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The map label for this gene is proB [H]

Identifier: 209551446

GI number: 209551446

Start: 4016217

End: 4017386

Strand: Direct

Name: proB [H]

Synonym: Rleg2_3875

Alternate gene names: 209551446

Gene position: 4016217-4017386 (Clockwise)

Preceding gene: 209551445

Following gene: 209551447

Centisome position: 88.5

GC content: 67.01

Gene sequence:

>1170_bases
ATGACCAGCCGTAAGCCGCTTGGACGCTACCGCCGCATCGTCATCAAGATCGGCTCGGCGCTGCTGGTCGACCGCAAGGC
CGGCCTGAAGAAGGCCTGGCTCGACGCCATGTGCGCCGATATTTCAGGTTTGAAGGCCAAGGGCATCGATGTGCTCGTCG
TGTCCTCCGGCGCCATCGCGCTCGGCCGCTCGGTGCTCGACCTTCCCTCCGGCGCGCTGAAACTGGAGGAAAGCCAGGCG
GCTGCCGCCGTCGGCCAGATCGCGCTTGCGCGCGCCTGGTCGGAAAGCCTGTCGCGCGGCGAGATCGTCGCCGGCCAGAT
CCTGCTGACGCTCGGCGACACCGAGGAGCGCCGCCGCTATCTCAACGCGCGCGCCACCATCAATCAGCTTCTGAAAATCG
GCGCCGTGCCGATCATCAACGAGAACGACACGGTCGCCACCAGCGAAATCCGCTATGGCGACAATGATCGCCTGGCCGCC
CGCGTTGCGACGATGACCGGCGCCGACCTGCTCGTCCTTCTCTCCGATATCGACGGGCTCTACACCGCACCGCCGCATCT
CGACCCGAACGCGACATTCCTGGAGACGATTTCCGAAATTACCCCGGAGATCGAAGCGATGGCAGGGGGCGCGGCCTCCG
AACTCTCGCGCGGCGGCATGCGCACCAAGATCGATGCCGGCAAGATCGCGACGACATCCGGCTGCGCGATGATCATCGCC
TCCGGCAAGACCGACAGCCCGCTGTCGGCGATCGAAAACGGCGCGCGTTCCTCCTGGTTCGCCCCGTCGGGCACGCCGGT
GACTGCCCGCAAGACCTGGATCGCCGGACAGCTGCAGCCGGCGGGCGGACTGCATGTCGATGACGGCGCCGTCACGGCCC
TCGGCGCCGGCAAGAGCCTGCTTCCCGCCGGCGTGCGCAGCATTTCCGGCCTGTTCAGCCGCGGCGATACGGTGGCGATC
ATCGGTCCCGCCGGCCGCGAGATCGCCCGTGGCCTTGTCAGCTATGATGCCGAGGACGCCCGCCGGATCGCCGGTCGCAA
GTCGGCGGAGATCGAGGCCATTCTCGGTTATCCCGGCCGCGCCGCCATGGTCCATCGCGACGACATGGTGATGACCGCGC
AGATCGGATCGAAATCGGAAAGGCAGAAGAAGGACGCGGCCCATGCTTGA

Upstream 100 bases:

>100_bases
GAGGAAAATGCCGAGGAAAAGCCGGCTAAGGTGCCGAAGCAGCGCCACCGCGACATTGTTGTCGCCGATGAGGGCGAGGA
CGTTGGCGAGGATGGGGCCG

Downstream 100 bases:

>100_bases
TACCGTTGCGCTAAGCCCCGAGATTGACGCGCTGATGAACGACATCGGCCGCAAGGCAAAGGCTGCCGCGCGACCACTGA
GCTTTGCGTCCACCGAGGTG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTSRKPLGRYRRIVIKIGSALLVDRKAGLKKAWLDAMCADISGLKAKGIDVLVVSSGAIALGRSVLDLPSGALKLEESQA
AAAVGQIALARAWSESLSRGEIVAGQILLTLGDTEERRRYLNARATINQLLKIGAVPIINENDTVATSEIRYGDNDRLAA
RVATMTGADLLVLLSDIDGLYTAPPHLDPNATFLETISEITPEIEAMAGGAASELSRGGMRTKIDAGKIATTSGCAMIIA
SGKTDSPLSAIENGARSSWFAPSGTPVTARKTWIAGQLQPAGGLHVDDGAVTALGAGKSLLPAGVRSISGLFSRGDTVAI
IGPAGREIARGLVSYDAEDARRIAGRKSAEIEAILGYPGRAAMVHRDDMVMTAQIGSKSERQKKDAAHA

Sequences:

>Translated_389_residues
MTSRKPLGRYRRIVIKIGSALLVDRKAGLKKAWLDAMCADISGLKAKGIDVLVVSSGAIALGRSVLDLPSGALKLEESQA
AAAVGQIALARAWSESLSRGEIVAGQILLTLGDTEERRRYLNARATINQLLKIGAVPIINENDTVATSEIRYGDNDRLAA
RVATMTGADLLVLLSDIDGLYTAPPHLDPNATFLETISEITPEIEAMAGGAASELSRGGMRTKIDAGKIATTSGCAMIIA
SGKTDSPLSAIENGARSSWFAPSGTPVTARKTWIAGQLQPAGGLHVDDGAVTALGAGKSLLPAGVRSISGLFSRGDTVAI
IGPAGREIARGLVSYDAEDARRIAGRKSAEIEAILGYPGRAAMVHRDDMVMTAQIGSKSERQKKDAAHA
>Mature_388_residues
TSRKPLGRYRRIVIKIGSALLVDRKAGLKKAWLDAMCADISGLKAKGIDVLVVSSGAIALGRSVLDLPSGALKLEESQAA
AAVGQIALARAWSESLSRGEIVAGQILLTLGDTEERRRYLNARATINQLLKIGAVPIINENDTVATSEIRYGDNDRLAAR
VATMTGADLLVLLSDIDGLYTAPPHLDPNATFLETISEITPEIEAMAGGAASELSRGGMRTKIDAGKIATTSGCAMIIAS
GKTDSPLSAIENGARSSWFAPSGTPVTARKTWIAGQLQPAGGLHVDDGAVTALGAGKSLLPAGVRSISGLFSRGDTVAII
GPAGREIARGLVSYDAEDARRIAGRKSAEIEAILGYPGRAAMVHRDDMVMTAQIGSKSERQKKDAAHA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=273, Percent_Identity=30.03663003663, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI62912457, Length=271, Percent_Identity=30.2583025830258, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=42.5, Blast_Score=260, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17569967, Length=260, Percent_Identity=32.6923076923077, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=260, Percent_Identity=32.6923076923077, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6320506, Length=412, Percent_Identity=31.7961165048544, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321822, Length=420, Percent_Identity=32.6190476190476, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21357643, Length=297, Percent_Identity=28.956228956229, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40600; Mature: 40469

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRKPLGRYRRIVIKIGSALLVDRKAGLKKAWLDAMCADISGLKAKGIDVLVVSSGAIA
CCCCCCHHHHHEEHHEECCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHH
LGRSVLDLPSGALKLEESQAAAAVGQIALARAWSESLSRGEIVAGQILLTLGDTEERRRY
HCCHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHH
LNARATINQLLKIGAVPIINENDTVATSEIRYGDNDRLAARVATMTGADLLVLLSDIDGL
HHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCCCEEEEHHHCCCHHEEEEHHCCCC
YTAPPHLDPNATFLETISEITPEIEAMAGGAASELSRGGMRTKIDAGKIATTSGCAMIIA
CCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHCCCCEEEECCCCEEECCCCEEEEE
SGKTDSPLSAIENGARSSWFAPSGTPVTARKTWIAGQLQPAGGLHVDDGAVTALGAGKSL
CCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEECCCCEEEECCCCHH
LPAGVRSISGLFSRGDTVAIIGPAGREIARGLVSYDAEDARRIAGRKSAEIEAILGYPGR
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCC
AAMVHRDDMVMTAQIGSKSERQKKDAAHA
EEEEECCCEEEEEECCCCHHHHHHHCCCC
>Mature Secondary Structure 
TSRKPLGRYRRIVIKIGSALLVDRKAGLKKAWLDAMCADISGLKAKGIDVLVVSSGAIA
CCCCCHHHHHEEHHEECCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHH
LGRSVLDLPSGALKLEESQAAAAVGQIALARAWSESLSRGEIVAGQILLTLGDTEERRRY
HCCHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHH
LNARATINQLLKIGAVPIINENDTVATSEIRYGDNDRLAARVATMTGADLLVLLSDIDGL
HHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCCCEEEEHHHCCCHHEEEEHHCCCC
YTAPPHLDPNATFLETISEITPEIEAMAGGAASELSRGGMRTKIDAGKIATTSGCAMIIA
CCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHCCCCEEEECCCCEEECCCCEEEEE
SGKTDSPLSAIENGARSSWFAPSGTPVTARKTWIAGQLQPAGGLHVDDGAVTALGAGKSL
CCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEECCCCEEEECCCCHH
LPAGVRSISGLFSRGDTVAIIGPAGREIARGLVSYDAEDARRIAGRKSAEIEAILGYPGR
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCC
AAMVHRDDMVMTAQIGSKSERQKKDAAHA
EEEEECCCEEEEEECCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA