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The map label for this gene is pleD [H]

Identifier: 209549745

GI number: 209549745

Start: 2209683

End: 2211014

Strand: Reverse

Name: pleD [H]

Synonym: Rleg2_2153

Alternate gene names: 209549745

Gene position: 2211014-2209683 (Counterclockwise)

Preceding gene: 209549746

Following gene: 209549742

Centisome position: 48.72

GC content: 59.08

Gene sequence:

>1332_bases
GTGGCTTTTCAAGCGGATTTTCAGCGAGATGGCATCGGGCTCCGTTCGGGTGGGCTCAAGATACTTCTGGTTGAAGATTC
CCGGATGTTTTCCGCTGTGCTCTGCCATCGGTTCCAGACCGAGCTCGGCCTTGCAGTCAAATCCTGCCCGTCGCTGAAGG
CTCTTCGCGAAGAACTTGCCGAGGACGGTCACGGCTACACCATGGCCGTCGTCGATCTCAACCTGCCGGATTCGCCCTAT
GGCGAAGCGCTCGACTGCACGATCGAGCACGATATCCCGGCGATCGTCTTTACCGCGACATTCGATCTCAACACGCGCAA
CAGGATCATGGAGCGCAATGTCATCGATTATGTGCTCAAGGACAATGAATTCGCGCTCGACAATCTGGTCGCCACCGTTC
GGCGAGCGATCTCCAACCGCAAGACGCGGGTGCTCGTCGTCGATGATGTCGTCTCGGTGCGCCAGGTGCTGGTCGACCTC
CTGAAGGCGCAGCAATATCTCGTCGTCGAGGCAAATTCGGGGCTCGAGGCGCTGGCCGCGCTTGAGGCTTACAGCGATAT
CGAACTCGTCGTCACCGACCACCACATGCCCGATATGAGCGGCTACGAGCTGACGCGGCGTATCCGCCATCGCTTCGGTT
CCGACAGACTGCGCGTCATCGGCGTTTCCTCCTCCAACGACCGCATGCTGTCTGCCAGTTTCCTGAAGGCCGGCGCCAGC
GATTTCGTCTACCGTCCCTTCGTCGCCGAGGAATTGCAATGCCGCATCGCCAACAATGCCGAAACGCTGGCGCAGATGCG
TCAGCTGAGGGCGGCGGCGGCCTGCGACTATCTCACAGGTCTCTATAACCGCCGCTATTTCTACGACAACGGCCCGAAGC
TGGTGAACGAGTGCCTGCGGCTGAAAGTGCCGAGTTCGGTGGCCATTCTCGATATCGATCACTTCAAGCGGCTGAACGAC
ACCTATGGCCACGAGATCGGCGACAAGGTGCTGAAGGCTGTCGCAAACAGGCTGTTCACGATCTTCGAGGGCAGCGACAA
TCTTCTCTCGCGGCTTGGCGGTGAAGAATTCGCTATCCTTTTCCCGCAGATGGATTCGGCTGCCGCGACCAAGCTTTGCG
ACGAAATCCGCTCGGACATTTCGCGGCTGAAGGTGACGGCCGACGACGAGGAACTCGGTGTCACCATCTCGATCGGGATC
GCCGAAATCGAGGGCTACGAAACCTTCGAGAACTATCTCAACGCCGCCGACCAGTTCCTCTACATGGCCAAGCACCGAGG
CCGCAACCAAGTCTTTTCCGACGCCAGGATGACGGAAGAAGCGGCGCAATAG

Upstream 100 bases:

>100_bases
TCATTTGTCCCCTTCACGCGCGCAGGCCTCTCGGTTATCGTTCGCCGCGCCGGGGGGAGGGCCGAGTCGGGGCCATCGGA
CGCATGGGGGAATAGCGGCT

Downstream 100 bases:

>100_bases
AGCCATTCTGGCAAGGCGCCGCCCTTTCACTTCAGGCGGCGCGCTCTTTCAAACCCTCAGGCCGCGACCGCCTGTTGACG
CGCAGTCGCCATGGTCATCT

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAFQADFQRDGIGLRSGGLKILLVEDSRMFSAVLCHRFQTELGLAVKSCPSLKALREELAEDGHGYTMAVVDLNLPDSPY
GEALDCTIEHDIPAIVFTATFDLNTRNRIMERNVIDYVLKDNEFALDNLVATVRRAISNRKTRVLVVDDVVSVRQVLVDL
LKAQQYLVVEANSGLEALAALEAYSDIELVVTDHHMPDMSGYELTRRIRHRFGSDRLRVIGVSSSNDRMLSASFLKAGAS
DFVYRPFVAEELQCRIANNAETLAQMRQLRAAAACDYLTGLYNRRYFYDNGPKLVNECLRLKVPSSVAILDIDHFKRLND
TYGHEIGDKVLKAVANRLFTIFEGSDNLLSRLGGEEFAILFPQMDSAAATKLCDEIRSDISRLKVTADDEELGVTISIGI
AEIEGYETFENYLNAADQFLYMAKHRGRNQVFSDARMTEEAAQ

Sequences:

>Translated_443_residues
MAFQADFQRDGIGLRSGGLKILLVEDSRMFSAVLCHRFQTELGLAVKSCPSLKALREELAEDGHGYTMAVVDLNLPDSPY
GEALDCTIEHDIPAIVFTATFDLNTRNRIMERNVIDYVLKDNEFALDNLVATVRRAISNRKTRVLVVDDVVSVRQVLVDL
LKAQQYLVVEANSGLEALAALEAYSDIELVVTDHHMPDMSGYELTRRIRHRFGSDRLRVIGVSSSNDRMLSASFLKAGAS
DFVYRPFVAEELQCRIANNAETLAQMRQLRAAAACDYLTGLYNRRYFYDNGPKLVNECLRLKVPSSVAILDIDHFKRLND
TYGHEIGDKVLKAVANRLFTIFEGSDNLLSRLGGEEFAILFPQMDSAAATKLCDEIRSDISRLKVTADDEELGVTISIGI
AEIEGYETFENYLNAADQFLYMAKHRGRNQVFSDARMTEEAAQ
>Mature_442_residues
AFQADFQRDGIGLRSGGLKILLVEDSRMFSAVLCHRFQTELGLAVKSCPSLKALREELAEDGHGYTMAVVDLNLPDSPYG
EALDCTIEHDIPAIVFTATFDLNTRNRIMERNVIDYVLKDNEFALDNLVATVRRAISNRKTRVLVVDDVVSVRQVLVDLL
KAQQYLVVEANSGLEALAALEAYSDIELVVTDHHMPDMSGYELTRRIRHRFGSDRLRVIGVSSSNDRMLSASFLKAGASD
FVYRPFVAEELQCRIANNAETLAQMRQLRAAAACDYLTGLYNRRYFYDNGPKLVNECLRLKVPSSVAILDIDHFKRLNDT
YGHEIGDKVLKAVANRLFTIFEGSDNLLSRLGGEEFAILFPQMDSAAATKLCDEIRSDISRLKVTADDEELGVTISIGIA
EIEGYETFENYLNAADQFLYMAKHRGRNQVFSDARMTEEAAQ

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=194, Percent_Identity=38.1443298969072, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=34.9693251533742, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=35.5555555555556, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=31.5151515151515, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=35.5555555555556, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=33.9285714285714, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788085, Length=174, Percent_Identity=33.9080459770115, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788956, Length=179, Percent_Identity=34.6368715083799, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=34.5029239766082, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=185, Percent_Identity=31.3513513513514, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788381, Length=178, Percent_Identity=29.7752808988764, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=31.2101910828025, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=32.4675324675325, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49635; Mature: 49504

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFQADFQRDGIGLRSGGLKILLVEDSRMFSAVLCHRFQTELGLAVKSCPSLKALREELA
CCCCCCCCCCCCCEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EDGHGYTMAVVDLNLPDSPYGEALDCTIEHDIPAIVFTATFDLNTRNRIMERNVIDYVLK
HCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHCC
DNEFALDNLVATVRRAISNRKTRVLVVDDVVSVRQVLVDLLKAQQYLVVEANSGLEALAA
CCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHH
LEAYSDIELVVTDHHMPDMSGYELTRRIRHRFGSDRLRVIGVSSSNDRMLSASFLKAGAS
HHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHCCCC
DFVYRPFVAEELQCRIANNAETLAQMRQLRAAAACDYLTGLYNRRYFYDNGPKLVNECLR
CCEECCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHH
LKVPSSVAILDIDHFKRLNDTYGHEIGDKVLKAVANRLFTIFEGSDNLLSRLGGEEFAIL
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHCCCCEEEEE
FPQMDSAAATKLCDEIRSDISRLKVTADDEELGVTISIGIAEIEGYETFENYLNAADQFL
ECCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHH
YMAKHRGRNQVFSDARMTEEAAQ
HHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFQADFQRDGIGLRSGGLKILLVEDSRMFSAVLCHRFQTELGLAVKSCPSLKALREELA
CCCCCCCCCCCCEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EDGHGYTMAVVDLNLPDSPYGEALDCTIEHDIPAIVFTATFDLNTRNRIMERNVIDYVLK
HCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHCC
DNEFALDNLVATVRRAISNRKTRVLVVDDVVSVRQVLVDLLKAQQYLVVEANSGLEALAA
CCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHH
LEAYSDIELVVTDHHMPDMSGYELTRRIRHRFGSDRLRVIGVSSSNDRMLSASFLKAGAS
HHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHCCCC
DFVYRPFVAEELQCRIANNAETLAQMRQLRAAAACDYLTGLYNRRYFYDNGPKLVNECLR
CCEECCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHH
LKVPSSVAILDIDHFKRLNDTYGHEIGDKVLKAVANRLFTIFEGSDNLLSRLGGEEFAIL
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHCCCCEEEEE
FPQMDSAAATKLCDEIRSDISRLKVTADDEELGVTISIGIAEIEGYETFENYLNAADQFL
ECCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHH
YMAKHRGRNQVFSDARMTEEAAQ
HHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA