BacMap

Definition	Rhizobium leguminosarum bv. trifolii WSM2304 chromosome, complete genome.
Accession	NC_011369
Length	4,537,948

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The map label for this gene is yqhE [C]

Identifier: 209551332

GI number: 209551332

Start: 3884758

End: 3885588

Strand: Reverse

Name: yqhE [C]

Synonym: Rleg2_3761

Alternate gene names: 209551332

Gene position: 3885588-3884758 (Counterclockwise)

Preceding gene: 209551333

Following gene: 209551320

Centisome position: 85.62

GC content: 61.13

Gene sequence:

>831_bases
GTGGCCGCTCAACCCATCATCACTTTCCATGACGGACATTCCATTCCCCAGGTCGGCCTCGGCGTCTGGCAGACGCCGCA
GGAGATTGCCGCCCCGACGGTGCGTACCGCGATCGCCGCCGGCTATCGCCATATCGATACGGCATCCGGCTACGACAATG
AAGAGGGTGTCGGCGAAGGCATCCGCTCGTCCGGCCTCGACCGAAAGGACATCTTCGTCACCACCAAGCTCAAAAACACC
GATCAGGGCTACGACAATACGCTGCGCGCCTTCGACGGCAGCCTGAAAAAGCTCGGTTCCGACTATGTCGACCTTTATCT
CGTTCACTGGCCGTCGCCGCATCGCGGCCTCTATACCGAGACCTGGAAGGCTTTCGTGCGCATCCGCGAAGAGGGCCGCG
CCCGCTCGATCGGCGTGTCGAATTTCTGCCCCGAACATCTGGAGCGCATCATCGGCGAGACCGGCGTCGTTCCCGTCATC
AACCAGATCGAGCTGCATCCCGACTTCCAGCAAAAGGCGGCGCAGGAGGCACACAAGAAATTGAACATCGCCACCGAATC
CTGGAGCCCACTCGGCCAGGGCAAGTTCATCTCGAACCCTGTTATCGGTGAGATCGCCGGGAAATATCGCAAGACGCCGG
CCCAGGTCATCATCCGCTGGCATATCGACAGCGGCCTCGTCGTCATTCCGAAGTCGGTGACGCCGTCGCGCATCGAGGAA
AATTTCCAGGTGTTCGATTTCAAGCTCGATGCCGACGATATGGCGGCGATCGCCAGGCTCGACAGCGCCGGCGCCCGCAT
GGGACCGGATCCGATGACGGCGACTTTCTGA

Upstream 100 bases:

>100_bases
TTCCGGTGACCGGACGCTCCGGCTGAAATGCGTCCCGGGACGCGCAAGTGCGGGCGCAATCCATGCCGCGTCCAATTCTT
CAAACTCCCGGAGATATATT

Downstream 100 bases:

>100_bases
TCGCCGTTGCGATCATCGCTAAAGCGCGTCGCATTAGACGGGATTCATGCGACGCGCTTTAGCTCTTTGGTTTGATGCAT
GTCGTTATCCCGGAACCGCT

Product: 2,5-didehydrogluconate reductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MAAQPIITFHDGHSIPQVGLGVWQTPQEIAAPTVRTAIAAGYRHIDTASGYDNEEGVGEGIRSSGLDRKDIFVTTKLKNT
DQGYDNTLRAFDGSLKKLGSDYVDLYLVHWPSPHRGLYTETWKAFVRIREEGRARSIGVSNFCPEHLERIIGETGVVPVI
NQIELHPDFQQKAAQEAHKKLNIATESWSPLGQGKFISNPVIGEIAGKYRKTPAQVIIRWHIDSGLVVIPKSVTPSRIEE
NFQVFDFKLDADDMAAIARLDSAGARMGPDPMTATF

Sequences:

>Translated_276_residues
MAAQPIITFHDGHSIPQVGLGVWQTPQEIAAPTVRTAIAAGYRHIDTASGYDNEEGVGEGIRSSGLDRKDIFVTTKLKNT
DQGYDNTLRAFDGSLKKLGSDYVDLYLVHWPSPHRGLYTETWKAFVRIREEGRARSIGVSNFCPEHLERIIGETGVVPVI
NQIELHPDFQQKAAQEAHKKLNIATESWSPLGQGKFISNPVIGEIAGKYRKTPAQVIIRWHIDSGLVVIPKSVTPSRIEE
NFQVFDFKLDADDMAAIARLDSAGARMGPDPMTATF
>Mature_275_residues
AAQPIITFHDGHSIPQVGLGVWQTPQEIAAPTVRTAIAAGYRHIDTASGYDNEEGVGEGIRSSGLDRKDIFVTTKLKNTD
QGYDNTLRAFDGSLKKLGSDYVDLYLVHWPSPHRGLYTETWKAFVRIREEGRARSIGVSNFCPEHLERIIGETGVVPVIN
QIELHPDFQQKAAQEAHKKLNIATESWSPLGQGKFISNPVIGEIAGKYRKTPAQVIIRWHIDSGLVVIPKSVTPSRIEEN
FQVFDFKLDADDMAAIARLDSAGARMGPDPMTATF

