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The map label for this gene is pucL [H]

Identifier: 209550464

GI number: 209550464

Start: 2936530

End: 2937030

Strand: Reverse

Name: pucL [H]

Synonym: Rleg2_2887

Alternate gene names: 209550464

Gene position: 2937030-2936530 (Counterclockwise)

Preceding gene: 209550465

Following gene: 209550463

Centisome position: 64.72

GC content: 62.87

Gene sequence:

>501_bases
ATGCTGTCGCGCGAGGATTTCGTTTCCCGCTTCGGCGGCGTCTTCGAACATTCGCCTTTCATCGCCGAGCGCGCCTATGA
AGCCGGCAGCGTGACCGAGCCGCTGACGGCATCAGGCGTGCATGCGGCGCTTGCGGCGGTCTTCCGCGCGGCAAGCCGGG
AGGAACGTCTCGGCGTGCTTCGAGCCCACCCCGACCTTGCCGGGCGTCTTGCCATATCCGGCGAACTCACCGAGGACAGC
CGCAAGGAGCAGTCCGGCGCCGGTCTCGACCGGCTGAGCCCTGCCGAGCACGCCCGCTTCACCGAACTCAATTCGGCCTA
TGTCGAAAAATATGGCTTTCCCTTCATCATCGCCGTCAAAGGACTCGGCAAGGAGGATATTCTGGAAGCTTTTGAAACAC
GGATCGGCAACGGCCGGGACGAAGAATTTTCGACCGCGACTGCGCAGGTCGAAAAGATCGCGCTGCTGCGTCTGACCTCG
ATGCTGCCGGAGGCGGAATGA

Upstream 100 bases:

>100_bases
GCTTCATCGAATATGTGCTCAAGCACGACAAGGTGTGGATTCCGCGCCGCATCGAGATTGCCGAGCACTGGCTCAAGCAC
CATCAGCCGGACGCGATCTG

Downstream 100 bases:

>100_bases
AGGAGAGGCGTGCCATCGACTATCTCAATGACATGCAAAAGGCGGCATCCGAAGCGCTGTCCTTCATCGGCGGAATGGAT
GAGGCGGCTTTCGCCGAGGA

Product: OHCU decarboxylase

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MLSREDFVSRFGGVFEHSPFIAERAYEAGSVTEPLTASGVHAALAAVFRAASREERLGVLRAHPDLAGRLAISGELTEDS
RKEQSGAGLDRLSPAEHARFTELNSAYVEKYGFPFIIAVKGLGKEDILEAFETRIGNGRDEEFSTATAQVEKIALLRLTS
MLPEAE

Sequences:

>Translated_166_residues
MLSREDFVSRFGGVFEHSPFIAERAYEAGSVTEPLTASGVHAALAAVFRAASREERLGVLRAHPDLAGRLAISGELTEDS
RKEQSGAGLDRLSPAEHARFTELNSAYVEKYGFPFIIAVKGLGKEDILEAFETRIGNGRDEEFSTATAQVEKIALLRLTS
MLPEAE
>Mature_166_residues
MLSREDFVSRFGGVFEHSPFIAERAYEAGSVTEPLTASGVHAALAAVFRAASREERLGVLRAHPDLAGRLAISGELTEDS
RKEQSGAGLDRLSPAEHARFTELNSAYVEKYGFPFIIAVKGLGKEDILEAFETRIGNGRDEEFSTATAQVEKIALLRLTS
MLPEAE

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG3195

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=157, Percent_Identity=33.7579617834395, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 18069; Mature: 18069

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSREDFVSRFGGVFEHSPFIAERAYEAGSVTEPLTASGVHAALAAVFRAASREERLGVL
CCCHHHHHHHHCCHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RAHPDLAGRLAISGELTEDSRKEQSGAGLDRLSPAEHARFTELNSAYVEKYGFPFIIAVK
HCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
GLGKEDILEAFETRIGNGRDEEFSTATAQVEKIALLRLTSMLPEAE
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSREDFVSRFGGVFEHSPFIAERAYEAGSVTEPLTASGVHAALAAVFRAASREERLGVL
CCCHHHHHHHHCCHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RAHPDLAGRLAISGELTEDSRKEQSGAGLDRLSPAEHARFTELNSAYVEKYGFPFIIAVK
HCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
GLGKEDILEAFETRIGNGRDEEFSTATAQVEKIALLRLTSMLPEAE
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]