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The map label for this gene is hphIAM [H]

Identifier: 209550032

GI number: 209550032

Start: 2494063

End: 2496003

Strand: Reverse

Name: hphIAM [H]

Synonym: Rleg2_2449

Alternate gene names: 209550032

Gene position: 2496003-2494063 (Counterclockwise)

Preceding gene: 209550033

Following gene: 209550031

Centisome position: 55.0

GC content: 60.95

Gene sequence:

>1941_bases
GTGGCGCGCAAGATCAAAGTCGCAGATTTGCTTTGCGGGGCCGGTGGTTCGTCAGCAGGGGCGAAGCGCGCGCTGGAAGA
AATGGGCCTGGAGATGGAATTGGTCTGTGTAAATCACTGGCCGACTGCCATCGATACCCACCAGAGGAATTTTCCCGAAG
CCAGACACTACATTCAGGACATCGCCACAGTCCGCCCGCACATCCTGGTGCCGGAAGGCTATCTGGATCTCTTGATGGCG
TCCCCGACGTGCACGCATCATTCTGTGGCGCGCGGCGGCAAGCCGACCAGCGACCAGCAGCGCAGCGACCCTTGGCATAT
CGTCACCTGGCTGACCGAGTTGCGGGTAAAGCGGATCATCATCGAAAACGTCTGGGAGTTCTGCGGCTGGGGCCCGGTCA
ACATGAAGACCGGTCGCCCTATCCCGTCCAGAAAGGGCGAATATTTCCACGCATGGACTGAGACGCTCCGGCGTCTTGGC
TTCGAACTGGAGTGGCGCCGACTCAACGCCGCCGACTACGGGGACGCGACCACGCGGCAACGCTTCATCCTCATGGGTCG
ATCCGACGGCCGCAAGATCCACTGGCCAATGCCGACGCACCGAAAGCGCGATGAGGTCAACGCAGATCTGTTCTCCGCCG
CAGAGCCGTGGCGCCCGGCGCGGGAGATCATCGACTGGTCTATCAAGGGGCGCTCCATCCTCAACCGCAAGAAGCCGTTG
GCGCCCAAGACGCTAGCGCGGGTGTTGGCCGGCGCATTTAAGTTCGGCTGGCCGAAACCGTTCATCGACAAGCTTATGGA
AGAGATCGAGCGGTCCCTGCGCTACCATATCAATTGGGCCTTCGAAGCACGAAACGCAAAGGCCTCAAAGTCCAAACGTC
GTCAGCGTCGTGCACTGGCGAAGGATTTGATCAGGCGCCTACGCCACTTCAGGATTGCGCCTGCCGAGTACGCCAAGGGA
GGGCGGGCCGCTGAGCCTATGGTCATCACGTTGCGGCGCAACGGTAACGGCACGTCGATATCAAGCCCCATCCCCACGGT
CGCTGCAAATGGGCAGCATGTTGGACTTGCCGAGCCCGTCATCGTCAACATGAAGGGCCAATCGACGGCGACTTCGTCGA
GAGAGCCCTTACCCACGCAAACGTCGCATGCGGCTCATTTATACGCTGCAGAGCCAATCATTCTCTCACAGCACAATAGC
GGGTCAGCTCGAGAGGTGAGTGATCCTCTCCCCACTATCACGACCGGAGGAGCAGCGAACGAAGCCCGGCCAGGTTGCGC
TAGGCCTATGCTTGTCGAGCCGTTCGTTCTGTCTCAAGCATCTGGAGGCTCCCCGCGGGCCGTTAGCGACCCCATCCCGA
CACCGACGACAGGCGGCAACGGGGCGGCGCACGCGCTCATCTCTCCATACTATGGCTCCGGTTCCGGCGAGACCTGCAAT
CATGTCGACGAGGTGTTGCCGACCATAACCAGCAAAGGCCGTTTCGGCATGGTGGTGCCGGTCACCAATTCAAACGGTGG
AGCGACGGCGCGAAACATCGATGTTGACCCGGTTCCCACCATGACCACCGCGAAGGGCGGTGAGTTCGCGTTCATCGCCG
CACAGTTCGGCGAGCGGGAAGGCCAGGCGCCGCGCGTCCACGATATCGACCAACCCACGCCGACGATCGCGGCAACCGGC
CATATCAACCTCGTCGAGTCTGGGCCGGAATACGACATCCTCTTCCGCATGCTGGAGCCGCATGAGCTGGCGGCGGCAAT
GGGCTTCAATACCGAAGAGGCGACGTATGAGTTCGCCGGCACCAAGACCGAAAAAATCAAGCAGATTGGCAACGCCGTCT
CGGTGGCGAAGATGAAGGCTTGCGTCGGTGCAATCATGGCAGACGCGGTGCCGAAGCTGAAATCGAGGCCAGATGCTGAA
TTTCTGGAGGCCGCTGAATGA

