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The map label for this gene is ilvD [H]

Identifier: 209550486

GI number: 209550486

Start: 2963606

End: 2965345

Strand: Reverse

Name: ilvD [H]

Synonym: Rleg2_2909

Alternate gene names: 209550486

Gene position: 2965345-2963606 (Counterclockwise)

Preceding gene: 209550487

Following gene: 209550484

Centisome position: 65.35

GC content: 63.16

Gene sequence:

>1740_bases
ATGAAGAAGAAAGCAGAATGGCCGCGCAAGCTGCGCTCGCAGGAATGGTATGGCGGCACCAGCCGCGACGTAATCTACCA
TCGCGGCTGGCTGAAGAACCAGGGTTATCCGCATGACCTGTTCGATGGCCGTCCGGTCATCGGCATCCTGAATACCTGGT
CTGATATGACGCCGTGTAACGGCCATCTGCGCGAACTCGCCGAGAAGGTGAAGGCGGGTGTCTGGGAGGCCGGCGGCTTC
CCGCTCGAGGTGCCGGTGTTCTCGGCATCCGAAAACACTTTCCGCCCGACCGCGATGATGTATCGCAACCTCGCCGCGTT
GGCGGTGGAAGAGGCGATCCGCGGCCAGCCGATGGACGGCTGCGTGCTCTTGGTCGGCTGCGATAAGACCACGCCGTCGC
TGCTCATGGGGGCTGCCTCCTGCGACCTGCCGTCGATCGTCGTCACCGGCGGGCCGATGCTGAACGGCTATTTCCGCGGT
GAGCGTGTCGGTTCGGGCACGCATCTGTGGAAGTTCTCCGAAATGGTGAAGGCCGGCGAGATGACGCAGGCCGAGTTCCT
CGAGGCTGAGGCGTCGATGAGCCGTTCGTCGGGCACCTGCAACACCATGGGCACCGCCTCCACCATGGCCTCCATGGCCG
AGGCGCTCGGCATGGCACTATCAGGCAATGCCGCGATCCCGGGCGTCGATTCCCGCCGCAAGGTCATGGCGCAGCTGACC
GGCCGCCGGATCGTACAGATGGTCAAGGACGACCTGAAGCCCTCCGAGATCATGACGAAACAGGCTTTCGAAAACGCCAT
CCGCACCAATGCGGCGATCGGCGGATCGACCAACGCCGTCATCCACCTGCTTGCGATTGCCGGCCGCGTCGGCATCGATC
TGTCGCTCGACGACTGGGACCGCTGCGGCCGCGACGTTCCCACAATCGTCAACCTGATGCCGTCGGGCAAGTACCTGATG
GAAGAGTTCTTCTATGCCGGCGGCCTGCCGGTGGTGCTGAAGCGCCTCGGCGAGGCGGGCCTGCTGCATAAGGATGCGCT
GACGGTTTCTGGCGAAACCGTCTGGGACGAGGTCAAGGACGTCGTCAACTGGAATGAGGACGTCATCCTGCCGGCCGAAA
AGGCGCTGACCTCTTCGGGCGGCATCGTCGTGCTGCGCGGCAATCTGGCGCCGAAGGGCGCGGTGCTGAAGCCTTCGGCG
GCCTCGCCGCATCTGTTGGTGCACAAGGGCAGGGCAGTCGTGTTCGAGGATATCGACGACTACAAGGCGAAGATCAACGA
CGACAATCTCGACATCGACGAAAACTGCATCATGGTCATGAAGAATTGCGGGCCGAAGGGTTATCCCGGGATGGCCGAAG
TCGGCAACATGGGACTGCCGCCGAAGGTGCTGAAGAAGGGCATCCTCGACATGGTGCGCATTTCCGACGCCCGCATGTCC
GGAACGGCCTACGGCACAGTTGTGCTGCACACCTCGCCGGAAGCGGCGGTCGGCGGGCCGCTCGCGGTCGTGAAAAACGG
CGACATGATTGAGCTCGATGTGCCGAACCGTCGTCTGCATCTCGACATTTCCGACGAGGAATTGGCGCGGCGGCTGGCCG
AATGGCAGCCGAACCACGACCTGCCGACATCGGGTTATGCCTTCCTGCATCAGCAGCATGTCGAAGGGGCCGATACCGGC
GCCGACCTCGACTTCCTCAAGGGATGTCGCGGAAACGCGGTCGGCAAAGACAGCCACTAA

