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The map label for this gene is lysS [H]

Identifier: 209550996

GI number: 209550996

Start: 3536620

End: 3538113

Strand: Direct

Name: lysS [H]

Synonym: Rleg2_3420

Alternate gene names: 209550996

Gene position: 3536620-3538113 (Clockwise)

Preceding gene: 209550995

Following gene: 209550998

Centisome position: 77.93

GC content: 61.11

Gene sequence:

>1494_bases
ATGAACGACAAGACCGAAAACACCCTTTCCTCCGACGTGACTGAGGTGCGCGCCCAGAAGCTGAAGCTGCTGCGCGAGCA
GGTCGGCGACGTCTATCCGGCGCATTTCCACCGGACGCTGACCAATGCCGAGCTCGGCGCCAAGTACGAATCTCTGGAAT
CCGACGTCGAGACGCAGGATGTCGTCACCGTCGCCGGCCGCGTCTATTCCTCGCGCAACTCCGGCATGTTCATGGATATC
CATGACGCTTCCGGCAAGATCCAGATCTTCAGCCACAAGGACACGACGCCGGAAGAGGCCCGCGCTTTGCTGCCGATGAT
CGACATCGGCGACATCATCGGCGTGACCGGCATCGTGCGCCGCACCAAGCGCGGCGAGCTGACGATCAACGCCCAGAAGA
TCGAGATGCTGACCAAGTCGCTGCTGCCGATGCCCGAGAAGTGGCACGGCCTCTCCGACATCGAGCTGCGGTATCGCAAG
CGCCATCTCGACATCATGACCAACGAGGATTCGAAGCTTCGCTTCCAGCAGCGCTCGAAGATCGTCTCCGGCATCCGCCG
CTTCATGGAGAATGACGGCTTCATGGAAGTCGAGACGCCGATGCTGCAATCGATCTATGGCGGCGCCACCGCCGAGCCGT
TCAAGACGCATCACAACACGCTGAAGCTCGACATGTATCTGCGCATCGCGCCGGAACTGTTCCTGAAGCGCACGCTGGTA
TCGGGCCTCACCGACAAGGTCTTCGAGATCAACCGGAACTTCCGCAACGAAGGCGTCTCCACCCGGCACAATCCCGAGTT
CACGATGATGGAGTGTTACTGGGCCTATGCCGATTACGAGGACATGATGGACCTCGTCGAGCGGCTGTTCGAAACCCTGG
CGCTCTCCATCCATGGCACCACCGAATTCGATTTCGGCGACAAGCAGCTGTCCTTCAAGGGTCCGTTCAAGCGCGTACCG
ATGCCTGACGCCGTCAAGCAAGTGACCGGCATCGATTTCCTTGCGATCAAGACCGACGAGGAAGCACGCACTGCTGCCAA
GGCTGCCGGCTTCGCGGTCGAGAAGGACTGGACCTGGGGCGAATGCCTTGCCTTCATCTTCGAGGAAAAGGTTGAATCCA
CACTGATCCAGCCGTCTCACGTCACGCATTTCCCGAAGGACATCTCACCCTTCGCCAAGGAAGTGCCGGGCGAACCGCGC
CTCGTCGAGCGCTTCGAGACCTATTGCAACGCCTGGGAACTCGGCAACGCCTTCTCCGAGCTCAACGATCCCGAAGAGCA
GCGCCGCCGCATGGTCGAGCAGCTCGAACAGGCGCATGCCCGCGGCGAAAAGGAAAAGCAGTTGGACGAGGAATTCCTCG
ACGCGATCGACCAGGGCATGCCGCCGGCCGGCGGCCTCGGCATCGGCGTCGACCGCCTGATCATGCTTTTGACCAACGCC
CCGTCGATCCGCGACGTCATCCTCTTCCCGGCCCGCCGCAACAAGGCCGACTGA

Upstream 100 bases:

>100_bases
ACCCTCCCCACAAGGAGAGGGTTAGCGTCGAGCCAGTCGCCACGCTTCGGGTAAACGAACGTTGGTTTAGAATTCATGCC
GCATCGATGAGGCAAAAAAC

Downstream 100 bases:

>100_bases
CGGGAATAAACGTCAAAACCAAATAAGCGGAGTGCCGACCGGCGCTCTGCTTTTTTATGCCGTTCGTTCAGTGGGTCTTT
GGCGCTTCTGCCGGCGGTTC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNDKTENTLSSDVTEVRAQKLKLLREQVGDVYPAHFHRTLTNAELGAKYESLESDVETQDVVTVAGRVYSSRNSGMFMDI
HDASGKIQIFSHKDTTPEEARALLPMIDIGDIIGVTGIVRRTKRGELTINAQKIEMLTKSLLPMPEKWHGLSDIELRYRK
RHLDIMTNEDSKLRFQQRSKIVSGIRRFMENDGFMEVETPMLQSIYGGATAEPFKTHHNTLKLDMYLRIAPELFLKRTLV
SGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDMMDLVERLFETLALSIHGTTEFDFGDKQLSFKGPFKRVP
MPDAVKQVTGIDFLAIKTDEEARTAAKAAGFAVEKDWTWGECLAFIFEEKVESTLIQPSHVTHFPKDISPFAKEVPGEPR
LVERFETYCNAWELGNAFSELNDPEEQRRRMVEQLEQAHARGEKEKQLDEEFLDAIDQGMPPAGGLGIGVDRLIMLLTNA
PSIRDVILFPARRNKAD

Sequences:

>Translated_497_residues
MNDKTENTLSSDVTEVRAQKLKLLREQVGDVYPAHFHRTLTNAELGAKYESLESDVETQDVVTVAGRVYSSRNSGMFMDI
HDASGKIQIFSHKDTTPEEARALLPMIDIGDIIGVTGIVRRTKRGELTINAQKIEMLTKSLLPMPEKWHGLSDIELRYRK
RHLDIMTNEDSKLRFQQRSKIVSGIRRFMENDGFMEVETPMLQSIYGGATAEPFKTHHNTLKLDMYLRIAPELFLKRTLV
SGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDMMDLVERLFETLALSIHGTTEFDFGDKQLSFKGPFKRVP
MPDAVKQVTGIDFLAIKTDEEARTAAKAAGFAVEKDWTWGECLAFIFEEKVESTLIQPSHVTHFPKDISPFAKEVPGEPR
LVERFETYCNAWELGNAFSELNDPEEQRRRMVEQLEQAHARGEKEKQLDEEFLDAIDQGMPPAGGLGIGVDRLIMLLTNA
PSIRDVILFPARRNKAD
>Mature_497_residues
MNDKTENTLSSDVTEVRAQKLKLLREQVGDVYPAHFHRTLTNAELGAKYESLESDVETQDVVTVAGRVYSSRNSGMFMDI
HDASGKIQIFSHKDTTPEEARALLPMIDIGDIIGVTGIVRRTKRGELTINAQKIEMLTKSLLPMPEKWHGLSDIELRYRK
RHLDIMTNEDSKLRFQQRSKIVSGIRRFMENDGFMEVETPMLQSIYGGATAEPFKTHHNTLKLDMYLRIAPELFLKRTLV
SGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDMMDLVERLFETLALSIHGTTEFDFGDKQLSFKGPFKRVP
MPDAVKQVTGIDFLAIKTDEEARTAAKAAGFAVEKDWTWGECLAFIFEEKVESTLIQPSHVTHFPKDISPFAKEVPGEPR
LVERFETYCNAWELGNAFSELNDPEEQRRRMVEQLEQAHARGEKEKQLDEEFLDAIDQGMPPAGGLGIGVDRLIMLLTNA
PSIRDVILFPARRNKAD

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=499, Percent_Identity=36.0721442885772, Blast_Score=310, Evalue=3e-84,
Organism=Homo sapiens, GI194272210, Length=499, Percent_Identity=36.0721442885772, Blast_Score=308, Evalue=6e-84,
Organism=Homo sapiens, GI40789249, Length=248, Percent_Identity=25.4032258064516, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI226491502, Length=497, Percent_Identity=22.3340040241449, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1789256, Length=485, Percent_Identity=47.4226804123711, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1790571, Length=484, Percent_Identity=46.6942148760331, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=31.6923076923077, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788173, Length=318, Percent_Identity=28.6163522012579, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787161, Length=472, Percent_Identity=24.5762711864407, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535925, Length=505, Percent_Identity=39.6039603960396, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17535927, Length=505, Percent_Identity=39.6039603960396, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI71994340, Length=497, Percent_Identity=39.4366197183099, Blast_Score=344, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI32566633, Length=281, Percent_Identity=26.6903914590747, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=507, Percent_Identity=38.8560157790927, Blast_Score=336, Evalue=5e-93,
Organism=Saccharomyces cerevisiae, GI6324256, Length=476, Percent_Identity=28.781512605042, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6321807, Length=454, Percent_Identity=25.9911894273128, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24640849, Length=493, Percent_Identity=39.7565922920893, Blast_Score=338, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24640851, Length=493, Percent_Identity=39.7565922920893, Blast_Score=338, Evalue=6e-93,
Organism=Drosophila melanogaster, GI24584738, Length=248, Percent_Identity=28.2258064516129, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56748; Mature: 56748

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKTENTLSSDVTEVRAQKLKLLREQVGDVYPAHFHRTLTNAELGAKYESLESDVETQD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCHHHHHHCCCCHHH
VVTVAGRVYSSRNSGMFMDIHDASGKIQIFSHKDTTPEEARALLPMIDIGDIIGVTGIVR
HHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHH
RTKRGELTINAQKIEMLTKSLLPMPEKWHGLSDIELRYRKRHLDIMTNEDSKLRFQQRSK
HCCCCCEEEEHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHH
IVSGIRRFMENDGFMEVETPMLQSIYGGATAEPFKTHHNTLKLDMYLRIAPELFLKRTLV
HHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCCHHHCCCEEEEEEEEHHHHHHHHHHHHH
SGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDMMDLVERLFETLALSIHGT
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHEEEECCC
TEFDFGDKQLSFKGPFKRVPMPDAVKQVTGIDFLAIKTDEEARTAAKAAGFAVEKDWTWG
EECCCCCCEECCCCCCCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHCCEEECCCCHH
ECLAFIFEEKVESTLIQPSHVTHFPKDISPFAKEVPGEPRLVERFETYCNAWELGNAFSE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHH
LNDPEEQRRRMVEQLEQAHARGEKEKQLDEEFLDAIDQGMPPAGGLGIGVDRLIMLLTNA
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCC
PSIRDVILFPARRNKAD
CCCCEEEEECCCCCCCC
>Mature Secondary Structure
MNDKTENTLSSDVTEVRAQKLKLLREQVGDVYPAHFHRTLTNAELGAKYESLESDVETQD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCHHHHHHCCCCHHH
VVTVAGRVYSSRNSGMFMDIHDASGKIQIFSHKDTTPEEARALLPMIDIGDIIGVTGIVR
HHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHH
RTKRGELTINAQKIEMLTKSLLPMPEKWHGLSDIELRYRKRHLDIMTNEDSKLRFQQRSK
HCCCCCEEEEHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHH
IVSGIRRFMENDGFMEVETPMLQSIYGGATAEPFKTHHNTLKLDMYLRIAPELFLKRTLV
HHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCCHHHCCCEEEEEEEEHHHHHHHHHHHHH
SGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDMMDLVERLFETLALSIHGT
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHEEEECCC
TEFDFGDKQLSFKGPFKRVPMPDAVKQVTGIDFLAIKTDEEARTAAKAAGFAVEKDWTWG
EECCCCCCEECCCCCCCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHCCEEECCCCHH
ECLAFIFEEKVESTLIQPSHVTHFPKDISPFAKEVPGEPRLVERFETYCNAWELGNAFSE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHH
LNDPEEQRRRMVEQLEQAHARGEKEKQLDEEFLDAIDQGMPPAGGLGIGVDRLIMLLTNA
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCC
PSIRDVILFPARRNKAD
CCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA