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The map label for this gene is metF [H]

Identifier: 209550198

GI number: 209550198

Start: 2661618

End: 2662520

Strand: Reverse

Name: metF [H]

Synonym: Rleg2_2618

Alternate gene names: 209550198

Gene position: 2662520-2661618 (Counterclockwise)

Preceding gene: 209550199

Following gene: 209550192

Centisome position: 58.67

GC content: 64.23

Gene sequence:

>903_bases
ATGGCTTTGAATAACGAGGCACGCGGCCGCAACATCGGGATCTCCTTCGAATTCTTCCCGCCGAAATCGGAGGAGATGGA
AGGTCAGCTCTGGGATACGGTCAGCAAGCTGCAGGACTGGGACCCGGATTTCGTCTCGGTGACTTATGGCGCCGGCGGCA
CCACCAAGGCGCCGACGCTGACTGCCGTCACGCGTTTCCTGTCGCAGACGCCGCTCGCCACCGCTTCGCACCTGACCTGC
GTCGGCGCGACGAAGGAAGAGACGCACCAGATGATCGAGACGTTCCGCAAAGTCGGCGTCACGCATTTCGTAGCGTTGCG
CGGCGATGCGCCCGGCGGCGCCGGCGCGCCCTACCAGCCGCATCCCGGCGGTTATGCCAATGCGGCCGAGCTGGTGGCCG
GCCTCAAGGCGGTCGGTGATTTCGAGATTTCGGTTTCCGCCTATCCGGAAAAGCATCCGGAAAGCCGTGACACGGCCGCC
GATATCGACATGCTGAAGCGCAAGGCCGATAACGGCGCCGACCGGGCGCTGACACAGTTCTTCTTCGACAACGACAATTT
CGAGCGCTACCTGGAGAAGGTGCGCGCTGCCGGGGTCTCGATCCCCGTTGTGCCCGGTATTATGCCGGTCCAGAACCTGA
CGCAGCTGAAGCGTTTCGCCGGCGCCTGCGGCACGATCATCCCGGCCTTTCTCGATGAGCGCTTCGCCGGCTTCGACGAC
AAGCCCGAGGAGCGGGCGAAGGTCGCAGCCGATGTCGCCGCCGAACAGATCGAGGATCTCGTCCGGCGCGGCATCAATGA
GTTCCATCTCTACACGATGAACCGCGCGCCGCTGGTTTCCGCCGTGCTCGACAATCTCGGCTTTTCCCGCCGGGCGGCAA
AAAGTGCGGGCGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
GGGTTGACGGTCACCGTCTGGCTCGCGCGCGATCCGAGGCGCCTGATGGCTTCGCAACTAAGCGAAGGCGCCGAACCTAC
ATTTGCCGGGAGAGTATGAC

Downstream 100 bases:

>100_bases
TTTATACGGCCCAGAAAGGTGAAAAAGCGCATGTCGGGCGGGACATGCGCTTTTTCATTGGCGGATTGATCAGGGGAGTT
CAGGTACTACCAGTCATTCC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MALNNEARGRNIGISFEFFPPKSEEMEGQLWDTVSKLQDWDPDFVSVTYGAGGTTKAPTLTAVTRFLSQTPLATASHLTC
VGATKEETHQMIETFRKVGVTHFVALRGDAPGGAGAPYQPHPGGYANAAELVAGLKAVGDFEISVSAYPEKHPESRDTAA
DIDMLKRKADNGADRALTQFFFDNDNFERYLEKVRAAGVSIPVVPGIMPVQNLTQLKRFAGACGTIIPAFLDERFAGFDD
KPEERAKVAADVAAEQIEDLVRRGINEFHLYTMNRAPLVSAVLDNLGFSRRAAKSAGAAA

Sequences:

>Translated_300_residues
MALNNEARGRNIGISFEFFPPKSEEMEGQLWDTVSKLQDWDPDFVSVTYGAGGTTKAPTLTAVTRFLSQTPLATASHLTC
VGATKEETHQMIETFRKVGVTHFVALRGDAPGGAGAPYQPHPGGYANAAELVAGLKAVGDFEISVSAYPEKHPESRDTAA
DIDMLKRKADNGADRALTQFFFDNDNFERYLEKVRAAGVSIPVVPGIMPVQNLTQLKRFAGACGTIIPAFLDERFAGFDD
KPEERAKVAADVAAEQIEDLVRRGINEFHLYTMNRAPLVSAVLDNLGFSRRAAKSAGAAA
>Mature_299_residues
ALNNEARGRNIGISFEFFPPKSEEMEGQLWDTVSKLQDWDPDFVSVTYGAGGTTKAPTLTAVTRFLSQTPLATASHLTCV
GATKEETHQMIETFRKVGVTHFVALRGDAPGGAGAPYQPHPGGYANAAELVAGLKAVGDFEISVSAYPEKHPESRDTAAD
IDMLKRKADNGADRALTQFFFDNDNFERYLEKVRAAGVSIPVVPGIMPVQNLTQLKRFAGACGTIIPAFLDERFAGFDDK
PEERAKVAADVAAEQIEDLVRRGINEFHLYTMNRAPLVSAVLDNLGFSRRAAKSAGAAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=282, Percent_Identity=28.0141843971631, Blast_Score=128, Evalue=7e-30,
Organism=Escherichia coli, GI1790377, Length=289, Percent_Identity=42.9065743944637, Blast_Score=248, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI25147436, Length=286, Percent_Identity=27.972027972028, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25147441, Length=286, Percent_Identity=27.972027972028, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6321313, Length=274, Percent_Identity=32.4817518248175, Blast_Score=138, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6325234, Length=284, Percent_Identity=30.9859154929577, Blast_Score=128, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32437; Mature: 32306

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALNNEARGRNIGISFEFFPPKSEEMEGQLWDTVSKLQDWDPDFVSVTYGAGGTTKAPTL
CCCCCCCCCCEECEEEEECCCCCHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
TAVTRFLSQTPLATASHLTCVGATKEETHQMIETFRKVGVTHFVALRGDAPGGAGAPYQP
HHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCHHEEEEEECCCCCCCCCCCCC
HPGGYANAAELVAGLKAVGDFEISVSAYPEKHPESRDTAADIDMLKRKADNGADRALTQF
CCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
FFDNDNFERYLEKVRAAGVSIPVVPGIMPVQNLTQLKRFAGACGTIIPAFLDERFAGFDD
HCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KPEERAKVAADVAAEQIEDLVRRGINEFHLYTMNRAPLVSAVLDNLGFSRRAAKSAGAAA
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHCCCCCC
>Mature Secondary Structure 
ALNNEARGRNIGISFEFFPPKSEEMEGQLWDTVSKLQDWDPDFVSVTYGAGGTTKAPTL
CCCCCCCCCEECEEEEECCCCCHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
TAVTRFLSQTPLATASHLTCVGATKEETHQMIETFRKVGVTHFVALRGDAPGGAGAPYQP
HHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCHHEEEEEECCCCCCCCCCCCC
HPGGYANAAELVAGLKAVGDFEISVSAYPEKHPESRDTAADIDMLKRKADNGADRALTQF
CCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
FFDNDNFERYLEKVRAAGVSIPVVPGIMPVQNLTQLKRFAGACGTIIPAFLDERFAGFDD
HCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KPEERAKVAADVAAEQIEDLVRRGINEFHLYTMNRAPLVSAVLDNLGFSRRAAKSAGAAA
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA