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The map label for this gene is mdh [H]

Identifier: 209551257

GI number: 209551257

Start: 3815577

End: 3816539

Strand: Reverse

Name: mdh [H]

Synonym: Rleg2_3685

Alternate gene names: 209551257

Gene position: 3816539-3815577 (Counterclockwise)

Preceding gene: 209551258

Following gene: 209551256

Centisome position: 84.1

GC content: 63.24

Gene sequence:

>963_bases
ATGGCGCGTAACAAGATCGCACTCATTGGTTCTGGCATGATTGGTGGCACGCTGGCGCACCTCGCCGGCCTGAAGGAACT
GGGCGACATCGTCCTCTTCGACATTGCGGACGGCATTCCCCAGGGCAAGGGTCTCGATATCTCCCAGTCTTCGCCGGTCG
AAGGCTTCGACGTCAACCTGACAGGCGCCAGCGACTATTCCGCGATCGAAGGCGCTGACGTCTGCATCGTCACCGCCGGC
GTTGCCCGCAAGCCCGGCATGAGCCGCGACGATCTTCTCGGCATCAACCTCAAGGTCATGGAGCAGGTCGGCGCCGGCAT
CAAGAAATATGCCCCGAACGCTTTCGTGATCTGCATCACCAACCCGCTCGACGCTATGGTCTGGGCGCTGCAGAAGTTTT
CCGGCCTTCCGGCCAACAAGGTCGTCGGCATGGCCGGCGTTCTCGACTCCTCGCGCTTCCGTCTTTTCCTCGCCAAGGAA
TTCAACGTCTCCGTCCAGGACGTCACCGCCTTCGTGCTCGGCGGCCACGGCGACACGATGGTGCCGCTCGCCCGCTACTC
GACGGTCGGCGGCATTCCGCTCACCGACCTCGTCACCATGGGCTGGGTCACCAAGGAGCGCCTCGAAGAGATCATCCAGC
GCACGCGTGACGGCGGCGCCGAAATCGTCGGCCTGCTGAAGACCGGCTCGGCCTATTACGCGCCGGCCGCCTCGGCGATC
GAGATGGCCGAATCCTACCTCAAGGACAAGAAGCGCGTTCTGCCCTGCGCGGCTCACCTGAGCGGCCAGTACGGCGTCAA
GGACATGTATGTCGGCGTTCCCACCGTCATCGGCGCCGGCGGTGTCGAGCGCATCATCGAGATCGATCTCAACAAGACCG
AGAAGGAAGCCTTCGACAAGTCCGTCGGCGCCGTCGCCGGTCTCTGCGAAGCCTGCATCAATATCGCGCCTGCCCTCAAG
TAA

Upstream 100 bases:

>100_bases
AATGGATTGCCATTTTTAGGCTTTCGGTCTATGCGATTGCGTCATTGGGCGGTGCCTCGACGCGTCGTTCACCGAATTTG
ATCGCAAAGGAAACAGCGAA

Downstream 100 bases:

>100_bases
TTGGCGCTGAACTAATCCAAACAGGGATAGACCCATGAACATTCATGAATATCAAGCCAAGGCTCTGCTGAAGGGCTATG
GCGCGCCGGTTGCCGAGGGT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MARNKIALIGSGMIGGTLAHLAGLKELGDIVLFDIADGIPQGKGLDISQSSPVEGFDVNLTGASDYSAIEGADVCIVTAG
VARKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICITNPLDAMVWALQKFSGLPANKVVGMAGVLDSSRFRLFLAKE
FNVSVQDVTAFVLGGHGDTMVPLARYSTVGGIPLTDLVTMGWVTKERLEEIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
EMAESYLKDKKRVLPCAAHLSGQYGVKDMYVGVPTVIGAGGVERIIEIDLNKTEKEAFDKSVGAVAGLCEACINIAPALK

Sequences:

>Translated_320_residues
MARNKIALIGSGMIGGTLAHLAGLKELGDIVLFDIADGIPQGKGLDISQSSPVEGFDVNLTGASDYSAIEGADVCIVTAG
VARKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICITNPLDAMVWALQKFSGLPANKVVGMAGVLDSSRFRLFLAKE
FNVSVQDVTAFVLGGHGDTMVPLARYSTVGGIPLTDLVTMGWVTKERLEEIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
EMAESYLKDKKRVLPCAAHLSGQYGVKDMYVGVPTVIGAGGVERIIEIDLNKTEKEAFDKSVGAVAGLCEACINIAPALK
>Mature_319_residues
ARNKIALIGSGMIGGTLAHLAGLKELGDIVLFDIADGIPQGKGLDISQSSPVEGFDVNLTGASDYSAIEGADVCIVTAGV
ARKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICITNPLDAMVWALQKFSGLPANKVVGMAGVLDSSRFRLFLAKEF
NVSVQDVTAFVLGGHGDTMVPLARYSTVGGIPLTDLVTMGWVTKERLEEIIQRTRDGGAEIVGLLKTGSAYYAPAASAIE
MAESYLKDKKRVLPCAAHLSGQYGVKDMYVGVPTVIGAGGVERIIEIDLNKTEKEAFDKSVGAVAGLCEACINIAPALK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=30.4918032786885, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=30.4918032786885, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI9257228, Length=304, Percent_Identity=30.5921052631579, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4504973, Length=304, Percent_Identity=30.5921052631579, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI5031857, Length=312, Percent_Identity=29.1666666666667, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI260099723, Length=312, Percent_Identity=29.1666666666667, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI15082234, Length=303, Percent_Identity=26.7326732673267, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=28.7128712871287, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=28.7128712871287, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI260099727, Length=196, Percent_Identity=30.6122448979592, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=30.6122448979592, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI207028494, Length=195, Percent_Identity=29.7435897435897, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI21735621, Length=295, Percent_Identity=27.7966101694915, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI103472011, Length=311, Percent_Identity=21.5434083601286, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1789632, Length=312, Percent_Identity=30.7692307692308, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=246, Percent_Identity=28.0487804878049, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17554310, Length=316, Percent_Identity=30.6962025316456, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322765, Length=299, Percent_Identity=27.4247491638796, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=255, Percent_Identity=29.0196078431373, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324446, Length=255, Percent_Identity=27.843137254902, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17136226, Length=304, Percent_Identity=28.2894736842105, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI45550422, Length=302, Percent_Identity=25.8278145695364, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24647881, Length=329, Percent_Identity=29.1793313069909, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24663595, Length=265, Percent_Identity=30.5660377358491, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24663599, Length=314, Percent_Identity=28.0254777070064, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33590; Mature: 33459

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNKIALIGSGMIGGTLAHLAGLKELGDIVLFDIADGIPQGKGLDISQSSPVEGFDVNL
CCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
TGASDYSAIEGADVCIVTAGVARKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICIT
ECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPANKVVGMAGVLDSSRFRLFLAKEFNVSVQDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCHHHEEEEEECCCCCCC
VPLARYSTVGGIPLTDLVTMGWVTKERLEEIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHH
EMAESYLKDKKRVLPCAAHLSGQYGVKDMYVGVPTVIGAGGVERIIEIDLNKTEKEAFDK
HHHHHHHHHHHHHCHHHHHCCCCCCCCEEEECCCHHCCCCCCEEEEEECCCCHHHHHHHH
SVGAVAGLCEACINIAPALK
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARNKIALIGSGMIGGTLAHLAGLKELGDIVLFDIADGIPQGKGLDISQSSPVEGFDVNL
CCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
TGASDYSAIEGADVCIVTAGVARKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICIT
ECCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPANKVVGMAGVLDSSRFRLFLAKEFNVSVQDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCHHHEEEEEECCCCCCC
VPLARYSTVGGIPLTDLVTMGWVTKERLEEIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHH
EMAESYLKDKKRVLPCAAHLSGQYGVKDMYVGVPTVIGAGGVERIIEIDLNKTEKEAFDK
HHHHHHHHHHHHHCHHHHHCCCCCCCCEEEECCCHHCCCCCCEEEEEECCCCHHHHHHHH
SVGAVAGLCEACINIAPALK
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA