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The map label for this gene is ycdT [H]

Identifier: 209550963

GI number: 209550963

Start: 3499672

End: 3500829

Strand: Reverse

Name: ycdT [H]

Synonym: Rleg2_3387

Alternate gene names: 209550963

Gene position: 3500829-3499672 (Counterclockwise)

Preceding gene: 209550964

Following gene: 209550958

Centisome position: 77.15

GC content: 61.31

Gene sequence:

>1158_bases
ATGCTGCTGGATCTCAGGACGATCTATTTCATTGTTGCCGTGAGCTGCTTTGTGCTTGGTATTCTCCAGCTCGCAGCCTA
TGCGACCGGCCGCTTCGAGAGATGGCCGCTCTGGTGGGGCCTGAGCAACCTGCTTGTCGGCGTCGGTTCGTTCCTCGTCG
CGCTGCGCAATCTCGTTCCCTATTCGGTCTCGGTCGACGGCGGCAATATCGTGACCATTGCCGGCTACATGCTGATGTTC
TTCGCCGTGCGGGTCTTTGCCGGCCGGGCCCTTAACCAGCGCTATTTCTGGCTTGCCATCTTTCTCGTCAGCGTGCCTGT
CGCACTTATTGTCAGCGATCCCTCGGCGGTGTCGGCGAGGCTCCTCTATGTCTCGGTCATCTGCTGTCTCTGCGACCTTG
CCGTCGCAAGTGAGGCAGTCGGTGTAGCCCGGCGTGAGAAGCTTTATTCGGCAGTCCTGCTCGTCGGTCTGTATGTCTGC
ACTGCGATGATCTTTGCGGTGCGCAGCATTCTTGCCGCGACCGGCGAGATCGGCGGGCCGGATCCTTTCGGCGGCAGTGT
CGTCCATAGCTGGATGGCGGTATCGGCGGTCGCGTTCATCATGCTGCGCGGCATGGCTATGGTACTGATGGCCGCCGAGC
GCAGCCGAAATCAGCTGACCGAACTCGCCCACCACGATCCGCTGACCGGCGCGCTCAATCGCGGCGGCCTTGCCCAGCGC
CTGCCGGCTCTCGGTTCCCAGCCGGTATCGCTGCTCATCATCGACATCGACCATTTCAAGCAATTGAACGACCGGCATGG
CCATGCGAGTGGCGACGATATCCTGCGGCTTTTCGCCAGTGTTTCGAGAAGCATCATGGGCTCGAACGATCTGCTCGCCC
GTCAGGGTGGCGACGAGTTCCTGGTGGTGCTGAAGGATGTTTCCGGGCAGGATGCGGTGGCGATTGCCGAGCGCATCCGT
CTCGCCTTCGCCGCCGCCGTCCTGCATCAGCCGGATCTGGCCGTCTTTCCGACGCTGAGCATCGGCGTTGCCACGCGCGG
GAAAAGCGGCGGAGATTTTGAACAGCTGATAAAGAAGGCGGATGAGGCGCTTTACCGTTCGAAGCGCGAAGGCCGCAACC
GGGTCAAAGCCTTCGGCGAAAATCAGCAGGCTGCGTAA

Upstream 100 bases:

>100_bases
AGCCGCATGAAAAACCGCTGATCGTTAAACTTCTAGCGAAGCCCCCAATCAAACCGCAAAACGCTTCCTGTACGTTCCTT
GAGAATTTCGGGAACGCGTC

Downstream 100 bases:

>100_bases
CGGACCGGGTTTGTTTTAGGCAATTCCGGATGCAAAGCCACTTCACGCTTTTGCTGGAATTGCTCTAATTGTGCGTCGAC
GCGCTTTAGCTCTTGTTTTT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MLLDLRTIYFIVAVSCFVLGILQLAAYATGRFERWPLWWGLSNLLVGVGSFLVALRNLVPYSVSVDGGNIVTIAGYMLMF
FAVRVFAGRALNQRYFWLAIFLVSVPVALIVSDPSAVSARLLYVSVICCLCDLAVASEAVGVARREKLYSAVLLVGLYVC
TAMIFAVRSILAATGEIGGPDPFGGSVVHSWMAVSAVAFIMLRGMAMVLMAAERSRNQLTELAHHDPLTGALNRGGLAQR
LPALGSQPVSLLIIDIDHFKQLNDRHGHASGDDILRLFASVSRSIMGSNDLLARQGGDEFLVVLKDVSGQDAVAIAERIR
LAFAAAVLHQPDLAVFPTLSIGVATRGKSGGDFEQLIKKADEALYRSKREGRNRVKAFGENQQAA

Sequences:

>Translated_385_residues
MLLDLRTIYFIVAVSCFVLGILQLAAYATGRFERWPLWWGLSNLLVGVGSFLVALRNLVPYSVSVDGGNIVTIAGYMLMF
FAVRVFAGRALNQRYFWLAIFLVSVPVALIVSDPSAVSARLLYVSVICCLCDLAVASEAVGVARREKLYSAVLLVGLYVC
TAMIFAVRSILAATGEIGGPDPFGGSVVHSWMAVSAVAFIMLRGMAMVLMAAERSRNQLTELAHHDPLTGALNRGGLAQR
LPALGSQPVSLLIIDIDHFKQLNDRHGHASGDDILRLFASVSRSIMGSNDLLARQGGDEFLVVLKDVSGQDAVAIAERIR
LAFAAAVLHQPDLAVFPTLSIGVATRGKSGGDFEQLIKKADEALYRSKREGRNRVKAFGENQQAA
>Mature_385_residues
MLLDLRTIYFIVAVSCFVLGILQLAAYATGRFERWPLWWGLSNLLVGVGSFLVALRNLVPYSVSVDGGNIVTIAGYMLMF
FAVRVFAGRALNQRYFWLAIFLVSVPVALIVSDPSAVSARLLYVSVICCLCDLAVASEAVGVARREKLYSAVLLVGLYVC
TAMIFAVRSILAATGEIGGPDPFGGSVVHSWMAVSAVAFIMLRGMAMVLMAAERSRNQLTELAHHDPLTGALNRGGLAQR
LPALGSQPVSLLIIDIDHFKQLNDRHGHASGDDILRLFASVSRSIMGSNDLLARQGGDEFLVVLKDVSGQDAVAIAERIR
LAFAAAVLHQPDLAVFPTLSIGVATRGKSGGDFEQLIKKADEALYRSKREGRNRVKAFGENQQAA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=183, Percent_Identity=34.4262295081967, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787802, Length=174, Percent_Identity=36.7816091954023, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081881, Length=177, Percent_Identity=38.9830508474576, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI145693134, Length=174, Percent_Identity=40.8045977011494, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=39.2638036809816, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788381, Length=191, Percent_Identity=35.6020942408377, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787541, Length=204, Percent_Identity=30.3921568627451, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=32.9479768786127, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=29.6296296296296, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788956, Length=163, Percent_Identity=34.3558282208589, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081977, Length=157, Percent_Identity=29.9363057324841, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787816, Length=169, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=151, Percent_Identity=31.7880794701987, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=160, Percent_Identity=32.5, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41538; Mature: 41538

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDLRTIYFIVAVSCFVLGILQLAAYATGRFERWPLWWGLSNLLVGVGSFLVALRNLVP
CEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
YSVSVDGGNIVTIAGYMLMFFAVRVFAGRALNQRYFWLAIFLVSVPVALIVSDPSAVSAR
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHEEECCCHHHHHH
LLYVSVICCLCDLAVASEAVGVARREKLYSAVLLVGLYVCTAMIFAVRSILAATGEIGGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DPFGGSVVHSWMAVSAVAFIMLRGMAMVLMAAERSRNQLTELAHHDPLTGALNRGGLAQR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LPALGSQPVSLLIIDIDHFKQLNDRHGHASGDDILRLFASVSRSIMGSNDLLARQGGDEF
HCCCCCCCCEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCE
LVVLKDVSGQDAVAIAERIRLAFAAAVLHQPDLAVFPTLSIGVATRGKSGGDFEQLIKKA
EEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCEECEEECCCCCCCHHHHHHHH
DEALYRSKREGRNRVKAFGENQQAA
HHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLLDLRTIYFIVAVSCFVLGILQLAAYATGRFERWPLWWGLSNLLVGVGSFLVALRNLVP
CEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
YSVSVDGGNIVTIAGYMLMFFAVRVFAGRALNQRYFWLAIFLVSVPVALIVSDPSAVSAR
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHEEECCCHHHHHH
LLYVSVICCLCDLAVASEAVGVARREKLYSAVLLVGLYVCTAMIFAVRSILAATGEIGGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DPFGGSVVHSWMAVSAVAFIMLRGMAMVLMAAERSRNQLTELAHHDPLTGALNRGGLAQR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LPALGSQPVSLLIIDIDHFKQLNDRHGHASGDDILRLFASVSRSIMGSNDLLARQGGDEF
HCCCCCCCCEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCE
LVVLKDVSGQDAVAIAERIRLAFAAAVLHQPDLAVFPTLSIGVATRGKSGGDFEQLIKKA
EEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCEECEEECCCCCCCHHHHHHHH
DEALYRSKREGRNRVKAFGENQQAA
HHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]