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The map label for this gene is purA [H]

Identifier: 209550665

GI number: 209550665

Start: 3151415

End: 3152713

Strand: Reverse

Name: purA [H]

Synonym: Rleg2_3089

Alternate gene names: 209550665

Gene position: 3152713-3151415 (Counterclockwise)

Preceding gene: 209550667

Following gene: 209550664

Centisome position: 69.47

GC content: 63.51

Gene sequence:

>1299_bases
ATGACGAACGTAGTCGTGGTCGGTTCGCAATGGGGTGACGAAGGCAAGGGCAAGATTGTCGACTGGCTTTCGGAGCGCGC
GGATATCGTTGTGCGCTATCAGGGCGGACATAATGCCGGCCATACGCTCGTCATCGACGGCACCAGTTACAAGCTGTCGC
TGCTGCCCTCCGGCGTCGTGCGCCCGGGCAAAATGGCGGTGATCGGCAACGGCGTCGTCGTCGATCCGCATGCGCTGATC
GCCGAGATCGGTCGGCTGGAAGCCCAGGGCGTGACAGTGACGCCCGACAATCTGCGTATCGCCGACAATGCGACGCTCAT
TCTGTCGCTGCACCGCGAACTCGACGCGATGCGCGAGGATGCGGCGTCGAACAGCGGCACCAAGATCGGCACGACACGCC
GCGGCATCGGCCCTGCATATGAAGACAAGGTCGGCCGCCGCGCCATCCGGGTGATGGATCTTGCCGATCTCGACAGCCTG
GCCGGCAAGGTCGACCGTATTCTGACGCATCACAATGCGCTTCGCCGCGGCCTCGGCGTCGCCGAAGTCAGCCACCAGAC
GATCATGGACGAACTGACCTCGATCGCCGATCGGGTGCTGCCGTTCCGTGACACCGTCTGGCTTTTCCTCGACAAGGAGC
GCCGCAAGGGCTCCCGCATCCTCTTCGAAGGCGCGCAGGGCAGCCTGCTCGACATCGACCACGGCACCTATCCTTTCGTG
ACCTCGTCGAACACCGTGGCCGGCCAGGCCGCGGCCGGTTCCGGCATGGGGCCGGGCTCGCTCGGCTATATCCTCGGCAT
CACCAAGGCCTATACGACGCGTGTCGGCGAAGGCCCGTTCCCGACGGAGCTGAAGGATGCGATCGGTGAGTTCCTTGGCG
AAAAAGGCCATGAGTTCGGCGTGGTGACCGGGCGCAAGCGGCGTTGCGGCTGGTTCGATGCCGCCCTCGTGCGCCAGTCG
ATCGCCACCAACGGCATCACGGGCATCGCGCTCACCAAGCTCGACGTGCTCGACGGCCTTGAGGAGTTGAAGATCTGCGT
CGGTTACATGCTCGACGGCGAACAGATTGATCATCTTCCCGCAAGCCAGGGAGCGCAAGCTAGGGTCGAACCGGTCTATA
TCACGTTGGAAGGGTGGAAGGAATCGACCGTCGGCGCCCGCAGTTGGGCGGACCTGCCGGCACAGGCGATCAAATATGTT
CGCCAGGTCGAAGAGCTGATCGGCGCGCCTGTCGCGCTGCTTTCCACCAGCCCGGAGCGGGATGACACGATACTTGTGAC
CGATCCGTTTGAGGATTAA

Upstream 100 bases:

>100_bases
ATCACCTGTGACAACAGGGTCGCTTTCCCAAAAACAAAGCTTTATAGATGCGCGGGTTTGAGCAGGTGCGCGGTTTTCCG
CCGGTAACAGGAAGAGTTGG

Downstream 100 bases:

>100_bases
GCTCACGGGTCACTGTGGAGGATGGTTCGGTAGGGTGCCGGACTTCTTGAGAAAGTACTGATGGCGGATTTTATTGCAGT
TATTCGGCGGGCCGTCGACG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTNVVVVGSQWGDEGKGKIVDWLSERADIVVRYQGGHNAGHTLVIDGTSYKLSLLPSGVVRPGKMAVIGNGVVVDPHALI
AEIGRLEAQGVTVTPDNLRIADNATLILSLHRELDAMREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADLDSL
AGKVDRILTHHNALRRGLGVAEVSHQTIMDELTSIADRVLPFRDTVWLFLDKERRKGSRILFEGAQGSLLDIDHGTYPFV
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELKDAIGEFLGEKGHEFGVVTGRKRRCGWFDAALVRQS
IATNGITGIALTKLDVLDGLEELKICVGYMLDGEQIDHLPASQGAQARVEPVYITLEGWKESTVGARSWADLPAQAIKYV
RQVEELIGAPVALLSTSPERDDTILVTDPFED

Sequences:

>Translated_432_residues
MTNVVVVGSQWGDEGKGKIVDWLSERADIVVRYQGGHNAGHTLVIDGTSYKLSLLPSGVVRPGKMAVIGNGVVVDPHALI
AEIGRLEAQGVTVTPDNLRIADNATLILSLHRELDAMREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADLDSL
AGKVDRILTHHNALRRGLGVAEVSHQTIMDELTSIADRVLPFRDTVWLFLDKERRKGSRILFEGAQGSLLDIDHGTYPFV
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELKDAIGEFLGEKGHEFGVVTGRKRRCGWFDAALVRQS
IATNGITGIALTKLDVLDGLEELKICVGYMLDGEQIDHLPASQGAQARVEPVYITLEGWKESTVGARSWADLPAQAIKYV
RQVEELIGAPVALLSTSPERDDTILVTDPFED
>Mature_431_residues
TNVVVVGSQWGDEGKGKIVDWLSERADIVVRYQGGHNAGHTLVIDGTSYKLSLLPSGVVRPGKMAVIGNGVVVDPHALIA
EIGRLEAQGVTVTPDNLRIADNATLILSLHRELDAMREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADLDSLA
GKVDRILTHHNALRRGLGVAEVSHQTIMDELTSIADRVLPFRDTVWLFLDKERRKGSRILFEGAQGSLLDIDHGTYPFVT
SSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELKDAIGEFLGEKGHEFGVVTGRKRRCGWFDAALVRQSI
ATNGITGIALTKLDVLDGLEELKICVGYMLDGEQIDHLPASQGAQARVEPVYITLEGWKESTVGARSWADLPAQAIKYVR
QVEELIGAPVALLSTSPERDDTILVTDPFED

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=423, Percent_Identity=44.2080378250591, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=42.8235294117647, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=42.3857868020305, Blast_Score=316, Evalue=2e-86,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=51.3888888888889, Blast_Score=441, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=43.4883720930233, Blast_Score=348, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=43.4883720930233, Blast_Score=348, Evalue=3e-96,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=40.2777777777778, Blast_Score=325, Evalue=8e-90,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=43.6320754716981, Blast_Score=343, Evalue=1e-94,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46466; Mature: 46335

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVVVVGSQWGDEGKGKIVDWLSERADIVVRYQGGHNAGHTLVIDGTSYKLSLLPSGVV
CCCEEEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCEEEEECCCCCC
RPGKMAVIGNGVVVDPHALIAEIGRLEAQGVTVTPDNLRIADNATLILSLHRELDAMRED
CCCCEEEEECCEEECCHHHHHHHHCCCCCCEEECCCCEEEECCCEEEEEEHHHHHHHHHH
AASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADLDSLAGKVDRILTHHNALRRGLGV
HCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AEVSHQTIMDELTSIADRVLPFRDTVWLFLDKERRKGSRILFEGAQGSLLDIDHGTYPFV
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCEE
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELKDAIGEFLGEKGHEFG
ECCCCCCCCCCCCCCCCCCCCHHHEEHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VVTGRKRRCGWFDAALVRQSIATNGITGIALTKLDVLDGLEELKICVGYMLDGEQIDHLP
EEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHHEECCCHHCCCC
ASQGAQARVEPVYITLEGWKESTVGARSWADLPAQAIKYVRQVEELIGAPVALLSTSPER
CCCCCCCEEEEEEEEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCC
DDTILVTDPFED
CCEEEEECCCCC
>Mature Secondary Structure 
TNVVVVGSQWGDEGKGKIVDWLSERADIVVRYQGGHNAGHTLVIDGTSYKLSLLPSGVV
CCEEEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCEEEEECCCCCC
RPGKMAVIGNGVVVDPHALIAEIGRLEAQGVTVTPDNLRIADNATLILSLHRELDAMRED
CCCCEEEEECCEEECCHHHHHHHHCCCCCCEEECCCCEEEECCCEEEEEEHHHHHHHHHH
AASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADLDSLAGKVDRILTHHNALRRGLGV
HCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AEVSHQTIMDELTSIADRVLPFRDTVWLFLDKERRKGSRILFEGAQGSLLDIDHGTYPFV
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCEE
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELKDAIGEFLGEKGHEFG
ECCCCCCCCCCCCCCCCCCCCHHHEEHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VVTGRKRRCGWFDAALVRQSIATNGITGIALTKLDVLDGLEELKICVGYMLDGEQIDHLP
EEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHHEECCCHHCCCC
ASQGAQARVEPVYITLEGWKESTVGARSWADLPAQAIKYVRQVEELIGAPVALLSTSPER
CCCCCCCEEEEEEEEEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCC
DDTILVTDPFED
CCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]