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The map label for this gene is gltX [H]

Identifier: 209550995

GI number: 209550995

Start: 3534985

End: 3536439

Strand: Direct

Name: gltX [H]

Synonym: Rleg2_3419

Alternate gene names: 209550995

Gene position: 3534985-3536439 (Clockwise)

Preceding gene: 209550994

Following gene: 209550996

Centisome position: 77.9

GC content: 61.79

Gene sequence:

>1455_bases
ATGACAACTTCCGGCGTCCGCGTCCGCATCGCTCCCTCGCCGACCGGCGAGCCGCATGTCGGCACCGCTTACATCGCTCT
CTTCAACTACCTTTTCGCCAAGAAGCACGGCGGCGAGTTCATCTTGCGCATCGAGGATACCGACGCGACGCGCTCGACCC
CGGAATTCGAGACCAAGGTATTGGACGCCTTGAAATGGTGCGGGCTGGAATGGAAGGAAGGCCCCGATATCGGCGGTCCC
TACGGCCCCTATCGCCAGTCCGACCGCAAGCCGATGTACCAGCCTTACGCTGAGGAGCTGCTGGACAAGGGCCATGCCTT
CCGCTGTTTCTGCACGCCCGCGCGTCTCGAACAGATGCGCGAGGCGCAGCGCGCCGCCGGCAAGCCGCCGAAATATGACG
GGCTCTGCCTCAGCCTGACGGCAGAGGAAGTGACCTCGCGCATGGCAGCCGGCGAGACGACCGTCATCCGCATGAAGATC
CCGGCTGAAGGCTCCTGCGACTTCACCGACGGCGTCTACGGCGATGTCTCCATCCCCTGGGATTCCGTCGACATGCAGGT
GCTGATCAAGGCCGACGGCATGCCGACCTATCACATGGCCAACGTCATCGACGACCATCTGATGAAGATCACCCATGTGG
CGCGCGGCGAGGAATGGCTGGCTTCGGTGCCGAAGCACATCCTGCTCTATCGCTATTTCGGCTGGGACCAGCCGGTCTTC
ATGCATCTGTCGCTGATGCGCAATGCCGACAAGTCGAAGCTGTCGAAGCGCAAGAACCCGACCTCGATCTCCTATTATTC
TGCGCTCGGTTATATCCCCGAAGCGCTGATGAATTTTCTCGGCCTGTTCTTCATCCAGATCGCCGAAGGCGAAGAGCTGT
TGACGATGGAGGAGCTCTCCGCGAAGTTCGATCCGGAGAACCTCTCCAAGGCCGGCGCGATCTTCGACATCCAGAAACTC
GACTGGCTGAACGGCCGCTGGATCCGCGAGAAGCTCTCCGAGGAAGAATTCCAGGCGCGTGTGCTCGCCTGGGCGATGGA
AAACGATCGTTTGAAGGCGGGTCTCCGCCTGTCGCAGACGCGCATTTCCAAGCTCGGCGAACTGCCCGATCTGGCCGGCT
TCCTGCTGAAGTCCGATCTCGGCCTGCAGCCGTCCGACTTCGCCAAGATCAAGTCTCCGCCGGAAGAGATCCTGGAGATC
CTCAACACCGTTCAGCCGGATCTGGAAAAGATCCTGGAATGGAATGTCGAGACGATCGAGGCAGAGCTGCGCGCCATCGC
CGACCGCATGGGCAAGAAGCTGAAGGTCGTGGTCTCGCCGCTCTTCGTCGCCGTATCAGGCTCGTCGCGTTCGCTGCCGC
TCTTCGATTCCATGGCGATCCTCGGCCGCTCCGTCGTGCGCCAGCGCCTGAAGCTCGCAGCCCAGGCCGTCGCCGCCCTC
GTCGGCTCGAAATAA

Upstream 100 bases:

>100_bases
GCCCTTTTTCGGCCGGTTTGCCGATGAAGATATCTGCATCCTTCGGCCGAACGTCGCTTTCCCCGGAAAGCCGTCCGGCG
TTCATTGACGGGCCATACCA

Downstream 100 bases:

>100_bases
TAGTGGGAGCCCCTCCCCCTTGTGGGGAGGGGTTGGGGAGGGGCCTTTTCCAGAAAACCCCTCCCCTCCGCTTCGCTCCG
ACCCTCCCCACAAGGAGAGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTTSGVRVRIAPSPTGEPHVGTAYIALFNYLFAKKHGGEFILRIEDTDATRSTPEFETKVLDALKWCGLEWKEGPDIGGP
YGPYRQSDRKPMYQPYAEELLDKGHAFRCFCTPARLEQMREAQRAAGKPPKYDGLCLSLTAEEVTSRMAAGETTVIRMKI
PAEGSCDFTDGVYGDVSIPWDSVDMQVLIKADGMPTYHMANVIDDHLMKITHVARGEEWLASVPKHILLYRYFGWDQPVF
MHLSLMRNADKSKLSKRKNPTSISYYSALGYIPEALMNFLGLFFIQIAEGEELLTMEELSAKFDPENLSKAGAIFDIQKL
DWLNGRWIREKLSEEEFQARVLAWAMENDRLKAGLRLSQTRISKLGELPDLAGFLLKSDLGLQPSDFAKIKSPPEEILEI
LNTVQPDLEKILEWNVETIEAELRAIADRMGKKLKVVVSPLFVAVSGSSRSLPLFDSMAILGRSVVRQRLKLAAQAVAAL
VGSK

Sequences:

>Translated_484_residues
MTTSGVRVRIAPSPTGEPHVGTAYIALFNYLFAKKHGGEFILRIEDTDATRSTPEFETKVLDALKWCGLEWKEGPDIGGP
YGPYRQSDRKPMYQPYAEELLDKGHAFRCFCTPARLEQMREAQRAAGKPPKYDGLCLSLTAEEVTSRMAAGETTVIRMKI
PAEGSCDFTDGVYGDVSIPWDSVDMQVLIKADGMPTYHMANVIDDHLMKITHVARGEEWLASVPKHILLYRYFGWDQPVF
MHLSLMRNADKSKLSKRKNPTSISYYSALGYIPEALMNFLGLFFIQIAEGEELLTMEELSAKFDPENLSKAGAIFDIQKL
DWLNGRWIREKLSEEEFQARVLAWAMENDRLKAGLRLSQTRISKLGELPDLAGFLLKSDLGLQPSDFAKIKSPPEEILEI
LNTVQPDLEKILEWNVETIEAELRAIADRMGKKLKVVVSPLFVAVSGSSRSLPLFDSMAILGRSVVRQRLKLAAQAVAAL
VGSK
>Mature_483_residues
TTSGVRVRIAPSPTGEPHVGTAYIALFNYLFAKKHGGEFILRIEDTDATRSTPEFETKVLDALKWCGLEWKEGPDIGGPY
GPYRQSDRKPMYQPYAEELLDKGHAFRCFCTPARLEQMREAQRAAGKPPKYDGLCLSLTAEEVTSRMAAGETTVIRMKIP
AEGSCDFTDGVYGDVSIPWDSVDMQVLIKADGMPTYHMANVIDDHLMKITHVARGEEWLASVPKHILLYRYFGWDQPVFM
HLSLMRNADKSKLSKRKNPTSISYYSALGYIPEALMNFLGLFFIQIAEGEELLTMEELSAKFDPENLSKAGAIFDIQKLD
WLNGRWIREKLSEEEFQARVLAWAMENDRLKAGLRLSQTRISKLGELPDLAGFLLKSDLGLQPSDFAKIKSPPEEILEIL
NTVQPDLEKILEWNVETIEAELRAIADRMGKKLKVVVSPLFVAVSGSSRSLPLFDSMAILGRSVVRQRLKLAAQAVAALV
GSK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=499, Percent_Identity=34.4689378757515, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1788743, Length=481, Percent_Identity=33.8877338877339, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI226510925, Length=264, Percent_Identity=30.3030303030303, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71993394, Length=484, Percent_Identity=35.3305785123967, Blast_Score=278, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6324540, Length=341, Percent_Identity=41.3489736070381, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=38.0855397148676, Blast_Score=298, Evalue=8e-81,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 54238; Mature: 54107

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSGVRVRIAPSPTGEPHVGTAYIALFNYLFAKKHGGEFILRIEDTDATRSTPEFETKV
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHH
LDALKWCGLEWKEGPDIGGPYGPYRQSDRKPMYQPYAEELLDKGHAFRCFCTPARLEQMR
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECHHHHHHHH
EAQRAAGKPPKYDGLCLSLTAEEVTSRMAAGETTVIRMKIPAEGSCDFTDGVYGDVSIPW
HHHHHCCCCCCCCCEEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
DSVDMQVLIKADGMPTYHMANVIDDHLMKITHVARGEEWLASVPKHILLYRYFGWDQPVF
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEHHHCCCCCHHH
MHLSLMRNADKSKLSKRKNPTSISYYSALGYIPEALMNFLGLFFIQIAEGEELLTMEELS
HHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHH
AKFDPENLSKAGAIFDIQKLDWLNGRWIREKLSEEEFQARVLAWAMENDRLKAGLRLSQT
CCCCHHHHHHCCCEEEHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCHHHHH
RISKLGELPDLAGFLLKSDLGLQPSDFAKIKSPPEEILEILNTVQPDLEKILEWNVETIE
HHHHHCCCHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHH
AELRAIADRMGKKLKVVVSPLFVAVSGSSRSLPLFDSMAILGRSVVRQRLKLAAQAVAAL
HHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGSK
HCCC
>Mature Secondary Structure 
TTSGVRVRIAPSPTGEPHVGTAYIALFNYLFAKKHGGEFILRIEDTDATRSTPEFETKV
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHH
LDALKWCGLEWKEGPDIGGPYGPYRQSDRKPMYQPYAEELLDKGHAFRCFCTPARLEQMR
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECHHHHHHHH
EAQRAAGKPPKYDGLCLSLTAEEVTSRMAAGETTVIRMKIPAEGSCDFTDGVYGDVSIPW
HHHHHCCCCCCCCCEEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
DSVDMQVLIKADGMPTYHMANVIDDHLMKITHVARGEEWLASVPKHILLYRYFGWDQPVF
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEHHHCCCCCHHH
MHLSLMRNADKSKLSKRKNPTSISYYSALGYIPEALMNFLGLFFIQIAEGEELLTMEELS
HHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHH
AKFDPENLSKAGAIFDIQKLDWLNGRWIREKLSEEEFQARVLAWAMENDRLKAGLRLSQT
CCCCHHHHHHCCCEEEHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCHHHHH
RISKLGELPDLAGFLLKSDLGLQPSDFAKIKSPPEEILEILNTVQPDLEKILEWNVETIE
HHHHHCCCHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHH
AELRAIADRMGKKLKVVVSPLFVAVSGSSRSLPLFDSMAILGRSVVRQRLKLAAQAVAAL
HHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGSK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA