Click here to switch to the map view.

The map label for this gene is rlmN [H]

Identifier: 209551296

GI number: 209551296

Start: 3851793

End: 3853019

Strand: Reverse

Name: rlmN [H]

Synonym: Rleg2_3724

Alternate gene names: 209551296

Gene position: 3853019-3851793 (Counterclockwise)

Preceding gene: 209551297

Following gene: 209551295

Centisome position: 84.91

GC content: 61.78

Gene sequence:

>1227_bases
ATGTCCGTCATGGATGCGATCGTTGTTAGCAAGCCGCCGGCGCACACGTCCGCCAGCTTGGAAAAGCCGTCTTTGATCGG
GCTGTCGCGCGAGGAGATGGGGGCGGCACTCCGGGAAAGGGGTGTGGCCGAGAAGCAGATCAAGATGCGCGTCGCGCAGC
TCTGGAACTGGATCTATGTGCGAGGGGTTTCCGATTTCGACCATATGACGAATGTCGCCAAGGACATGCGGGAAATGCTG
AAGCAGCATTTCACCATCGCGCGTCCTGAGATCGTCGAAGAGCAGGTCTCGAACGACGGCACGCGCAAATGGCTGCTGCG
TTTTCCCCCGCGCGGCGCCGGCCGCCCGGTCGAAATCGAAGCCGTCTATATTCCGGAAGAGGGCCGCGGCACGCTCTGCA
TCTCGAGCCAGGTCGGCTGCACGCTCACCTGTTCCTTCTGTCATACGGGCACGCAGCGTCTGGTGCGCAACCTGACGGCG
GAGGAAATTCTGTCGCAGCTCCTGCTTGCCCGCGACCGGCTTGGCGACTTCCCGGATCGCGAAGCGCCGCAGGGCACGAT
CATGCCTGCCGAAGGCCGTAAGGTCAGCAACATCGTGATGATGGGCATGGGCGAGCCGCTCTATAATTTCGACGCCGTCA
AGCAGGCATTGCTGATCGCCACGGATGGCGACGGCCTGTCGCTGTCCAGGCGCCGGGTGACGCTGTCCACCTCCGGCGTC
GTGCCGGAAATCTTCCGCACCGGCGAAGAGATCGGCGTCATGCTGGCAATCTCGCTGCATGCGGTGCGCGACGACCTGCG
CGACATTCTGGTGCCGATCAACAAGAAGTATCCGCTGAAGGAGCTGATCGAGGCGTGCAAGGCCTATCCCGGCCTTTCCA
ATGCGCGGCGCATCACCTTCGAATATGTGATGCTGAAGGATGTCAACGACAGCCTTGAAGACGCCAAGGGGCTGATCAAG
CTGCTGAAAGGCGTGCCGGCGAAGATCAACCTCATTCCGTTCAATCCCTGGCCCGGCACCAATTACCAATGTTCCGACTG
GGAGCAGATCGAGAAGTTCGCCGATTTCATCAATTCGGCCGGTTACGCCTCGCCGATCCGCACGCCGCGCGGCCGCGACA
TTCTTGCCGCCTGCGGCCAGCTGAAATCGGAGTCCGAACGCATGCGCAAGACCGAGCGCCTGGCCTTCGAGGCGATGATG
ATCGCCAATCACGGCGCCGACGACTGA

Upstream 100 bases:

>100_bases
TTTGCCGCTCTGCCGTCATGCGTCCGCAAGTCTTCCGGTTCACCCTGAAGCGTGCGGGCTCGCAAGGGGCAGGACATGTT
TGAATTCGGGATGAATGCCT

Downstream 100 bases:

>100_bases
TCAGCAAGTTTGATCCTCGCTACAATTCTCCTGCATTGATTTAAGCATGCGCATTTCCTAATAGTGCCGCCAAAATCAAT
CAGGAGGATACAATGCGCCC

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSVMDAIVVSKPPAHTSASLEKPSLIGLSREEMGAALRERGVAEKQIKMRVAQLWNWIYVRGVSDFDHMTNVAKDMREML
KQHFTIARPEIVEEQVSNDGTRKWLLRFPPRGAGRPVEIEAVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQRLVRNLTA
EEILSQLLLARDRLGDFPDREAPQGTIMPAEGRKVSNIVMMGMGEPLYNFDAVKQALLIATDGDGLSLSRRRVTLSTSGV
VPEIFRTGEEIGVMLAISLHAVRDDLRDILVPINKKYPLKELIEACKAYPGLSNARRITFEYVMLKDVNDSLEDAKGLIK
LLKGVPAKINLIPFNPWPGTNYQCSDWEQIEKFADFINSAGYASPIRTPRGRDILAACGQLKSESERMRKTERLAFEAMM
IANHGADD

Sequences:

>Translated_408_residues
MSVMDAIVVSKPPAHTSASLEKPSLIGLSREEMGAALRERGVAEKQIKMRVAQLWNWIYVRGVSDFDHMTNVAKDMREML
KQHFTIARPEIVEEQVSNDGTRKWLLRFPPRGAGRPVEIEAVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQRLVRNLTA
EEILSQLLLARDRLGDFPDREAPQGTIMPAEGRKVSNIVMMGMGEPLYNFDAVKQALLIATDGDGLSLSRRRVTLSTSGV
VPEIFRTGEEIGVMLAISLHAVRDDLRDILVPINKKYPLKELIEACKAYPGLSNARRITFEYVMLKDVNDSLEDAKGLIK
LLKGVPAKINLIPFNPWPGTNYQCSDWEQIEKFADFINSAGYASPIRTPRGRDILAACGQLKSESERMRKTERLAFEAMM
IANHGADD
>Mature_407_residues
SVMDAIVVSKPPAHTSASLEKPSLIGLSREEMGAALRERGVAEKQIKMRVAQLWNWIYVRGVSDFDHMTNVAKDMREMLK
QHFTIARPEIVEEQVSNDGTRKWLLRFPPRGAGRPVEIEAVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQRLVRNLTAE
EILSQLLLARDRLGDFPDREAPQGTIMPAEGRKVSNIVMMGMGEPLYNFDAVKQALLIATDGDGLSLSRRRVTLSTSGVV
PEIFRTGEEIGVMLAISLHAVRDDLRDILVPINKKYPLKELIEACKAYPGLSNARRITFEYVMLKDVNDSLEDAKGLIKL
LKGVPAKINLIPFNPWPGTNYQCSDWEQIEKFADFINSAGYASPIRTPRGRDILAACGQLKSESERMRKTERLAFEAMMI
ANHGADD

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=394, Percent_Identity=43.1472081218274, Blast_Score=289, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 45489; Mature: 45358

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVMDAIVVSKPPAHTSASLEKPSLIGLSREEMGAALRERGVAEKQIKMRVAQLWNWIYV
CCCHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEE
RGVSDFDHMTNVAKDMREMLKQHFTIARPEIVEEQVSNDGTRKWLLRFPPRGAGRPVEIE
ECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEE
AVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQRLVRNLTAEEILSQLLLARDRLGDFPDR
EEEECCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCC
EAPQGTIMPAEGRKVSNIVMMGMGEPLYNFDAVKQALLIATDGDGLSLSRRRVTLSTSGV
CCCCCCEECCCCCEECEEEEEECCCCCCCHHHHHHEEEEEECCCCCEEECEEEEEECCCC
VPEIFRTGEEIGVMLAISLHAVRDDLRDILVPINKKYPLKELIEACKAYPGLSNARRITF
CHHHHHCCHHHCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEEE
EYVMLKDVNDSLEDAKGLIKLLKGVPAKINLIPFNPWPGTNYQCSDWEQIEKFADFINSA
EEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
GYASPIRTPRGRDILAACGQLKSESERMRKTERLAFEAMMIANHGADD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVMDAIVVSKPPAHTSASLEKPSLIGLSREEMGAALRERGVAEKQIKMRVAQLWNWIYV
CCHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEE
RGVSDFDHMTNVAKDMREMLKQHFTIARPEIVEEQVSNDGTRKWLLRFPPRGAGRPVEIE
ECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEE
AVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQRLVRNLTAEEILSQLLLARDRLGDFPDR
EEEECCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCC
EAPQGTIMPAEGRKVSNIVMMGMGEPLYNFDAVKQALLIATDGDGLSLSRRRVTLSTSGV
CCCCCCEECCCCCEECEEEEEECCCCCCCHHHHHHEEEEEECCCCCEEECEEEEEECCCC
VPEIFRTGEEIGVMLAISLHAVRDDLRDILVPINKKYPLKELIEACKAYPGLSNARRITF
CHHHHHCCHHHCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEEE
EYVMLKDVNDSLEDAKGLIKLLKGVPAKINLIPFNPWPGTNYQCSDWEQIEKFADFINSA
EEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
GYASPIRTPRGRDILAACGQLKSESERMRKTERLAFEAMMIANHGADD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA