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The map label for this gene is trmE [H]

Identifier: 209551502

GI number: 209551502

Start: 4081410

End: 4082723

Strand: Reverse

Name: trmE [H]

Synonym: Rleg2_3931

Alternate gene names: 209551502

Gene position: 4082723-4081410 (Counterclockwise)

Preceding gene: 209551503

Following gene: 209551501

Centisome position: 89.97

GC content: 58.6

Gene sequence:

>1314_bases
ATGCTGCATGATACGATCTATGCGCTATCGAGCGGTGCCACACCCTCAGGTGTGTCGGTCGTTCGGATCAGCGGCCCGTT
GACCAAAGACATATTGGTCGGGCTCGTCGGATCAGTACCGACAGCCCGATATGCCGTACGGCGAACGATTCGGAATCGTA
ACAACCAGCCGATCGACAGCGGCCTCGTATTGTTTTTTCCTGGGCCACACTCGTTTACCGGCGAAGACGTCGCCGAACTG
CAGCTACATGGCAGCAAAGCCGTGCTGGCGGCGCTGTTTGAGGCCCTTTGTGAATTGCCAGGCGTTCGGATGGCGGTTGA
GGGCGAGTTCTCCCGCCGCGCTTTTGAAAATGGCAAGCTTGATCTCGTCGAAGTCGAAGGCCTAGCCGATCTTATCGGCG
CGGAAACCGAGATGCAGCGGCGTCTTGCGGTCGAACAGAGCGCAGGGGGGCTTTCCCTGATTTACGATTCGTGGGCGGAA
CGATTAACGCGTGCCCGCGCGTTGATCGAGGCGGAACTTGATTTTCCCGACGAGGATGATGTTCCAGGCTCGGTATCCGA
CATGGTCTGGGCTGAGATGGAAAGGCTGCGCCAAGATATCGGGCAGCACCTCTCGGCAGCCTCGGCGGGAGAGATTATCC
GGGACGGCTTCAAGGTGGTCATCGCCGGGGCGCCCAACGCAGGGAAATCCAGCCTGCTGAACGCTCTCGCACGACGCGAC
GTGGCGATTGTCACTGATATCGCCGGAACTACCCGCGATGTTCTGCAGGTCGATCTCGACATTGATGGCTACCTGGTCAA
GCTCTATGACACGGCGGGTCTTCGTGAGGCGGATGACCGGGTTGAAATGGAGGGCGTGCGGCGTGCACGTGTCGCGCTCC
GTGATGCCGATCTCGTCTTGCTGCTGGTCGACATGACGAACCCCCTGCTCCCTGACGACTTGGACCAGGCTGCACCACAT
GTTACTGTCGGGACGAAGAAGGATCTTGTGGATGTCGTCTCGGGTCATTACGACCTGCAGATCTCGACGGCAACGGGTGA
AGGCTTGTCGGAATTGCGGCAGGTGATCGGCAACAGCGTCGCCACGCGGTTTACCGGCCTCTCCATGGCTATTCCGAGCC
GCCAACGGCATAAAGATTCGCTGGCGAAATGTTTGACGGCGCTTGAGATCGCCATCTCGGCGACGGATGTCAATCTCGAG
TTGCGCACTGAACAGCTTCGCATTGCTGCGGACTATTTGGGCAGAATTACCGGCAGAGTGGATGTTGAGCAGCTGCTCGG
CGTGATCTTCTCAGAGTTTTGTATCGGCAAGTGA

Upstream 100 bases:

>100_bases
GATTCATCCTATATAGAAGCTGGAGGCATCCGAACGATGTCGCCAGCTTTTCTGTTAGGCAGGATCGATTCGGGCTCGAA
CCGAAACAGGCGATATGGCC

Downstream 100 bases:

>100_bases
TTCACGTGAAACACCGGGGTATATTGCCGCGGTTTCACCTGAAACGACGGGTGGGTTGGAGTATCGGATGACCGATAAAG
TCTATGATGTCATTGTGATC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MLHDTIYALSSGATPSGVSVVRISGPLTKDILVGLVGSVPTARYAVRRTIRNRNNQPIDSGLVLFFPGPHSFTGEDVAEL
QLHGSKAVLAALFEALCELPGVRMAVEGEFSRRAFENGKLDLVEVEGLADLIGAETEMQRRLAVEQSAGGLSLIYDSWAE
RLTRARALIEAELDFPDEDDVPGSVSDMVWAEMERLRQDIGQHLSAASAGEIIRDGFKVVIAGAPNAGKSSLLNALARRD
VAIVTDIAGTTRDVLQVDLDIDGYLVKLYDTAGLREADDRVEMEGVRRARVALRDADLVLLLVDMTNPLLPDDLDQAAPH
VTVGTKKDLVDVVSGHYDLQISTATGEGLSELRQVIGNSVATRFTGLSMAIPSRQRHKDSLAKCLTALEIAISATDVNLE
LRTEQLRIAADYLGRITGRVDVEQLLGVIFSEFCIGK

Sequences:

>Translated_437_residues
MLHDTIYALSSGATPSGVSVVRISGPLTKDILVGLVGSVPTARYAVRRTIRNRNNQPIDSGLVLFFPGPHSFTGEDVAEL
QLHGSKAVLAALFEALCELPGVRMAVEGEFSRRAFENGKLDLVEVEGLADLIGAETEMQRRLAVEQSAGGLSLIYDSWAE
RLTRARALIEAELDFPDEDDVPGSVSDMVWAEMERLRQDIGQHLSAASAGEIIRDGFKVVIAGAPNAGKSSLLNALARRD
VAIVTDIAGTTRDVLQVDLDIDGYLVKLYDTAGLREADDRVEMEGVRRARVALRDADLVLLLVDMTNPLLPDDLDQAAPH
VTVGTKKDLVDVVSGHYDLQISTATGEGLSELRQVIGNSVATRFTGLSMAIPSRQRHKDSLAKCLTALEIAISATDVNLE
LRTEQLRIAADYLGRITGRVDVEQLLGVIFSEFCIGK
>Mature_437_residues
MLHDTIYALSSGATPSGVSVVRISGPLTKDILVGLVGSVPTARYAVRRTIRNRNNQPIDSGLVLFFPGPHSFTGEDVAEL
QLHGSKAVLAALFEALCELPGVRMAVEGEFSRRAFENGKLDLVEVEGLADLIGAETEMQRRLAVEQSAGGLSLIYDSWAE
RLTRARALIEAELDFPDEDDVPGSVSDMVWAEMERLRQDIGQHLSAASAGEIIRDGFKVVIAGAPNAGKSSLLNALARRD
VAIVTDIAGTTRDVLQVDLDIDGYLVKLYDTAGLREADDRVEMEGVRRARVALRDADLVLLLVDMTNPLLPDDLDQAAPH
VTVGTKKDLVDVVSGHYDLQISTATGEGLSELRQVIGNSVATRFTGLSMAIPSRQRHKDSLAKCLTALEIAISATDVNLE
LRTEQLRIAADYLGRITGRVDVEQLLGVIFSEFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=460, Percent_Identity=42.8260869565217, Blast_Score=311, Evalue=6e-85,
Organism=Homo sapiens, GI193082993, Length=444, Percent_Identity=43.018018018018, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI193082988, Length=492, Percent_Identity=40.4471544715447, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI2367268, Length=453, Percent_Identity=40.1766004415011, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=32.6839826839827, Blast_Score=208, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=35.4969574036511, Blast_Score=262, Evalue=7e-71,
Organism=Drosophila melanogaster, GI45550813, Length=483, Percent_Identity=39.1304347826087, Blast_Score=308, Evalue=4e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 47222; Mature: 47222

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHDTIYALSSGATPSGVSVVRISGPLTKDILVGLVGSVPTARYAVRRTIRNRNNQPIDS
CCCCCEEHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
GLVLFFPGPHSFTGEDVAELQLHGSKAVLAALFEALCELPGVRMAVEGEFSRRAFENGKL
CEEEEECCCCCCCCCCHHHEEECCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCE
DLVEVEGLADLIGAETEMQRRLAVEQSAGGLSLIYDSWAERLTRARALIEAELDFPDEDD
EEEEECCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
VPGSVSDMVWAEMERLRQDIGQHLSAASAGEIIRDGFKVVIAGAPNAGKSSLLNALARRD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCC
VAIVTDIAGTTRDVLQVDLDIDGYLVKLYDTAGLREADDRVEMEGVRRARVALRDADLVL
EEEEEECCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
LLVDMTNPLLPDDLDQAAPHVTVGTKKDLVDVVSGHYDLQISTATGEGLSELRQVIGNSV
EEEECCCCCCCCCHHHCCCEEECCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCHH
ATRFTGLSMAIPSRQRHKDSLAKCLTALEIAISATDVNLELRTEQLRIAADYLGRITGRV
HHHHCCCEEECCCHHHHHHHHHHHHHHHHHEEEEEECEEEEEHHHHHHHHHHHHHHCCCC
DVEQLLGVIFSEFCIGK
CHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLHDTIYALSSGATPSGVSVVRISGPLTKDILVGLVGSVPTARYAVRRTIRNRNNQPIDS
CCCCCEEHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
GLVLFFPGPHSFTGEDVAELQLHGSKAVLAALFEALCELPGVRMAVEGEFSRRAFENGKL
CEEEEECCCCCCCCCCHHHEEECCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCE
DLVEVEGLADLIGAETEMQRRLAVEQSAGGLSLIYDSWAERLTRARALIEAELDFPDEDD
EEEEECCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
VPGSVSDMVWAEMERLRQDIGQHLSAASAGEIIRDGFKVVIAGAPNAGKSSLLNALARRD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCC
VAIVTDIAGTTRDVLQVDLDIDGYLVKLYDTAGLREADDRVEMEGVRRARVALRDADLVL
EEEEEECCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
LLVDMTNPLLPDDLDQAAPHVTVGTKKDLVDVVSGHYDLQISTATGEGLSELRQVIGNSV
EEEECCCCCCCCCHHHCCCEEECCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCHH
ATRFTGLSMAIPSRQRHKDSLAKCLTALEIAISATDVNLELRTEQLRIAADYLGRITGRV
HHHHCCCEEECCCHHHHHHHHHHHHHHHHHEEEEEECEEEEEHHHHHHHHHHHHHHCCCC
DVEQLLGVIFSEFCIGK
CHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA