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The map label for this gene is yjfH [C]

Identifier: 209550660

GI number: 209550660

Start: 3145349

End: 3146173

Strand: Reverse

Name: yjfH [C]

Synonym: Rleg2_3084

Alternate gene names: 209550660

Gene position: 3146173-3145349 (Counterclockwise)

Preceding gene: 209550661

Following gene: 209550657

Centisome position: 69.33

GC content: 66.42

Gene sequence:

>825_bases
ATGGCCGCCGTCCCGATCGCCATCGACAGCGCCGACGATCCGCGCATCGCCGAATTCCGCAATATTCGCGAGCGGGATCT
GACGGGCCGGGAAAACCGCTTCATCGCCGAAGGCACCGTCGTGCTGCGCATGCTTGCCGAGGCGCATGCGCAAGACCGCG
GCTTTCTCGCCGAGAAGATCCTGCTGCTGCGCAATCGCGTCGACGGCGTTATGCCGATCCTCGAGCGGTTGCCGGCCGAT
GTGCCGGTCTATGTCGCCGAGGCCGACGTGCTTGACGGCGTCGTCGGATTCCATCTGCATCGCGGCGTTCTGGCGCTCGG
CCGGCGGGAGGATCGCGGAGAGGCGGCTCTTCTCGAGGCGCTACCGGAACGGGCGCTGGTGCTCGTCGGTTGCGGCATTT
CCAATCATGACAATGCCGGTTCGATGTTCCGCAATGCGGCCGCCTTCCGGGCGGACGCTTTGCTGCTCGACGAAACCTGC
TGTGATCCGCTCTACCGCAAGGCGCTGCGTGTCTCGGTCGGTTCAGTGCTGAGCGTTCCGCACCTGCGCGGCGGCAAGGC
GATGGATGTGCTGCTTTCGCTTGCCGAACGCGGCTTTGCCATCTGGACGCTTTCGCCGCACGGCAAGACCGATATCCGCG
CCATTCCCGCCTCGGCACGCATGGCGCTCGTCATCGGCACCGAGGGGGAGGGGTTGCCGGAGGCGCTGCTGGCACGCTTC
CACAGCGCCCGCATCCCGCAGTCGGAGGATCTCGACAGCCTCAACGCCGCAACGGCAACGGGCATCGCGCTGTTTTCGAT
GGCGTCGGCGATGGGCCGGATCTGA

Upstream 100 bases:

>100_bases
TACGGCGAAACGGCTGAGGGCAAGAGCAAGGCGCTGGTCTTCGACCTGAGGACCGGCCGCACCGTCGCCGACGTCAGCGT
CGCGGGCAATTGAGGCCGGC

Downstream 100 bases:

>100_bases
ATTACGATTTTAAAGCGCGTCGCATCGAACTTGATTGGAACCGCGGCGCACTTCCTAGCGGCATGTATTAGCGCTGGGGA
TCAGCGATGATGGCGGCTGC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MAAVPIAIDSADDPRIAEFRNIRERDLTGRENRFIAEGTVVLRMLAEAHAQDRGFLAEKILLLRNRVDGVMPILERLPAD
VPVYVAEADVLDGVVGFHLHRGVLALGRREDRGEAALLEALPERALVLVGCGISNHDNAGSMFRNAAAFRADALLLDETC
CDPLYRKALRVSVGSVLSVPHLRGGKAMDVLLSLAERGFAIWTLSPHGKTDIRAIPASARMALVIGTEGEGLPEALLARF
HSARIPQSEDLDSLNAATATGIALFSMASAMGRI

Sequences:

>Translated_274_residues
MAAVPIAIDSADDPRIAEFRNIRERDLTGRENRFIAEGTVVLRMLAEAHAQDRGFLAEKILLLRNRVDGVMPILERLPAD
VPVYVAEADVLDGVVGFHLHRGVLALGRREDRGEAALLEALPERALVLVGCGISNHDNAGSMFRNAAAFRADALLLDETC
CDPLYRKALRVSVGSVLSVPHLRGGKAMDVLLSLAERGFAIWTLSPHGKTDIRAIPASARMALVIGTEGEGLPEALLARF
HSARIPQSEDLDSLNAATATGIALFSMASAMGRI
>Mature_273_residues
AAVPIAIDSADDPRIAEFRNIRERDLTGRENRFIAEGTVVLRMLAEAHAQDRGFLAEKILLLRNRVDGVMPILERLPADV
PVYVAEADVLDGVVGFHLHRGVLALGRREDRGEAALLEALPERALVLVGCGISNHDNAGSMFRNAAAFRADALLLDETCC
DPLYRKALRVSVGSVLSVPHLRGGKAMDVLLSLAERGFAIWTLSPHGKTDIRAIPASARMALVIGTEGEGLPEALLARFH
SARIPQSEDLDSLNAATATGIALFSMASAMGRI

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=218, Percent_Identity=27.9816513761468, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29471; Mature: 29340

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVPIAIDSADDPRIAEFRNIRERDLTGRENRFIAEGTVVLRMLAEAHAQDRGFLAEKI
CCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHH
LLLRNRVDGVMPILERLPADVPVYVAEADVLDGVVGFHLHRGVLALGRREDRGEAALLEA
HHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LPERALVLVGCGISNHDNAGSMFRNAAAFRADALLLDETCCDPLYRKALRVSVGSVLSVP
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
HLRGGKAMDVLLSLAERGFAIWTLSPHGKTDIRAIPASARMALVIGTEGEGLPEALLARF
CCCCCHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHH
HSARIPQSEDLDSLNAATATGIALFSMASAMGRI
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAVPIAIDSADDPRIAEFRNIRERDLTGRENRFIAEGTVVLRMLAEAHAQDRGFLAEKI
CCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHH
LLLRNRVDGVMPILERLPADVPVYVAEADVLDGVVGFHLHRGVLALGRREDRGEAALLEA
HHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LPERALVLVGCGISNHDNAGSMFRNAAAFRADALLLDETCCDPLYRKALRVSVGSVLSVP
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
HLRGGKAMDVLLSLAERGFAIWTLSPHGKTDIRAIPASARMALVIGTEGEGLPEALLARF
CCCCCHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHH
HSARIPQSEDLDSLNAATATGIALFSMASAMGRI
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]