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The map label for this gene is amtB [H]

Identifier: 209551340

GI number: 209551340

Start: 3893185

End: 3894618

Strand: Reverse

Name: amtB [H]

Synonym: Rleg2_3769

Alternate gene names: 209551340

Gene position: 3894618-3893185 (Counterclockwise)

Preceding gene: 209551341

Following gene: 209551339

Centisome position: 85.82

GC content: 63.32

Gene sequence:

>1434_bases
ATGTCGATTTCGAAGTTTTCTTCCCCCTTTGCGCTGATCTGCGCAACGTCGGCCGCGCTTCTGGCGCCGGTTGTCGCTTT
CGCGCAGGAAGCTGCGCCCGCCGCCGCCACCACCGCTGCTGCCGCTCCTGCCTTCACCATGGACAAGGGTGACAATGCCT
GGATGCTGATCTCCTCGGCACTCGTCCTGCTGATGACCATCCCCGGCCTTGCGCTGTTCTACGGCGGTCTCGTGCGCGCC
AAGAACATGCTCTCCGTGCTGATGCAGGTGTTCATGATCACCGCCGTCGTGGCGCTGCTCTGGGTCACCTACGGCTATTC
GCTCGCCTTCACCGACGGCGGCTCGCTGAACAGCTTCGTCGGCGGCTTCTCAAAGGCGTTCCTGGCCGGCGTCAATACCT
CGTCGCTCGCCGAAACCTTCTCGAAGGGCGTCGCCATTCCGGAATACACCTTCATCGTCTTCCAGATGACCTTCGCCTGC
ATCACGCCCGGCCTGATCGTCGGCGCATTCGCCGAGCGCATCAAGTTCTCGGCCGTCATGCTCTTCGTCGTGCTCTGGGT
CACCTTCATCTACTTCCCCATGGCGCACATGGTCTGGTTCTGGGGCGGTCCGAGCTCCTACACCTCGCCGGCCGGCCTGA
TCTTCTCCTACGGCGCAATCGACTTCGCCGGCGGCACGGTCGTCCACATCAATGCCGGTATCGCCGGCCTGGTCGGCGCC
ATCATGCTCGGCAAGCGCACCGGCTATAAGAAGGAAATCATGGCTCCGCATTCGATGACCCTGACCATGGTCGGCGCATC
GCTGCTCTGGGTCGGCTGGTTCGGCTTCAACGCCGGCTCCAACCTCGAAGCCAATGGCTACGCCTCGCTTGCCTTTATCA
ACACCTTCGTCGCGACGGCTGCTGCCGCCGTCTCCTGGTGCATTGTCGAAAGCCTGACCCGCGGCAAGGCTTCGATGCTC
GGCGGCGCTTCCGGTGCGGTTGCCGGTCTCGTCGCCATCACCCCGGCTGCCGGTTTTGCCGGCCCGATGGGCTCGATCGT
TCTCGGCCTTATCGTCTCGCCGGTCTGCTACTTCTTCGTCGACGTCGTCAAGAACAAGTTCAACTACGACGACAGCCTCG
ACGTCTTCGGCGTGCATTGCGTCGGCGGTATCCTCGGCGCACTCGGCACCGGTATCCTCGTCAACCCGGCGCTCGGCGGT
GCAGGCATTGTCGATTATTCCACGGCCGATTTCGCTGCCTCCTATGCCGGCACGGCAACCCAGGTCTTGGCCCAGGCCAA
GGGCGTTCTGACGACGCTTCTGTGGTCGGGCATCGGTTCGGCGATCCTCTACAAGATCGTCGACGTCGTCATCGGCCTGC
GCGTGAGCGTCGAAGCCGAGCGCGAAGGTCTCGACCTTTCGACCCACGGCGAAGCCGCCTACCACGCCTCTTGA

Upstream 100 bases:

>100_bases
GGCAAGATTTTCGTCTATTCGATCGACCATGCCGTGCGCATCCGTACCGGCGAAACCGATTCAGAAGCGCTGTAAGCGGC
AGATCAAGGAGCTTTTTTCA

Downstream 100 bases:

>100_bases
TCGCAGCCTTTGCGGCGCCCGGCACAAGGGCGCCGCTTCACGGCCCGTCGCGGCCTATCTAGAGCTTTTCCGGGTTAGAT
CCGGAAAAGCTGTCATAGAC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSISKFSSPFALICATSAALLAPVVAFAQEAAPAAATTAAAAPAFTMDKGDNAWMLISSALVLLMTIPGLALFYGGLVRA
KNMLSVLMQVFMITAVVALLWVTYGYSLAFTDGGSLNSFVGGFSKAFLAGVNTSSLAETFSKGVAIPEYTFIVFQMTFAC
ITPGLIVGAFAERIKFSAVMLFVVLWVTFIYFPMAHMVWFWGGPSSYTSPAGLIFSYGAIDFAGGTVVHINAGIAGLVGA
IMLGKRTGYKKEIMAPHSMTLTMVGASLLWVGWFGFNAGSNLEANGYASLAFINTFVATAAAAVSWCIVESLTRGKASML
GGASGAVAGLVAITPAAGFAGPMGSIVLGLIVSPVCYFFVDVVKNKFNYDDSLDVFGVHCVGGILGALGTGILVNPALGG
AGIVDYSTADFAASYAGTATQVLAQAKGVLTTLLWSGIGSAILYKIVDVVIGLRVSVEAEREGLDLSTHGEAAYHAS

Sequences:

>Translated_477_residues
MSISKFSSPFALICATSAALLAPVVAFAQEAAPAAATTAAAAPAFTMDKGDNAWMLISSALVLLMTIPGLALFYGGLVRA
KNMLSVLMQVFMITAVVALLWVTYGYSLAFTDGGSLNSFVGGFSKAFLAGVNTSSLAETFSKGVAIPEYTFIVFQMTFAC
ITPGLIVGAFAERIKFSAVMLFVVLWVTFIYFPMAHMVWFWGGPSSYTSPAGLIFSYGAIDFAGGTVVHINAGIAGLVGA
IMLGKRTGYKKEIMAPHSMTLTMVGASLLWVGWFGFNAGSNLEANGYASLAFINTFVATAAAAVSWCIVESLTRGKASML
GGASGAVAGLVAITPAAGFAGPMGSIVLGLIVSPVCYFFVDVVKNKFNYDDSLDVFGVHCVGGILGALGTGILVNPALGG
AGIVDYSTADFAASYAGTATQVLAQAKGVLTTLLWSGIGSAILYKIVDVVIGLRVSVEAEREGLDLSTHGEAAYHAS
>Mature_476_residues
SISKFSSPFALICATSAALLAPVVAFAQEAAPAAATTAAAAPAFTMDKGDNAWMLISSALVLLMTIPGLALFYGGLVRAK
NMLSVLMQVFMITAVVALLWVTYGYSLAFTDGGSLNSFVGGFSKAFLAGVNTSSLAETFSKGVAIPEYTFIVFQMTFACI
TPGLIVGAFAERIKFSAVMLFVVLWVTFIYFPMAHMVWFWGGPSSYTSPAGLIFSYGAIDFAGGTVVHINAGIAGLVGAI
MLGKRTGYKKEIMAPHSMTLTMVGASLLWVGWFGFNAGSNLEANGYASLAFINTFVATAAAAVSWCIVESLTRGKASMLG
GASGAVAGLVAITPAAGFAGPMGSIVLGLIVSPVCYFFVDVVKNKFNYDDSLDVFGVHCVGGILGALGTGILVNPALGGA
GIVDYSTADFAASYAGTATQVLAQAKGVLTTLLWSGIGSAILYKIVDVVIGLRVSVEAEREGLDLSTHGEAAYHAS

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=436, Percent_Identity=50, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17550182, Length=451, Percent_Identity=28.6031042128603, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI133931100, Length=458, Percent_Identity=26.8558951965066, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17550180, Length=445, Percent_Identity=28.9887640449438, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32564042, Length=432, Percent_Identity=25.2314814814815, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324187, Length=447, Percent_Identity=33.1096196868009, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6325396, Length=431, Percent_Identity=31.7865429234339, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6321559, Length=434, Percent_Identity=32.258064516129, Blast_Score=206, Evalue=7e-54,
Organism=Drosophila melanogaster, GI161078314, Length=448, Percent_Identity=24.3303571428571, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 49291; Mature: 49160

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISKFSSPFALICATSAALLAPVVAFAQEAAPAAATTAAAAPAFTMDKGDNAWMLISSA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCCCHHHHHHHH
LVLLMTIPGLALFYGGLVRAKNMLSVLMQVFMITAVVALLWVTYGYSLAFTDGGSLNSFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHH
GGFSKAFLAGVNTSSLAETFSKGVAIPEYTFIVFQMTFACITPGLIVGAFAERIKFSAVM
HHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
LFVVLWVTFIYFPMAHMVWFWGGPSSYTSPAGLIFSYGAIDFAGGTVVHINAGIAGLVGA
HHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCCEEECCCEEEEECCCHHHHHHH
IMLGKRTGYKKEIMAPHSMTLTMVGASLLWVGWFGFNAGSNLEANGYASLAFINTFVATA
HHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
AAAVSWCIVESLTRGKASMLGGASGAVAGLVAITPAAGFAGPMGSIVLGLIVSPVCYFFV
HHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DVVKNKFNYDDSLDVFGVHCVGGILGALGTGILVNPALGGAGIVDYSTADFAASYAGTAT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCHHHHHHHCCHHH
QVLAQAKGVLTTLLWSGIGSAILYKIVDVVIGLRVSVEAEREGLDLSTHGEAAYHAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SISKFSSPFALICATSAALLAPVVAFAQEAAPAAATTAAAAPAFTMDKGDNAWMLISSA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCCCHHHHHHHH
LVLLMTIPGLALFYGGLVRAKNMLSVLMQVFMITAVVALLWVTYGYSLAFTDGGSLNSFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHH
GGFSKAFLAGVNTSSLAETFSKGVAIPEYTFIVFQMTFACITPGLIVGAFAERIKFSAVM
HHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
LFVVLWVTFIYFPMAHMVWFWGGPSSYTSPAGLIFSYGAIDFAGGTVVHINAGIAGLVGA
HHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCCEEECCCEEEEECCCHHHHHHH
IMLGKRTGYKKEIMAPHSMTLTMVGASLLWVGWFGFNAGSNLEANGYASLAFINTFVATA
HHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
AAAVSWCIVESLTRGKASMLGGASGAVAGLVAITPAAGFAGPMGSIVLGLIVSPVCYFFV
HHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DVVKNKFNYDDSLDVFGVHCVGGILGALGTGILVNPALGGAGIVDYSTADFAASYAGTAT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCHHHHHHHCCHHH
QVLAQAKGVLTTLLWSGIGSAILYKIVDVVIGLRVSVEAEREGLDLSTHGEAAYHAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]