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The map label for this gene is sucB [H]

Identifier: 209551251

GI number: 209551251

Start: 3808147

End: 3809412

Strand: Reverse

Name: sucB [H]

Synonym: Rleg2_3679

Alternate gene names: 209551251

Gene position: 3809412-3808147 (Counterclockwise)

Preceding gene: 209551252

Following gene: 209551250

Centisome position: 83.95

GC content: 64.45

Gene sequence:

>1266_bases
ATGGCCACAGAAATCCGCGTTCCAACTCTCGGTGAATCCGTCAGCGAGGCAACCGTCGGCACCTGGTTCAAGAAGGTCGG
CGACGCCATCAAGGCCGACGAGCCGATTCTCGAGCTTGAAACCGACAAGGTGACCATCGAAGTTCCGGCACCCGCTTCCG
GCACGCTTTCGGAAATCGTTGTCGCCGCCGGCGAGACCGTCGGCCTCGGCGCGTTGCTCGGCCAGATCGCCGAAGGTGCC
GTCGCTGCTGCCGCACCGGCTGCTGCCGCACCGGCCGCCGCGCCTGCCCAGCCCGCCGCTGCTGCTCCCGCTCAGCCGGC
GCCGGTTGCCGCTGCTGCCGCATCGTCGTCGAGCGCTTCCGTTTCCACCATGCCGCCGGCACCGGCGGCTTCGAAGATGC
TTGCCGAAAGCAACCTTTCGGCCGATCAGGTCGACGGCAGCGGCAAGCGCGGCCAGGTGCTGAAGGGTGATGTCATTGCT
GCCGTCGCCAAGGGCATTTCCGCTCCGGCTGCAGCCCCCGCCGCCGCTCCTGCCGCTGCGCGCGGCCCGTCGACGGTCGA
GGATGCCTCGCGCGAAGAGCGCGTGAAGATGACGCGCCTGCGCCAGACGATCGCCAAGCGCCTCAAGGACGCGCAGAACA
CCGCCGCGATGCTTACCACCTATAATGAGGTGGACATGAAGGCGGTCATGGATCTGCGCAACAAGTACAAGGACATTTTC
GAGAAGAAACACGGCGTCAAGCTCGGCTTCATGGGCTTCTTCACGAAAGCCGTCACGCATGCGCTGAAGGAACTGCCGGC
GGTCAATGCCGAGATCGACGGCACCGACGTCATCTACAAGAACTACTGCCATGTCGGCATGGCCGTCGGCACCGACAAGG
GTCTCGTCGTTCCCGTCATCCGCGATGCCGACCAGATGTCGATCGCCGAAGTCGAGAAGGAACTCGGCCGTCTTGCCAAG
GCCGCCCGTGACGGCTCGCTCTCGATGGCCGACATGCAGGGCGGCACCTTCACCATCACCAATGGCGGCGTCTACGGCTC
GCTGATGTCGTCGCCGATCCTCAACGCGCCGCAGTCCGGCATTCTCGGCATGCACAAGATCCAGGAGCGGCCGGTGGCGA
TCGGCGGTCAGGTGGTCATCCGTCCGATGATGTATCTGGCGCTGTCCTACGATCACCGTATCGTTGACGGCAAGGAAGCG
GTCACCTTCCTCGTGCGCGTCAAGGAGAGCCTGGAAGATCCGGAACGTCTGGTTCTCGATCTCTAA

Upstream 100 bases:

>100_bases
GGTTTCCGTTTCGATATCCTGCCGATGCCCTCGATCATCTTGCGGCCGCCCGCCGTCTGAAACGCTTAAAACGAACACAC
ACGAAATCAGGATCTGAACA

Downstream 100 bases:

>100_bases
GGGAGCGCTTTCGATGCGGAACCGGATCATGCGAGCGGCGCGCCTTCTTCTCTGCGCTGCCGCCGCGCATATTCCGGTCT
CGGCAACGGCAGCCGGCTTC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MATEIRVPTLGESVSEATVGTWFKKVGDAIKADEPILELETDKVTIEVPAPASGTLSEIVVAAGETVGLGALLGQIAEGA
VAAAAPAAAAPAAAPAQPAAAAPAQPAPVAAAAASSSSASVSTMPPAPAASKMLAESNLSADQVDGSGKRGQVLKGDVIA
AVAKGISAPAAAPAAAPAAARGPSTVEDASREERVKMTRLRQTIAKRLKDAQNTAAMLTTYNEVDMKAVMDLRNKYKDIF
EKKHGVKLGFMGFFTKAVTHALKELPAVNAEIDGTDVIYKNYCHVGMAVGTDKGLVVPVIRDADQMSIAEVEKELGRLAK
AARDGSLSMADMQGGTFTITNGGVYGSLMSSPILNAPQSGILGMHKIQERPVAIGGQVVIRPMMYLALSYDHRIVDGKEA
VTFLVRVKESLEDPERLVLDL

Sequences:

>Translated_421_residues
MATEIRVPTLGESVSEATVGTWFKKVGDAIKADEPILELETDKVTIEVPAPASGTLSEIVVAAGETVGLGALLGQIAEGA
VAAAAPAAAAPAAAPAQPAAAAPAQPAPVAAAAASSSSASVSTMPPAPAASKMLAESNLSADQVDGSGKRGQVLKGDVIA
AVAKGISAPAAAPAAAPAAARGPSTVEDASREERVKMTRLRQTIAKRLKDAQNTAAMLTTYNEVDMKAVMDLRNKYKDIF
EKKHGVKLGFMGFFTKAVTHALKELPAVNAEIDGTDVIYKNYCHVGMAVGTDKGLVVPVIRDADQMSIAEVEKELGRLAK
AARDGSLSMADMQGGTFTITNGGVYGSLMSSPILNAPQSGILGMHKIQERPVAIGGQVVIRPMMYLALSYDHRIVDGKEA
VTFLVRVKESLEDPERLVLDL
>Mature_420_residues
ATEIRVPTLGESVSEATVGTWFKKVGDAIKADEPILELETDKVTIEVPAPASGTLSEIVVAAGETVGLGALLGQIAEGAV
AAAAPAAAAPAAAPAQPAAAAPAQPAPVAAAAASSSSASVSTMPPAPAASKMLAESNLSADQVDGSGKRGQVLKGDVIAA
VAKGISAPAAAPAAAPAAARGPSTVEDASREERVKMTRLRQTIAKRLKDAQNTAAMLTTYNEVDMKAVMDLRNKYKDIFE
KKHGVKLGFMGFFTKAVTHALKELPAVNAEIDGTDVIYKNYCHVGMAVGTDKGLVVPVIRDADQMSIAEVEKELGRLAKA
ARDGSLSMADMQGGTFTITNGGVYGSLMSSPILNAPQSGILGMHKIQERPVAIGGQVVIRPMMYLALSYDHRIVDGKEAV
TFLVRVKESLEDPERLVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=253, Percent_Identity=54.5454545454545, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=27.1929824561404, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI203098816, Length=456, Percent_Identity=27.1929824561404, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI110671329, Length=438, Percent_Identity=26.027397260274, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI31711992, Length=441, Percent_Identity=28.3446712018141, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.7826086956522, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786946, Length=420, Percent_Identity=50.2380952380952, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=33.6470588235294, Blast_Score=195, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI25146366, Length=416, Percent_Identity=41.1057692307692, Blast_Score=321, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17560088, Length=449, Percent_Identity=30.2895322939866, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17537937, Length=436, Percent_Identity=28.2110091743119, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=29.2604501607717, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=421, Percent_Identity=44.6555819477435, Blast_Score=357, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=25.164113785558, Blast_Score=116, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24645909, Length=239, Percent_Identity=56.0669456066946, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI18859875, Length=423, Percent_Identity=28.6052009456265, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24582497, Length=435, Percent_Identity=31.264367816092, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=30.9012875536481, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43560; Mature: 43428

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEIRVPTLGESVSEATVGTWFKKVGDAIKADEPILELETDKVTIEVPAPASGTLSEIV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCHHHHH
VAAGETVGLGALLGQIAEGAVAAAAPAAAAPAAAPAQPAAAAPAQPAPVAAAAASSSSAS
HHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
VSTMPPAPAASKMLAESNLSADQVDGSGKRGQVLKGDVIAAVAKGISAPAAAPAAAPAAA
EECCCCCCHHHHHHHHCCCCHHHCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCC
RGPSTVEDASREERVKMTRLRQTIAKRLKDAQNTAAMLTTYNEVDMKAVMDLRNKYKDIF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHH
EKKHGVKLGFMGFFTKAVTHALKELPAVNAEIDGTDVIYKNYCHVGMAVGTDKGLVVPVI
HHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEECCCCCEEEEEE
RDADQMSIAEVEKELGRLAKAARDGSLSMADMQGGTFTITNGGVYGSLMSSPILNAPQSG
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCEEHHHHCCCCCCCCCCC
ILGMHKIQERPVAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDPERLVLD
CCCHHHHHCCCCEECCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHHHCC
L
C
>Mature Secondary Structure 
ATEIRVPTLGESVSEATVGTWFKKVGDAIKADEPILELETDKVTIEVPAPASGTLSEIV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCHHHHH
VAAGETVGLGALLGQIAEGAVAAAAPAAAAPAAAPAQPAAAAPAQPAPVAAAAASSSSAS
HHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
VSTMPPAPAASKMLAESNLSADQVDGSGKRGQVLKGDVIAAVAKGISAPAAAPAAAPAAA
EECCCCCCHHHHHHHHCCCCHHHCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCC
RGPSTVEDASREERVKMTRLRQTIAKRLKDAQNTAAMLTTYNEVDMKAVMDLRNKYKDIF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHH
EKKHGVKLGFMGFFTKAVTHALKELPAVNAEIDGTDVIYKNYCHVGMAVGTDKGLVVPVI
HHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEECCCCCEEEEEE
RDADQMSIAEVEKELGRLAKAARDGSLSMADMQGGTFTITNGGVYGSLMSSPILNAPQSG
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCEEHHHHCCCCCCCCCCC
ILGMHKIQERPVAIGGQVVIRPMMYLALSYDHRIVDGKEAVTFLVRVKESLEDPERLVLD
CCCHHHHHCCCCEECCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCHHHHCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]