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The map label for this gene is rbsB [H]

Identifier: 209550983

GI number: 209550983

Start: 3520002

End: 3520949

Strand: Reverse

Name: rbsB [H]

Synonym: Rleg2_3407

Alternate gene names: 209550983

Gene position: 3520949-3520002 (Counterclockwise)

Preceding gene: 209550984

Following gene: 209550982

Centisome position: 77.59

GC content: 61.81

Gene sequence:

>948_bases
ATGCGTAAATTGATGTTGGGCCTGGCGGTTGCGGCGGTGACGTTCGCCGGCGCCGCTCACGCCGAGGACAAGAAATTCAC
CATCGGCGTGTCCATTCCGGCCGCCGACCACGGCTGGACGTCGGGCGTGGTCTTCCATGCCGAACGTGTCGCCAAGTTGC
TGATGGCCGAACATCCCGGTCTCAACGTCATCGTCAAGACCTCGCCGGACGCTGCGAGCCAGGCAAACGCCGTGCAGGAT
CTCGACACGCAGGGCATCGACGCCCTGGTCATCCTGCCGTCGGATCCGGATCCGCTCGTCAACGCTATCAAGGAAGTCAA
GGGCAAGGGCAAGTTCGTCGCGCTCGTCGACCGTGCGCCGTCGAGCAACGACAACTCCGTGCGCGACCTCTATGTCGCCG
GCAACAATCCGGCGCTTGGCGAAGTCGCCGGCAAGTACATCAAGGACACCACGCCGGATGCCGAAGTCGTGATTATCCGC
GGTCTGCCGATCCCGATCGACCAGCAGCGCCAGGACGGCTTCGACAAGGGCATTGCCGGCTCGAAGGTCAAGGTTCTCGA
CCGCCAGTACGGCAACTGGAACCGCGACGATGCCTTCAAGGTCATGCAGGACTACCTGACCAAGTACCAGAAGATCGACG
TCGTTTGGTGCCAGGACGATGACATGGCCGTCGGCGTTCTGCAGGCGATCGAGCAGGCCAAGCGCACCGACATCAAATAT
GTCGTCGCCGGGGCCGGCTCCAAGGACATGGTCAAGAAGGTCATGGACGGCGACAAGATGATCCCGGTCGACGTTCTCTA
TCCGCCGGCAATGGTCGGCACGGCCATGGAACTGACGGCCGCTGCCCTCTACGATCAAGTCCCGGTTCACGGCAGCTATA
TCCTCGACGCGACCCTCGTCACCAAGGACAATGCCAAGGACTTCTACTTCCCGGATTCTCCGTTCTGA

Upstream 100 bases:

>100_bases
ATTGCTCCGGTTTGCATTGATCGGGCTCAGGATTGACCGGACTTACAGGGCGTCCGGTCCCGATATGGAAAAGTCCCTGC
AATTATTGGGAGGAAATGGC

Downstream 100 bases:

>100_bases
TCCATTGGCTGGCGACATGCGCCCATTCGAAAGGATGGGCGCATTTTATGCTTCCATATACGTACGCAGCTGAAAATGCT
GCACGTACCTCTTGCCCGCC

Product: sugar ABC transporter substrate-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRKLMLGLAVAAVTFAGAAHAEDKKFTIGVSIPAADHGWTSGVVFHAERVAKLLMAEHPGLNVIVKTSPDAASQANAVQD
LDTQGIDALVILPSDPDPLVNAIKEVKGKGKFVALVDRAPSSNDNSVRDLYVAGNNPALGEVAGKYIKDTTPDAEVVIIR
GLPIPIDQQRQDGFDKGIAGSKVKVLDRQYGNWNRDDAFKVMQDYLTKYQKIDVVWCQDDDMAVGVLQAIEQAKRTDIKY
VVAGAGSKDMVKKVMDGDKMIPVDVLYPPAMVGTAMELTAAALYDQVPVHGSYILDATLVTKDNAKDFYFPDSPF

Sequences:

>Translated_315_residues
MRKLMLGLAVAAVTFAGAAHAEDKKFTIGVSIPAADHGWTSGVVFHAERVAKLLMAEHPGLNVIVKTSPDAASQANAVQD
LDTQGIDALVILPSDPDPLVNAIKEVKGKGKFVALVDRAPSSNDNSVRDLYVAGNNPALGEVAGKYIKDTTPDAEVVIIR
GLPIPIDQQRQDGFDKGIAGSKVKVLDRQYGNWNRDDAFKVMQDYLTKYQKIDVVWCQDDDMAVGVLQAIEQAKRTDIKY
VVAGAGSKDMVKKVMDGDKMIPVDVLYPPAMVGTAMELTAAALYDQVPVHGSYILDATLVTKDNAKDFYFPDSPF
>Mature_315_residues
MRKLMLGLAVAAVTFAGAAHAEDKKFTIGVSIPAADHGWTSGVVFHAERVAKLLMAEHPGLNVIVKTSPDAASQANAVQD
LDTQGIDALVILPSDPDPLVNAIKEVKGKGKFVALVDRAPSSNDNSVRDLYVAGNNPALGEVAGKYIKDTTPDAEVVIIR
GLPIPIDQQRQDGFDKGIAGSKVKVLDRQYGNWNRDDAFKVMQDYLTKYQKIDVVWCQDDDMAVGVLQAIEQAKRTDIKY
VVAGAGSKDMVKKVMDGDKMIPVDVLYPPAMVGTAMELTAAALYDQVPVHGSYILDATLVTKDNAKDFYFPDSPF

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=250, Percent_Identity=30, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790674, Length=312, Percent_Identity=25.9615384615385, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789990, Length=283, Percent_Identity=26.8551236749117, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34002; Mature: 34002

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLMLGLAVAAVTFAGAAHAEDKKFTIGVSIPAADHGWTSGVVFHAERVAKLLMAEHPG
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHCCCC
LNVIVKTSPDAASQANAVQDLDTQGIDALVILPSDPDPLVNAIKEVKGKGKFVALVDRAP
CEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCC
SSNDNSVRDLYVAGNNPALGEVAGKYIKDTTPDAEVVIIRGLPIPIDQQRQDGFDKGIAG
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCC
SKVKVLDRQYGNWNRDDAFKVMQDYLTKYQKIDVVWCQDDDMAVGVLQAIEQAKRTDIKY
CEEEEEEHHCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCEEE
VVAGAGSKDMVKKVMDGDKMIPVDVLYPPAMVGTAMELTAAALYDQVPVHGSYILDATLV
EEECCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
TKDNAKDFYFPDSPF
ECCCCCCCCCCCCCC
>Mature Secondary Structure
MRKLMLGLAVAAVTFAGAAHAEDKKFTIGVSIPAADHGWTSGVVFHAERVAKLLMAEHPG
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHCCCC
LNVIVKTSPDAASQANAVQDLDTQGIDALVILPSDPDPLVNAIKEVKGKGKFVALVDRAP
CEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCC
SSNDNSVRDLYVAGNNPALGEVAGKYIKDTTPDAEVVIIRGLPIPIDQQRQDGFDKGIAG
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCC
SKVKVLDRQYGNWNRDDAFKVMQDYLTKYQKIDVVWCQDDDMAVGVLQAIEQAKRTDIKY
CEEEEEEHHCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCEEE
VVAGAGSKDMVKKVMDGDKMIPVDVLYPPAMVGTAMELTAAALYDQVPVHGSYILDATLV
EEECCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
TKDNAKDFYFPDSPF
ECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]