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The map label for this gene is ycjZ [C]

Identifier: 209549901

GI number: 209549901

Start: 2374645

End: 2375541

Strand: Reverse

Name: ycjZ [C]

Synonym: Rleg2_2317

Alternate gene names: 209549901

Gene position: 2375541-2374645 (Counterclockwise)

Preceding gene: 209549906

Following gene: 209549900

Centisome position: 52.35

GC content: 61.54

Gene sequence:

>897_bases
GTGGCCCGCGAGAATTTCAATGAGCTGCTGCTTTTCCTGGCGGTGGCGGAGGAGCGCAGCTTCACCAGGGCCGCGGCGAA
ATTGGGGGTCGCGCAATCGACGCTGAGCTACGCCGTCAAGGAACTCGAAGCGCGCATGGGGCTGCGGCTTCTCACCCGCA
CGACGCGGAGCGTGGCGACGACGGAGGCCGGAGAGCGCCTCCGGCAATCGCTTGCACCGCGCATTTCCGAAATCGAGGCG
GATATCGCGGCATTGATGGCCTATCGCGACAAACCGGCCGGCACCGTCAAGATCACCCTTTCAAATCACGCGATGGAAAG
CGTGGTCTGGCCGAAGCTCGCCCCCGTCCTTGGGGAATATCCGGATATCAAACTGGAACTCAGCGTCGACAACAGCTTCC
GCAACATCGTCGAAGATGGCTTCGATGCCGGCGTGCGTCTCGGCGAGAGCCTGGAGAAGGACATGGTTGCGGTGCGGATC
GGGCCGGACTGGCGGCTGGTGGCCGTCGCTTCCCCCGACTATTTCGAGACAAGAGCGGTGCCGACGACACCGCAGGAGCT
GATGCATCACAACTGTATCAACAGGCGACTCGACCGGGTGGGCGGACTGTACGCCTGGGAATTCGAAAAGAACAACGAGG
AGGTCCGGGTGAGGGTGGAGGGCCAGTTCACCTTCAACACCGATCGCGAAATGGTCCACGCTGCCCTCCAAGGCTACGGC
ATCGCCTTCGTTCCGGAAAGCTATGTCGAAACGCACATTGCGGACGGGCGTCTCCAACTCTTCCTCGAAGACTGGACCCC
CAAATTCGCCGGCTATTACCTCTATTACCCCAGCCGGCGGCAGAACCTCCCGGCCTTCTCCGTCATCGTCGATGCGCTTC
GAGAGAGAAATCTCTAG

Upstream 100 bases:

>100_bases
TAGCACCATTCGATAACTCGGATTAGCCGCTGCAATTTGCATGGACTTATCGAAATCGGCTATTAATGGCGCCGGATCGG
AATGACTGGAGACATCAACC

Downstream 100 bases:

>100_bases
CTCCGTCACACCCGCCTCGTCCTTCGAGGCCCCTGTGGGGCACCTCAGGATGAGGCTCTCTTGGAGGCCGCGCGAGCGGT
GGCGCGACAGCAGGCGAGAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MARENFNELLLFLAVAEERSFTRAAAKLGVAQSTLSYAVKELEARMGLRLLTRTTRSVATTEAGERLRQSLAPRISEIEA
DIAALMAYRDKPAGTVKITLSNHAMESVVWPKLAPVLGEYPDIKLELSVDNSFRNIVEDGFDAGVRLGESLEKDMVAVRI
GPDWRLVAVASPDYFETRAVPTTPQELMHHNCINRRLDRVGGLYAWEFEKNNEEVRVRVEGQFTFNTDREMVHAALQGYG
IAFVPESYVETHIADGRLQLFLEDWTPKFAGYYLYYPSRRQNLPAFSVIVDALRERNL

Sequences:

>Translated_298_residues
MARENFNELLLFLAVAEERSFTRAAAKLGVAQSTLSYAVKELEARMGLRLLTRTTRSVATTEAGERLRQSLAPRISEIEA
DIAALMAYRDKPAGTVKITLSNHAMESVVWPKLAPVLGEYPDIKLELSVDNSFRNIVEDGFDAGVRLGESLEKDMVAVRI
GPDWRLVAVASPDYFETRAVPTTPQELMHHNCINRRLDRVGGLYAWEFEKNNEEVRVRVEGQFTFNTDREMVHAALQGYG
IAFVPESYVETHIADGRLQLFLEDWTPKFAGYYLYYPSRRQNLPAFSVIVDALRERNL
>Mature_297_residues
ARENFNELLLFLAVAEERSFTRAAAKLGVAQSTLSYAVKELEARMGLRLLTRTTRSVATTEAGERLRQSLAPRISEIEAD
IAALMAYRDKPAGTVKITLSNHAMESVVWPKLAPVLGEYPDIKLELSVDNSFRNIVEDGFDAGVRLGESLEKDMVAVRIG
PDWRLVAVASPDYFETRAVPTTPQELMHHNCINRRLDRVGGLYAWEFEKNNEEVRVRVEGQFTFNTDREMVHAALQGYGI
AFVPESYVETHIADGRLQLFLEDWTPKFAGYYLYYPSRRQNLPAFSVIVDALRERNL

Specific function: May have a role in the regulation of oprD expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=44.7811447811448, Blast_Score=275, Evalue=2e-75,
Organism=Escherichia coli, GI1787128, Length=278, Percent_Identity=37.7697841726619, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1786401, Length=257, Percent_Identity=29.5719844357977, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789639, Length=298, Percent_Identity=27.8523489932886, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI145693193, Length=284, Percent_Identity=28.8732394366197, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789440, Length=253, Percent_Identity=24.1106719367589, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=27.3972602739726, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790011, Length=269, Percent_Identity=26.7657992565056, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786448, Length=299, Percent_Identity=25.4180602006689, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789173, Length=269, Percent_Identity=26.0223048327138, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=22.8187919463087, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788748, Length=123, Percent_Identity=34.1463414634146, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI157672245, Length=137, Percent_Identity=33.5766423357664, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786713, Length=259, Percent_Identity=28.1853281853282, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787879, Length=153, Percent_Identity=32.6797385620915, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33760; Mature: 33629

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARENFNELLLFLAVAEERSFTRAAAKLGVAQSTLSYAVKELEARMGLRLLTRTTRSVAT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEAGERLRQSLAPRISEIEADIAALMAYRDKPAGTVKITLSNHAMESVVWPKLAPVLGEY
HHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
PDIKLELSVDNSFRNIVEDGFDAGVRLGESLEKDMVAVRIGPDWRLVAVASPDYFETRAV
CCEEEEEEECHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCHHCCCC
PTTPQELMHHNCINRRLDRVGGLYAWEFEKNNEEVRVRVEGQFTFNTDREMVHAALQGYG
CCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEEEEEEECCHHHHHHHHHHCCC
IAFVPESYVETHIADGRLQLFLEDWTPKFAGYYLYYPSRRQNLPAFSVIVDALRERNL
EEECCHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARENFNELLLFLAVAEERSFTRAAAKLGVAQSTLSYAVKELEARMGLRLLTRTTRSVAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEAGERLRQSLAPRISEIEADIAALMAYRDKPAGTVKITLSNHAMESVVWPKLAPVLGEY
HHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
PDIKLELSVDNSFRNIVEDGFDAGVRLGESLEKDMVAVRIGPDWRLVAVASPDYFETRAV
CCEEEEEEECHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCHHCCCC
PTTPQELMHHNCINRRLDRVGGLYAWEFEKNNEEVRVRVEGQFTFNTDREMVHAALQGYG
CCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEEEEEEECCHHHHHHHHHHCCC
IAFVPESYVETHIADGRLQLFLEDWTPKFAGYYLYYPSRRQNLPAFSVIVDALRERNL
EEECCHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043; 1427017 [H]