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The map label for this gene is glcD [H]

Identifier: 209550130

GI number: 209550130

Start: 2581164

End: 2582576

Strand: Reverse

Name: glcD [H]

Synonym: Rleg2_2548

Alternate gene names: 209550130

Gene position: 2582576-2581164 (Counterclockwise)

Preceding gene: 209550131

Following gene: 209550129

Centisome position: 56.91

GC content: 63.2

Gene sequence:

>1413_bases
ATGGCGCTCAAGGACGCGAAGGCCGGCACACGCAACGAGGCGGGGATATCAGCCGTGCTCGGCATTCTCAAGCAGAGCTT
CGGCGAGCGCTTCCAGACCGGCCAGTCCTTCCGCGAACAGCATGCCCACACGACCACCTATATTCCGCCGCAGCTGCCTG
ATGGCGTGCTCTTTGCCGAGAGCGCCGACGATGTAAAGGCGGTGGTCAGGGCGTGTGCGATCCACAAGGTGCCGGTGATC
GGTTTCGGTGTCGGGACCTCGCTGGAGGGCCAGGTCAATGCCGCCAATGGCGGTATTTCGATCGATTTCAGCCGCATGAA
CCGGGTGCTCGAGGTCAATCCCGAGGATCTCGACTGCACCGTCGAGCCGGGTGTCACGCGCGAGGCGCTGAACATCCATC
TGCGCGATACCGGACTGTTCTTCCCGATCGATCCCGGCGCCAATGCCTCGATCGGCGGCATGGCCTCGACGCGGGCCTCC
GGCACCAATGCCGTGCGCTATGGGACGATGAAGGACAATGTCCTTGCCGTGACCGCCGTTACCGCCAATGGCGAGGAGAT
CCGCACGGCCCGGCGCGCCCGCAAATCTTCTGCCGGCTACGACCTGACGCGGCTCTTCGTCGGCGCCGAGGGCACGCTCG
GCATCCTGACCTCGGTGACGCTGCGCCTGCAGGCGATCCCGCAGAAGATCGCCGGCGGCGCCTGCGCTTTTCCGAGCATC
AAGGCCGCTTGCGATGCCGTCATCATGACGATCCAGATGGGTATTCCGGTGGCGCGTATCGAGCTGCTCGATACGGTGCA
GATTCGGGCCTGCAATGCCTATTCCAAGCTTTCCTATGCCGAAAGCCCAACGCTCTTCCTCGAATTCCACGGCACTGACG
AGACCGTGCCGCTGCAATCGGCAGGCTTTGGCGAGATTGCGGCGGAATGCGGCGGGGGCGAGTTCTTGTGGACCGCCAAT
GCCGAGGAGCGGACCAAGCTCTGGAAGGCGCGCCACGATGCCTATTGGTCCGCAAGGGCGCTGGCGCCGGGGCTTGCCGC
ACTTTCGACGGATGTTTGTGTTCCGATATCGCGGCTTGCCGATTGTGTTTCCGAAACGCAGGCCGATATCGAGACCCATG
GAATGCTCGGCCCGATCGTCGGCCATGCCGGCGACGGGAACTTCCATGTGCTGCTGTTGTTCGATGACAAAAGCGCCGAA
GACATCGCCAAGGCCGAGGGCTTCGTGCAGCGGCTCAATCGGCGGGCGCTCGACATGGACGGGACATGCACCGGCGAACA
CGGGATCGGGCAAGGCAAGATGGCGTTCCTCGAACAAGAGCTCGGGGGCACGGTGGATCTGATGCGACAGGTGAAACAGG
CGCTCGATCCGGACAATATCTTCAATCCCGGCAAGATTTTCCACCAGGGCTGA

Upstream 100 bases:

>100_bases
TCTCCCAATCTTAACTGGACAGTCGCTTTGACTTGCCGCATACCCAGCCTGACCCCATGGGGTACGACATGTCCCATGGA
ATGGCAGGGAGATCAAAGGG

Downstream 100 bases:

>100_bases
AAACGGAATATTCTCATGAACTTGGGCCTTGCCCCCGGCATGAATAGCGCTAGTGTCGGCAACAGAATGCTGAATGGAGA
ATCCTTGCATTGGTAGGCCG

Product: D-lactate dehydrogenase

Products: (R)-acetoin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MALKDAKAGTRNEAGISAVLGILKQSFGERFQTGQSFREQHAHTTTYIPPQLPDGVLFAESADDVKAVVRACAIHKVPVI
GFGVGTSLEGQVNAANGGISIDFSRMNRVLEVNPEDLDCTVEPGVTREALNIHLRDTGLFFPIDPGANASIGGMASTRAS
GTNAVRYGTMKDNVLAVTAVTANGEEIRTARRARKSSAGYDLTRLFVGAEGTLGILTSVTLRLQAIPQKIAGGACAFPSI
KAACDAVIMTIQMGIPVARIELLDTVQIRACNAYSKLSYAESPTLFLEFHGTDETVPLQSAGFGEIAAECGGGEFLWTAN
AEERTKLWKARHDAYWSARALAPGLAALSTDVCVPISRLADCVSETQADIETHGMLGPIVGHAGDGNFHVLLLFDDKSAE
DIAKAEGFVQRLNRRALDMDGTCTGEHGIGQGKMAFLEQELGGTVDLMRQVKQALDPDNIFNPGKIFHQG

Sequences:

>Translated_470_residues
MALKDAKAGTRNEAGISAVLGILKQSFGERFQTGQSFREQHAHTTTYIPPQLPDGVLFAESADDVKAVVRACAIHKVPVI
GFGVGTSLEGQVNAANGGISIDFSRMNRVLEVNPEDLDCTVEPGVTREALNIHLRDTGLFFPIDPGANASIGGMASTRAS
GTNAVRYGTMKDNVLAVTAVTANGEEIRTARRARKSSAGYDLTRLFVGAEGTLGILTSVTLRLQAIPQKIAGGACAFPSI
KAACDAVIMTIQMGIPVARIELLDTVQIRACNAYSKLSYAESPTLFLEFHGTDETVPLQSAGFGEIAAECGGGEFLWTAN
AEERTKLWKARHDAYWSARALAPGLAALSTDVCVPISRLADCVSETQADIETHGMLGPIVGHAGDGNFHVLLLFDDKSAE
DIAKAEGFVQRLNRRALDMDGTCTGEHGIGQGKMAFLEQELGGTVDLMRQVKQALDPDNIFNPGKIFHQG
>Mature_469_residues
ALKDAKAGTRNEAGISAVLGILKQSFGERFQTGQSFREQHAHTTTYIPPQLPDGVLFAESADDVKAVVRACAIHKVPVIG
FGVGTSLEGQVNAANGGISIDFSRMNRVLEVNPEDLDCTVEPGVTREALNIHLRDTGLFFPIDPGANASIGGMASTRASG
TNAVRYGTMKDNVLAVTAVTANGEEIRTARRARKSSAGYDLTRLFVGAEGTLGILTSVTLRLQAIPQKIAGGACAFPSIK
AACDAVIMTIQMGIPVARIELLDTVQIRACNAYSKLSYAESPTLFLEFHGTDETVPLQSAGFGEIAAECGGGEFLWTANA
EERTKLWKARHDAYWSARALAPGLAALSTDVCVPISRLADCVSETQADIETHGMLGPIVGHAGDGNFHVLLLFDDKSAED
IAKAEGFVQRLNRRALDMDGTCTGEHGIGQGKMAFLEQELGGTVDLMRQVKQALDPDNIFNPGKIFHQG

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=444, Percent_Identity=49.7747747747748, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI37595754, Length=467, Percent_Identity=47.3233404710921, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI119964728, Length=421, Percent_Identity=27.790973871734, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI4501993, Length=454, Percent_Identity=24.8898678414097, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1789351, Length=439, Percent_Identity=28.4738041002278, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1787977, Length=499, Percent_Identity=27.4549098196393, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI48994907, Length=470, Percent_Identity=21.9148936170213, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI48994914, Length=134, Percent_Identity=29.1044776119403, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71992373, Length=441, Percent_Identity=47.6190476190476, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71992381, Length=441, Percent_Identity=46.2585034013605, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71992392, Length=376, Percent_Identity=50.7978723404255, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17534361, Length=427, Percent_Identity=25.5269320843091, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17556096, Length=226, Percent_Identity=27.4336283185841, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320027, Length=477, Percent_Identity=37.9454926624738, Blast_Score=308, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6320764, Length=454, Percent_Identity=27.7533039647577, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320023, Length=439, Percent_Identity=25.5125284738041, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI18921117, Length=435, Percent_Identity=28.735632183908, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24639277, Length=435, Percent_Identity=28.735632183908, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24639275, Length=435, Percent_Identity=28.735632183908, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24653753, Length=458, Percent_Identity=26.2008733624454, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.1.4

Molecular weight: Translated: 50015; Mature: 49884

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKDAKAGTRNEAGISAVLGILKQSFGERFQTGQSFREQHAHTTTYIPPQLPDGVLFAE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEC
SADDVKAVVRACAIHKVPVIGFGVGTSLEGQVNAANGGISIDFSRMNRVLEVNPEDLDCT
CCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCEEEEHHHCCCEEECCCCCCCEE
VEPGVTREALNIHLRDTGLFFPIDPGANASIGGMASTRASGTNAVRYGTMKDNVLAVTAV
ECCCCCEEEEEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEE
TANGEEIRTARRARKSSAGYDLTRLFVGAEGTLGILTSVTLRLQAIPQKIAGGACAFPSI
ECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCH
KAACDAVIMTIQMGIPVARIELLDTVQIRACNAYSKLSYAESPTLFLEFHGTDETVPLQS
HHHHHHHEEEHHCCCCEEEEEHHHHEEEEECCHHHHCCCCCCCEEEEEECCCCCCCCCCC
AGFGEIAAECGGGEFLWTANAEERTKLWKARHDAYWSARALAPGLAALSTDVCVPISRLA
CCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHH
DCVSETQADIETHGMLGPIVGHAGDGNFHVLLLFDDKSAEDIAKAEGFVQRLNRRALDMD
HHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
GTCTGEHGIGQGKMAFLEQELGGTVDLMRQVKQALDPDNIFNPGKIFHQG
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALKDAKAGTRNEAGISAVLGILKQSFGERFQTGQSFREQHAHTTTYIPPQLPDGVLFAE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEC
SADDVKAVVRACAIHKVPVIGFGVGTSLEGQVNAANGGISIDFSRMNRVLEVNPEDLDCT
CCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCEEEEHHHCCCEEECCCCCCCEE
VEPGVTREALNIHLRDTGLFFPIDPGANASIGGMASTRASGTNAVRYGTMKDNVLAVTAV
ECCCCCEEEEEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEE
TANGEEIRTARRARKSSAGYDLTRLFVGAEGTLGILTSVTLRLQAIPQKIAGGACAFPSI
ECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCH
KAACDAVIMTIQMGIPVARIELLDTVQIRACNAYSKLSYAESPTLFLEFHGTDETVPLQS
HHHHHHHEEEHHCCCCEEEEEHHHHEEEEECCHHHHCCCCCCCEEEEEECCCCCCCCCCC
AGFGEIAAECGGGEFLWTANAEERTKLWKARHDAYWSARALAPGLAALSTDVCVPISRLA
CCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHH
DCVSETQADIETHGMLGPIVGHAGDGNFHVLLLFDDKSAEDIAKAEGFVQRLNRRALDMD
HHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
GTCTGEHGIGQGKMAFLEQELGGTVDLMRQVKQALDPDNIFNPGKIFHQG
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R,R)-butane-2,3-diol; NAD+

Specific reaction: (R,R)-butane-2,3-diol + NAD+ = (R)-acetoin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]