Upstream 100 bases:

>100_bases
TCTACACAACCGTCAAGACCGCCTACACGCTGGCTTGAGACGAAGCGCGATAACCCTGATCGTACGAGGCGGTCAGGGGA
TATCCTGAAGGACAGAGACG

Downstream 100 bases:

>100_bases
CCGGGTGCATCCAAAATTGATAGCTGATGAGGCGGGGCAAAACCCCGCCTCTTTATGTTCGCCGGTTCCGTAGCGGATGA
TGGTGGCGCTCAGACGCTTG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MKKKAEWPRKLRSQEWYGGTSRDVIYHRGWLKNQGYPHDLFDGRPVIGILNTWSDMTPCNGHLRELAEKVKAGVWEAGGF
PLEVPVFSASENTFRPTAMMYRNLAALAVEEAIRGQPMDGCVLLVGCDKTTPSLLMGAASCDLPSIVVTGGPMLNGYFRG
ERVGSGTHLWKFSEMVKAGEMTQAEFLEAEASMSRSSGTCNTMGTASTMASMAEALGMALSGNAAIPGVDSRRKVMAQLT
GRRIVQMVKDDLKPSEIMTKQAFENAIRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWDRCGRDVPTIVNLMPSGKYLM
EEFFYAGGLPVVLKRLGEAGLLHKDALTVSGETVWDEVKDVVNWNEDVILPAEKALTSSGGIVVLRGNLAPKGAVLKPSA
ASPHLLVHKGRAVVFEDIDDYKAKINDDNLDIDENCIMVMKNCGPKGYPGMAEVGNMGLPPKVLKKGILDMVRISDARMS
GTAYGTVVLHTSPEAAVGGPLAVVKNGDMIELDVPNRRLHLDISDEELARRLAEWQPNHDLPTSGYAFLHQQHVEGADTG
ADLDFLKGCRGNAVGKDSH

Sequences:

>Translated_579_residues
MKKKAEWPRKLRSQEWYGGTSRDVIYHRGWLKNQGYPHDLFDGRPVIGILNTWSDMTPCNGHLRELAEKVKAGVWEAGGF
PLEVPVFSASENTFRPTAMMYRNLAALAVEEAIRGQPMDGCVLLVGCDKTTPSLLMGAASCDLPSIVVTGGPMLNGYFRG
ERVGSGTHLWKFSEMVKAGEMTQAEFLEAEASMSRSSGTCNTMGTASTMASMAEALGMALSGNAAIPGVDSRRKVMAQLT
GRRIVQMVKDDLKPSEIMTKQAFENAIRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWDRCGRDVPTIVNLMPSGKYLM
EEFFYAGGLPVVLKRLGEAGLLHKDALTVSGETVWDEVKDVVNWNEDVILPAEKALTSSGGIVVLRGNLAPKGAVLKPSA
ASPHLLVHKGRAVVFEDIDDYKAKINDDNLDIDENCIMVMKNCGPKGYPGMAEVGNMGLPPKVLKKGILDMVRISDARMS
GTAYGTVVLHTSPEAAVGGPLAVVKNGDMIELDVPNRRLHLDISDEELARRLAEWQPNHDLPTSGYAFLHQQHVEGADTG
ADLDFLKGCRGNAVGKDSH
>Mature_579_residues
MKKKAEWPRKLRSQEWYGGTSRDVIYHRGWLKNQGYPHDLFDGRPVIGILNTWSDMTPCNGHLRELAEKVKAGVWEAGGF
PLEVPVFSASENTFRPTAMMYRNLAALAVEEAIRGQPMDGCVLLVGCDKTTPSLLMGAASCDLPSIVVTGGPMLNGYFRG
ERVGSGTHLWKFSEMVKAGEMTQAEFLEAEASMSRSSGTCNTMGTASTMASMAEALGMALSGNAAIPGVDSRRKVMAQLT
GRRIVQMVKDDLKPSEIMTKQAFENAIRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWDRCGRDVPTIVNLMPSGKYLM
EEFFYAGGLPVVLKRLGEAGLLHKDALTVSGETVWDEVKDVVNWNEDVILPAEKALTSSGGIVVLRGNLAPKGAVLKPSA
ASPHLLVHKGRAVVFEDIDDYKAKINDDNLDIDENCIMVMKNCGPKGYPGMAEVGNMGLPPKVLKKGILDMVRISDARMS
GTAYGTVVLHTSPEAAVGGPLAVVKNGDMIELDVPNRRLHLDISDEELARRLAEWQPNHDLPTSGYAFLHQQHVEGADTG
ADLDFLKGCRGNAVGKDSH

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=588, Percent_Identity=30.952380952381, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1788157, Length=516, Percent_Identity=29.8449612403101, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI2367371, Length=550, Percent_Identity=30.5454545454545, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1786464, Length=553, Percent_Identity=32.0072332730561, Blast_Score=185, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6322476, Length=561, Percent_Identity=31.1942959001783, Blast_Score=260, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 62584; Mature: 62584

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKAEWPRKLRSQEWYGGTSRDVIYHRGWLKNQGYPHDLFDGRPVIGILNTWSDMTPCN
CCCCCHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
GHLRELAEKVKAGVWEAGGFPLEVPVFSASENTFRPTAMMYRNLAALAVEEAIRGQPMDG
HHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
CVLLVGCDKTTPSLLMGAASCDLPSIVVTGGPMLNGYFRGERVGSGTHLWKFSEMVKAGE
EEEEEECCCCCHHHHHCCCCCCCCEEEEECCCEECCEECCCCCCCCCCHHHHHHHHHHCC
MTQAEFLEAEASMSRSSGTCNTMGTASTMASMAEALGMALSGNAAIPGVDSRRKVMAQLT
CHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHHH
GRRIVQMVKDDLKPSEIMTKQAFENAIRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEECCCCHH
RCGRDVPTIVNLMPSGKYLMEEFFYAGGLPVVLKRLGEAGLLHKDALTVSGETVWDEVKD
HCCCCCCHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCCEECCCEEECCCHHHHHHHH
VVNWNEDVILPAEKALTSSGGIVVLRGNLAPKGAVLKPSAASPHLLVHKGRAVVFEDIDD
HHCCCCCEEECHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCEEEEECCCEEEEECCCC
YKAKINDDNLDIDENCIMVMKNCGPKGYPGMAEVGNMGLPPKVLKKGILDMVRISDARMS
HHHEECCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
GTAYGTVVLHTSPEAAVGGPLAVVKNGDMIELDVPNRRLHLDISDEELARRLAEWQPNHD
CCEEEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEEEEECCHHHHHHHHHHCCCCCC
LPTSGYAFLHQQHVEGADTGADLDFLKGCRGNAVGKDSH
CCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKKKAEWPRKLRSQEWYGGTSRDVIYHRGWLKNQGYPHDLFDGRPVIGILNTWSDMTPCN
CCCCCHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
GHLRELAEKVKAGVWEAGGFPLEVPVFSASENTFRPTAMMYRNLAALAVEEAIRGQPMDG
HHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
CVLLVGCDKTTPSLLMGAASCDLPSIVVTGGPMLNGYFRGERVGSGTHLWKFSEMVKAGE
EEEEEECCCCCHHHHHCCCCCCCCEEEEECCCEECCEECCCCCCCCCCHHHHHHHHHHCC
MTQAEFLEAEASMSRSSGTCNTMGTASTMASMAEALGMALSGNAAIPGVDSRRKVMAQLT
CHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHHH
GRRIVQMVKDDLKPSEIMTKQAFENAIRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEECCCCHH
RCGRDVPTIVNLMPSGKYLMEEFFYAGGLPVVLKRLGEAGLLHKDALTVSGETVWDEVKD
HCCCCCCHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCCEECCCEEECCCHHHHHHHH
VVNWNEDVILPAEKALTSSGGIVVLRGNLAPKGAVLKPSAASPHLLVHKGRAVVFEDIDD
HHCCCCCEEECHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCEEEEECCCEEEEECCCC
YKAKINDDNLDIDENCIMVMKNCGPKGYPGMAEVGNMGLPPKVLKKGILDMVRISDARMS
HHHEECCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
GTAYGTVVLHTSPEAAVGGPLAVVKNGDMIELDVPNRRLHLDISDEELARRLAEWQPNHD
CCEEEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEEEEECCHHHHHHHHHHCCCCCC
LPTSGYAFLHQQHVEGADTGADLDFLKGCRGNAVGKDSH
CCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]