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The map label for this gene is yafC [H]

Identifier: 209549950

GI number: 209549950

Start: 2426014

End: 2426916

Strand: Reverse

Name: yafC [H]

Synonym: Rleg2_2366

Alternate gene names: 209549950

Gene position: 2426916-2426014 (Counterclockwise)

Preceding gene: 209549953

Following gene: 209549949

Centisome position: 53.48

GC content: 62.13

Gene sequence:

>903_bases
ATGGCGGACCTGAACGACATCGCGGTTTTCGTGACGGTGGCGCGGTACGGCAGCTTCAGCCGCGCCGCCCATTCTCTCGG
CATGCCGGTCTCGACCGTCAGCCGGAAGGTGACGTCGCTGGAGGAGCAACTCGGCGTGACGCTGCTGCAGCGCACGACGC
GTAAGCTCAGCCTGACCGCGCAGGGCAGCGCCTATTTCGATCAGTGCAGCGAGCCGCTCGCCCATCTTATCGACGCCGAG
CAGGCACTGACGGAAACCCAGAGAAAACCGGAGGGCTTGCTGAAGATCTCCGTGCCCGTCATCTTCGGGCAGGAGGTTTT
CTACGAATTCGTTTCGGCCTTCCTGAAGACCTATCCCGAAATCCGGATCGATCTCTTCGTCACCAACCTGTTCCTCGATC
TGATCGCCGAGAATATCGATCTCGGGATCCGTTTCGGCGAGCTCAAGGATTCGTCGATCGTCGCGCAGCGGCTCGGCAAG
AGCGTACGCTATCTGGTGGCCGCGCCGGACTATATGAAGGGCAGGGCGCTTCCCTCGAAGCCCGAAGACCTGAAGGACCA
TCAGTGCGTGCTGCTGAACGGCCGCAACGGCGAAGCGGAATGGCATCTCGTCAGCGGCCGCAAATCGGTCCGCCAGCAGG
TGTCGGGGCCGGTCTCCAGCCGGGATTTCGAGGCGGTGAGCGCCTTCACCTATCGCGGACACGGCATCGGCCTGCTGCCT
TCCACCTATTGCGACGATGAGATCAGAAGAGGTGAACTCGTCCGCCTGTTGCCGGACTGGTCGTCCGAGGAGATATTCGT
GCATGCGGTCTATCCGACACGCCGCTTCCTTCCCTTGCGCCTGCAGGTCTTTCTCGAGGCGCTGAAGGCGTGGAAGACCC
CCTTGTGGCTCCCCCTGCATTGA

Upstream 100 bases:

>100_bases
GGGTGTGATTGTTTTCGCAAGGTGAATATGGCGAAAACTCCATTCCTCGATTAGATGGAAATATCTGGATTCTCTTTTGC
ATCTATGCAAAAATCGCTGC

Downstream 100 bases:

>100_bases
GTCCCCCGATGCCATCACCGAAAATTGAAGCCGCGCCCGCGGGCCGGCGTCCCTGGTCGATCAAGCTGTGTTACAGTTCG
CTGGCTTGGATGGCGCGGGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MADLNDIAVFVTVARYGSFSRAAHSLGMPVSTVSRKVTSLEEQLGVTLLQRTTRKLSLTAQGSAYFDQCSEPLAHLIDAE
QALTETQRKPEGLLKISVPVIFGQEVFYEFVSAFLKTYPEIRIDLFVTNLFLDLIAENIDLGIRFGELKDSSIVAQRLGK
SVRYLVAAPDYMKGRALPSKPEDLKDHQCVLLNGRNGEAEWHLVSGRKSVRQQVSGPVSSRDFEAVSAFTYRGHGIGLLP
STYCDDEIRRGELVRLLPDWSSEEIFVHAVYPTRRFLPLRLQVFLEALKAWKTPLWLPLH

Sequences:

>Translated_300_residues
MADLNDIAVFVTVARYGSFSRAAHSLGMPVSTVSRKVTSLEEQLGVTLLQRTTRKLSLTAQGSAYFDQCSEPLAHLIDAE
QALTETQRKPEGLLKISVPVIFGQEVFYEFVSAFLKTYPEIRIDLFVTNLFLDLIAENIDLGIRFGELKDSSIVAQRLGK
SVRYLVAAPDYMKGRALPSKPEDLKDHQCVLLNGRNGEAEWHLVSGRKSVRQQVSGPVSSRDFEAVSAFTYRGHGIGLLP
STYCDDEIRRGELVRLLPDWSSEEIFVHAVYPTRRFLPLRLQVFLEALKAWKTPLWLPLH
>Mature_299_residues
ADLNDIAVFVTVARYGSFSRAAHSLGMPVSTVSRKVTSLEEQLGVTLLQRTTRKLSLTAQGSAYFDQCSEPLAHLIDAEQ
ALTETQRKPEGLLKISVPVIFGQEVFYEFVSAFLKTYPEIRIDLFVTNLFLDLIAENIDLGIRFGELKDSSIVAQRLGKS
VRYLVAAPDYMKGRALPSKPEDLKDHQCVLLNGRNGEAEWHLVSGRKSVRQQVSGPVSSRDFEAVSAFTYRGHGIGLLPS
TYCDDEIRRGELVRLLPDWSSEEIFVHAVYPTRRFLPLRLQVFLEALKAWKTPLWLPLH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=36.2068965517241, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=33.5616438356164, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=31.2714776632302, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789440, Length=288, Percent_Identity=29.5138888888889, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=29.4117647058824, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=28.0276816608997, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=29.4520547945205, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789173, Length=251, Percent_Identity=28.2868525896414, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788706, Length=242, Percent_Identity=25.6198347107438, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1786713, Length=265, Percent_Identity=26.0377358490566, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790011, Length=293, Percent_Identity=21.8430034129693, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=24.9146757679181, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786508, Length=256, Percent_Identity=24.609375, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=30.0699300699301, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33751; Mature: 33620

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLNDIAVFVTVARYGSFSRAAHSLGMPVSTVSRKVTSLEEQLGVTLLQRTTRKLSLTA
CCCHHHHHHEEHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
QGSAYFDQCSEPLAHLIDAEQALTETQRKPEGLLKISVPVIFGQEVFYEFVSAFLKTYPE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECHHHHHHHHHHHHHHCCC
IRIDLFVTNLFLDLIAENIDLGIRFGELKDSSIVAQRLGKSVRYLVAAPDYMKGRALPSK
CEEHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCEEEEEECCCHHCCCCCCCC
PEDLKDHQCVLLNGRNGEAEWHLVSGRKSVRQQVSGPVSSRDFEAVSAFTYRGHGIGLLP
CCCCCCCCEEEEECCCCCCEEEEECCHHHHHHHHCCCCCCCCHHHHHHEEECCCCEECCC
STYCDDEIRRGELVRLLPDWSSEEIFVHAVYPTRRFLPLRLQVFLEALKAWKTPLWLPLH
CCCCCCHHCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure 
ADLNDIAVFVTVARYGSFSRAAHSLGMPVSTVSRKVTSLEEQLGVTLLQRTTRKLSLTA
CCHHHHHHEEHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
QGSAYFDQCSEPLAHLIDAEQALTETQRKPEGLLKISVPVIFGQEVFYEFVSAFLKTYPE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECHHHHHHHHHHHHHHCCC
IRIDLFVTNLFLDLIAENIDLGIRFGELKDSSIVAQRLGKSVRYLVAAPDYMKGRALPSK
CEEHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCEEEEEECCCHHCCCCCCCC
PEDLKDHQCVLLNGRNGEAEWHLVSGRKSVRQQVSGPVSSRDFEAVSAFTYRGHGIGLLP
CCCCCCCCEEEEECCCCCCEEEEECCHHHHHHHHCCCCCCCCHHHHHHEEECCCCEECCC
STYCDDEIRRGELVRLLPDWSSEEIFVHAVYPTRRFLPLRLQVFLEALKAWKTPLWLPLH
CCCCCCHHCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]