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The map label for this gene is yafV [C]

Identifier: 209551106

GI number: 209551106

Start: 3659848

End: 3660705

Strand: Reverse

Name: yafV [C]

Synonym: Rleg2_3530

Alternate gene names: 209551106

Gene position: 3660705-3659848 (Counterclockwise)

Preceding gene: 209551107

Following gene: 209551105

Centisome position: 80.67

GC content: 65.62

Gene sequence:

>858_bases
ATGAGCTTCAAGGCCGCCGCCGTTCAGATGTGCTCCGGGGTCGATCCGGAGAAGAATGCTGCCGCCATGGCGCGGTTGGT
CCGCGAGGCAGCCGCCCAGGGCGCGACCTATGTGCAGACGCCTGAAATGACCGGCATGCTGCAGCGCGACCGCGCGGCGT
CGCGTGCGGTGCTCGCAGACGAGACGCATGATATCATCGTGAGGACCGGCTCGGAACTCGCCCGGGAGCTCGGCATCCAC
ATGCATGTCGGCTCGACGGCAATTGCGCTCGCGGACGGCAAGATCGCCAATCGCGGTTTCCTGTTCGGCCCCGACGGCCG
GATCCTCAATCGATACGACAAGATCCACATGTTCGACGTCGACCTCGACAATGGCGAGAGCTGGCGTGAAAGTGCCGCCT
ATACCGCCGGCTCGGAGGCGCGGGTCCTGTCGCTGCCCTTTGCCGAAATGGGCTTTGCGATCTGCTACGACGTGCGTTTT
CCCGCGCTCTTCCGCGCCCAGGCGGTTGCCGGTGCCGAAGTGATGACGGTTCCGGCTGCCTTCACCAAGCAGACCGGCGA
GGCGCATTGGGAGATATTGCTCAGGGCACGCGCGATCGAGAACGGTGTCTTCGTCATCGCCGCGGCGCAGGCCGGCCGGC
ACGAGGACGGGCGCGAGACTTTCGGCCATTCGATGATCATCGATCCCTGGGGCACGGTGCTGGCCTCGGCCGGCGCCACC
GGCGAGGCTGTTATTGTCGCCGAGATCGATCCCGCCGCGGTCAAGGCTGCGCATGACAAGATCCCGAACCTCAGGAACGG
CCGGGAATTCTCGGTCGAGAAGATTGCGGGGGCAGTCGCTGGAGGCGTCGCCGCTTGA

Upstream 100 bases:

>100_bases
ATCTTCATCGGCGCCGATCATGTCGGCGGCTGCGACGATCTCTTTGCGCTCGATCGGGCCGGTAAGCTCGATCCGATGCT
GGCGGCCTGAGGACGAACCG

Downstream 100 bases:

>100_bases
TCCGCTATTCCCTCACTTGCGACAATGCCCATGAATTCGAAGGCTGGTTTTCCGAAAGCGCCGATTTCGACCGTCAGGTC
GCGACCGGTTTCCTGACCTG

Product: nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSFKAAAVQMCSGVDPEKNAAAMARLVREAAAQGATYVQTPEMTGMLQRDRAASRAVLADETHDIIVRTGSELARELGIH
MHVGSTAIALADGKIANRGFLFGPDGRILNRYDKIHMFDVDLDNGESWRESAAYTAGSEARVLSLPFAEMGFAICYDVRF
PALFRAQAVAGAEVMTVPAAFTKQTGEAHWEILLRARAIENGVFVIAAAQAGRHEDGRETFGHSMIIDPWGTVLASAGAT
GEAVIVAEIDPAAVKAAHDKIPNLRNGREFSVEKIAGAVAGGVAA

Sequences:

>Translated_285_residues
MSFKAAAVQMCSGVDPEKNAAAMARLVREAAAQGATYVQTPEMTGMLQRDRAASRAVLADETHDIIVRTGSELARELGIH
MHVGSTAIALADGKIANRGFLFGPDGRILNRYDKIHMFDVDLDNGESWRESAAYTAGSEARVLSLPFAEMGFAICYDVRF
PALFRAQAVAGAEVMTVPAAFTKQTGEAHWEILLRARAIENGVFVIAAAQAGRHEDGRETFGHSMIIDPWGTVLASAGAT
GEAVIVAEIDPAAVKAAHDKIPNLRNGREFSVEKIAGAVAGGVAA
>Mature_284_residues
SFKAAAVQMCSGVDPEKNAAAMARLVREAAAQGATYVQTPEMTGMLQRDRAASRAVLADETHDIIVRTGSELARELGIHM
HVGSTAIALADGKIANRGFLFGPDGRILNRYDKIHMFDVDLDNGESWRESAAYTAGSEARVLSLPFAEMGFAICYDVRFP
ALFRAQAVAGAEVMTVPAAFTKQTGEAHWEILLRARAIENGVFVIAAAQAGRHEDGRETFGHSMIIDPWGTVLASAGATG
EAVIVAEIDPAAVKAAHDKIPNLRNGREFSVEKIAGAVAGGVAA

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=268, Percent_Identity=38.0597014925373, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI297632350, Length=268, Percent_Identity=38.0597014925373, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI5031947, Length=268, Percent_Identity=38.0597014925373, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI9910460, Length=271, Percent_Identity=34.6863468634686, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI297632346, Length=191, Percent_Identity=35.6020942408377, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786412, Length=189, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556280, Length=270, Percent_Identity=34.8148148148148, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322335, Length=296, Percent_Identity=31.0810810810811, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323383, Length=208, Percent_Identity=36.5384615384615, Blast_Score=149, Evalue=7e-37,
Organism=Drosophila melanogaster, GI17933642, Length=263, Percent_Identity=36.5019011406844, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21355835, Length=193, Percent_Identity=35.2331606217617, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30235; Mature: 30103

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFKAAAVQMCSGVDPEKNAAAMARLVREAAAQGATYVQTPEMTGMLQRDRAASRAVLAD
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCEEECC
ETHDIIVRTGSELARELGIHMHVGSTAIALADGKIANRGFLFGPDGRILNRYDKIHMFDV
CCCEEEEECHHHHHHHCCCEEEECCEEEEEECCCCCCCCEEECCCCHHCCCCCEEEEEEE
DLDNGESWRESAAYTAGSEARVLSLPFAEMGFAICYDVRFPALFRAQAVAGAEVMTVPAA
ECCCCCHHHHHHCCCCCCCCEEEECCHHHHCEEEEEECCCCHHHHHHHHCCCEEEEECHH
FTKQTGEAHWEILLRARAIENGVFVIAAAQAGRHEDGRETFGHSMIIDPWGTVLASAGAT
HHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCCEEECCHHHHHHCCCCC
GEAVIVAEIDPAAVKAAHDKIPNLRNGREFSVEKIAGAVAGGVAA
CCEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SFKAAAVQMCSGVDPEKNAAAMARLVREAAAQGATYVQTPEMTGMLQRDRAASRAVLAD
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCEEECC
ETHDIIVRTGSELARELGIHMHVGSTAIALADGKIANRGFLFGPDGRILNRYDKIHMFDV
CCCEEEEECHHHHHHHCCCEEEECCEEEEEECCCCCCCCEEECCCCHHCCCCCEEEEEEE
DLDNGESWRESAAYTAGSEARVLSLPFAEMGFAICYDVRFPALFRAQAVAGAEVMTVPAA
ECCCCCHHHHHHCCCCCCCCEEEECCHHHHCEEEEEECCCCHHHHHHHHCCCEEEEECHH
FTKQTGEAHWEILLRARAIENGVFVIAAAQAGRHEDGRETFGHSMIIDPWGTVLASAGAT
HHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCCEEECCHHHHHHCCCCC
GEAVIVAEIDPAAVKAAHDKIPNLRNGREFSVEKIAGAVAGGVAA
CCEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]