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The map label for this gene is pntAA [H]

Identifier: 209550944

GI number: 209550944

Start: 3479052

End: 3480200

Strand: Reverse

Name: pntAA [H]

Synonym: Rleg2_3368

Alternate gene names: 209550944

Gene position: 3480200-3479052 (Counterclockwise)

Preceding gene: 209550945

Following gene: 209550943

Centisome position: 76.69

GC content: 65.27

Gene sequence:

>1149_bases
TTGGGCAATATCGTTTTCGTTGCAAGGGAAATTGCGGGCGAGGAGACGCGTGTCGCGGCCTCCGCCGAGACCGTGAAAAA
AATGAAAAGTTTCGGCTTTGACGTCGTCGTCGAAGCCGGTGCCGGTGCGGCGTCGCGCCTTCCGGACAGAGAGTTCGAGG
CCGCCGGCGCCAGGATCGGTACTTCTGCCGATGCAGCCTCCGCCGACGTGGTGCTGAAGGTGCGCCGCCCGAGCGAACAG
GAAATCGCAGGCTATAAAAGCGGCGCCGTGGTCATCGCCATCATGGATCCCTACGGCAATGACGAAGCGATCGCGGCTCT
TGCCAGCGCCGGTCTTTCGGCTTTCGCGATGGAACTGATGCCGCGCATTACCCGCGCCCAGTCCATGGACGTGCTTTCGT
CCCAGGCCAATCTTGCCGGCTATCAGGCTGTCATCGAGGCGGCGGCGGTTTATGACCGCGCCATGCCGATGATGATGACG
GCTGCCGGCACCGTGCCGGCCGCCAAGGTCTTCGTCATGGGCGCCGGCGTCGCCGGTCTCCAGGCGATCGCCACGGCGCG
GCGCCTGGGTGCTGCGGTTTCGGCCACCGATGTGCGCCCGGCCGCCAAGGAGCAGGTCGCCTCGCTCGGCGCCAAGTTCA
TCGCCGTCGAGGACGAGGAGTTCAAGGCGGCGGAAACGGCCGGCGGTTACGCCAAGGAAATGTCCGCTGACTATCAGGCA
AAGCAAGCCGCTCTGGTGGCCGAGCATATCGCCAAGCAGGATATCGTCATCACCACGGCGCTGATCCCCGGCCGCGCCGC
ACCGCGGCTCGTTTCGCGCGCCATGCTCGCCGCGATGAAGCCGGGTGCGGTCGCGGTCGATCTCGCGGTCGAGCGCGGCG
GCAATATCGAGGGCGTCGTTGCCGGCGAGATCACCGATGTCGATGGCGTCAAGGTGATCGGTTTTGCCAACATGGCGGGC
CGGGTCGCTGCCAGCGCCTCGGCGCTCTACGCCAAGAACCTCGTCACCTTCCTCGAGACCATGGTCAACAAGGAGAGCCG
GAGCGTCGTCGTCAACCTCGACGACGAACTCGTCAAGGCGACGATGCTGACCTATGCCGGCGACGTGGTGCATCCCGCCT
TCGGCGGCGCGAAGAAGGGAGATCTCTGA

Upstream 100 bases:

>100_bases
TTATCGCAGGACTGCCGGACAGTTTTGCGCGACATGCTTTAGGTGCTTGGCCTGCGCAGGGAAGCCTGCGGCGGTCTGGC
TGACCCGGAGGAGGGGGCAG

Downstream 100 bases:

>100_bases
TGGCCAGTGAAGCAATGGACAAAGCGCTGGAGCAGCTCGACCAGGCGGTCACCGCCGTCGTCACGGCAGCGGCCCATGCG
CCGGAAGCGGCCAGTGCTGC

Product: alanine dehydrogenase/PNT domain-containing protein

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MGNIVFVAREIAGEETRVAASAETVKKMKSFGFDVVVEAGAGAASRLPDREFEAAGARIGTSADAASADVVLKVRRPSEQ
EIAGYKSGAVVIAIMDPYGNDEAIAALASAGLSAFAMELMPRITRAQSMDVLSSQANLAGYQAVIEAAAVYDRAMPMMMT
AAGTVPAAKVFVMGAGVAGLQAIATARRLGAAVSATDVRPAAKEQVASLGAKFIAVEDEEFKAAETAGGYAKEMSADYQA
KQAALVAEHIAKQDIVITTALIPGRAAPRLVSRAMLAAMKPGAVAVDLAVERGGNIEGVVAGEITDVDGVKVIGFANMAG
RVAASASALYAKNLVTFLETMVNKESRSVVVNLDDELVKATMLTYAGDVVHPAFGGAKKGDL

Sequences:

>Translated_382_residues
MGNIVFVAREIAGEETRVAASAETVKKMKSFGFDVVVEAGAGAASRLPDREFEAAGARIGTSADAASADVVLKVRRPSEQ
EIAGYKSGAVVIAIMDPYGNDEAIAALASAGLSAFAMELMPRITRAQSMDVLSSQANLAGYQAVIEAAAVYDRAMPMMMT
AAGTVPAAKVFVMGAGVAGLQAIATARRLGAAVSATDVRPAAKEQVASLGAKFIAVEDEEFKAAETAGGYAKEMSADYQA
KQAALVAEHIAKQDIVITTALIPGRAAPRLVSRAMLAAMKPGAVAVDLAVERGGNIEGVVAGEITDVDGVKVIGFANMAG
RVAASASALYAKNLVTFLETMVNKESRSVVVNLDDELVKATMLTYAGDVVHPAFGGAKKGDL
>Mature_381_residues
GNIVFVAREIAGEETRVAASAETVKKMKSFGFDVVVEAGAGAASRLPDREFEAAGARIGTSADAASADVVLKVRRPSEQE
IAGYKSGAVVIAIMDPYGNDEAIAALASAGLSAFAMELMPRITRAQSMDVLSSQANLAGYQAVIEAAAVYDRAMPMMMTA
AGTVPAAKVFVMGAGVAGLQAIATARRLGAAVSATDVRPAAKEQVASLGAKFIAVEDEEFKAAETAGGYAKEMSADYQAK
QAALVAEHIAKQDIVITTALIPGRAAPRLVSRAMLAAMKPGAVAVDLAVERGGNIEGVVAGEITDVDGVKVIGFANMAGR
VAASASALYAKNLVTFLETMVNKESRSVVVNLDDELVKATMLTYAGDVVHPAFGGAKKGDL

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=381, Percent_Identity=38.0577427821522, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI122939153, Length=381, Percent_Identity=38.0577427821522, Blast_Score=243, Evalue=1e-64,
Organism=Escherichia coli, GI1787887, Length=368, Percent_Identity=43.2065217391304, Blast_Score=292, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17550456, Length=363, Percent_Identity=41.8732782369146, Blast_Score=276, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 39259; Mature: 39127

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNIVFVAREIAGEETRVAASAETVKKMKSFGFDVVVEAGAGAASRLPDREFEAAGARIG
CCCEEEEEHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCCCHHHHCCCCCC
TSADAASADVVLKVRRPSEQEIAGYKSGAVVIAIMDPYGNDEAIAALASAGLSAFAMELM
CCCCCCCCEEEEEECCCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
PRITRAQSMDVLSSQANLAGYQAVIEAAAVYDRAMPMMMTAAGTVPAAKVFVMGAGVAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHH
QAIATARRLGAAVSATDVRPAAKEQVASLGAKFIAVEDEEFKAAETAGGYAKEMSADYQA
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHCCCHHHHHCCHHHH
KQAALVAEHIAKQDIVITTALIPGRAAPRLVSRAMLAAMKPGAVAVDLAVERGGNIEGVV
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEE
AGEITDVDGVKVIGFANMAGRVAASASALYAKNLVTFLETMVNKESRSVVVNLDDELVKA
ECCEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
TMLTYAGDVVHPAFGGAKKGDL
HHHHHCCCHHCCCCCCCCCCCC
>Mature Secondary Structure 
GNIVFVAREIAGEETRVAASAETVKKMKSFGFDVVVEAGAGAASRLPDREFEAAGARIG
CCEEEEEHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCCCHHHHCCCCCC
TSADAASADVVLKVRRPSEQEIAGYKSGAVVIAIMDPYGNDEAIAALASAGLSAFAMELM
CCCCCCCCEEEEEECCCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
PRITRAQSMDVLSSQANLAGYQAVIEAAAVYDRAMPMMMTAAGTVPAAKVFVMGAGVAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHH
QAIATARRLGAAVSATDVRPAAKEQVASLGAKFIAVEDEEFKAAETAGGYAKEMSADYQA
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHCCCHHHHHCCHHHH
KQAALVAEHIAKQDIVITTALIPGRAAPRLVSRAMLAAMKPGAVAVDLAVERGGNIEGVV
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEE
AGEITDVDGVKVIGFANMAGRVAASASALYAKNLVTFLETMVNKESRSVVVNLDDELVKA
ECCEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
TMLTYAGDVVHPAFGGAKKGDL
HHHHHCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]