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=282, Percent_Identity=38.2978723404255, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=36.734693877551, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI310109922, Length=276, Percent_Identity=36.9565217391304, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI310109920, Length=294, Percent_Identity=35.3741496598639, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI5453543, Length=294, Percent_Identity=35.3741496598639, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI45446745, Length=294, Percent_Identity=35.3741496598639, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI4503285, Length=294, Percent_Identity=35.3741496598639, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI93277124, Length=306, Percent_Identity=35.6209150326797, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI24497577, Length=295, Percent_Identity=32.5423728813559, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI5174391, Length=295, Percent_Identity=32.5423728813559, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI24497585, Length=293, Percent_Identity=35.4948805460751, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=34.7826086956522, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI291291012, Length=263, Percent_Identity=37.2623574144487, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI300116273, Length=282, Percent_Identity=34.7517730496454, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI4502049, Length=285, Percent_Identity=35.7894736842105, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI300116271, Length=282, Percent_Identity=32.6241134751773, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI310109924, Length=148, Percent_Identity=35.1351351351351, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI310109926, Length=126, Percent_Identity=41.2698412698413, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI207028673, Length=126, Percent_Identity=41.2698412698413, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI87082198, Length=273, Percent_Identity=52.7472527472528, Blast_Score=293, Evalue=9e-81,
Organism=Escherichia coli, GI1786400, Length=259, Percent_Identity=37.8378378378378, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1788081, Length=287, Percent_Identity=28.2229965156794, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87081735, Length=197, Percent_Identity=27.9187817258883, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI48994888, Length=200, Percent_Identity=26, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17537077, Length=284, Percent_Identity=38.7323943661972, Blast_Score=182, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17537075, Length=298, Percent_Identity=37.5838926174497, Blast_Score=181, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71998625, Length=269, Percent_Identity=32.7137546468402, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17561298, Length=269, Percent_Identity=36.4312267657993, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17564128, Length=294, Percent_Identity=33.6734693877551, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17566692, Length=278, Percent_Identity=35.6115107913669, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17550248, Length=304, Percent_Identity=33.5526315789474, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17552492, Length=265, Percent_Identity=30.9433962264151, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17538386, Length=273, Percent_Identity=31.5018315018315, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17537079, Length=293, Percent_Identity=29.0102389078498, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17562292, Length=278, Percent_Identity=30.5755395683453, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17561300, Length=273, Percent_Identity=30.03663003663, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17550246, Length=81, Percent_Identity=43.2098765432099, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324694, Length=285, Percent_Identity=38.2456140350877, Blast_Score=189, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6320576, Length=284, Percent_Identity=36.2676056338028, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322556, Length=261, Percent_Identity=33.3333333333333, Blast_Score=158, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6319625, Length=276, Percent_Identity=35.5072463768116, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6321896, Length=316, Percent_Identity=32.2784810126582, Blast_Score=139, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6320079, Length=283, Percent_Identity=32.8621908127208, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24657054, Length=279, Percent_Identity=39.0681003584229, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24662789, Length=300, Percent_Identity=34, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI20129731, Length=291, Percent_Identity=36.0824742268041, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24663317, Length=288, Percent_Identity=35.4166666666667, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI21356425, Length=296, Percent_Identity=35.472972972973, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24644950, Length=301, Percent_Identity=34.21926910299, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24662781, Length=299, Percent_Identity=39.1304347826087, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI221378297, Length=321, Percent_Identity=32.398753894081, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24662785, Length=280, Percent_Identity=40.3571428571429, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI45553081, Length=292, Percent_Identity=32.1917808219178, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI281366140, Length=252, Percent_Identity=33.7301587301587, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 30506; Mature: 30375

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQPIITFHDGHSIPQVGLGVWQTPQEIAAPTVRTAIAAGYRHIDTASGYDNEEGVGEG
CCCCCEEEECCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHCC
IRSSGLDRKDIFVTTKLKNTDQGYDNTLRAFDGSLKKLGSDYVDLYLVHWPSPHRGLYTE
HHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHH
TWKAFVRIREEGRARSIGVSNFCPEHLERIIGETGVVPVINQIELHPDFQQKAAQEAHKK
HHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCEECCCEEECCCCHHHHHHHHHHHH
LNIATESWSPLGQGKFISNPVIGEIAGKYRKTPAQVIIRWHIDSGLVVIPKSVTPSRIEE
EECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHEEEEEEECCCEEEEECCCCHHHHHC
NFQVFDFKLDADDMAAIARLDSAGARMGPDPMTATF
CCEEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAQPIITFHDGHSIPQVGLGVWQTPQEIAAPTVRTAIAAGYRHIDTASGYDNEEGVGEG
CCCCEEEECCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHCC
IRSSGLDRKDIFVTTKLKNTDQGYDNTLRAFDGSLKKLGSDYVDLYLVHWPSPHRGLYTE
HHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHH
TWKAFVRIREEGRARSIGVSNFCPEHLERIIGETGVVPVINQIELHPDFQQKAAQEAHKK
HHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCEECCCEEECCCCHHHHHHHHHHHH
LNIATESWSPLGQGKFISNPVIGEIAGKYRKTPAQVIIRWHIDSGLVVIPKSVTPSRIEE
EECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHEEEEEEECCCEEEEECCCCHHHHHC
NFQVFDFKLDADDMAAIARLDSAGARMGPDPMTATF
CCEEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA