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The map label for this gene is emrB [H]

Identifier: 209550679

GI number: 209550679

Start: 3169174

End: 3170715

Strand: Reverse

Name: emrB [H]

Synonym: Rleg2_3103

Alternate gene names: 209550679

Gene position: 3170715-3169174 (Counterclockwise)

Preceding gene: 209550680

Following gene: 209550675

Centisome position: 69.87

GC content: 62.45

Gene sequence:

>1542_bases
ATGAGTAACGCTTCCGCTTCCCCGGCGAGCCCGGTAGCCAATCGCGGTGCGATCACGGCCTGCGTCATCCTCGCCGTCAT
CATGCAGGCGCTGGACACGACGATCGCCAACGTGGCGCTGCCCTATATCCAGGGCAGCGTGTCGGCTTCTGCCGACCAGA
TCAACTGGGTCTTAACCTCCTACATCGTCGCAGCGGCGATCATGACGCCGCCATCGGGCTTCCTTGCCGCCAAGTTCGGC
CGCAAGCGCGTGCTGCTCGTCGCCATCGCCGGCTTCGTGGCGGCCTCCGTTCTCTGCGGCCTGGCGCAATCGCTAAACCA
GATCGTCGCCTTCCGCCTGCTGCAGGGCCTGTTCGGCGCATCGCTGGTGCCGCTTTCCCAGGGCATCCTCCTCGATATTT
ATACGGTCGAGGAACGCGGCTCGGCCATGGCGCTTTTCGGCGTCTCGGTCATGGTCGGACCAGTGCTCGGGCCGGTCATC
GGCGGCTGGCTGACCGACAATATCAGCTGGCGCTGGGTCTTCTACATCAACGTGCCGATCGGCGCGCTCGCCTTTGCCGG
TATCGTCGTCTTCGTCACTGAGACCAAGAGGGATGTGCTCGCCAAGTTGGACTGGTTCGGTTTCGGGATGATGAGCCTGT
TCATCGCCTCGCTGCAGCTCTTCCTCGACCGCGGCGAGCAGCTCGACTGGTTCTCCTCCGGCGAGATCATGCTCGAAGCG
ATGATCTGTGCGGCGGCCTTCTATCTGCTTCTGGTGCACACGCTGACGGCTGAGAAATCCTTCGTCAATCCGAAGCTGTT
CCTCGACCAGAATTTTTCGATCAGCATGATCTTCATCTTCGTGGTCGGCGTCACCTATCTCGCCTCGCTGGCGCTGATGA
CCCCCTATCTGCAGACGTTGATGGGCTATCCCGTGATCACCGCCGGCATCGTCATGGGGCCGCGCGGGCTCGGCACCATG
CTCTGCATGTTCATTGTCGGGCGGCTGATCGGTAAGGTCGATACGCGCGTGCTACTGGTGCTCGGTCTCGGCCTGACTGC
CTGGGCGATGTACGACATGACCGGCTGGACGCCGGATGTTTCGCAATGGACGATCATCTCGGTCGGCTTCATCCAGGGCG
CCGGCCTGGGCTTTCTCTTCGTGCCGCTGACGACGATCGCTTTCGCGACGCTGCCCGCCCATATGCGCGGCGACGGCACC
GGCCTCTACAATCTGTCGCGCAACATCGGCTCGGCGGTCGGCATCTCGATCGTCTCGGCGCTGATCGTCGAGAATACCCA
GAGCAATCATGAATCGATCGCGGCCTATGTGACGCCGTTCAATCATGCCTTCAGCGCCGTGGCGGCGCAGGGGCTCAGTC
CGACGACAGCCGCCGGGCGCGCTTCGCTCAACGAGCTCATTACGCTGCAATCGACGATCATCGCCTATATGGACGATTTC
AAGCTCCTGATGCTGATGTCGCTCGCGGTCATTCCGCTGGCGCTGCTGCTGCGTAAGCCGAAGGCGGCGCCCGCCGTCGA
CCACAGTGCGGTGATGGAATAA

Upstream 100 bases:

>100_bases
CGTGTCGGCATGAGCACGGTCGTCGATGTCGAAACCGGCCATGCCCGCGGCCTGCCCGATTTCGTCAATAAACTTCTGGG
CAGACCCCAGGACAAGGGTC

Downstream 100 bases:

>100_bases
TCAGCGAAACGGCTTGCTGAGATAACGCGATCCCTGAATGCCGAAGCGCCATGGCACTGCGGCATTTTTGGTGATGCCGA
TGCGTTTTCCCGAGACGACA

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSNASASPASPVANRGAITACVILAVIMQALDTTIANVALPYIQGSVSASADQINWVLTSYIVAAAIMTPPSGFLAAKFG
RKRVLLVAIAGFVAASVLCGLAQSLNQIVAFRLLQGLFGASLVPLSQGILLDIYTVEERGSAMALFGVSVMVGPVLGPVI
GGWLTDNISWRWVFYINVPIGALAFAGIVVFVTETKRDVLAKLDWFGFGMMSLFIASLQLFLDRGEQLDWFSSGEIMLEA
MICAAAFYLLLVHTLTAEKSFVNPKLFLDQNFSISMIFIFVVGVTYLASLALMTPYLQTLMGYPVITAGIVMGPRGLGTM
LCMFIVGRLIGKVDTRVLLVLGLGLTAWAMYDMTGWTPDVSQWTIISVGFIQGAGLGFLFVPLTTIAFATLPAHMRGDGT
GLYNLSRNIGSAVGISIVSALIVENTQSNHESIAAYVTPFNHAFSAVAAQGLSPTTAAGRASLNELITLQSTIIAYMDDF
KLLMLMSLAVIPLALLLRKPKAAPAVDHSAVME

Sequences:

>Translated_513_residues
MSNASASPASPVANRGAITACVILAVIMQALDTTIANVALPYIQGSVSASADQINWVLTSYIVAAAIMTPPSGFLAAKFG
RKRVLLVAIAGFVAASVLCGLAQSLNQIVAFRLLQGLFGASLVPLSQGILLDIYTVEERGSAMALFGVSVMVGPVLGPVI
GGWLTDNISWRWVFYINVPIGALAFAGIVVFVTETKRDVLAKLDWFGFGMMSLFIASLQLFLDRGEQLDWFSSGEIMLEA
MICAAAFYLLLVHTLTAEKSFVNPKLFLDQNFSISMIFIFVVGVTYLASLALMTPYLQTLMGYPVITAGIVMGPRGLGTM
LCMFIVGRLIGKVDTRVLLVLGLGLTAWAMYDMTGWTPDVSQWTIISVGFIQGAGLGFLFVPLTTIAFATLPAHMRGDGT
GLYNLSRNIGSAVGISIVSALIVENTQSNHESIAAYVTPFNHAFSAVAAQGLSPTTAAGRASLNELITLQSTIIAYMDDF
KLLMLMSLAVIPLALLLRKPKAAPAVDHSAVME
>Mature_512_residues
SNASASPASPVANRGAITACVILAVIMQALDTTIANVALPYIQGSVSASADQINWVLTSYIVAAAIMTPPSGFLAAKFGR
KRVLLVAIAGFVAASVLCGLAQSLNQIVAFRLLQGLFGASLVPLSQGILLDIYTVEERGSAMALFGVSVMVGPVLGPVIG
GWLTDNISWRWVFYINVPIGALAFAGIVVFVTETKRDVLAKLDWFGFGMMSLFIASLQLFLDRGEQLDWFSSGEIMLEAM
ICAAAFYLLLVHTLTAEKSFVNPKLFLDQNFSISMIFIFVVGVTYLASLALMTPYLQTLMGYPVITAGIVMGPRGLGTML
CMFIVGRLIGKVDTRVLLVLGLGLTAWAMYDMTGWTPDVSQWTIISVGFIQGAGLGFLFVPLTTIAFATLPAHMRGDGTG
LYNLSRNIGSAVGISIVSALIVENTQSNHESIAAYVTPFNHAFSAVAAQGLSPTTAAGRASLNELITLQSTIIAYMDDFK
LLMLMSLAVIPLALLLRKPKAAPAVDHSAVME

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=491, Percent_Identity=33.8085539714868, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1788710, Length=509, Percent_Identity=31.6306483300589, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1790195, Length=414, Percent_Identity=28.5024154589372, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788392, Length=418, Percent_Identity=31.1004784688995, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081983, Length=432, Percent_Identity=25.2314814814815, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788509, Length=137, Percent_Identity=31.3868613138686, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790146, Length=155, Percent_Identity=30.3225806451613, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=303, Percent_Identity=26.0726072607261, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321663, Length=135, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=133, Percent_Identity=31.5789473684211, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323000, Length=126, Percent_Identity=31.7460317460317, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324847, Length=139, Percent_Identity=29.4964028776978, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54587; Mature: 54456

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNASASPASPVANRGAITACVILAVIMQALDTTIANVALPYIQGSVSASADQINWVLTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
YIVAAAIMTPPSGFLAAKFGRKRVLLVAIAGFVAASVLCGLAQSLNQIVAFRLLQGLFGA
HHHHHHHCCCCCCHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLVPLSQGILLDIYTVEERGSAMALFGVSVMVGPVLGPVIGGWLTDNISWRWVFYINVPI
CHHHCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEEECCH
GALAFAGIVVFVTETKRDVLAKLDWFGFGMMSLFIASLQLFLDRGEQLDWFSSGEIMLEA
HHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
MICAAAFYLLLVHTLTAEKSFVNPKLFLDQNFSISMIFIFVVGVTYLASLALMTPYLQTL
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGYPVITAGIVMGPRGLGTMLCMFIVGRLIGKVDTRVLLVLGLGLTAWAMYDMTGWTPDV
HCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SQWTIISVGFIQGAGLGFLFVPLTTIAFATLPAHMRGDGTGLYNLSRNIGSAVGISIVSA
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCCCEEEHHHHHHHHHHHHHHHHH
LIVENTQSNHESIAAYVTPFNHAFSAVAAQGLSPTTAAGRASLNELITLQSTIIAYMDDF
HHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KLLMLMSLAVIPLALLLRKPKAAPAVDHSAVME
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SNASASPASPVANRGAITACVILAVIMQALDTTIANVALPYIQGSVSASADQINWVLTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
YIVAAAIMTPPSGFLAAKFGRKRVLLVAIAGFVAASVLCGLAQSLNQIVAFRLLQGLFGA
HHHHHHHCCCCCCHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLVPLSQGILLDIYTVEERGSAMALFGVSVMVGPVLGPVIGGWLTDNISWRWVFYINVPI
CHHHCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEEECCH
GALAFAGIVVFVTETKRDVLAKLDWFGFGMMSLFIASLQLFLDRGEQLDWFSSGEIMLEA
HHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
MICAAAFYLLLVHTLTAEKSFVNPKLFLDQNFSISMIFIFVVGVTYLASLALMTPYLQTL
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGYPVITAGIVMGPRGLGTMLCMFIVGRLIGKVDTRVLLVLGLGLTAWAMYDMTGWTPDV
HCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SQWTIISVGFIQGAGLGFLFVPLTTIAFATLPAHMRGDGTGLYNLSRNIGSAVGISIVSA
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCCCEEEHHHHHHHHHHHHHHHHH
LIVENTQSNHESIAAYVTPFNHAFSAVAAQGLSPTTAAGRASLNELITLQSTIIAYMDDF
HHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KLLMLMSLAVIPLALLLRKPKAAPAVDHSAVME
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]