKAYDAAGADELCFLDITASSDNR
CCCEEEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCH
ETIFDVVSRTADQCFMPLTVGGGVRTIADIRKLLLCGADKVSINSAAVSNPDFVTEAADK
HHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHH
FGDQCIVVSIDAKRRRTQAVGGDNLSAWEIYTHGGRNATGIDAVEFAQKMVERGAGELLV
HCCEEEEEEECCCHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEE
TSMDRDGTKVGYDLELTRAIADAVRVPVIASGGVGDLDDLVAGVKEGHANAVLAASIFHF
EECCCCCCEECCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCCCEEHHHHHHHH
GTYSVGEAKHYMSKCGIDMRLD
CCCCCHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]