Upstream 100 bases:

>100_bases
AAAGGTTGCCGCTTTCAGCGAGAGCGTGGCTGGCCTCTCGGTGGCGGGCGCGGGCGAATAAATGGGCCGATCGGGCCGAA
TGGATCATGGGGGATTGAGA

Downstream 100 bases:

>100_bases
CCGAACCGTCAGAAATGATCGCATGGCTCGACCGCCGCATCGCCTCCGCCATGACCTGGCTGGATGATCACGGCAAAGGC
TCGAAGAAGCCCCGCCCAGA

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.HphIA; Cytosine-specific methyltransferase HphIA; M.Hphi(C) [H]

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MARKIKVADLLCGAGGSSAGAKRALEEMGLEMELVCVNHWPTAIDTHQRNFPEARHYIQDIATVRPHILVPEGYLDLLMA
SPTCTHHSVARGGKPTSDQQRSDPWHIVTWLTELRVKRIIIENVWEFCGWGPVNMKTGRPIPSRKGEYFHAWTETLRRLG
FELEWRRLNAADYGDATTRQRFILMGRSDGRKIHWPMPTHRKRDEVNADLFSAAEPWRPAREIIDWSIKGRSILNRKKPL
APKTLARVLAGAFKFGWPKPFIDKLMEEIERSLRYHINWAFEARNAKASKSKRRQRRALAKDLIRRLRHFRIAPAEYAKG
GRAAEPMVITLRRNGNGTSISSPIPTVAANGQHVGLAEPVIVNMKGQSTATSSREPLPTQTSHAAHLYAAEPIILSQHNS
GSAREVSDPLPTITTGGAANEARPGCARPMLVEPFVLSQASGGSPRAVSDPIPTPTTGGNGAAHALISPYYGSGSGETCN
HVDEVLPTITSKGRFGMVVPVTNSNGGATARNIDVDPVPTMTTAKGGEFAFIAAQFGEREGQAPRVHDIDQPTPTIAATG
HINLVESGPEYDILFRMLEPHELAAAMGFNTEEATYEFAGTKTEKIKQIGNAVSVAKMKACVGAIMADAVPKLKSRPDAE
FLEAAE

Sequences:

>Translated_646_residues
MARKIKVADLLCGAGGSSAGAKRALEEMGLEMELVCVNHWPTAIDTHQRNFPEARHYIQDIATVRPHILVPEGYLDLLMA
SPTCTHHSVARGGKPTSDQQRSDPWHIVTWLTELRVKRIIIENVWEFCGWGPVNMKTGRPIPSRKGEYFHAWTETLRRLG
FELEWRRLNAADYGDATTRQRFILMGRSDGRKIHWPMPTHRKRDEVNADLFSAAEPWRPAREIIDWSIKGRSILNRKKPL
APKTLARVLAGAFKFGWPKPFIDKLMEEIERSLRYHINWAFEARNAKASKSKRRQRRALAKDLIRRLRHFRIAPAEYAKG
GRAAEPMVITLRRNGNGTSISSPIPTVAANGQHVGLAEPVIVNMKGQSTATSSREPLPTQTSHAAHLYAAEPIILSQHNS
GSAREVSDPLPTITTGGAANEARPGCARPMLVEPFVLSQASGGSPRAVSDPIPTPTTGGNGAAHALISPYYGSGSGETCN
HVDEVLPTITSKGRFGMVVPVTNSNGGATARNIDVDPVPTMTTAKGGEFAFIAAQFGEREGQAPRVHDIDQPTPTIAATG
HINLVESGPEYDILFRMLEPHELAAAMGFNTEEATYEFAGTKTEKIKQIGNAVSVAKMKACVGAIMADAVPKLKSRPDAE
FLEAAE
>Mature_645_residues
ARKIKVADLLCGAGGSSAGAKRALEEMGLEMELVCVNHWPTAIDTHQRNFPEARHYIQDIATVRPHILVPEGYLDLLMAS
PTCTHHSVARGGKPTSDQQRSDPWHIVTWLTELRVKRIIIENVWEFCGWGPVNMKTGRPIPSRKGEYFHAWTETLRRLGF
ELEWRRLNAADYGDATTRQRFILMGRSDGRKIHWPMPTHRKRDEVNADLFSAAEPWRPAREIIDWSIKGRSILNRKKPLA
PKTLARVLAGAFKFGWPKPFIDKLMEEIERSLRYHINWAFEARNAKASKSKRRQRRALAKDLIRRLRHFRIAPAEYAKGG
RAAEPMVITLRRNGNGTSISSPIPTVAANGQHVGLAEPVIVNMKGQSTATSSREPLPTQTSHAAHLYAAEPIILSQHNSG
SAREVSDPLPTITTGGAANEARPGCARPMLVEPFVLSQASGGSPRAVSDPIPTPTTGGNGAAHALISPYYGSGSGETCNH
VDEVLPTITSKGRFGMVVPVTNSNGGATARNIDVDPVPTMTTAKGGEFAFIAAQFGEREGQAPRVHDIDQPTPTIAATGH
INLVESGPEYDILFRMLEPHELAAAMGFNTEEATYEFAGTKTEKIKQIGNAVSVAKMKACVGAIMADAVPKLKSRPDAEF
LEAAE

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 70834; Mature: 70702

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKIKVADLLCGAGGSSAGAKRALEEMGLEMELVCVNHWPTAIDTHQRNFPEARHYIQD
CCCEEEHHHHEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHH
IATVRPHILVPEGYLDLLMASPTCTHHSVARGGKPTSDQQRSDPWHIVTWLTELRVKRII
HHHCCCEEECCCCHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IENVWEFCGWGPVNMKTGRPIPSRKGEYFHAWTETLRRLGFELEWRRLNAADYGDATTRQ
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCCE
RFILMGRSDGRKIHWPMPTHRKRDEVNADLFSAAEPWRPAREIIDWSIKGRSILNRKKPL
EEEEEECCCCCEEECCCCCCCCCHHCCHHHHHCCCCCCHHHHHHCCCCCCHHHHHCCCCC
APKTLARVLAGAFKFGWPKPFIDKLMEEIERSLRYHINWAFEARNAKASKSKRRQRRALA
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHH
KDLIRRLRHFRIAPAEYAKGGRAAEPMVITLRRNGNGTSISSPIPTVAANGQHVGLAEPV
HHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCE
IVNMKGQSTATSSREPLPTQTSHAAHLYAAEPIILSQHNSGSAREVSDPLPTITTGGAAN
EEEECCCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCCEECCCCCC
EARPGCARPMLVEPFVLSQASGGSPRAVSDPIPTPTTGGNGAAHALISPYYGSGSGETCN
CCCCCCCCCCEECCHHEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHH
HVDEVLPTITSKGRFGMVVPVTNSNGGATARNIDVDPVPTMTTAKGGEFAFIAAQFGERE
HHHHHHHHHCCCCCEEEEEEEECCCCCCEEECCCCCCCCCEEECCCCCEEEEEEECCCCC
GQAPRVHDIDQPTPTIAATGHINLVESGPEYDILFRMLEPHELAAAMGFNTEEATYEFAG
CCCCCCCCCCCCCCCEEECCEEEEECCCCCHHHHHHHCCHHHHHHHHCCCCCCCEEEECC
TKTEKIKQIGNAVSVAKMKACVGAIMADAVPKLKSRPDAEFLEAAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure 
ARKIKVADLLCGAGGSSAGAKRALEEMGLEMELVCVNHWPTAIDTHQRNFPEARHYIQD
CCEEEHHHHEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHH
IATVRPHILVPEGYLDLLMASPTCTHHSVARGGKPTSDQQRSDPWHIVTWLTELRVKRII
HHHCCCEEECCCCHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IENVWEFCGWGPVNMKTGRPIPSRKGEYFHAWTETLRRLGFELEWRRLNAADYGDATTRQ
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCCE
RFILMGRSDGRKIHWPMPTHRKRDEVNADLFSAAEPWRPAREIIDWSIKGRSILNRKKPL
EEEEEECCCCCEEECCCCCCCCCHHCCHHHHHCCCCCCHHHHHHCCCCCCHHHHHCCCCC
APKTLARVLAGAFKFGWPKPFIDKLMEEIERSLRYHINWAFEARNAKASKSKRRQRRALA
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHH
KDLIRRLRHFRIAPAEYAKGGRAAEPMVITLRRNGNGTSISSPIPTVAANGQHVGLAEPV
HHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCE
IVNMKGQSTATSSREPLPTQTSHAAHLYAAEPIILSQHNSGSAREVSDPLPTITTGGAAN
EEEECCCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCCEECCCCCC
EARPGCARPMLVEPFVLSQASGGSPRAVSDPIPTPTTGGNGAAHALISPYYGSGSGETCN
CCCCCCCCCCEECCHHEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHH
HVDEVLPTITSKGRFGMVVPVTNSNGGATARNIDVDPVPTMTTAKGGEFAFIAAQFGERE
HHHHHHHHHCCCCCEEEEEEEECCCCCCEEECCCCCCCCCEEECCCCCEEEEEEECCCCC
GQAPRVHDIDQPTPTIAATGHINLVESGPEYDILFRMLEPHELAAAMGFNTEEATYEFAG
CCCCCCCCCCCCCCCEEECCEEEEECCCCCHHHHHHHCCHHHHHHHHCCCCCCCEEEECC
TKTEKIKQIGNAVSVAKMKACVGAIMADAVPKLKSRPDAEFLEAAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]