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The map label for this gene is nuoF2 [H]

Identifier: 209551211

GI number: 209551211

Start: 3768604

End: 3770160

Strand: Reverse

Name: nuoF2 [H]

Synonym: Rleg2_3637

Alternate gene names: 209551211

Gene position: 3770160-3768604 (Counterclockwise)

Preceding gene: 209551212

Following gene: 209551210

Centisome position: 83.08

GC content: 64.35

Gene sequence:

>1557_bases
ATGAGCATCAAGATTTATGTTCCGCGCGATGCCGCTGCGCTGGCGCTCGGGGCCGAAAAGGTGGCAAAGGCGATTGCCCA
GGAGATCGCCGCCCGCGGCCTCGATGTCGACATCGTGCGCAACGGCTCGCGCGGAATGTTCTGGCTGGAACCGCTTGTCG
AGGTCGAGGTCGCCGGCAAACGCGTCGGTTACGGAGCGGTGAAGGCAAGGGATATACCGGCATTGTTCGACGCCGGGATG
ATGACCGGCGGCGATCACCCGCTCTGTCTCGGGGAGGTTGAAGACCTCCCGTTCCTGAAGGAACAGGCCCGCCTGACCTT
TGCCCGCTGCGGCATCACCGATCCGCTGTCGCTTGCCGACTACGAATCGCATGGCGGTCTAGCCGGCCTTCGCCGGGCCG
TTTCGATGACATCCGCCGAAGTCGTCAAGGAAGTCACCGATTCCGGCCTGCGCGGGCGCGGCGGCGCCGGCTTTCCCACT
GGGATCAAGTGGAAGACTGTTCTCGATGCTGTCGGGGACCGCAAATATATCGTCTGCAATGCCGATGAGGGTGACAGCGG
CACCTTTGCCGACCGGATGATCATGGAGGGTGATCCCTTCGTGCTGATCGAGGGCATGGCGATAGCCGGCCTCGCCACCG
GAGCGACCAAAGGCTACGTCTACACCCGTTCGGAATATCCGCATGCCATCGCGGCAATGACCGCGGCCGTCGGCATCGCG
CGGCAGGCCGGGATACTCGGCCTATCGGTGCTCGGCTCGGGCCGCGCCTTCGATATCGAGGTGCGCACCGGCGCCGGCGC
CTATGTCTGCGGCGAGGAGACGGCTCTGCTGAACAGCCTCGAAGGCAAGCGCGGCGTCGTGCGCGCCAAGCCGCCGCTGC
CGGCGCATAAAGGGCTGTTTGACTGTCCGACCGTCATCAACAATGTGATCTCGCTTGCCTCCGTGCCTGTCATCATGGAG
AAAGGTGCCGCCTTTTACCGCGATTTCGGCATGGGCCGCTCGCGCGGCACCATTCCGCTGCAGATCGCCGGCAATGTCAG
ATATGCCGGTCTTTACGAAACAGCCTTCGGTCTTTCGCTCGGCGATATCGTCGACCGGATCGGCGGCGGCACGGCGACGG
GCCGGCCGGTCAAAGCCGTGCAGGTCGGCGGACCGCTTGGGGCCTATTTCCCGCGGGCACTGTTCGACACGCCGTTCGAC
TACGAAGCTTTCGCCGCCAAAGACGGGCTGATCGGCCATGCCGGCCTCGTCGTCTTCGACGACACGGCCGATATGCTGAA
GCAGGCGCGCTTCGCCATGGAATTCTGCGCCGTTGAAAGCTGCGGCAAGTGCACGCCCTGCCGCATCGGCTCGACGCGCG
GCGTCGAGACGGCCGACAAGATCGCCCATGGTATCGAGCCGGAGAAGAACCGGGCGCTGCTCGCCGATCTCTGCAACACG
ATGAAATTCGGCTCGCTCTGTGCGCTCGGCGGCTTCACGCCTTATCCGGTGATGAGCGCCATGACGCATTTCCCGGAGGA
TTTTTCGCCGGCGCCGATGGTGGAGGCAGCTGAGTGA

Upstream 100 bases:

>100_bases
TCGGGCTCTGCGCCTGTGCCCCATCGGCGATGCTCGACGGCGAGGTCTATGGCCGGGTCGACGATCAGCTGGCGGCGGAA
CTCGTTGCGGAGGCACGCCG

Downstream 100 bases:

>100_bases
TGGTTTTTTATTCGAAGTTGCCCCTCACCCTAACCCTCTCCCCGCTTGCGGGGGTCCGAAGGACGGGTCGAGACGCGTGG
CTCGACCCCAGGCGGTGCCG

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSIKIYVPRDAAALALGAEKVAKAIAQEIAARGLDVDIVRNGSRGMFWLEPLVEVEVAGKRVGYGAVKARDIPALFDAGM
MTGGDHPLCLGEVEDLPFLKEQARLTFARCGITDPLSLADYESHGGLAGLRRAVSMTSAEVVKEVTDSGLRGRGGAGFPT
GIKWKTVLDAVGDRKYIVCNADEGDSGTFADRMIMEGDPFVLIEGMAIAGLATGATKGYVYTRSEYPHAIAAMTAAVGIA
RQAGILGLSVLGSGRAFDIEVRTGAGAYVCGEETALLNSLEGKRGVVRAKPPLPAHKGLFDCPTVINNVISLASVPVIME
KGAAFYRDFGMGRSRGTIPLQIAGNVRYAGLYETAFGLSLGDIVDRIGGGTATGRPVKAVQVGGPLGAYFPRALFDTPFD
YEAFAAKDGLIGHAGLVVFDDTADMLKQARFAMEFCAVESCGKCTPCRIGSTRGVETADKIAHGIEPEKNRALLADLCNT
MKFGSLCALGGFTPYPVMSAMTHFPEDFSPAPMVEAAE

Sequences:

>Translated_518_residues
MSIKIYVPRDAAALALGAEKVAKAIAQEIAARGLDVDIVRNGSRGMFWLEPLVEVEVAGKRVGYGAVKARDIPALFDAGM
MTGGDHPLCLGEVEDLPFLKEQARLTFARCGITDPLSLADYESHGGLAGLRRAVSMTSAEVVKEVTDSGLRGRGGAGFPT
GIKWKTVLDAVGDRKYIVCNADEGDSGTFADRMIMEGDPFVLIEGMAIAGLATGATKGYVYTRSEYPHAIAAMTAAVGIA
RQAGILGLSVLGSGRAFDIEVRTGAGAYVCGEETALLNSLEGKRGVVRAKPPLPAHKGLFDCPTVINNVISLASVPVIME
KGAAFYRDFGMGRSRGTIPLQIAGNVRYAGLYETAFGLSLGDIVDRIGGGTATGRPVKAVQVGGPLGAYFPRALFDTPFD
YEAFAAKDGLIGHAGLVVFDDTADMLKQARFAMEFCAVESCGKCTPCRIGSTRGVETADKIAHGIEPEKNRALLADLCNT
MKFGSLCALGGFTPYPVMSAMTHFPEDFSPAPMVEAAE
>Mature_517_residues
SIKIYVPRDAAALALGAEKVAKAIAQEIAARGLDVDIVRNGSRGMFWLEPLVEVEVAGKRVGYGAVKARDIPALFDAGMM
TGGDHPLCLGEVEDLPFLKEQARLTFARCGITDPLSLADYESHGGLAGLRRAVSMTSAEVVKEVTDSGLRGRGGAGFPTG
IKWKTVLDAVGDRKYIVCNADEGDSGTFADRMIMEGDPFVLIEGMAIAGLATGATKGYVYTRSEYPHAIAAMTAAVGIAR
QAGILGLSVLGSGRAFDIEVRTGAGAYVCGEETALLNSLEGKRGVVRAKPPLPAHKGLFDCPTVINNVISLASVPVIMEK
GAAFYRDFGMGRSRGTIPLQIAGNVRYAGLYETAFGLSLGDIVDRIGGGTATGRPVKAVQVGGPLGAYFPRALFDTPFDY
EAFAAKDGLIGHAGLVVFDDTADMLKQARFAMEFCAVESCGKCTPCRIGSTRGVETADKIAHGIEPEKNRALLADLCNTM
KFGSLCALGGFTPYPVMSAMTHFPEDFSPAPMVEAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=370, Percent_Identity=37.8378378378378, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI260656005, Length=370, Percent_Identity=37.8378378378378, Blast_Score=247, Evalue=2e-65,
Organism=Escherichia coli, GI1788620, Length=412, Percent_Identity=37.621359223301, Blast_Score=282, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17531727, Length=449, Percent_Identity=36.3028953229399, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI19920794, Length=371, Percent_Identity=38.8140161725067, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI45550452, Length=371, Percent_Identity=36.6576819407008, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24653819, Length=315, Percent_Identity=32.3809523809524, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24653817, Length=279, Percent_Identity=30.8243727598566, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54524; Mature: 54393

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKIYVPRDAAALALGAEKVAKAIAQEIAARGLDVDIVRNGSRGMFWLEPLVEVEVAGK
CEEEEEECCCHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECHHEEEEECCC
RVGYGAVKARDIPALFDAGMMTGGDHPLCLGEVEDLPFLKEQARLTFARCGITDPLSLAD
CCCCCCEECCCCCHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHEEHHHCCCCCCCCCCC
YESHGGLAGLRRAVSMTSAEVVKEVTDSGLRGRGGAGFPTGIKWKTVLDAVGDRKYIVCN
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEE
ADEGDSGTFADRMIMEGDPFVLIEGMAIAGLATGATKGYVYTRSEYPHAIAAMTAAVGIA
CCCCCCCCHHHHEEECCCCEEEEECCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHH
RQAGILGLSVLGSGRAFDIEVRTGAGAYVCGEETALLNSLEGKRGVVRAKPPLPAHKGLF
HHCCEEEEEEECCCCEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCCCCCCCC
DCPTVINNVISLASVPVIMEKGAAFYRDFGMGRSRGTIPLQIAGNVRYAGLYETAFGLSL
CCHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCCCCCEEEEEECCEEEEEEHHHHHCCCH
GDIVDRIGGGTATGRPVKAVQVGGPLGAYFPRALFDTPFDYEAFAAKDGLIGHAGLVVFD
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHCCCCCEECCCEEEEC
DTADMLKQARFAMEFCAVESCGKCTPCRIGSTRGVETADKIAHGIEPEKNRALLADLCNT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHH
MKFGSLCALGGFTPYPVMSAMTHFPEDFSPAPMVEAAE
HHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SIKIYVPRDAAALALGAEKVAKAIAQEIAARGLDVDIVRNGSRGMFWLEPLVEVEVAGK
EEEEEECCCHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECHHEEEEECCC
RVGYGAVKARDIPALFDAGMMTGGDHPLCLGEVEDLPFLKEQARLTFARCGITDPLSLAD
CCCCCCEECCCCCHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHEEHHHCCCCCCCCCCC
YESHGGLAGLRRAVSMTSAEVVKEVTDSGLRGRGGAGFPTGIKWKTVLDAVGDRKYIVCN
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEE
ADEGDSGTFADRMIMEGDPFVLIEGMAIAGLATGATKGYVYTRSEYPHAIAAMTAAVGIA
CCCCCCCCHHHHEEECCCCEEEEECCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHH
RQAGILGLSVLGSGRAFDIEVRTGAGAYVCGEETALLNSLEGKRGVVRAKPPLPAHKGLF
HHCCEEEEEEECCCCEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCCCCCCCC
DCPTVINNVISLASVPVIMEKGAAFYRDFGMGRSRGTIPLQIAGNVRYAGLYETAFGLSL
CCHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCCCCCEEEEEECCEEEEEEHHHHHCCCH
GDIVDRIGGGTATGRPVKAVQVGGPLGAYFPRALFDTPFDYEAFAAKDGLIGHAGLVVFD
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHCCCCCEECCCEEEEC
DTADMLKQARFAMEFCAVESCGKCTPCRIGSTRGVETADKIAHGIEPEKNRALLADLCNT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHH
MKFGSLCALGGFTPYPVMSAMTHFPEDFSPAPMVEAAE
HHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]