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The map label for this gene is gcvA [H]

Identifier: 209551358

GI number: 209551358

Start: 3915089

End: 3915991

Strand: Direct

Name: gcvA [H]

Synonym: Rleg2_3787

Alternate gene names: 209551358

Gene position: 3915089-3915991 (Clockwise)

Preceding gene: 209551351

Following gene: 209551359

Centisome position: 86.27

GC content: 66.11

Gene sequence:

>903_bases
ATGTTCGCTTCAAGACGTTTTCTGCCGTCGATTTCACATCTCGCCGCCTTCGAAGCCGTTGCCCGCACCGGTAGCGTCAC
GGCGGCCGCCCGTGAACTCGATCTGACGCAGAGCGCCGTCAGCCGCCAGGTGAGCGCGCTGGAGGAGCAGCTTGGCGTCG
AGCTCTTCCTGCGCGAACGTCAGACCATGCGGCTGACGCCGGCCGGCGACAGTTATGCCCGCGAGATCCGCGAGGCGCTG
CGGCGCATATCGAGCGCTTCGCTGAACCTGCGCGCCAATCCGCATGGCGGCACGCTCAATCTCGCCATCCTGCCGACCTT
CGGCACGCGCTGGCTGGCGCCGCGCCTCGGGCGCTTCCTCGCCGCCAATCCCGGCGTGACGATCAATCTGGTGACCCGGC
TTTCGCCTTTCGATTTCCGCCTCGATTCGATCGACGCCGCCATCCATTTCGGCCATCCGCACTGGCCGGGCGCCGAACTT
GCCTTTCTGATGTCGGAGCGCACAGTGCCGGCCTGCAGCCCCGATTTTCTGAAGCGATACACGATCTCCGGGCCGCAGGA
TCTGCTCTCCGTTCCGCTGCTGCATCTGACGACGCGGCCCGATGCCTGGGAGCAATGGTTCGCCGGCAACGGCGTGTCTT
TCGAAAGCGTGCACGGCATGCTCTTCGACCAGTTCGCCACCGCTGCGCAAGCGGCGATCGCCGGTCTCGGCATCGCTCTT
TTGCCGACCTTTCTGATGCAGGAAGAGTTGCGGCGCGGCGATCTCGTCGCCGCGGTCGACCGCGAAATGGAAAGCCGCGA
GCGCTATTATCTCGCCTTTCCGCCCGAGCGCGCCGACTATGCGCCGCTTGCCGCCTTCCGCGACTGGATTGTCGCGGAAG
CGGCCGCAGAGCCGGCGGCGTGA

Upstream 100 bases:

>100_bases
TCCGGCTCATTCCGTTTTTGCAGGATGAGCCTCTTTACAACTGTCATAAAGGCGGAAATATCGACATTTTGGAAATAGCT
CATTCTGAAATGGAATGAAC

Downstream 100 bases:

>100_bases
CGAACGGCTTGCATCGGCATGGCGCTTTCGACCATTATATCAGCCAAACAACCCTTTGCAGGAAATCCCCATGAAGCCGA
TCTTCGTCCAGCTCCAATGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MFASRRFLPSISHLAAFEAVARTGSVTAAARELDLTQSAVSRQVSALEEQLGVELFLRERQTMRLTPAGDSYAREIREAL
RRISSASLNLRANPHGGTLNLAILPTFGTRWLAPRLGRFLAANPGVTINLVTRLSPFDFRLDSIDAAIHFGHPHWPGAEL
AFLMSERTVPACSPDFLKRYTISGPQDLLSVPLLHLTTRPDAWEQWFAGNGVSFESVHGMLFDQFATAAQAAIAGLGIAL
LPTFLMQEELRRGDLVAAVDREMESRERYYLAFPPERADYAPLAAFRDWIVAEAAAEPAA

Sequences:

>Translated_300_residues
MFASRRFLPSISHLAAFEAVARTGSVTAAARELDLTQSAVSRQVSALEEQLGVELFLRERQTMRLTPAGDSYAREIREAL
RRISSASLNLRANPHGGTLNLAILPTFGTRWLAPRLGRFLAANPGVTINLVTRLSPFDFRLDSIDAAIHFGHPHWPGAEL
AFLMSERTVPACSPDFLKRYTISGPQDLLSVPLLHLTTRPDAWEQWFAGNGVSFESVHGMLFDQFATAAQAAIAGLGIAL
LPTFLMQEELRRGDLVAAVDREMESRERYYLAFPPERADYAPLAAFRDWIVAEAAAEPAA
>Mature_300_residues
MFASRRFLPSISHLAAFEAVARTGSVTAAARELDLTQSAVSRQVSALEEQLGVELFLRERQTMRLTPAGDSYAREIREAL
RRISSASLNLRANPHGGTLNLAILPTFGTRWLAPRLGRFLAANPGVTINLVTRLSPFDFRLDSIDAAIHFGHPHWPGAEL
AFLMSERTVPACSPDFLKRYTISGPQDLLSVPLLHLTTRPDAWEQWFAGNGVSFESVHGMLFDQFATAAQAAIAGLGIAL
LPTFLMQEELRRGDLVAAVDREMESRERYYLAFPPERADYAPLAAFRDWIVAEAAAEPAA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=36.986301369863, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786448, Length=282, Percent_Identity=31.2056737588652, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=30.6896551724138, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=29.4520547945205, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789639, Length=255, Percent_Identity=30.5882352941176, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=28.1879194630872, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790262, Length=268, Percent_Identity=29.8507462686567, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=34.375, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787589, Length=299, Percent_Identity=25.0836120401338, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789506, Length=91, Percent_Identity=40.6593406593407, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=25.6055363321799, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693105, Length=257, Percent_Identity=25.6809338521401, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789204, Length=131, Percent_Identity=35.8778625954198, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=256, Percent_Identity=25.390625, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787601, Length=127, Percent_Identity=28.3464566929134, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33059; Mature: 33059

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFASRRFLPSISHLAAFEAVARTGSVTAAARELDLTQSAVSRQVSALEEQLGVELFLRER
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC
QTMRLTPAGDSYAREIREALRRISSASLNLRANPHGGTLNLAILPTFGTRWLAPRLGRFL
CEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHH
AANPGVTINLVTRLSPFDFRLDSIDAAIHFGHPHWPGAELAFLMSERTVPACSPDFLKRY
CCCCCCEEEEEEECCCCCEECCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHH
TISGPQDLLSVPLLHLTTRPDAWEQWFAGNGVSFESVHGMLFDQFATAAQAAIAGLGIAL
CCCCHHHHHHCCHHEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPTFLMQEELRRGDLVAAVDREMESRERYYLAFPPERADYAPLAAFRDWIVAEAAAEPAA
HHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFASRRFLPSISHLAAFEAVARTGSVTAAARELDLTQSAVSRQVSALEEQLGVELFLRER
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC
QTMRLTPAGDSYAREIREALRRISSASLNLRANPHGGTLNLAILPTFGTRWLAPRLGRFL
CEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHH
AANPGVTINLVTRLSPFDFRLDSIDAAIHFGHPHWPGAELAFLMSERTVPACSPDFLKRY
CCCCCCEEEEEEECCCCCEECCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHH
TISGPQDLLSVPLLHLTTRPDAWEQWFAGNGVSFESVHGMLFDQFATAAQAAIAGLGIAL
CCCCHHHHHHCCHHEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPTFLMQEELRRGDLVAAVDREMESRERYYLAFPPERADYAPLAAFRDWIVAEAAAEPAA
HHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]