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The map label for this gene is rsmB [H]

Identifier: 209551488

GI number: 209551488

Start: 4064899

End: 4066287

Strand: Reverse

Name: rsmB [H]

Synonym: Rleg2_3917

Alternate gene names: 209551488

Gene position: 4066287-4064899 (Counterclockwise)

Preceding gene: 209551489

Following gene: 209551487

Centisome position: 89.61

GC content: 64.94

Gene sequence:

>1389_bases
ATGGTCTTGAATTCAGACGGCACGAAGAAACCATTCCGCAAGCACAAGCGCTCCACCGAGCGATCCGCGCCTGTAAAGCC
CGGCCTGCAGGCGCGGGCTGCAGCGGCAAAAATTCTCGGCGCTGTCGTCGAGCGCAAACTGCCGCTCGACGGTGCCCTCG
ATCATGAACACGGCAATCCGGCCTACAAGGCGCTTGGCGAAAGCGACCGGGCGCTTGTCCGCGCCATTCTGAACACGACG
CTGCGTCATCTGCCGCGTATCGATGCCGCCATAAGCTCGCTGCTGGAATCGCCGCTGCCGGAGGGGGCACGGGCGCTGCA
GCACGTGCTTGCGATCGGCGTCGCGCAGATCCTCTATCTCGATGTGCCCGACCATTCGGCCGTCGATCTTGCCGTCGAGC
AGGCCAATCAGGATCCGCGCAACCGGCGTTTCGCCAAGCTGGTCAATGCCGTTCTTCGCCGGCTCGGCCGCGAGAAGGAT
GAGGTGCTCGGCGAGATCGACAAGGTCGCACCGATGCCGGCCTGGTTCATCGCCAGGCTTGAGAAAGCCTATGGCCGGGA
CGCGGCGCTGGCGATTTCGGAATCTCAGCTCCAACCGGCCGCAATCGATCTGACCGTCAAGTCGGATGCCGAAGGCTGGG
CAAAGCGGCTGAACGGCGTTGTGCTGCCGACCGGCGGCGTGCGGCTCGCCGCCTTCGATGGCGGCATCCCGTCGCTCGAA
GGCTTCGACGATGGCGCCTGGTGGGTGCAGGATGCGGCCGCAAGCATTCCGGCCAAGCTGTTCGGCGACCTCTCCGGTAA
GCGCGCCGCCGATCTCTGCGCCGCACCAGGCGGCAAGACGGCGCAGCTCATTCTTGCCGGCGGCGCGGTCACCGCACTCG
ACCAGTCGGAAAGTCGGCTGAGACGGCTGCGGTCGAATCTCGACCGGCTCAGCCTGAAAGCAGAGACGATCGCGGCGGAT
CTGACGACATTCGAACCGGCCGAGCGTTTCGATGCAATCCTGCTCGATGCGCCCTGCTCTTCCACCGGCACGACACGCCG
GCACCCCGACGTGTTGTGGACCAAGGGACCCGAGGATATCGGCAGGCTGGCGGCGCTGCAGGAGCGGCTGCTGCGTCATG
CGCTGACATTGCTGAAGCCGGATGGAACGCTGGTTTTTTCGAATTGTTCGCTGGATCCTACCGAAGGCGAAGAGGTCGTT
GCCCGCGTTCTTGCCGATACGGATGGGATCGAGCGCGTTCGGATCGATGCCGGCGACTGGCCCGGCCTTGAAGCGGCGAT
CACGCCGCTCGGCGAATTCCGCACGCTTCCGACCATGCTGAAAATGCCCGATGGCATCGCCTCCGGTCTCGACGGCTTTT
ATGCCGCCATGCTGCGGCGCGTGGCCTGA

Upstream 100 bases:

>100_bases
TCTCGACGCCGCCGGTTCGGGCTGCTAATCCGTCGCGCAAATCGCGCTCTGTTCCGGATACGGGTCTTGGTCGCGGTGGT
TCGCAACCGCCAAAAGGTCC

Downstream 100 bases:

>100_bases
TTGCGGGCTTACGCGCGTCGTGTCACGTCTGCCCACGCAGGACGCTTTACAGCCGTGCTCGAACGCGGGTGAAACACGTT
AATTTGCGTATATTCGAACA

Product: Fmu (Sun) domain-containing protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MVLNSDGTKKPFRKHKRSTERSAPVKPGLQARAAAAKILGAVVERKLPLDGALDHEHGNPAYKALGESDRALVRAILNTT
LRHLPRIDAAISSLLESPLPEGARALQHVLAIGVAQILYLDVPDHSAVDLAVEQANQDPRNRRFAKLVNAVLRRLGREKD
EVLGEIDKVAPMPAWFIARLEKAYGRDAALAISESQLQPAAIDLTVKSDAEGWAKRLNGVVLPTGGVRLAAFDGGIPSLE
GFDDGAWWVQDAAASIPAKLFGDLSGKRAADLCAAPGGKTAQLILAGGAVTALDQSESRLRRLRSNLDRLSLKAETIAAD
LTTFEPAERFDAILLDAPCSSTGTTRRHPDVLWTKGPEDIGRLAALQERLLRHALTLLKPDGTLVFSNCSLDPTEGEEVV
ARVLADTDGIERVRIDAGDWPGLEAAITPLGEFRTLPTMLKMPDGIASGLDGFYAAMLRRVA

Sequences:

>Translated_462_residues
MVLNSDGTKKPFRKHKRSTERSAPVKPGLQARAAAAKILGAVVERKLPLDGALDHEHGNPAYKALGESDRALVRAILNTT
LRHLPRIDAAISSLLESPLPEGARALQHVLAIGVAQILYLDVPDHSAVDLAVEQANQDPRNRRFAKLVNAVLRRLGREKD
EVLGEIDKVAPMPAWFIARLEKAYGRDAALAISESQLQPAAIDLTVKSDAEGWAKRLNGVVLPTGGVRLAAFDGGIPSLE
GFDDGAWWVQDAAASIPAKLFGDLSGKRAADLCAAPGGKTAQLILAGGAVTALDQSESRLRRLRSNLDRLSLKAETIAAD
LTTFEPAERFDAILLDAPCSSTGTTRRHPDVLWTKGPEDIGRLAALQERLLRHALTLLKPDGTLVFSNCSLDPTEGEEVV
ARVLADTDGIERVRIDAGDWPGLEAAITPLGEFRTLPTMLKMPDGIASGLDGFYAAMLRRVA
>Mature_462_residues
MVLNSDGTKKPFRKHKRSTERSAPVKPGLQARAAAAKILGAVVERKLPLDGALDHEHGNPAYKALGESDRALVRAILNTT
LRHLPRIDAAISSLLESPLPEGARALQHVLAIGVAQILYLDVPDHSAVDLAVEQANQDPRNRRFAKLVNAVLRRLGREKD
EVLGEIDKVAPMPAWFIARLEKAYGRDAALAISESQLQPAAIDLTVKSDAEGWAKRLNGVVLPTGGVRLAAFDGGIPSLE
GFDDGAWWVQDAAASIPAKLFGDLSGKRAADLCAAPGGKTAQLILAGGAVTALDQSESRLRRLRSNLDRLSLKAETIAAD
LTTFEPAERFDAILLDAPCSSTGTTRRHPDVLWTKGPEDIGRLAALQERLLRHALTLLKPDGTLVFSNCSLDPTEGEEVV
ARVLADTDGIERVRIDAGDWPGLEAAITPLGEFRTLPTMLKMPDGIASGLDGFYAAMLRRVA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI32698918, Length=253, Percent_Identity=31.2252964426877, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI76150625, Length=249, Percent_Identity=30.5220883534137, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI76150623, Length=249, Percent_Identity=30.5220883534137, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI23199998, Length=230, Percent_Identity=30.8695652173913, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI8922322, Length=230, Percent_Identity=30.8695652173913, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI270288816, Length=231, Percent_Identity=31.1688311688312, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI270288818, Length=230, Percent_Identity=30.8695652173913, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI39995082, Length=197, Percent_Identity=30.9644670050761, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI301336155, Length=197, Percent_Identity=30.9644670050761, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI11545785, Length=176, Percent_Identity=30.1136363636364, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI2367212, Length=409, Percent_Identity=32.2738386308068, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87081985, Length=228, Percent_Identity=35.9649122807018, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17536757, Length=254, Percent_Identity=29.1338582677165, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25143471, Length=182, Percent_Identity=35.1648351648352, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71998419, Length=251, Percent_Identity=28.6852589641434, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115534021, Length=251, Percent_Identity=28.6852589641434, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17509941, Length=177, Percent_Identity=27.1186440677966, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319447, Length=197, Percent_Identity=36.5482233502538, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324268, Length=248, Percent_Identity=29.0322580645161, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355201, Length=199, Percent_Identity=33.6683417085427, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI22024126, Length=250, Percent_Identity=28.8, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24668781, Length=272, Percent_Identity=30.5147058823529, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356513, Length=272, Percent_Identity=30.8823529411765, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920494, Length=174, Percent_Identity=29.8850574712644, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 49596; Mature: 49596

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLNSDGTKKPFRKHKRSTERSAPVKPGLQARAAAAKILGAVVERKLPLDGALDHEHGNP
CCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
AYKALGESDRALVRAILNTTLRHLPRIDAAISSLLESPLPEGARALQHVLAIGVAQILYL
HHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEE
DVPDHSAVDLAVEQANQDPRNRRFAKLVNAVLRRLGREKDEVLGEIDKVAPMPAWFIARL
ECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHH
EKAYGRDAALAISESQLQPAAIDLTVKSDAEGWAKRLNGVVLPTGGVRLAAFDGGIPSLE
HHHHCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHCCEEECCCCEEEEEECCCCCCCC
GFDDGAWWVQDAAASIPAKLFGDLSGKRAADLCAAPGGKTAQLILAGGAVTALDQSESRL
CCCCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCCEEECCCHHHHH
RRLRSNLDRLSLKAETIAADLTTFEPAERFDAILLDAPCSSTGTTRRHPDVLWTKGPEDI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCEEEEECCCCCCCCCCCCCCCEECCCHHHH
GRLAALQERLLRHALTLLKPDGTLVFSNCSLDPTEGEEVVARVLADTDGIERVRIDAGDW
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCC
PGLEAAITPLGEFRTLPTMLKMPDGIASGLDGFYAAMLRRVA
CCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVLNSDGTKKPFRKHKRSTERSAPVKPGLQARAAAAKILGAVVERKLPLDGALDHEHGNP
CCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
AYKALGESDRALVRAILNTTLRHLPRIDAAISSLLESPLPEGARALQHVLAIGVAQILYL
HHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEE
DVPDHSAVDLAVEQANQDPRNRRFAKLVNAVLRRLGREKDEVLGEIDKVAPMPAWFIARL
ECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHH
EKAYGRDAALAISESQLQPAAIDLTVKSDAEGWAKRLNGVVLPTGGVRLAAFDGGIPSLE
HHHHCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHCCEEECCCCEEEEEECCCCCCCC
GFDDGAWWVQDAAASIPAKLFGDLSGKRAADLCAAPGGKTAQLILAGGAVTALDQSESRL
CCCCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCCEEECCCHHHHH
RRLRSNLDRLSLKAETIAADLTTFEPAERFDAILLDAPCSSTGTTRRHPDVLWTKGPEDI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCEEEEECCCCCCCCCCCCCCCEECCCHHHH
GRLAALQERLLRHALTLLKPDGTLVFSNCSLDPTEGEEVVARVLADTDGIERVRIDAGDW
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCC
PGLEAAITPLGEFRTLPTMLKMPDGIASGLDGFYAAMLRRVA
CCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]