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The map label for this gene is livK [C]

Identifier: 209550643

GI number: 209550643

Start: 3129883

End: 3131007

Strand: Reverse

Name: livK [C]

Synonym: Rleg2_3067

Alternate gene names: 209550643

Gene position: 3131007-3129883 (Counterclockwise)

Preceding gene: 209550644

Following gene: 209550640

Centisome position: 69.0

GC content: 59.82

Gene sequence:

>1125_bases
ATGAAGAAGTCTCTTCTGTCGGCAGTGGCTCTGACGGCGATGGTCGCCTTCAGCGGCAACGCATGGGCCGACGTTCTCAT
CGCTGTCGCTGGTCCGCTGACTGGCCCGAACGCTGCGTTCGGCGCTCAGCTCCAGAAGGGCGCCGAGCAGGCGGCCGCAG
ACATCAACGCTGCTGGCGGCATCAATGGCGAGCAGATCAAGCTCGAGCTCGGCGACGACGTCTCCGACCCGAAGCAGGGC
ATTTCGGTCGCCAATAAATTCGTTGCCGACGGCGTCAAGTTCGTCATCGGCCACTTCAACTCGGGCGTTTCGATCCCGGC
TTCGGAAGTCTATGCCGAAAACGGCATCCTCGAAATCACCCCGGCTGCTACGAACCCGAAGTTTACGGAACGCGGTCTCT
GGAACACGTTCCGTACCTGCGGTCGTGACGACCAGCAGGGCGCCATCGCCGGCAAGTATCTCGCCGATCACTTCAAGGAC
GCCAAGATCGCCGTCGTTCACGACAAGACTCCTTACGGTCAGGGTCTTGCCGACGAAACCAAGAAGGCGATGAATGCTGC
CGGTCTGACGGAAGTCATGTATGAAGGCATCAACGTCGGCGACAAGGACTTCTCGGCCCTCATCGCCAAGATGAAGGAAG
CCGGCGTCTCGATCATCTATTGGGGCGGTCTGCACACCGAAGCCGGTCTCATCATCCGTCAGGCCGCCGATCAGGGCCTG
AAGGCAACGCTGGTTTCGGGCGATGGTATCGTTTCGAACGAACTGGCCTCGATCGCTGGCGACGCCGTTGCCGGCACCCT
GAACACCTTCGGTCCTGATCCGACACTGAACCCGGCCAACAAGGAACTCGTCGAAAAGTTCAAGGCTGCCGGTTTCAACC
CGGAAGCCTACACGCTCTATTCCTACGCTGCGATGCAGGCGATCGCCGGTGCCGCCAAGGCTGCCGGTTCGGTCGATCCT
GAAGCCGTCGCCACGGCCATGAAGGAAAAGGGTCCGTTCACGACGGTTCTCGGCGACATTTCGTTCGACGAAAAGGGTGA
CCCGAAGATTCCTGGTTACATCATGTACGAATGGAAGAAGGGCGCAGACGGCAAGTACACCTACGTGCCGCAGGAGACTA
AGTAA

Upstream 100 bases:

>100_bases
TGAATTGCCTTCAGAGTGGAACAGCTTTCCTGGCGCAGTCAATGGTGGGTAGTGCGCTGGGCTTTGGACCGGAACCCGCC
CAAAAATTGGGAGTAACAAT

Downstream 100 bases:

>100_bases
GCTTAGCTTGCTTATTGGTATCATGATCTGATGGAAGCCCGGTTTTGGCCGGGCTTCTTTCGTTCTGTGGGGAACTGAAT
TTCCGTCCGTCTCCGTGCCG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKKSLLSAVALTAMVAFSGNAWADVLIAVAGPLTGPNAAFGAQLQKGAEQAAADINAAGGINGEQIKLELGDDVSDPKQG
ISVANKFVADGVKFVIGHFNSGVSIPASEVYAENGILEITPAATNPKFTERGLWNTFRTCGRDDQQGAIAGKYLADHFKD
AKIAVVHDKTPYGQGLADETKKAMNAAGLTEVMYEGINVGDKDFSALIAKMKEAGVSIIYWGGLHTEAGLIIRQAADQGL
KATLVSGDGIVSNELASIAGDAVAGTLNTFGPDPTLNPANKELVEKFKAAGFNPEAYTLYSYAAMQAIAGAAKAAGSVDP
EAVATAMKEKGPFTTVLGDISFDEKGDPKIPGYIMYEWKKGADGKYTYVPQETK

Sequences:

>Translated_374_residues
MKKSLLSAVALTAMVAFSGNAWADVLIAVAGPLTGPNAAFGAQLQKGAEQAAADINAAGGINGEQIKLELGDDVSDPKQG
ISVANKFVADGVKFVIGHFNSGVSIPASEVYAENGILEITPAATNPKFTERGLWNTFRTCGRDDQQGAIAGKYLADHFKD
AKIAVVHDKTPYGQGLADETKKAMNAAGLTEVMYEGINVGDKDFSALIAKMKEAGVSIIYWGGLHTEAGLIIRQAADQGL
KATLVSGDGIVSNELASIAGDAVAGTLNTFGPDPTLNPANKELVEKFKAAGFNPEAYTLYSYAAMQAIAGAAKAAGSVDP
EAVATAMKEKGPFTTVLGDISFDEKGDPKIPGYIMYEWKKGADGKYTYVPQETK
>Mature_374_residues
MKKSLLSAVALTAMVAFSGNAWADVLIAVAGPLTGPNAAFGAQLQKGAEQAAADINAAGGINGEQIKLELGDDVSDPKQG
ISVANKFVADGVKFVIGHFNSGVSIPASEVYAENGILEITPAATNPKFTERGLWNTFRTCGRDDQQGAIAGKYLADHFKD
AKIAVVHDKTPYGQGLADETKKAMNAAGLTEVMYEGINVGDKDFSALIAKMKEAGVSIIYWGGLHTEAGLIIRQAADQGL
KATLVSGDGIVSNELASIAGDAVAGTLNTFGPDPTLNPANKELVEKFKAAGFNPEAYTLYSYAAMQAIAGAAKAAGSVDP
EAVATAMKEKGPFTTVLGDISFDEKGDPKIPGYIMYEWKKGADGKYTYVPQETK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=369, Percent_Identity=41.1924119241192, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI48994941, Length=346, Percent_Identity=41.907514450867, Blast_Score=261, Evalue=7e-71,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39032; Mature: 39032

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLLSAVALTAMVAFSGNAWADVLIAVAGPLTGPNAAFGAQLQKGAEQAAADINAAGG
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
INGEQIKLELGDDVSDPKQGISVANKFVADGVKFVIGHFNSGVSIPASEVYAENGILEIT
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHCCCCEEEE
PAATNPKFTERGLWNTFRTCGRDDQQGAIAGKYLADHFKDAKIAVVHDKTPYGQGLADET
CCCCCCCHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
KKAMNAAGLTEVMYEGINVGDKDFSALIAKMKEAGVSIIYWGGLHTEAGLIIRQAADQGL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCC
KATLVSGDGIVSNELASIAGDAVAGTLNTFGPDPTLNPANKELVEKFKAAGFNPEAYTLY
EEEEECCCCCHHHHHHHHHCCHHHCCHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHH
SYAAMQAIAGAAKAAGSVDPEAVATAMKEKGPFTTVLGDISFDEKGDPKIPGYIMYEWKK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEEEEC
GADGKYTYVPQETK
CCCCCEEECCCCCC
>Mature Secondary Structure
MKKSLLSAVALTAMVAFSGNAWADVLIAVAGPLTGPNAAFGAQLQKGAEQAAADINAAGG
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
INGEQIKLELGDDVSDPKQGISVANKFVADGVKFVIGHFNSGVSIPASEVYAENGILEIT
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHCCCCEEEE
PAATNPKFTERGLWNTFRTCGRDDQQGAIAGKYLADHFKDAKIAVVHDKTPYGQGLADET
CCCCCCCHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
KKAMNAAGLTEVMYEGINVGDKDFSALIAKMKEAGVSIIYWGGLHTEAGLIIRQAADQGL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCC
KATLVSGDGIVSNELASIAGDAVAGTLNTFGPDPTLNPANKELVEKFKAAGFNPEAYTLY
EEEEECCCCCHHHHHHHHHCCHHHCCHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHH
SYAAMQAIAGAAKAAGSVDPEAVATAMKEKGPFTTVLGDISFDEKGDPKIPGYIMYEWKK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEEEEC
GADGKYTYVPQETK
CCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA