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The map label for this gene is gidB

Identifier: 209551500

GI number: 209551500

Start: 4078825

End: 4079442

Strand: Reverse

Name: gidB

Synonym: Rleg2_3929

Alternate gene names: 209551500

Gene position: 4079442-4078825 (Counterclockwise)

Preceding gene: 209551501

Following gene: 209551499

Centisome position: 89.9

GC content: 56.63

Gene sequence:

>618_bases
ATGGAATTAAACGGCTTACGTGTTTCACGTGAAACACAGGAACGGCTGCAACACTTCGCGGCGCTCTTTCAGAAATGGGC
AAAAACCATCAATCTGGTGGCGCCGTCGACGCTCGACGATCTCTGGAACCGGCACATCGCCGACAGCAGCCAAGTGTTTC
AAATCCATCCCAAGCCGGTGATCTGGGCCGACCTCGGAAGCGGCGGCGGTTTTCCAGGCGTCATCACGGCGATCTTCCTG
GCCGAACTGCAGGACGGATGGGTGCATCTGGTCGAGAGCAATCACAAGAAGGCCGCTTTCCTCCGAACAGCGCTGCGGGA
AACAAATGCCCGCGGCAGCGTGCACAGCAGCCGGATCGAAGATGCCCATGCGGAGATCGGGGAATGCGGGGCGATTTCGG
CCCGGGCGCTCGCCGATCTTGACGGACTCCTCGAATATTCTGCTCCCTGGATGCTGGGCGAAAAAGATTGCCGCGGCTTT
TTTCATAAAGGCCGGGATTACCTGAGGGAAATCGACAAAGCACGTGGTCGGTGGGAATTCGATCTGTTAGAACATAGTAG
CGCCGTCGAGCAGGAATCTGTCATTCTGGAAATATCGAATCTCCGGCGCTTGGTTTAA

Upstream 100 bases:

>100_bases
GGGATGACGCCTGCGGCCGTCGCACTATTGCTCGTGCATCTGCGTCGGCGCAACACCTCCGAGCGATATAGCGCCTAGAT
ACAAGATATTGAGAGTGAGA

Downstream 100 bases:

>100_bases
CAGATCGGATATTGCGGCAATGGCTGGCGAACGACATCGGATTATCACCATCGCAAATCAAAAGGGTGGCGTTGGCAAAA
CGACCACCGCTATCAACCTG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MELNGLRVSRETQERLQHFAALFQKWAKTINLVAPSTLDDLWNRHIADSSQVFQIHPKPVIWADLGSGGGFPGVITAIFL
AELQDGWVHLVESNHKKAAFLRTALRETNARGSVHSSRIEDAHAEIGECGAISARALADLDGLLEYSAPWMLGEKDCRGF
FHKGRDYLREIDKARGRWEFDLLEHSSAVEQESVILEISNLRRLV

Sequences:

>Translated_205_residues
MELNGLRVSRETQERLQHFAALFQKWAKTINLVAPSTLDDLWNRHIADSSQVFQIHPKPVIWADLGSGGGFPGVITAIFL
AELQDGWVHLVESNHKKAAFLRTALRETNARGSVHSSRIEDAHAEIGECGAISARALADLDGLLEYSAPWMLGEKDCRGF
FHKGRDYLREIDKARGRWEFDLLEHSSAVEQESVILEISNLRRLV
>Mature_205_residues
MELNGLRVSRETQERLQHFAALFQKWAKTINLVAPSTLDDLWNRHIADSSQVFQIHPKPVIWADLGSGGGFPGVITAIFL
AELQDGWVHLVESNHKKAAFLRTALRETNARGSVHSSRIEDAHAEIGECGAISARALADLDGLLEYSAPWMLGEKDCRGF
FHKGRDYLREIDKARGRWEFDLLEHSSAVEQESVILEISNLRRLV

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_RHILW (B5ZV45)

Other databases:

- EMBL:   CP001191
- RefSeq:   YP_002283417.1
- ProteinModelPortal:   B5ZV45
- SMR:   B5ZV45
- GeneID:   6982693
- GenomeReviews:   CP001191_GR
- KEGG:   rlt:Rleg2_3929
- HOGENOM:   HBG686577
- OMA:   ARGRWEF
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23082; Mature: 23082

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNGLRVSRETQERLQHFAALFQKWAKTINLVAPSTLDDLWNRHIADSSQVFQIHPKPV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCE
IWADLGSGGGFPGVITAIFLAELQDGWVHLVESNHKKAAFLRTALRETNARGSVHSSRIE
EEEECCCCCCCHHHHHHHHHHHHHCHHEEEHHCCCHHHHHHHHHHHHCCCCCCHHHHHHH
DAHAEIGECGAISARALADLDGLLEYSAPWMLGEKDCRGFFHKGRDYLREIDKARGRWEF
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEH
DLLEHSSAVEQESVILEISNLRRLV
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELNGLRVSRETQERLQHFAALFQKWAKTINLVAPSTLDDLWNRHIADSSQVFQIHPKPV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCE
IWADLGSGGGFPGVITAIFLAELQDGWVHLVESNHKKAAFLRTALRETNARGSVHSSRIE
EEEECCCCCCCHHHHHHHHHHHHHCHHEEEHHCCCHHHHHHHHHHHHCCCCCCHHHHHHH
DAHAEIGECGAISARALADLDGLLEYSAPWMLGEKDCRGFFHKGRDYLREIDKARGRWEF
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEH
DLLEHSSAVEQESVILEISNLRRLV
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA