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The map label for this gene is mnmG [H]

Identifier: 209551501

GI number: 209551501

Start: 4079465

End: 4081342

Strand: Reverse

Name: mnmG [H]

Synonym: Rleg2_3930

Alternate gene names: 209551501

Gene position: 4081342-4079465 (Counterclockwise)

Preceding gene: 209551502

Following gene: 209551500

Centisome position: 89.94

GC content: 59.96

Gene sequence:

>1878_bases
ATGACCGATAAAGTCTATGATGTCATTGTGATCGGTGGTGGCCATGCGGGGTCGGAAGCTGCCAGTGCGGCCGCGCGCCT
TGGCGCCAAAACCGCTTTGGTGACACATAGACGCGATACGATCGGGGTGATGTCCTGCAATCCGGCGATCGGCGGTCTCG
GCAAGGGCCATTTGGTTCGCGAGATCGACGCGATGGACGGGCTCATGGGTCGCGTCGCCGATGTCGCCGGCATCCAGTTC
AGGATGCTGAACAAGAAAAAAGGTGCGGCCGTGCGCGGACCGCGGACCCAGGCCGATCGAAAACTTTATCGTTTGGCGAT
GTTGGCGGCGATCGAGGCCACAGCAGGTTTGGATATCGTCGAAGGCGACGCCTTTGATCTGAAAGTCGTCGATGGCCGCG
TGGCCGGGGTGATCATGCAGGACGGCCGCGTGCTTGGAGCGGCCGCCGTTGTTCTGACGACCGGGACCTTTCTTCGCGGC
CTCATTCATATCGGTTCAGAAAAGACGCCGGCTGGTCGCGTTGGCGAAGCACCGTCGATCGGGCTGTCGGCCACCCTGGC
GAGGCTTGGATTGCGGCTGGGACGGTTGAAGACCGGGACGCCGGCGCGGCTGGATGGAAAGACCATCGACTGGCAGGCCG
TTGGCCGGCAAGGGGCCGACGAGGAGCTTACCCCCTTCTCGCTGATGACCGATGCAATTACCACTCCGCAGATCGAATGC
GGGGTCACCCGGACGACCGAGGCGACACACCGCATCATCGTCGACAACATCATGCGGTCGGCCATGTATTCCGGCCAGAT
CGAAGGGGTGGGTCCGCGCTATTGTCCGTCGATCGAAGACAAGCTCGTTAAGTTCGGAGAGCGGGACGGACATCAGGTCT
TTCTCGAGCCAGAAGGTCTCGACGATGATACCGTCTATCCAAACGGGATCTCGACATCTCTGCCGGCAGAGGTCCAGGCC
GAATTCATTAAGACGATTCCCGGGCTTGAGAGGGCGAGAATCATTCAGCCGGGTTACGCGATCGAATATGATCATGTCGA
TCCGAGAGAGCTGACACCCTCGCTCGAGGTCAAACGGCTGAAAGGCCTGTTTCTCGCCGGTCAGATCAATGGCACCACGG
GTTACGAGGAAGCGGCCGCCCAAGGCCTGGCCGCCGGAATCAATGCTGCATTGCGAAGCAGCGATTCGGATCCGTTTCAT
TTCAGCCGCACAAATTCCTATATCGGCGTGATGATCGATGACTTGACGTCGCGCGGCGTTACGGAACCGTATCGAATGTT
TACATCGCGTGCGGAATATCGGCTGATGCTAAGGGCTGACAATGCCGATATGCGGCTGACGCCGTTGGCGATGCAGCTCG
GATGTGTGAGCGGCGAACGAGAGCGGCGATTCACACGCTATCAGACAGAGATCGAAGCGGGTCGAGTGCTGCTGCAGTCA
CTGGCGGTGACGCCGAACGAAGCGCGGCGCGTCGGTTTGAACATCAATCTCGACGGCCAGCGCCGAACGGCTTATGACCT
GCTCTCCTATCCGAATTATGATTTTGATGTCCTTCGGAAGGTGTGGCCTGACGAGCTGGGGGCGGTTGCCGGAAAAGTCG
CGGAGGCATTGGAAATCGAAGCGGGCTATTCGGTCTATCTCGACCGGCAGGCCGCGGCGATTGCCGATCAGCAGCGCGAC
GAGGACCGGCATATACCGGCTGATTTCGACTATACCGCGCTGTCCGGCCTTTCGAACGAGCTTAAGGCAAAGCTCGGCGC
TGCTCGGCCTTTCAACATTGCCCAGGCCGCAATCGTTGAAGGGATGACGCCTGCGGCCGTCGCACTATTGCTCGTGCATC
TGCGTCGGCGCAACACCTCCGAGCGATATAGCGCCTAG

Upstream 100 bases:

>100_bases
GTGATCTTCTCAGAGTTTTGTATCGGCAAGTGATTCACGTGAAACACCGGGGTATATTGCCGCGGTTTCACCTGAAACGA
CGGGTGGGTTGGAGTATCGG

Downstream 100 bases:

>100_bases
ATACAAGATATTGAGAGTGAGAATGGAATTAAACGGCTTACGTGTTTCACGTGAAACACAGGAACGGCTGCAACACTTCG
CGGCGCTCTTTCAGAAATGG

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MTDKVYDVIVIGGGHAGSEAASAAARLGAKTALVTHRRDTIGVMSCNPAIGGLGKGHLVREIDAMDGLMGRVADVAGIQF
RMLNKKKGAAVRGPRTQADRKLYRLAMLAAIEATAGLDIVEGDAFDLKVVDGRVAGVIMQDGRVLGAAAVVLTTGTFLRG
LIHIGSEKTPAGRVGEAPSIGLSATLARLGLRLGRLKTGTPARLDGKTIDWQAVGRQGADEELTPFSLMTDAITTPQIEC
GVTRTTEATHRIIVDNIMRSAMYSGQIEGVGPRYCPSIEDKLVKFGERDGHQVFLEPEGLDDDTVYPNGISTSLPAEVQA
EFIKTIPGLERARIIQPGYAIEYDHVDPRELTPSLEVKRLKGLFLAGQINGTTGYEEAAAQGLAAGINAALRSSDSDPFH
FSRTNSYIGVMIDDLTSRGVTEPYRMFTSRAEYRLMLRADNADMRLTPLAMQLGCVSGERERRFTRYQTEIEAGRVLLQS
LAVTPNEARRVGLNINLDGQRRTAYDLLSYPNYDFDVLRKVWPDELGAVAGKVAEALEIEAGYSVYLDRQAAAIADQQRD
EDRHIPADFDYTALSGLSNELKAKLGAARPFNIAQAAIVEGMTPAAVALLLVHLRRRNTSERYSA

Sequences:

>Translated_625_residues
MTDKVYDVIVIGGGHAGSEAASAAARLGAKTALVTHRRDTIGVMSCNPAIGGLGKGHLVREIDAMDGLMGRVADVAGIQF
RMLNKKKGAAVRGPRTQADRKLYRLAMLAAIEATAGLDIVEGDAFDLKVVDGRVAGVIMQDGRVLGAAAVVLTTGTFLRG
LIHIGSEKTPAGRVGEAPSIGLSATLARLGLRLGRLKTGTPARLDGKTIDWQAVGRQGADEELTPFSLMTDAITTPQIEC
GVTRTTEATHRIIVDNIMRSAMYSGQIEGVGPRYCPSIEDKLVKFGERDGHQVFLEPEGLDDDTVYPNGISTSLPAEVQA
EFIKTIPGLERARIIQPGYAIEYDHVDPRELTPSLEVKRLKGLFLAGQINGTTGYEEAAAQGLAAGINAALRSSDSDPFH
FSRTNSYIGVMIDDLTSRGVTEPYRMFTSRAEYRLMLRADNADMRLTPLAMQLGCVSGERERRFTRYQTEIEAGRVLLQS
LAVTPNEARRVGLNINLDGQRRTAYDLLSYPNYDFDVLRKVWPDELGAVAGKVAEALEIEAGYSVYLDRQAAAIADQQRD
EDRHIPADFDYTALSGLSNELKAKLGAARPFNIAQAAIVEGMTPAAVALLLVHLRRRNTSERYSA
>Mature_624_residues
TDKVYDVIVIGGGHAGSEAASAAARLGAKTALVTHRRDTIGVMSCNPAIGGLGKGHLVREIDAMDGLMGRVADVAGIQFR
MLNKKKGAAVRGPRTQADRKLYRLAMLAAIEATAGLDIVEGDAFDLKVVDGRVAGVIMQDGRVLGAAAVVLTTGTFLRGL
IHIGSEKTPAGRVGEAPSIGLSATLARLGLRLGRLKTGTPARLDGKTIDWQAVGRQGADEELTPFSLMTDAITTPQIECG
VTRTTEATHRIIVDNIMRSAMYSGQIEGVGPRYCPSIEDKLVKFGERDGHQVFLEPEGLDDDTVYPNGISTSLPAEVQAE
FIKTIPGLERARIIQPGYAIEYDHVDPRELTPSLEVKRLKGLFLAGQINGTTGYEEAAAQGLAAGINAALRSSDSDPFHF
SRTNSYIGVMIDDLTSRGVTEPYRMFTSRAEYRLMLRADNADMRLTPLAMQLGCVSGERERRFTRYQTEIEAGRVLLQSL
AVTPNEARRVGLNINLDGQRRTAYDLLSYPNYDFDVLRKVWPDELGAVAGKVAEALEIEAGYSVYLDRQAAAIADQQRDE
DRHIPADFDYTALSGLSNELKAKLGAARPFNIAQAAIVEGMTPAAVALLLVHLRRRNTSERYSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=639, Percent_Identity=46.9483568075117, Blast_Score=544, Evalue=1e-155,
Organism=Homo sapiens, GI19882217, Length=664, Percent_Identity=45.1807228915663, Blast_Score=530, Evalue=1e-150,
Organism=Homo sapiens, GI183227703, Length=679, Percent_Identity=44.1826215022091, Blast_Score=525, Evalue=1e-149,
Organism=Escherichia coli, GI2367273, Length=621, Percent_Identity=51.8518518518518, Blast_Score=619, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI17534255, Length=616, Percent_Identity=43.0194805194805, Blast_Score=491, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6321202, Length=634, Percent_Identity=44.4794952681388, Blast_Score=533, Evalue=1e-152,
Organism=Drosophila melanogaster, GI24658174, Length=632, Percent_Identity=46.6772151898734, Blast_Score=539, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 67582; Mature: 67451

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKVYDVIVIGGGHAGSEAASAAARLGAKTALVTHRRDTIGVMSCNPAIGGLGKGHLVR
CCCCEEEEEEEECCCCCCHHHHHHHHHCCHHEEEEECCCEEEEEECCCCCCCCCCCHHHH
EIDAMDGLMGRVADVAGIQFRMLNKKKGAAVRGPRTQADRKLYRLAMLAAIEATAGLDIV
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
EGDAFDLKVVDGRVAGVIMQDGRVLGAAAVVLTTGTFLRGLIHIGSEKTPAGRVGEAPSI
ECCCEEEEEECCEEEEEEEECCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GLSATLARLGLRLGRLKTGTPARLDGKTIDWQAVGRQGADEELTPFSLMTDAITTPQIEC
CHHHHHHHHHHHHCCCCCCCCCCCCCCEECHHHHCCCCCCCCCCCHHHHHHCCCCCCEEC
GVTRTTEATHRIIVDNIMRSAMYSGQIEGVGPRYCPSIEDKLVKFGERDGHQVFLEPEGL
CCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCC
DDDTVYPNGISTSLPAEVQAEFIKTIPGLERARIIQPGYAIEYDHVDPRELTPSLEVKRL
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHEEECCCCEEEECCCCHHHCCCCHHHHHH
KGLFLAGQINGTTGYEEAAAQGLAAGINAALRSSDSDPFHFSRTNSYIGVMIDDLTSRGV
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECHHCCCC
TEPYRMFTSRAEYRLMLRADNADMRLTPLAMQLGCVSGERERRFTRYQTEIEAGRVLLQS
CHHHHHHHCCCCEEEEEEECCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LAVTPNEARRVGLNINLDGQRRTAYDLLSYPNYDFDVLRKVWPDELGAVAGKVAEALEIE
HCCCCCHHHEEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHEEC
AGYSVYLDRQAAAIADQQRDEDRHIPADFDYTALSGLSNELKAKLGAARPFNIAQAAIVE
CCCEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHC
GMTPAAVALLLVHLRRRNTSERYSA
CCCHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
TDKVYDVIVIGGGHAGSEAASAAARLGAKTALVTHRRDTIGVMSCNPAIGGLGKGHLVR
CCCEEEEEEEECCCCCCHHHHHHHHHCCHHEEEEECCCEEEEEECCCCCCCCCCCHHHH
EIDAMDGLMGRVADVAGIQFRMLNKKKGAAVRGPRTQADRKLYRLAMLAAIEATAGLDIV
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
EGDAFDLKVVDGRVAGVIMQDGRVLGAAAVVLTTGTFLRGLIHIGSEKTPAGRVGEAPSI
ECCCEEEEEECCEEEEEEEECCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GLSATLARLGLRLGRLKTGTPARLDGKTIDWQAVGRQGADEELTPFSLMTDAITTPQIEC
CHHHHHHHHHHHHCCCCCCCCCCCCCCEECHHHHCCCCCCCCCCCHHHHHHCCCCCCEEC
GVTRTTEATHRIIVDNIMRSAMYSGQIEGVGPRYCPSIEDKLVKFGERDGHQVFLEPEGL
CCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCC
DDDTVYPNGISTSLPAEVQAEFIKTIPGLERARIIQPGYAIEYDHVDPRELTPSLEVKRL
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHEEECCCCEEEECCCCHHHCCCCHHHHHH
KGLFLAGQINGTTGYEEAAAQGLAAGINAALRSSDSDPFHFSRTNSYIGVMIDDLTSRGV
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECHHCCCC
TEPYRMFTSRAEYRLMLRADNADMRLTPLAMQLGCVSGERERRFTRYQTEIEAGRVLLQS
CHHHHHHHCCCCEEEEEEECCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LAVTPNEARRVGLNINLDGQRRTAYDLLSYPNYDFDVLRKVWPDELGAVAGKVAEALEIE
HCCCCCHHHEEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHEEC
AGYSVYLDRQAAAIADQQRDEDRHIPADFDYTALSGLSNELKAKLGAARPFNIAQAAIVE
CCCEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHC
GMTPAAVALLLVHLRRRNTSERYSA
CCCHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA