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The map label for this gene is tcmR [H]

Identifier: 209549892

GI number: 209549892

Start: 2363879

End: 2364481

Strand: Reverse

Name: tcmR [H]

Synonym: Rleg2_2308

Alternate gene names: 209549892

Gene position: 2364481-2363879 (Counterclockwise)

Preceding gene: 209549895

Following gene: 209549879

Centisome position: 52.1

GC content: 59.2

Gene sequence:

>603_bases
ATGGAACAGGATCGACCGAAACAGGAAGGCTGGCGCGAGAAGAAGCGGCGTGAAACCCTTCAGCGCATTAGCGATTCTGC
GCTGAGGCTATTTGCGTCCGATGGCTACGAGGCGACGACGCTGGATGCGATCGCCGAGGCGGCCGGCATTTCGCGGCGGA
CATTCTTCTACTATTTCGACTCGAAAGAGGAGATTCTGGCCGCCTGGCAAAAAGGCCTGCCGGAGGCCTTTCGCGCCGCC
GTTCTGGCGCAGCCGAAAGATCAGTCGCCGCTCGATATGGTCTGCAACGCCCACCTGAAACTTCTGGCGAATTTCGACAC
AGAACAAGCCATCGTCATCGATCGGATTCTTCGCTCCAATGAGCAACTGCGCGCAAGCAACCAGACCAAATACCTGCAGA
TGGAGCAGGTGACTTTTGAAGCGCTGTGCGAAATCTGGCCACACCAGGCGCGCCGCAAGGCGCTGCGCGTGGTGGCGATG
ATGTCCGCCGGCGTGCTGCGGCTTGCGATCGACAGCTGGGCGGAAGAGCAGGGGCGCAAGTCGCTTGCCCACTGTGTAAA
AGAGATGTTTGCCGATTTGAAAGCTGAGCTCGCCCGTGCGTAA

Upstream 100 bases:

>100_bases
GCACTCTTTTCATATCAACCTCGCATCCGTTCACTGCGAAGGTAAATATTACACTGAGTGCAATTTTACAAGAGATGCGA
TTCCTGGTATCGCTGCGGCA

Downstream 100 bases:

>100_bases
AGGCTGACGAGAAAGGCCGGGGCGGCTGAAATTGCTCAAAAAAAGAGAGCATTCCGTTACTTAAACAAAGACGGAAATGC
TCTCATAGTCTGGTGCGCAA

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Tetracenomycin C transcriptional repressor [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MEQDRPKQEGWREKKRRETLQRISDSALRLFASDGYEATTLDAIAEAAGISRRTFFYYFDSKEEILAAWQKGLPEAFRAA
VLAQPKDQSPLDMVCNAHLKLLANFDTEQAIVIDRILRSNEQLRASNQTKYLQMEQVTFEALCEIWPHQARRKALRVVAM
MSAGVLRLAIDSWAEEQGRKSLAHCVKEMFADLKAELARA

Sequences:

>Translated_200_residues
MEQDRPKQEGWREKKRRETLQRISDSALRLFASDGYEATTLDAIAEAAGISRRTFFYYFDSKEEILAAWQKGLPEAFRAA
VLAQPKDQSPLDMVCNAHLKLLANFDTEQAIVIDRILRSNEQLRASNQTKYLQMEQVTFEALCEIWPHQARRKALRVVAM
MSAGVLRLAIDSWAEEQGRKSLAHCVKEMFADLKAELARA
>Mature_200_residues
MEQDRPKQEGWREKKRRETLQRISDSALRLFASDGYEATTLDAIAEAAGISRRTFFYYFDSKEEILAAWQKGLPEAFRAA
VLAQPKDQSPLDMVCNAHLKLLANFDTEQAIVIDRILRSNEQLRASNQTKYLQMEQVTFEALCEIWPHQARRKALRVVAM
MSAGVLRLAIDSWAEEQGRKSLAHCVKEMFADLKAELARA

Specific function: Represses transcription of the divergently oriented tcmR and tcmA (tetracenomycin C resistance and export) genes by binding to an intergenic operator region. This binding is inhibited by tetracenomycin C [H]

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22905; Mature: 22905

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS01081 HTH_TETR_1 ; PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQDRPKQEGWREKKRRETLQRISDSALRLFASDGYEATTLDAIAEAAGISRRTFFYYFD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEC
SKEEILAAWQKGLPEAFRAAVLAQPKDQSPLDMVCNAHLKLLANFDTEQAIVIDRILRSN
CHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
EQLRASNQTKYLQMEQVTFEALCEIWPHQARRKALRVVAMMSAGVLRLAIDSWAEEQGRK
HHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAHCVKEMFADLKAELARA
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEQDRPKQEGWREKKRRETLQRISDSALRLFASDGYEATTLDAIAEAAGISRRTFFYYFD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEC
SKEEILAAWQKGLPEAFRAAVLAQPKDQSPLDMVCNAHLKLLANFDTEQAIVIDRILRSN
CHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
EQLRASNQTKYLQMEQVTFEALCEIWPHQARRKALRVVAMMSAGVLRLAIDSWAEEQGRK
HHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAHCVKEMFADLKAELARA
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1592819; 1592820 [H]