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The map label for this gene is ligA [H]

Identifier: 209550159

GI number: 209550159

Start: 2616480

End: 2618636

Strand: Reverse

Name: ligA [H]

Synonym: Rleg2_2579

Alternate gene names: 209550159

Gene position: 2618636-2616480 (Counterclockwise)

Preceding gene: 209550160

Following gene: 209550157

Centisome position: 57.71

GC content: 63.14

Gene sequence:

>2157_bases
ATGTCCACCGAAGGTTCCGCCGTCGACACATTGACGATCGAAGAGGCTGCCGCCGAGCTTGAGCGGCTGGCAAAGGAGAT
CGCGCATCACGACGCGCTTTACCATGGCAAGGACCGGCCGCAGATTTCGGATGCGGATTACGACGCGCTGAAGCGCCGCA
ACGATGCGCTGGAGGTGCAGTTTCCCGAGCTGATCCGCGAGGATAGCCCGTCGCGGCGGGTGGGTGCTGCGCCCTCCGTT
ACCTTTTCGCCCGTCGTCCATGCGCGGCCGATGCTGTCGCTCGACAACACGTTTTCGCAGGAGGACGTGCAGGATTTCGT
CGCCAGCGTCTATCGCTTCCTCGGCCGCCTGCCGGACCAGTCGATCGCCTTTACCGCCGAGCCGAAGATCGACGGTCTGT
CTATGTCGATCCGTTACGAGAACGGCAGGCTGGTGACGGCGGCGACGCGCGGTGACGGCACGACGGGGGAGAATGTCACC
GCCAATATTCGCACTATTGCCGAAATCCCGAACGAGCTGCCAAAGGGCGTGCCCGCGGTGGTGGAGATCCGCGGCGAGGT
CTATATGGCCAAGAGCGACTTTCTGGCGCTGAACCGGCAGATGGAAGCGGAGGGCAAGCAGACCTATGTCAATCCGCGCA
ATACCGCCGCCGGGTCGCTGCGCCAGCTCGACGCCAAGGTGACGGCCAGCCGCAAGCTGAAATTCTTCGCCTATGCCTGG
GGTGAAATGTCGGAAATGCCCGCCGACACCCAGTTCGACATGGTGCAGACCTTCAAGGATTGGGGTTTTCCTGTAAACCC
GCTGATGAAGCGGCTGAATTCGGTCGCCGACATCCTGGCGCATTACGACGAGATCGGTCTGGAACGCCCGGATCTCGACT
ACGACATCGACGGCGTCGTCTACAAGGTCGACAGCCTCGAGCTGCAGGCCCGTCTCGGCTTCCGTTCGCGTTCGCCGCGC
TGGGCGACGGCGCACAAATTTCCGGCCGAACAGGCCTTCACCGAGGTCGAGAAAATCGAGATCCAGGTCGGCCGCACTGG
CGCGCTGACGCCGGTGGCGCGACTGAAGCCGATCACCGTCGGCGGCGTCGTCGTCACCAATGCGACGCTGCACAACGAGG
ACTACATCAAGGGTATCGGCAATAGCGGCGAGCGCATTCGGCCTGAAGATCACGATATCCGCGAGGGCGATACCGTCATC
GTCCAGCGCGCCGGCGACGTCATTCCGCAGATCCTCGACGTGGTGATGGAAAAACGCACGGCCGACGCCCGGTCGTACGA
ATTTCCGAAAACCTGCCCGGTCTGCGGTTCGCATGCGGTGCGCGAGGTCAACGAGAAGACCGGCAAGATGGATTCGGTGC
GCCGCTGCACCGGCGGCTTCATCTGCCGCGCACAGGCGACCGAGCATCTGAAGCACTTCGTCTCGCGCAACGCCTTCGAT
ATCGAGGGGCTCGGATCGAAGCAGATCGACTTCTTCTTCGAACACGAGGACGCGTCGCTGCAGATCCGCACCGCGCCTGA
AATCTTCACGCTGGAGAAGCGCCAGCAGGCTTCGCTGACCAAGCTGGAAAATATCGACGGCTTCGGCAAGGTCAGCGTCG
GCAAGCTTTATACGGCGATCAACGAACGGCGCAGCATTGCACTCCACCGCTTCATCTACGCGCTCGGCATCCGTCATGTC
GGCGAGACCACGGCAAAGCTGCTGGCGCGCTCCTATGGCACCTACGAAGCCTTTGCGATGGCGATGAAGGAGGCGGCACC
GCTTTCCGGCGACGCCTGGAACGATCTCAACGCCATCGAGGGCATCGGCGAGGTGGTGGCGCGCGCCATGGTCGAATTCT
ACAAGGAGCCGCGCAATGTCGAGGTGATCACCCGGCTCATCGAGGAAGTGACGCCCCAGCAGGCCGAACAGCCGGTAACG
ACCGGCAGTCCGGTGGCCGGCAAGACCGTGGTCTTTACCGGCTCGCTGGAAAAATTCACCCGCGACGAGGCGAAGGCGAG
GGCGGAAAGCCTCGGCGCCAAGGTGGCAGGATCGGTCTCCAAGAAGACCGACATCGTCGTCGCCGGCCCCGGCGCCGGCT
CCAAGCTCGATAAGGCGCGCGAACTCGGCGTGCAGACGATGGACGAGGACGAGTGGCTGGCGCTGATCAGCGGGTGA

