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The map label for this gene is cheB2 [H]

Identifier: 209550833

GI number: 209550833

Start: 3346256

End: 3347353

Strand: Reverse

Name: cheB2 [H]

Synonym: Rleg2_3257

Alternate gene names: 209550833

Gene position: 3347353-3346256 (Counterclockwise)

Preceding gene: 209550834

Following gene: 209550832

Centisome position: 73.76

GC content: 64.85

Gene sequence:

>1098_bases
ATGGCTAAGAAAATCCGCGTTCTCATCATCGACGATTCCGCCAGCATCCGTCAGACACTGACGCATGTGCTGGAGCAGGA
TCCCGATATCGAGATCATGGCGGTGGCGTCCGACCCCTTCGTGGCGGCGCGCAAGCTGCTGGAGGAAGTCCCCGACGTCA
TCACGCTCGACGTCGAGATGCCGCGCATGGACGGCATCACCTTCCTGCGCAAACTGATGGCGCAGCGGCCGATCCCTGTC
GTGATGTGCTCGTCGCTGACGGAAGCCGGTTCGCAAACGCTGATCCAGGCGCTGGAGGCCGGCGCCGTCGACGTCATTTT
GAAATCGAAGATCGGGGCCGCCGACAGTCTTTCCGATGATGCGATACGCATTCGCGAAATCGTCAAGAGCGCCTCGCATG
CGCGGCTTACCACCGTCCGCCGCGCTGCCGGAACCATCCGCTCGGCTTCGGCCGACGGACCGACCAAGAAGCTGACTGCA
GATGCGATGCTGCCGCCGCCGACGGGGCGGGCGATGGCGAAGACGACCGAGATGGTTGTCTGCGTCGGCGCCTCCACCGG
CGGCACCGAAGCGTTGCGCGAGTTCCTGGAGGAACTGCCGGCCAATGCGCCCGGCATGGTGATCGTCCAGCATATGCCGG
AAAAATTCACCGACGCCTTCGCCAAGCGGCTGAACGGGCTCTGCGAGGTCGAGGTCAAGGAGGCCGTCGATGGCGACCCG
GTGCTGCGCGGGCATGTGCTCATTGCGCCCGGCGACAAGCACACGCTGCTCGAACGCCAGGGCGCGCGCTATTATGTGAG
CGTGAAGACCGGGCCGCTGGTGTCGCGGCACCGGCCTTCCGTCGACGTGCTCTTCCGGTCGGCGGCGCGCTCGGCCGGCT
CGAACGCCATGGGGATCATCATGACCGGCATGGGCGATGACGGCGCGCGCGGTATGCTGGAAATGCATCAGGCGGGCGCC
TATACGGTGGCGCAGGACGAGGCGAGTTCGGTCGTTTTCGGCATGCCGAAGGAGGCGATCGCCAAAGGCGGTGTCGACCG
CATTCTGCCGCTCGACCAGATCGCTCGCGAAGTGATGATGACGCAGCAGAAGTTCTGA

Upstream 100 bases:

>100_bases
GGAGGGTACATGTTCATCGGCCATTCCGAATCGATCACCGGTTTCGATCTGCCGTTGAAGCAGGTCTCGAACACGGTGTT
TCAGCGCATTTAGAGGCGTC

Downstream 100 bases:

>100_bases
GACTGTTTGTCCGATAGTCCAGTATCAATCCCGGTTTCCGGCACAGGATGGCAGCTATGCGCGCCGGCCCTTGAAATTTC
CCTGATTTTGTGGTCTTGAG

Product: response regulator receiver modulated CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MAKKIRVLIIDDSASIRQTLTHVLEQDPDIEIMAVASDPFVAARKLLEEVPDVITLDVEMPRMDGITFLRKLMAQRPIPV
VMCSSLTEAGSQTLIQALEAGAVDVILKSKIGAADSLSDDAIRIREIVKSASHARLTTVRRAAGTIRSASADGPTKKLTA
DAMLPPPTGRAMAKTTEMVVCVGASTGGTEALREFLEELPANAPGMVIVQHMPEKFTDAFAKRLNGLCEVEVKEAVDGDP
VLRGHVLIAPGDKHTLLERQGARYYVSVKTGPLVSRHRPSVDVLFRSAARSAGSNAMGIIMTGMGDDGARGMLEMHQAGA
YTVAQDEASSVVFGMPKEAIAKGGVDRILPLDQIAREVMMTQQKF

Sequences:

>Translated_365_residues
MAKKIRVLIIDDSASIRQTLTHVLEQDPDIEIMAVASDPFVAARKLLEEVPDVITLDVEMPRMDGITFLRKLMAQRPIPV
VMCSSLTEAGSQTLIQALEAGAVDVILKSKIGAADSLSDDAIRIREIVKSASHARLTTVRRAAGTIRSASADGPTKKLTA
DAMLPPPTGRAMAKTTEMVVCVGASTGGTEALREFLEELPANAPGMVIVQHMPEKFTDAFAKRLNGLCEVEVKEAVDGDP
VLRGHVLIAPGDKHTLLERQGARYYVSVKTGPLVSRHRPSVDVLFRSAARSAGSNAMGIIMTGMGDDGARGMLEMHQAGA
YTVAQDEASSVVFGMPKEAIAKGGVDRILPLDQIAREVMMTQQKF
>Mature_364_residues
AKKIRVLIIDDSASIRQTLTHVLEQDPDIEIMAVASDPFVAARKLLEEVPDVITLDVEMPRMDGITFLRKLMAQRPIPVV
MCSSLTEAGSQTLIQALEAGAVDVILKSKIGAADSLSDDAIRIREIVKSASHARLTTVRRAAGTIRSASADGPTKKLTAD
AMLPPPTGRAMAKTTEMVVCVGASTGGTEALREFLEELPANAPGMVIVQHMPEKFTDAFAKRLNGLCEVEVKEAVDGDPV
LRGHVLIAPGDKHTLLERQGARYYVSVKTGPLVSRHRPSVDVLFRSAARSAGSNAMGIIMTGMGDDGARGMLEMHQAGAY
TVAQDEASSVVFGMPKEAIAKGGVDRILPLDQIAREVMMTQQKF

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=357, Percent_Identity=50.9803921568627, Blast_Score=338, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 39135; Mature: 39004

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKIRVLIIDDSASIRQTLTHVLEQDPDIEIMAVASDPFVAARKLLEEVPDVITLDVEM
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECC
PRMDGITFLRKLMAQRPIPVVMCSSLTEAGSQTLIQALEAGAVDVILKSKIGAADSLSDD
CCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCCH
AIRIREIVKSASHARLTTVRRAAGTIRSASADGPTKKLTADAMLPPPTGRAMAKTTEMVV
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCHHHCCCEEEE
CVGASTGGTEALREFLEELPANAPGMVIVQHMPEKFTDAFAKRLNGLCEVEVKEAVDGDP
EEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEHHCCCCCC
VLRGHVLIAPGDKHTLLERQGARYYVSVKTGPLVSRHRPSVDVLFRSAARSAGSNAMGII
EEEEEEEEECCCCHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
MTGMGDDGARGMLEMHQAGAYTVAQDEASSVVFGMPKEAIAKGGVDRILPLDQIAREVMM
EECCCCCCCHHHHHHHHCCCEEEECCCCCCEEECCCHHHHHCCCCHHCCCHHHHHHHHHH
TQQKF
HHCCC
>Mature Secondary Structure 
AKKIRVLIIDDSASIRQTLTHVLEQDPDIEIMAVASDPFVAARKLLEEVPDVITLDVEM
CCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECC
PRMDGITFLRKLMAQRPIPVVMCSSLTEAGSQTLIQALEAGAVDVILKSKIGAADSLSDD
CCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCCH
AIRIREIVKSASHARLTTVRRAAGTIRSASADGPTKKLTADAMLPPPTGRAMAKTTEMVV
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCHHHCCCEEEE
CVGASTGGTEALREFLEELPANAPGMVIVQHMPEKFTDAFAKRLNGLCEVEVKEAVDGDP
EEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEHHCCCCCC
VLRGHVLIAPGDKHTLLERQGARYYVSVKTGPLVSRHRPSVDVLFRSAARSAGSNAMGII
EEEEEEEEECCCCHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
MTGMGDDGARGMLEMHQAGAYTVAQDEASSVVFGMPKEAIAKGGVDRILPLDQIAREVMM
EECCCCCCCHHHHHHHHCCCEEEECCCCCCEEECCCHHHHHCCCCHHCCCHHHHHHHHHH
TQQKF
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA