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The map label for this gene is xthA [H]

Identifier: 209551337

GI number: 209551337

Start: 3888479

End: 3889282

Strand: Reverse

Name: xthA [H]

Synonym: Rleg2_3766

Alternate gene names: 209551337

Gene position: 3889282-3888479 (Counterclockwise)

Preceding gene: 209551338

Following gene: 209551336

Centisome position: 85.71

GC content: 61.69

Gene sequence:

>804_bases
ATGGGCTTTTCGATCACCACCTGGAACATCAACTCGGTGCGGCTGCGCATGCCGATCGTCGAGCAGCTGGTTCTCAAGCA
TCGGCCGGATATTCTCTGCCTGCAGGAAACCAAGGTGACGAACGAGCTCTTTCCGGCGGCGCCGCTGCGGGCGATGGGCT
ACGACCACATCATCATCCACGGCCAGAAAGGCTATCACGGCGTGGCGATCGCCTCGCGCATTCCGCTGACGGAGGATCAC
CGGCAGGATTATTGCGGGGTCGGCGATGCCCGCCACATCTCGGCGATCTTCGAGCACGGCAACCGCCGGATACGGCTGCA
CAATTTCTACGTCCCGGCTGGCGGCGACGAGCCGGATCGCACGATCAATCCGAAATTCGGCCACAAGCTCGATTTCATCG
AAGAGATGAAGCTCTTGAAAGCCAATGGCGAAGCCAACACCTCCGCCATTCTGGTCGGCGACCTCAACATCGCGCCGCTG
GAGCACGATGTGTGGTCGCACAAGCAGCTGCTGAAGATCGTCAGCCATACGCCTGTCGAAACCGACGGGCTGCTCGAGGT
GATGAAGCGCGGCAACTGGCTCGACCTCATGCGCCAGCATGTGCCGGAAAGCGAGAAGCTCTATACTTGGTGGAGCTACC
GCGCCAAGGACTGGGAGGCGGCCGACCGCGGCCGCCGTCTCGACCATATCTGGTCGTCATCGGATCTCGGGCCGCACCTC
AAGCGCATCGAGATCCTGAAAGAGGCACGCGGCTGGGACCGGCCCTCGGACCATGTGCCGGTCACGGCGCATTTCGATTT
CTGA

Upstream 100 bases:

>100_bases
CTTTTACACGCGGCCGGTTCCCTCCTCTGGCGCTTTGTTCTAAAGCCCTTTCATGAGAGCACGCCGTCTCAACACCCGGC
GTGCGGGAAAGGGCGTGGGC

Downstream 100 bases:

>100_bases
TGCAGTGGCTTCTCCAGGAGTTCGAAGACACCCATAAGCTGGCCGAGGCACTCGACCGGCTCGATATCCCTTACACCTGG
CACAAGGTCGTTCCGTTCAT

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MGFSITTWNINSVRLRMPIVEQLVLKHRPDILCLQETKVTNELFPAAPLRAMGYDHIIIHGQKGYHGVAIASRIPLTEDH
RQDYCGVGDARHISAIFEHGNRRIRLHNFYVPAGGDEPDRTINPKFGHKLDFIEEMKLLKANGEANTSAILVGDLNIAPL
EHDVWSHKQLLKIVSHTPVETDGLLEVMKRGNWLDLMRQHVPESEKLYTWWSYRAKDWEAADRGRRLDHIWSSSDLGPHL
KRIEILKEARGWDRPSDHVPVTAHFDF

Sequences:

>Translated_267_residues
MGFSITTWNINSVRLRMPIVEQLVLKHRPDILCLQETKVTNELFPAAPLRAMGYDHIIIHGQKGYHGVAIASRIPLTEDH
RQDYCGVGDARHISAIFEHGNRRIRLHNFYVPAGGDEPDRTINPKFGHKLDFIEEMKLLKANGEANTSAILVGDLNIAPL
EHDVWSHKQLLKIVSHTPVETDGLLEVMKRGNWLDLMRQHVPESEKLYTWWSYRAKDWEAADRGRRLDHIWSSSDLGPHL
KRIEILKEARGWDRPSDHVPVTAHFDF
>Mature_266_residues
GFSITTWNINSVRLRMPIVEQLVLKHRPDILCLQETKVTNELFPAAPLRAMGYDHIIIHGQKGYHGVAIASRIPLTEDHR
QDYCGVGDARHISAIFEHGNRRIRLHNFYVPAGGDEPDRTINPKFGHKLDFIEEMKLLKANGEANTSAILVGDLNIAPLE
HDVWSHKQLLKIVSHTPVETDGLLEVMKRGNWLDLMRQHVPESEKLYTWWSYRAKDWEAADRGRRLDHIWSSSDLGPHLK
RIEILKEARGWDRPSDHVPVTAHFDF

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=274, Percent_Identity=28.8321167883212, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI18375503, Length=274, Percent_Identity=28.8321167883212, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI18375501, Length=274, Percent_Identity=28.8321167883212, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788046, Length=274, Percent_Identity=30.2919708029197, Blast_Score=108, Evalue=3e-25,
Organism=Drosophila melanogaster, GI221330655, Length=273, Percent_Identity=30.4029304029304, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17136678, Length=273, Percent_Identity=30.4029304029304, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30730; Mature: 30599

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFSITTWNINSVRLRMPIVEQLVLKHRPDILCLQETKVTNELFPAAPLRAMGYDHIIIH
CCCEEEEEECCEEEEECHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCHHHCCCCEEEEE
GQKGYHGVAIASRIPLTEDHRQDYCGVGDARHISAIFEHGNRRIRLHNFYVPAGGDEPDR
CCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCC
TINPKFGHKLDFIEEMKLLKANGEANTSAILVGDLNIAPLEHDVWSHKQLLKIVSHTPVE
CCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
TDGLLEVMKRGNWLDLMRQHVPESEKLYTWWSYRAKDWEAADRGRRLDHIWSSSDLGPHL
CHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCCHHHHHCCCCCCCHHH
KRIEILKEARGWDRPSDHVPVTAHFDF
HHHHHHHHHCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
GFSITTWNINSVRLRMPIVEQLVLKHRPDILCLQETKVTNELFPAAPLRAMGYDHIIIH
CCEEEEEECCEEEEECHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCHHHCCCCEEEEE
GQKGYHGVAIASRIPLTEDHRQDYCGVGDARHISAIFEHGNRRIRLHNFYVPAGGDEPDR
CCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCC
TINPKFGHKLDFIEEMKLLKANGEANTSAILVGDLNIAPLEHDVWSHKQLLKIVSHTPVE
CCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
TDGLLEVMKRGNWLDLMRQHVPESEKLYTWWSYRAKDWEAADRGRRLDHIWSSSDLGPHL
CHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCCHHHHHCCCCCCCHHH
KRIEILKEARGWDRPSDHVPVTAHFDF
HHHHHHHHHCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]