Upstream 100 bases:

>100_bases
CGTCATCCCTGATGTTCGACTTCGGGCCTCTCTTTTCATCCGGCGATTTTGCTCTAAAAACAATGCAGATTTCTGGCCAG
ATTCTCTGGAGTGATATTCC

Downstream 100 bases:

>100_bases
GGGGCTGACGGCGAGCCGCCAGCCTCGTTCGCTCATAGCCTGAGCCGGGCCATCGTATAGGCGTCGACATACTGTCCCGA
TCGATAACCGAAGGCCCTCA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MSTEGSAVDTLTIEEAAAELERLAKEIAHHDALYHGKDRPQISDADYDALKRRNDALEVQFPELIREDSPSRRVGAAPSV
TFSPVVHARPMLSLDNTFSQEDVQDFVASVYRFLGRLPDQSIAFTAEPKIDGLSMSIRYENGRLVTAATRGDGTTGENVT
ANIRTIAEIPNELPKGVPAVVEIRGEVYMAKSDFLALNRQMEAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLKFFAYAW
GEMSEMPADTQFDMVQTFKDWGFPVNPLMKRLNSVADILAHYDEIGLERPDLDYDIDGVVYKVDSLELQARLGFRSRSPR
WATAHKFPAEQAFTEVEKIEIQVGRTGALTPVARLKPITVGGVVVTNATLHNEDYIKGIGNSGERIRPEDHDIREGDTVI
VQRAGDVIPQILDVVMEKRTADARSYEFPKTCPVCGSHAVREVNEKTGKMDSVRRCTGGFICRAQATEHLKHFVSRNAFD
IEGLGSKQIDFFFEHEDASLQIRTAPEIFTLEKRQQASLTKLENIDGFGKVSVGKLYTAINERRSIALHRFIYALGIRHV
GETTAKLLARSYGTYEAFAMAMKEAAPLSGDAWNDLNAIEGIGEVVARAMVEFYKEPRNVEVITRLIEEVTPQQAEQPVT
TGSPVAGKTVVFTGSLEKFTRDEAKARAESLGAKVAGSVSKKTDIVVAGPGAGSKLDKARELGVQTMDEDEWLALISG

Sequences:

>Translated_718_residues
MSTEGSAVDTLTIEEAAAELERLAKEIAHHDALYHGKDRPQISDADYDALKRRNDALEVQFPELIREDSPSRRVGAAPSV
TFSPVVHARPMLSLDNTFSQEDVQDFVASVYRFLGRLPDQSIAFTAEPKIDGLSMSIRYENGRLVTAATRGDGTTGENVT
ANIRTIAEIPNELPKGVPAVVEIRGEVYMAKSDFLALNRQMEAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLKFFAYAW
GEMSEMPADTQFDMVQTFKDWGFPVNPLMKRLNSVADILAHYDEIGLERPDLDYDIDGVVYKVDSLELQARLGFRSRSPR
WATAHKFPAEQAFTEVEKIEIQVGRTGALTPVARLKPITVGGVVVTNATLHNEDYIKGIGNSGERIRPEDHDIREGDTVI
VQRAGDVIPQILDVVMEKRTADARSYEFPKTCPVCGSHAVREVNEKTGKMDSVRRCTGGFICRAQATEHLKHFVSRNAFD
IEGLGSKQIDFFFEHEDASLQIRTAPEIFTLEKRQQASLTKLENIDGFGKVSVGKLYTAINERRSIALHRFIYALGIRHV
GETTAKLLARSYGTYEAFAMAMKEAAPLSGDAWNDLNAIEGIGEVVARAMVEFYKEPRNVEVITRLIEEVTPQQAEQPVT
TGSPVAGKTVVFTGSLEKFTRDEAKARAESLGAKVAGSVSKKTDIVVAGPGAGSKLDKARELGVQTMDEDEWLALISG
>Mature_717_residues
STEGSAVDTLTIEEAAAELERLAKEIAHHDALYHGKDRPQISDADYDALKRRNDALEVQFPELIREDSPSRRVGAAPSVT
FSPVVHARPMLSLDNTFSQEDVQDFVASVYRFLGRLPDQSIAFTAEPKIDGLSMSIRYENGRLVTAATRGDGTTGENVTA
NIRTIAEIPNELPKGVPAVVEIRGEVYMAKSDFLALNRQMEAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLKFFAYAWG
EMSEMPADTQFDMVQTFKDWGFPVNPLMKRLNSVADILAHYDEIGLERPDLDYDIDGVVYKVDSLELQARLGFRSRSPRW
ATAHKFPAEQAFTEVEKIEIQVGRTGALTPVARLKPITVGGVVVTNATLHNEDYIKGIGNSGERIRPEDHDIREGDTVIV
QRAGDVIPQILDVVMEKRTADARSYEFPKTCPVCGSHAVREVNEKTGKMDSVRRCTGGFICRAQATEHLKHFVSRNAFDI
EGLGSKQIDFFFEHEDASLQIRTAPEIFTLEKRQQASLTKLENIDGFGKVSVGKLYTAINERRSIALHRFIYALGIRHVG
ETTAKLLARSYGTYEAFAMAMKEAAPLSGDAWNDLNAIEGIGEVVARAMVEFYKEPRNVEVITRLIEEVTPQQAEQPVTT
GSPVAGKTVVFTGSLEKFTRDEAKARAESLGAKVAGSVSKKTDIVVAGPGAGSKLDKARELGVQTMDEDEWLALISG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=713, Percent_Identity=46.5638148667602, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI87082305, Length=548, Percent_Identity=22.992700729927, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 79215; Mature: 79084

