Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 209551229

GI number: 209551229

Start: 3784990

End: 3787206

Strand: Reverse

Name: priA [H]

Synonym: Rleg2_3657

Alternate gene names: 209551229

Gene position: 3787206-3784990 (Counterclockwise)

Preceding gene: 209551231

Following gene: 209551228

Centisome position: 83.46

GC content: 66.17

Gene sequence:

>2217_bases
ATGAGCACAGATTCGTCCAATCTCTTTGGCGCGCTTTTCGAGGCACCGCCCGCGAACCGAACGGTTCCCGTGCTGGTGCC
GATGCCGGCGCCCAAACCCTATTCCTATTCGGTGCCGGATGGCATGGCGGTCGAGCCCGGCTCGGTCGTGCAGGTGCCGC
TCGGGCCGCGGCAGGTGATCGGCGTCGTCTGGGACGGCGGCGAGGATGGCGTGGATCCGAAGAAGCTTCGGCCGATCAGC
CATGTCTTCGACTGCCCGCCGCTGACCCGGGAGATGCGCGATTTCATCGATTGGGTCGCGGCCTATACGCTCTCGCCGCC
CGGCCTCGTCGCCCGCATGGCGCTCCGGGCGCCGAACGCCTTCGAGCCGGAGCCGATGGTGGAGGGGCTGAAATTCGTCG
GCGGCGAACCGGAGCGGATGACGCCGGCGCGCGCCCGGGTGCTCGATACGGCCTCCGACGGCTTGTCATGGACACGCAGC
GGCCTGGCCCATGCGGCGGGTGTTTCGACAAGCGTCGTCGACGGGCTGATCACGCTCGGCATTTTTGAGACGATATTCCT
GCCGCCGCCGCCGGTGGTGGCATTGCCCGATCCGGAATTTACGGCCGCACGTCTCGAAGGACCGCAAAAAGAGGCCGCCG
CGGAGATCGTTTCCGATGTCCGCAAGGGCGATTTTTCCGTGTCTCTGATCGACGGGGTGACGGGATCCGGCAAGACCGAG
GTCTATTTCGAGGCGATCGCCGAGACGCTGAAACGCGGCAAGCAGGTGCTGATCCTGCTGCCGGAGATCGCGCTGACGGC
AAGTTTCCTGGAGCGTTTTCAGGACCGCTTCGGCGCAAAGCCGGCCGAATGGCATTCCGATCTCGCGCCGCGCATGCGCG
AAAAGGTCTGGCGGCAGGCGGTGACGGGCGAGGTCCGCGTCGTGGCCGGCGCCCGCTCGGCGCTTTTCCTGCCCTTCGAG
GATCTCGGCCTCATCATCGTCGACGAGGAGCATGATCCGGCCTACAAGCAGGAGGATCGCGTCTTCTATAATGCCCGCGA
CATGGCCGTGGTGCGCGGCCGCATCGGCGATTTTCCCGTCATTCTGGTGTCGGCGACGCCGTCGGTCGAAAGCCAGGTCA
ACGGGCAGAGCGGGCGCTACAGCACCGTCCACCTGCCGACGCGCTTCGGCGACGCGGCGCTGCCGGACCTGCATCTGATC
GATATGCGCCGGCATGCGCCGGAGCGCGGCGGTTTCCTGTCGCCGGTCTTGATCCGGGCGATCGGCAAGACGGTGGAGAA
GCGCGAACAGGCGCTGCTCTTTCTCAACCGCCGCGGTTATGCGCCGCTGACGCTCTGCCGCGTCTGCGGCCATCGCTTCC
AATGCCCGCAATGTTCGAGCTGGCTGGTCGAGCATCGCTTTCGCAAGCAGCTGCAATGCCATCAATGCGGCCATGCCGAG
CGCACGCCGGAGGCATGCCCGGAATGCGGCACGCTCGACCATCTGGTTGCGTGCGGCCCGGGCGTCGAACGTATCGCCGA
GGAGGTGGAACGGCATTTCCCCGAAGCGCGGACGATCGTTCTCTCCTCCGATATCATGGGCGGGGTGAAGCGGCTGCGGC
TGGAGCTGGAGGCGATCGCCAAGGGCGAGGCCGATATCGTCATCGGCACCCAGCTCGTCGCCAAGGGCCATAATTTTCCG
CTGATGACGCTGGTCGGCATCGTCGACGCCGATCTCGGCCTTGCCAATGGCGATCCGCGCGCCGCTGAACGCACCTTCCA
GCTTCTGTCGCAGGTGACCGGCCGGGCCGGGCGCACCGGCCTCAAGAGCCACGGGCTGCTGCAGACCTATCAGCCGCAGC
ATCCTGTCATGCAGGCGATCGTCTCAGGCGATTCCGACGCCTTTTATGAGCGCGAGATCACCGAACGCGAACGCGCCATA
TTGCCGCCCTTCGGCAGGCTCGCCTCGATCATCGTCTCGGCCGAAACCCGCCATGACGCCGAAAACCATGCGCGCGGCAT
GCGCAACGCGGCACCGCAGGTATCCGGCATCTCGGTGCTCGGCCCGGCGGAAGCGCCGCTGGCGCTGGTGCGCGGCCGCC
ACCGCTTCCGTCTTTTGGTACACGGCCGGCGCAACTCCGACATGCAGGGCTTTTTGCGGGCGATGCTGTCGCAATCGCCG
AAGGAGCGCGGATCGGTGCAGGTGCAGCTCGACATCGATCCGCAGAGTTTTCTCTAA

Upstream 100 bases:

>100_bases
GTAACGGCACGGACGATCTCCGAAGCCACATTCCTTGCCCGATACCCAGCCTTGTCTTTGCGGTTCCAATTGCATCGATT
TGCGGGCATGGTCTCGCCCG

Downstream 100 bases:

>100_bases
AACGCCACGCCGCCATTTCCTCCCTTTCGAGCCCATGTCATAAAACACTGATTCTTCCGGGTGATTTGGGGCTGATGCAT
GGAGTTTTATTTTCCGACCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MSTDSSNLFGALFEAPPANRTVPVLVPMPAPKPYSYSVPDGMAVEPGSVVQVPLGPRQVIGVVWDGGEDGVDPKKLRPIS
HVFDCPPLTREMRDFIDWVAAYTLSPPGLVARMALRAPNAFEPEPMVEGLKFVGGEPERMTPARARVLDTASDGLSWTRS
GLAHAAGVSTSVVDGLITLGIFETIFLPPPPVVALPDPEFTAARLEGPQKEAAAEIVSDVRKGDFSVSLIDGVTGSGKTE
VYFEAIAETLKRGKQVLILLPEIALTASFLERFQDRFGAKPAEWHSDLAPRMREKVWRQAVTGEVRVVAGARSALFLPFE
DLGLIIVDEEHDPAYKQEDRVFYNARDMAVVRGRIGDFPVILVSATPSVESQVNGQSGRYSTVHLPTRFGDAALPDLHLI
DMRRHAPERGGFLSPVLIRAIGKTVEKREQALLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRKQLQCHQCGHAE
RTPEACPECGTLDHLVACGPGVERIAEEVERHFPEARTIVLSSDIMGGVKRLRLELEAIAKGEADIVIGTQLVAKGHNFP
LMTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGLKSHGLLQTYQPQHPVMQAIVSGDSDAFYEREITERERAI
LPPFGRLASIIVSAETRHDAENHARGMRNAAPQVSGISVLGPAEAPLALVRGRHRFRLLVHGRRNSDMQGFLRAMLSQSP
KERGSVQVQLDIDPQSFL

Sequences:

>Translated_738_residues
MSTDSSNLFGALFEAPPANRTVPVLVPMPAPKPYSYSVPDGMAVEPGSVVQVPLGPRQVIGVVWDGGEDGVDPKKLRPIS
HVFDCPPLTREMRDFIDWVAAYTLSPPGLVARMALRAPNAFEPEPMVEGLKFVGGEPERMTPARARVLDTASDGLSWTRS
GLAHAAGVSTSVVDGLITLGIFETIFLPPPPVVALPDPEFTAARLEGPQKEAAAEIVSDVRKGDFSVSLIDGVTGSGKTE
VYFEAIAETLKRGKQVLILLPEIALTASFLERFQDRFGAKPAEWHSDLAPRMREKVWRQAVTGEVRVVAGARSALFLPFE
DLGLIIVDEEHDPAYKQEDRVFYNARDMAVVRGRIGDFPVILVSATPSVESQVNGQSGRYSTVHLPTRFGDAALPDLHLI
DMRRHAPERGGFLSPVLIRAIGKTVEKREQALLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRKQLQCHQCGHAE
RTPEACPECGTLDHLVACGPGVERIAEEVERHFPEARTIVLSSDIMGGVKRLRLELEAIAKGEADIVIGTQLVAKGHNFP
LMTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGLKSHGLLQTYQPQHPVMQAIVSGDSDAFYEREITERERAI
LPPFGRLASIIVSAETRHDAENHARGMRNAAPQVSGISVLGPAEAPLALVRGRHRFRLLVHGRRNSDMQGFLRAMLSQSP
KERGSVQVQLDIDPQSFL
>Mature_737_residues
STDSSNLFGALFEAPPANRTVPVLVPMPAPKPYSYSVPDGMAVEPGSVVQVPLGPRQVIGVVWDGGEDGVDPKKLRPISH
VFDCPPLTREMRDFIDWVAAYTLSPPGLVARMALRAPNAFEPEPMVEGLKFVGGEPERMTPARARVLDTASDGLSWTRSG
LAHAAGVSTSVVDGLITLGIFETIFLPPPPVVALPDPEFTAARLEGPQKEAAAEIVSDVRKGDFSVSLIDGVTGSGKTEV
YFEAIAETLKRGKQVLILLPEIALTASFLERFQDRFGAKPAEWHSDLAPRMREKVWRQAVTGEVRVVAGARSALFLPFED
LGLIIVDEEHDPAYKQEDRVFYNARDMAVVRGRIGDFPVILVSATPSVESQVNGQSGRYSTVHLPTRFGDAALPDLHLID
MRRHAPERGGFLSPVLIRAIGKTVEKREQALLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRKQLQCHQCGHAER
TPEACPECGTLDHLVACGPGVERIAEEVERHFPEARTIVLSSDIMGGVKRLRLELEAIAKGEADIVIGTQLVAKGHNFPL
MTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGLKSHGLLQTYQPQHPVMQAIVSGDSDAFYEREITERERAIL
PPFGRLASIIVSAETRHDAENHARGMRNAAPQVSGISVLGPAEAPLALVRGRHRFRLLVHGRRNSDMQGFLRAMLSQSPK
ERGSVQVQLDIDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=732, Percent_Identity=33.879781420765, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 80706; Mature: 80574

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDSSNLFGALFEAPPANRTVPVLVPMPAPKPYSYSVPDGMAVEPGSVVQVPLGPRQVI
CCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCEECCCCEEEECCCCCEEE
GVVWDGGEDGVDPKKLRPISHVFDCPPLTREMRDFIDWVAAYTLSPPGLVARMALRAPNA
EEEEECCCCCCCHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FEPEPMVEGLKFVGGEPERMTPARARVLDTASDGLSWTRSGLAHAAGVSTSVVDGLITLG
CCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
IFETIFLPPPPVVALPDPEFTAARLEGPQKEAAAEIVSDVRKGDFSVSLIDGVTGSGKTE
HHHHHCCCCCCEEECCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCH
VYFEAIAETLKRGKQVLILLPEIALTASFLERFQDRFGAKPAEWHSDLAPRMREKVWRQA
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VTGEVRVVAGARSALFLPFEDLGLIIVDEEHDPAYKQEDRVFYNARDMAVVRGRIGDFPV
HCCCEEEEECCCCEEEEEHHHCCEEEEECCCCCCCCCCCCEEECCCCHHHHHCCCCCCCE
ILVSATPSVESQVNGQSGRYSTVHLPTRFGDAALPDLHLIDMRRHAPERGGFLSPVLIRA
EEEECCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHCCCCCCCCHHHHHHHH
IGKTVEKREQALLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRKQLQCHQCGHAE
HHHHHHHHHHHEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
RTPEACPECGTLDHLVACGPGVERIAEEVERHFPEARTIVLSSDIMGGVKRLRLELEAIA
CCHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHH
KGEADIVIGTQLVAKGHNFPLMTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTG
CCCCCEEEEHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
LKSHGLLQTYQPQHPVMQAIVSGDSDAFYEREITERERAILPPFGRLASIIVSAETRHDA
CCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCH
ENHARGMRNAAPQVSGISVLGPAEAPLALVRGRHRFRLLVHGRRNSDMQGFLRAMLSQSP
HHHHHHHHHCCCCCCCEEEECCCCCCHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCC
KERGSVQVQLDIDPQSFL
HHHCCEEEEEECCHHHCC
>Mature Secondary Structure 
STDSSNLFGALFEAPPANRTVPVLVPMPAPKPYSYSVPDGMAVEPGSVVQVPLGPRQVI
CCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCEECCCCEEEECCCCCEEE
GVVWDGGEDGVDPKKLRPISHVFDCPPLTREMRDFIDWVAAYTLSPPGLVARMALRAPNA
EEEEECCCCCCCHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FEPEPMVEGLKFVGGEPERMTPARARVLDTASDGLSWTRSGLAHAAGVSTSVVDGLITLG
CCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
IFETIFLPPPPVVALPDPEFTAARLEGPQKEAAAEIVSDVRKGDFSVSLIDGVTGSGKTE
HHHHHCCCCCCEEECCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCH
VYFEAIAETLKRGKQVLILLPEIALTASFLERFQDRFGAKPAEWHSDLAPRMREKVWRQA
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VTGEVRVVAGARSALFLPFEDLGLIIVDEEHDPAYKQEDRVFYNARDMAVVRGRIGDFPV
HCCCEEEEECCCCEEEEEHHHCCEEEEECCCCCCCCCCCCEEECCCCHHHHHCCCCCCCE
ILVSATPSVESQVNGQSGRYSTVHLPTRFGDAALPDLHLIDMRRHAPERGGFLSPVLIRA
EEEECCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHCCCCCCCCHHHHHHHH
IGKTVEKREQALLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRKQLQCHQCGHAE
HHHHHHHHHHHEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
RTPEACPECGTLDHLVACGPGVERIAEEVERHFPEARTIVLSSDIMGGVKRLRLELEAIA
CCHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHH
KGEADIVIGTQLVAKGHNFPLMTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTG
CCCCCEEEEHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
LKSHGLLQTYQPQHPVMQAIVSGDSDAFYEREITERERAILPPFGRLASIIVSAETRHDA
CCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCH
ENHARGMRNAAPQVSGISVLGPAEAPLALVRGRHRFRLLVHGRRNSDMQGFLRAMLSQSP
HHHHHHHHHCCCCCCCEEEECCCCCCHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCC
KERGSVQVQLDIDPQSFL
HHHCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]