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The map label for this gene is amiD [H]

Identifier: 209550187

GI number: 209550187

Start: 2649462

End: 2650223

Strand: Reverse

Name: amiD [H]

Synonym: Rleg2_2607

Alternate gene names: 209550187

Gene position: 2650223-2649462 (Counterclockwise)

Preceding gene: 209550188

Following gene: 209550186

Centisome position: 58.4

GC content: 62.6

Gene sequence:

>762_bases
ATGACCTCTTTCGAGGCCGACTGCGGCAGCGCCCGCGTGCAGCCTTCGCCGAACCATGGCGAGCGCGCGGATGGGCGCCG
TCCGGACATGATCCTGTTGCATTATACCGGCATGCCGACGGCTCTCGGTGCGCTCGACTGGCTCTGTCGCGCCGAGAGCC
AGGTTTCCAGCCATTATTTCGTGCATGAGAACGGCGAGGTGGTGCAGCTCGTGCCGGAGGTGCGGCGTGCCTGGCATGCG
GGAAAGAGCAGTTGGCACGGCGAGACGGATATCAATTCGCTGTCGATCGGCATCGAGATCGCCAATGCCGGCCATCCCGG
CGGGCTTCCCGAATACCCCAAAGAGCAGATCGCTGCGGTGATCGAATTGTGTCGCGACTGTGTCAAACGTTGGTCGATCG
CGCCGGAACGGGTCCTTGGGCACTCCGATGTGGCCCCGATCCGCAAGGTCGATCCGGGTGAAAAATTCCCCTGGGCCGAG
CTCCATAGGGCAGGCATCGGGCACTGGGTCGAGCCGGCAACGATCACCGGCGGGCGCTTCTTCCAGCAGGGTGATGCCGG
CCAGCCTGTCGAAGCGCTGCAGTCGATGCTGTCTCTCTATGGTTACGGCACTGAAATCACGGGCCAATTTTCGGAAAAGA
CGGCAGGTGACGTAGAAGCTTTCCAGCGCCATTTCCGGCCTGAACGGGTGGATGGTATCGCCGATTTCTCGACGATCGAC
ACGCTGCACCGGCTGCTGTCGGCGCTGCCGCGTTATTCCTGA

Upstream 100 bases:

>100_bases
TCTCGCGCGGCGTGCCGAAGGAGTTCCACGTCATCGCCAATGAGCGCATGGCGGCGCTGAACGCGGCCTATGCGGCGATC
GAGAAGGAACGCCGCGCCGC

Downstream 100 bases:

>100_bases
GCGAAAGAGACCGATTTCCGGCCGATATTTCCGCGGCTGGCGAGAAAACCGCCTCGCCACATCATCTCCCTATTATCTCC
TCATTGCGATGGTCTAAGAA

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTSFEADCGSARVQPSPNHGERADGRRPDMILLHYTGMPTALGALDWLCRAESQVSSHYFVHENGEVVQLVPEVRRAWHA
GKSSWHGETDINSLSIGIEIANAGHPGGLPEYPKEQIAAVIELCRDCVKRWSIAPERVLGHSDVAPIRKVDPGEKFPWAE
LHRAGIGHWVEPATITGGRFFQQGDAGQPVEALQSMLSLYGYGTEITGQFSEKTAGDVEAFQRHFRPERVDGIADFSTID
TLHRLLSALPRYS

Sequences:

>Translated_253_residues
MTSFEADCGSARVQPSPNHGERADGRRPDMILLHYTGMPTALGALDWLCRAESQVSSHYFVHENGEVVQLVPEVRRAWHA
GKSSWHGETDINSLSIGIEIANAGHPGGLPEYPKEQIAAVIELCRDCVKRWSIAPERVLGHSDVAPIRKVDPGEKFPWAE
LHRAGIGHWVEPATITGGRFFQQGDAGQPVEALQSMLSLYGYGTEITGQFSEKTAGDVEAFQRHFRPERVDGIADFSTID
TLHRLLSALPRYS
>Mature_252_residues
TSFEADCGSARVQPSPNHGERADGRRPDMILLHYTGMPTALGALDWLCRAESQVSSHYFVHENGEVVQLVPEVRRAWHAG
KSSWHGETDINSLSIGIEIANAGHPGGLPEYPKEQIAAVIELCRDCVKRWSIAPERVLGHSDVAPIRKVDPGEKFPWAEL
HRAGIGHWVEPATITGGRFFQQGDAGQPVEALQSMLSLYGYGTEITGQFSEKTAGDVEAFQRHFRPERVDGIADFSTIDT
LHRLLSALPRYS

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=239, Percent_Identity=38.4937238493724, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786300, Length=168, Percent_Identity=32.1428571428571, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 27921; Mature: 27789

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFEADCGSARVQPSPNHGERADGRRPDMILLHYTGMPTALGALDWLCRAESQVSSHYF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCEEE
VHENGEVVQLVPEVRRAWHAGKSSWHGETDINSLSIGIEIANAGHPGGLPEYPKEQIAAV
EECCCCEEHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHH
IELCRDCVKRWSIAPERVLGHSDVAPIRKVDPGEKFPWAELHRAGIGHWVEPATITGGRF
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEECCCCC
FQQGDAGQPVEALQSMLSLYGYGTEITGQFSEKTAGDVEAFQRHFRPERVDGIADFSTID
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHCCHHHHHCHHHHHHHH
TLHRLLSALPRYS
HHHHHHHHCCCCC
>Mature Secondary Structure 
TSFEADCGSARVQPSPNHGERADGRRPDMILLHYTGMPTALGALDWLCRAESQVSSHYF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCEEE
VHENGEVVQLVPEVRRAWHAGKSSWHGETDINSLSIGIEIANAGHPGGLPEYPKEQIAAV
EECCCCEEHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHH
IELCRDCVKRWSIAPERVLGHSDVAPIRKVDPGEKFPWAELHRAGIGHWVEPATITGGRF
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEECCCCC
FQQGDAGQPVEALQSMLSLYGYGTEITGQFSEKTAGDVEAFQRHFRPERVDGIADFSTID
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHCCHHHHHCHHHHHHHH
TLHRLLSALPRYS
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]