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50172 BRCT ; PS00913 ADH_IRON_1 ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEGSAVDTLTIEEAAAELERLAKEIAHHDALYHGKDRPQISDADYDALKRRNDALEVQ
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEE
FPELIREDSPSRRVGAAPSVTFSPVVHARPMLSLDNTFSQEDVQDFVASVYRFLGRLPDQ
CHHHHCCCCCCCCCCCCCCCEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
SIAFTAEPKIDGLSMSIRYENGRLVTAATRGDGTTGENVTANIRTIAEIPNELPKGVPAV
CEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHCCCCCEE
VEIRGEVYMAKSDFLALNRQMEAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLKFFAYAW
EEECCCEEEECCCHHHHCCHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
GEMSEMPADTQFDMVQTFKDWGFPVNPLMKRLNSVADILAHYDEIGLERPDLDYDIDGVV
CHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
YKVDSLELQARLGFRSRSPRWATAHKFPAEQAFTEVEKIEIQVGRTGALTPVARLKPITV
EEECCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCHHHHCCCEEE
GGVVVTNATLHNEDYIKGIGNSGERIRPEDHDIREGDTVIVQRAGDVIPQILDVVMEKRT
CCEEEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHC
ADARSYEFPKTCPVCGSHAVREVNEKTGKMDSVRRCTGGFICRAQATEHLKHFVSRNAFD
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCC
IEGLGSKQIDFFFEHEDASLQIRTAPEIFTLEKRQQASLTKLENIDGFGKVSVGKLYTAI
CCCCCCCCEEEEEECCCCEEEEEECCCEEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHH
NERRSIALHRFIYALGIRHVGETTAKLLARSYGTYEAFAMAMKEAAPLSGDAWNDLNAIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
GIGEVVARAMVEFYKEPRNVEVITRLIEEVTPQQAEQPVTTGSPVAGKTVVFTGSLEKFT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHCCCCCCCCCCCCCEEEEECCHHHHH
RDEAKARAESLGAKVAGSVSKKTDIVVAGPGAGSKLDKARELGVQTMDEDEWLALISG
HHHHHHHHHHHCHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
STEGSAVDTLTIEEAAAELERLAKEIAHHDALYHGKDRPQISDADYDALKRRNDALEVQ
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEE
FPELIREDSPSRRVGAAPSVTFSPVVHARPMLSLDNTFSQEDVQDFVASVYRFLGRLPDQ
CHHHHCCCCCCCCCCCCCCCEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
SIAFTAEPKIDGLSMSIRYENGRLVTAATRGDGTTGENVTANIRTIAEIPNELPKGVPAV
CEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHCCCCCEE
VEIRGEVYMAKSDFLALNRQMEAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLKFFAYAW
EEECCCEEEECCCHHHHCCHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
GEMSEMPADTQFDMVQTFKDWGFPVNPLMKRLNSVADILAHYDEIGLERPDLDYDIDGVV
CHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
YKVDSLELQARLGFRSRSPRWATAHKFPAEQAFTEVEKIEIQVGRTGALTPVARLKPITV
EEECCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCHHHHCCCEEE
GGVVVTNATLHNEDYIKGIGNSGERIRPEDHDIREGDTVIVQRAGDVIPQILDVVMEKRT
CCEEEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHC
ADARSYEFPKTCPVCGSHAVREVNEKTGKMDSVRRCTGGFICRAQATEHLKHFVSRNAFD
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCC
IEGLGSKQIDFFFEHEDASLQIRTAPEIFTLEKRQQASLTKLENIDGFGKVSVGKLYTAI
CCCCCCCCEEEEEECCCCEEEEEECCCEEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHH
NERRSIALHRFIYALGIRHVGETTAKLLARSYGTYEAFAMAMKEAAPLSGDAWNDLNAIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
GIGEVVARAMVEFYKEPRNVEVITRLIEEVTPQQAEQPVTTGSPVAGKTVVFTGSLEKFT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHCCCCCCCCCCCCCEEEEECCHHHHH
RDEAKARAESLGAKVAGSVSKKTDIVVAGPGAGSKLDKARELGVQTMDEDEWLALISG
HHHHHHHHHHHCHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA