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The map label for this gene is argG2 [H]

Identifier: 209551290

GI number: 209551290

Start: 3845963

End: 3847186

Strand: Reverse

Name: argG2 [H]

Synonym: Rleg2_3718

Alternate gene names: 209551290

Gene position: 3847186-3845963 (Counterclockwise)

Preceding gene: 209551293

Following gene: 209551287

Centisome position: 84.78

GC content: 61.03

Gene sequence:

>1224_bases
ATGGCATCATACAAAGACGTGAAGAAAGTCGTTCTCGCCTATTCCGGCGGCCTCGACACCTCGATCATCCTGAAGTGGCT
GCAGACCGAACTCGGCGCCGAAGTCGTCACCTTCACCGCCGATCTCGGCCAGGGCGAAGAGCTGGAGCCGGCGCGCAAGA
AGGCCGAGATGCTCGGCATCAAGGAGATCTACATCGAGGATGTGCGCGAGGAATTCGTGCGCGATTTCGTCTTCCCGATG
TTCCGTGCCAATGCCGTTTACGAAGGCGTCTATCTGCTCGGCACCTCGATCGCCCGGCCGCTGATTTCCAAACATCTGAT
CGATATTGCCCGCAAGACCGGCGCCGATGCGATCGCCCATGGCGCCACCGGCAAGGGCAACGACCAGGTTCGCTTCGAAC
TCTCAGCCTATGCTTTGAACCCCGACATCAAGATCATCGCGCCGTGGCGCGACTGGTCCTTCAAGAGTCGCACCGACCTG
CTCGCCTTCGCCGAGCAGCATCAGATCCCGGTTGCCAAGGACAAGAAGGGCGAGGCGCCGTTCTCCGTCGACGCCAACCT
TCTGCATTCCTCCTCCGAGGGTAAGGTCCTGGAAGATCCGGCGCAGGAAGCGCCGGAATATGTGCATATGCGCACGATCT
CGCCTGAGGCCGCGCCCGACAAGGCAACGGTGATCAAGGTCGGCTTCCGCAAGGGCGATGCCGTCTCGATCAACGGTGTC
GAAATGTCGCCGGCGACGCTGCTCGCGACCCTCAACAATTATGGCCGCGACAACGGCATCGGCCGTCTCGACCTCGTCGA
GAACCGCTTCGTCGGCATGAAGTCGCGCGGCGTCTACGAAACGCCCGGCGGCACGATCCTGCTTGCGGCGCACCGCGCCA
TCGAATCGATCACGCTCGATCGCGGTGCCGCCCATCTCAAGGATGAGATCATGCCGCGTTACGCGGAGCTGATCTATTAC
GGCTTCTGGTTCTCGCCGGAGCGCGAGATGCTGCAGGCGCTGATCGACAAGAGCCAGGAGCATGTCGAAGGCGAAGTGAC
GCTGAAGCTCTACAAGGGCAATGTCATGGTCACCGGCCGCGAAAGCGAAAAGTCGCTCTATTCCGACAAGCTCGTGACTT
TCGAGGACGATCAGGGCGCCTACGACCAGAAGGACGCGGCCGGCTTCATCAAGCTCAATGCGCTGCGCCTGCGCACACTC
GGCAAGCGCAATCTCAAGAAGTAG

Upstream 100 bases:

>100_bases
CCGGCGGCTTAGCACTTGCCCTTGCGTCACGCGCCAATCTCGCTAAAAGTGCCGCGATACCGGGTTTTCGCCGGCCTTTT
ATGCAACGGACCTCATCATC

Downstream 100 bases:

>100_bases
TCACGGTCGCTTTCGTACCGGATATGAGCCCGCCGGCATTGCCGCGCGGGCTCTTTTTTACGCGGCGGCATCGACACGTA
GTCTGACGTCACCGCGACGC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase 2 [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MASYKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIYIEDVREEFVRDFVFPM
FRANAVYEGVYLLGTSIARPLISKHLIDIARKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTDL
LAFAEQHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPAQEAPEYVHMRTISPEAAPDKATVIKVGFRKGDAVSINGV
EMSPATLLATLNNYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLDRGAAHLKDEIMPRYAELIYY
GFWFSPEREMLQALIDKSQEHVEGEVTLKLYKGNVMVTGRESEKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTL
GKRNLKK

Sequences:

>Translated_407_residues
MASYKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIYIEDVREEFVRDFVFPM
FRANAVYEGVYLLGTSIARPLISKHLIDIARKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTDL
LAFAEQHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPAQEAPEYVHMRTISPEAAPDKATVIKVGFRKGDAVSINGV
EMSPATLLATLNNYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLDRGAAHLKDEIMPRYAELIYY
GFWFSPEREMLQALIDKSQEHVEGEVTLKLYKGNVMVTGRESEKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTL
GKRNLKK
>Mature_406_residues
ASYKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIYIEDVREEFVRDFVFPMF
RANAVYEGVYLLGTSIARPLISKHLIDIARKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTDLL
AFAEQHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPAQEAPEYVHMRTISPEAAPDKATVIKVGFRKGDAVSINGVE
MSPATLLATLNNYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLDRGAAHLKDEIMPRYAELIYYG
FWFSPEREMLQALIDKSQEHVEGEVTLKLYKGNVMVTGRESEKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTLG
KRNLKK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=398, Percent_Identity=48.9949748743719, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI16950633, Length=398, Percent_Identity=48.9949748743719, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI1789563, Length=414, Percent_Identity=27.0531400966184, Blast_Score=125, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6324514, Length=408, Percent_Identity=42.6470588235294, Blast_Score=332, Evalue=6e-92,
Organism=Drosophila melanogaster, GI21358151, Length=402, Percent_Identity=47.7611940298507, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 45231; Mature: 45100

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASYKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGI
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHH
KEIYIEDVREEFVRDFVFPMFRANAVYEGVYLLGTSIARPLISKHLIDIARKTGADAIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
GATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTDLLAFAEQHQIPVAKDKKGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPAQEAPEYVHMRTISPEAAPDKATVIKVGFRKGDAVSINGV
CCCCHHHEECCCCCCEECCHHHHCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEECCC
EMSPATLLATLNNYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLD
EECHHHHHHHHHHCCCCCCCCEEEEHHCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHHC
RGAAHLKDEIMPRYAELIYYGFWFSPEREMLQALIDKSQEHVEGEVTLKLYKGNVMVTGR
CCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHCCHHHCCCEEEEEEEECCEEEECC
ESEKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTLGKRNLKK
CHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHCCCCCCC
>Mature Secondary Structure 
ASYKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGI
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHH
KEIYIEDVREEFVRDFVFPMFRANAVYEGVYLLGTSIARPLISKHLIDIARKTGADAIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
GATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTDLLAFAEQHQIPVAKDKKGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPAQEAPEYVHMRTISPEAAPDKATVIKVGFRKGDAVSINGV
CCCCHHHEECCCCCCEECCHHHHCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEECCC
EMSPATLLATLNNYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLD
EECHHHHHHHHHHCCCCCCCCEEEEHHCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHHC
RGAAHLKDEIMPRYAELIYYGFWFSPEREMLQALIDKSQEHVEGEVTLKLYKGNVMVTGR
CCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHCCHHHCCCEEEEEEEECCEEEECC
ESEKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTLGKRNLKK
CHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA