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The map label for this gene is hemK [H]

Identifier: 209551099

GI number: 209551099

Start: 3652181

End: 3653041

Strand: Reverse

Name: hemK [H]

Synonym: Rleg2_3523

Alternate gene names: 209551099

Gene position: 3653041-3652181 (Counterclockwise)

Preceding gene: 209551100

Following gene: 209551098

Centisome position: 80.5

GC content: 60.51

Gene sequence:

>861_bases
ATGAGTTCGACGGTCGCCGATACGCTCGCCGAAGCGCGCCGCCGCTTCACGGAGGCGGGTATCGCCGACCCGGCGACCGA
TGCGCGCTTGCTCGTCGCCGGCCTTCTGAAACTATCGCCGACCGAGCTTTTGACGCGATCGGCCGAGAGGCTTTCGCCTG
AGCAGGCGGAGGTGTTGTCCAAGGCGGTCGAGCGGCGCCTCGGCCATGAGCCGGTGCACCGCATTCTCGGCGAGCGGGAA
TTCTACGGCCTGCCGCTTGCCCTCTCGGCGGAAACGCTGGAGCCGCGGCCGGATACGGAAATCCTGGTCGATACGGTGCT
TGCCTGTCTCAAGGACCTTGCAAAGGAGCAGAGCCATCTCCATATCCTTGACGTGGGAACCGGGACCGGGGCGATATGCC
TTGCGCTTTTGAGCGAATGTCCTGATGCGTCGGGTGTCGGGAGCGACATATCGGCGGATGCCCTTCGCACAGCAAGATCG
AATGCAGAAAGAAACGGGTTGCAGGATCGCTTTCAGGCCGTGCAGTCGAGATGGTTCGAGAGCATCCAGGGTTCGTTTCA
CGCGATTGTCTCAAATCCGCCCTATATTGCTTCCAATGTCATTCACGATCTCGCTCCCGAAGTGACGAAATTTGATCCGG
TCGCGGCACTGGATGGCGGCCCGGATGGGCTTGACGCCTACCGCGCCATCGCCAAGGATGCGGCAAGGTTCATGAGGCCC
GATGGGGTCGTCGGGCTGGAAATCGGTTACGATCAGCGCAACGATGTGACGGCGATCTTTGAAGCGAAAGGCTTCAAGTG
CCTCAAATCCGTAAAAGATTATGGTCAGAACGATAGGGTGCTCGTGTTCGCGCTCGCGTGA

Upstream 100 bases:

>100_bases
ATAAGCTCGACCGGATGATGGAAGGTGAGATCGAGGAAGTCGTCGATGCGCTGATGGCCGACTACCAGGCAAGCCAGCTG
GCGCAGCTCGGCGAGCAGCA

Downstream 100 bases:

>100_bases
CAGGCGATAATCGCTGCAGTGAACGGGACATTATTGTTATTGCGAAGAAAAGGCTTGGATTTCCCAGGGAAGCAATATAG
GTTGCGGTCACGTGTTGGGC

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: M.MloHemKP [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSSTVADTLAEARRRFTEAGIADPATDARLLVAGLLKLSPTELLTRSAERLSPEQAEVLSKAVERRLGHEPVHRILGERE
FYGLPLALSAETLEPRPDTEILVDTVLACLKDLAKEQSHLHILDVGTGTGAICLALLSECPDASGVGSDISADALRTARS
NAERNGLQDRFQAVQSRWFESIQGSFHAIVSNPPYIASNVIHDLAPEVTKFDPVAALDGGPDGLDAYRAIAKDAARFMRP
DGVVGLEIGYDQRNDVTAIFEAKGFKCLKSVKDYGQNDRVLVFALA

Sequences:

>Translated_286_residues
MSSTVADTLAEARRRFTEAGIADPATDARLLVAGLLKLSPTELLTRSAERLSPEQAEVLSKAVERRLGHEPVHRILGERE
FYGLPLALSAETLEPRPDTEILVDTVLACLKDLAKEQSHLHILDVGTGTGAICLALLSECPDASGVGSDISADALRTARS
NAERNGLQDRFQAVQSRWFESIQGSFHAIVSNPPYIASNVIHDLAPEVTKFDPVAALDGGPDGLDAYRAIAKDAARFMRP
DGVVGLEIGYDQRNDVTAIFEAKGFKCLKSVKDYGQNDRVLVFALA
>Mature_285_residues
SSTVADTLAEARRRFTEAGIADPATDARLLVAGLLKLSPTELLTRSAERLSPEQAEVLSKAVERRLGHEPVHRILGEREF
YGLPLALSAETLEPRPDTEILVDTVLACLKDLAKEQSHLHILDVGTGTGAICLALLSECPDASGVGSDISADALRTARSN
AERNGLQDRFQAVQSRWFESIQGSFHAIVSNPPYIASNVIHDLAPEVTKFDPVAALDGGPDGLDAYRAIAKDAARFMRPD
GVVGLEIGYDQRNDVTAIFEAKGFKCLKSVKDYGQNDRVLVFALA

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=263, Percent_Identity=30.0380228136882, Blast_Score=108, Evalue=4e-24,
Organism=Escherichia coli, GI1787463, Length=258, Percent_Identity=36.4341085271318, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI87082085, Length=257, Percent_Identity=30.7392996108949, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24582226, Length=280, Percent_Identity=29.6428571428571, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30877; Mature: 30746

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTVADTLAEARRRFTEAGIADPATDARLLVAGLLKLSPTELLTRSAERLSPEQAEVLS
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
KAVERRLGHEPVHRILGEREFYGLPLALSAETLEPRPDTEILVDTVLACLKDLAKEQSHL
HHHHHHHCHHHHHHHHCCCHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
HILDVGTGTGAICLALLSECPDASGVGSDISADALRTARSNAERNGLQDRFQAVQSRWFE
EEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SIQGSFHAIVSNPPYIASNVIHDLAPEVTKFDPVAALDGGPDGLDAYRAIAKDAARFMRP
HHCCCCEEEECCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DGVVGLEIGYDQRNDVTAIFEAKGFKCLKSVKDYGQNDRVLVFALA
CCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
SSTVADTLAEARRRFTEAGIADPATDARLLVAGLLKLSPTELLTRSAERLSPEQAEVLS
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
KAVERRLGHEPVHRILGEREFYGLPLALSAETLEPRPDTEILVDTVLACLKDLAKEQSHL
HHHHHHHCHHHHHHHHCCCHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
HILDVGTGTGAICLALLSECPDASGVGSDISADALRTARSNAERNGLQDRFQAVQSRWFE
EEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SIQGSFHAIVSNPPYIASNVIHDLAPEVTKFDPVAALDGGPDGLDAYRAIAKDAARFMRP
HHCCCCEEEECCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DGVVGLEIGYDQRNDVTAIFEAKGFKCLKSVKDYGQNDRVLVFALA
CCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]