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The map label for this gene is rpoE [H]

Identifier: 209551010

GI number: 209551010

Start: 3549635

End: 3550387

Strand: Direct

Name: rpoE [H]

Synonym: Rleg2_3434

Alternate gene names: 209551010

Gene position: 3549635-3550387 (Clockwise)

Preceding gene: 209551005

Following gene: 209551011

Centisome position: 78.22

GC content: 60.03

Gene sequence:

>753_bases
ATGATGACAAACGTCCAGATCAACTATCCCTTCGGATCGACCGATTGCATGCTGCTCGTCCGGCATGCGTCGGAAGCCCG
TATTCCACCATCACCGCTGGCCGTACTTCGCGGCGGTGCGGCGTGGCTGTTCAATCTCATGGTCTCGGGAAAAGCGCGCA
GCGATCGGGTCGCTTCGGCAGCCGAGACGAGCGTGCAGCATGAATTCATGCAGCCCTTTCAGGACATCATCGTTCCCCAT
CTTGACGCGGCTTATAGTTTTGCCCGCTTCCTCAGCCGCGACGCCGATGCCGCGCAGGATATTGTCCAGGAAGCGTTTCT
GCGCGCCTATCGCAATTTCGAAACCTATCGTGGCGGCGACCCGCGCGCCTGGCTCTTTGCCATTGTCAGGAACTGCTGTC
ATGTCTGGCGCCAGCAGGATCGCCGCAAAGCGCGGTTCGAGCAGTCTTTAGGCAATGACGGCTATGCTGACCTCGACGAA
GGCGGCGAATACCAGATCGCCTCGGAGGAGGATTCGCCGGAGATGGCGACGATCCGACGAAGCGAACAGCAGCGCGTACG
CGCCGTCATCAGCCGGCTTCCGGAGGCGATGAGGGAAATCCTTGTCTTGCGCGAGCTTGAAGATCTTTCCTACCGACAGA
TCGCCGAGATCATCGATGCGCCGATCGGCACGGTCATGTCGCGGCTTGCCAGGGCGCGGCGTGAATTTGGCGAAGCCTGG
GATGCATGCAGCAAAAGAGAGACGGCAGGATGA

Upstream 100 bases:

>100_bases
ATGGAATAAAACCGCTTGCCGATCGGTCTAGATCGCAAGGGGTCGCATTGCATGCCTGCGGGCCCCACGCCTGAACTTGG
CTGCTGTTGAGGCAGGGAGA

Downstream 100 bases:

>100_bases
GTGAAGCCCAGGAGAAAGGCTGCCCGGAATGGCGCATCATGCTGCACGGCTTTGTCGATAACGAACTCGATTCCGTCCAC
ACGGCGCAATTCGAGGATCA

Product: ECF subfamily RNA polymerase sigma-24 subunit

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MMTNVQINYPFGSTDCMLLVRHASEARIPPSPLAVLRGGAAWLFNLMVSGKARSDRVASAAETSVQHEFMQPFQDIIVPH
LDAAYSFARFLSRDADAAQDIVQEAFLRAYRNFETYRGGDPRAWLFAIVRNCCHVWRQQDRRKARFEQSLGNDGYADLDE
GGEYQIASEEDSPEMATIRRSEQQRVRAVISRLPEAMREILVLRELEDLSYRQIAEIIDAPIGTVMSRLARARREFGEAW
DACSKRETAG

Sequences:

>Translated_250_residues
MMTNVQINYPFGSTDCMLLVRHASEARIPPSPLAVLRGGAAWLFNLMVSGKARSDRVASAAETSVQHEFMQPFQDIIVPH
LDAAYSFARFLSRDADAAQDIVQEAFLRAYRNFETYRGGDPRAWLFAIVRNCCHVWRQQDRRKARFEQSLGNDGYADLDE
GGEYQIASEEDSPEMATIRRSEQQRVRAVISRLPEAMREILVLRELEDLSYRQIAEIIDAPIGTVMSRLARARREFGEAW
DACSKRETAG
>Mature_250_residues
MMTNVQINYPFGSTDCMLLVRHASEARIPPSPLAVLRGGAAWLFNLMVSGKARSDRVASAAETSVQHEFMQPFQDIIVPH
LDAAYSFARFLSRDADAAQDIVQEAFLRAYRNFETYRGGDPRAWLFAIVRNCCHVWRQQDRRKARFEQSLGNDGYADLDE
GGEYQIASEEDSPEMATIRRSEQQRVRAVISRLPEAMREILVLRELEDLSYRQIAEIIDAPIGTVMSRLARARREFGEAW
DACSKRETAG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=136, Percent_Identity=36.7647058823529, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 28407; Mature: 28407

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTNVQINYPFGSTDCMLLVRHASEARIPPSPLAVLRGGAAWLFNLMVSGKARSDRVASA
CCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHH
AETSVQHEFMQPFQDIIVPHLDAAYSFARFLSRDADAAQDIVQEAFLRAYRNFETYRGGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PRAWLFAIVRNCCHVWRQQDRRKARFEQSLGNDGYADLDEGGEYQIASEEDSPEMATIRR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHH
SEQQRVRAVISRLPEAMREILVLRELEDLSYRQIAEIIDAPIGTVMSRLARARREFGEAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DACSKRETAG
HHHHCCCCCC
>Mature Secondary Structure
MMTNVQINYPFGSTDCMLLVRHASEARIPPSPLAVLRGGAAWLFNLMVSGKARSDRVASA
CCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHH
AETSVQHEFMQPFQDIIVPHLDAAYSFARFLSRDADAAQDIVQEAFLRAYRNFETYRGGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PRAWLFAIVRNCCHVWRQQDRRKARFEQSLGNDGYADLDEGGEYQIASEEDSPEMATIRR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHH
SEQQRVRAVISRLPEAMREILVLRELEDLSYRQIAEIIDAPIGTVMSRLARARREFGEAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DACSKRETAG
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]